인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구

이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

녹색건축 전문가 자격제도 개발 연구

이상민,이승민,Lee, Sang Min,Lee, Seung Min 한국교육녹색환경연구원 2014 교육·녹색환경연구 Vol.13 No.1

In an effort to resolve environmental pollution problems, developed countries enforce green building certification systems. The same is true in Korea. However compared professional accreditation system to certification systems did not systematically in Korea. Therefore, in accordance with development of green building related systems and technology the requirement for foster green building professionals is urgent. Accordingly this study is purposed to analyze and propose that green building professional accreditation system development and operation of the direction. Through data analysis domestic and international green building professional accreditation system for draw a development plan. And then conduct an expert Delphi survey reflected in the survey results to development plan.

대식세포주에서 인슐린이 $I{\kappa}B/NF-{\kappa}B$ 경로 활성화에 미치는 영향

이상민,장연실,이춘택,김영환,한성구,심영수,유철규,Lee, Sang-Min,Jang, Yeon-Sil,Lee, Choon-Taek,Kim, Young-Whan,Han, Sung-Koo,Shim, Young-Soo,Yoo, Chul-Gyu 대한결핵및호흡기학회 2010 Tuberculosis and Respiratory Diseases Vol.68 No.3

Background: Sepsis still has a high mortality rate despite adequate supportive care. Newer therapeutic modalities have been developed but they have generally ended in failure. Recently, insulin was reported to have an anti-inflammatory effect by inhibiting the $I{\kappa}B/NF-{\kappa}B$ pathway, and may have therapeutic potential in sepsis. However, the precise mechanism of the anti-inflammatory effect of insulin is unclear. This study examined the role of insulin in activating $I{\kappa}B/NF-{\kappa}B$ in macrophage. Methods: Raw 264.7 cells, a murine macrophage cell line, were used in this experiment. Western blotting using $I{\kappa}B$ Ab and phosphor-specific $I{\kappa}B$ Ab was performed to evaluate the degradation and phosphorylation of $I{\kappa}B$ cells. For the $I{\kappa}B$ Kinase (IKK) activity, an immune complex kinase assay was performed. The level of interleukin-6 (IL-6) was measured by ELISA to determine the level of proinflammatory cytokine. Results: $I{\kappa}B{\alpha}$ degradation began 30 min after lipopolysaccharide (LPS) treatment. However, an insulin pretreatment suppressed the $I{\kappa}B{\alpha}$ degradation caused by the LPS treatment. The phosphorylation of $I{\kappa}B{\alpha}$ and IKK activity was also inhibited by the insulin pretreatment. Finally, the insulin pretreatment showed a tendency to suppress the induction of IL-6 by LPS. Conclusion: Insulin might have an anti-inflammatory effect though partial inhibition of the $I{\kappa}B/NF{\kappa}B$ pathway in macrophage cell lines.

녹비작물을 이용한 윤작이 잡초 제어에 미치는 영향

이상민,이병모,이연,이용환,성좌경,윤홍배,최현석,Lee, Sang-Min,Lee, Byung-Mo,Lee, Youn,Lee, Yong-Hwan,Sung, Jwa-Kyung,Yun, Hong-Bae,Choi, Hyun-Sug 한국유기농업학회 2012 韓國有機農業學會誌 Vol.20 No.2

본 시험은 유기농업 파 및 고추재배를 위하여 호밀과 헤어리베치를 겨울에 윤작하여 잡초 발생량 억제에 미치는 효과를 검토하기 위하여 수행되었다. 파 재배구에서 호밀을 토양에 환원하지 않은 처리구에 비해 호밀을 환원하였을 경우 잡초발생량을 52% 경감시켰다. 고추 재배구에서는 그러한 효과가 뚜렷하게 관찰되지 않았다. 파 재배시 어저귀, 참방동사니, 바랭이와 같은 초종은 호밀뿌리 분비물에 의해 발아가 억제되는 경향이었으나 헤어리베치는 그러한 발생억제 효과가 크지 않았다. 파 재배시 호밀 환원은 잡초발생량을 경감시켰고 잡초의 종 다양성 지수를 증가시켰다. This study was conducted to evaluate the effects of crop rotation on the weed occurrence in green onion and red pepper cultivation. Rye and hairy vetch were annually seeded for winter season. The reduction of weed germination was pronounced by about 50% in green onion field, but was not significant in red pepper field through rye cropping. Among weed species, the germination of Abutilon theophrasti, Cyperus iria and Digitaria ciliaris was distinctly inhibited by exudate of rye root, but not by that of hairy vetch in vitro experiment. The return of rye biomass into onion field decreased amount of weed biomass but increased index of weed diversity.

대장직장 종양에 대한 내시경 점막하 절제술의 연대순 임상 결과와 학습 곡선

이상민 ( Sang Min Lee ),김은수 ( Eun Soo Kim ),박경식 ( Kyung Sik Park ),조광범 ( Kwang Bum Cho ),김동춘 ( Dong Choon Kim ),강유진 ( Yu Jin Kang ),이유진 ( Yoo Jin Lee ),이정민 ( Jung Min Lee ),최은성 ( Eun Sung Choi ),최재혁 ( Ja 대한소화기학회 2014 대한소화기학회지 Vol.64 No.4

Background/Aims: Endoscopic submucosal dissection (ESD) is an effective procedure for en-bloc curative resection of the colorectal tumor. As it requires high technical skills and experience in therapeutic endoscopy, it is important to understand learning curve of ESD technique. The aim of this study was to retrospectively describe the clinical results of ESD and to evaluate learning curve for the ESD of colorectal tumors. Methods: A total of 90 patients with 90 colorectal neoplasms, who had undergone ESD at a tertiary referral hospital from July 2009 to December 2012, were enrolled. The ESD was performed by a single endoscopist. All ESD cases were divided into three periods: first, cases 1-30; second, cases 31-60; and third, cases 61-90. Results: The en-bloc resection rates in third period (100%) was significantly higher than that of the first (93.3%) and second period (80%) (p=0.025). The perforation rate in third period (0%) also significantly decreased compared with that of the first (13.3%) and second period (20%) (p=0.032). To calibrate the difference of tumor size among periods, proficiency was calculated, as the procedure time per specimen area (min/cm2). The proficiency in third period (4.3) was significantly shorter than that of the first (16.8) and second period (10.2) (p=0.004). Conclusions: The learning curve of colorectal ESD in our study shows that at least 60 cases of ESD have to be conducted to acquire sufficient skill of degree without perforation. (Korean J Gastroenterol 2014;64:198-205)

헤어리베치(Vice villosa Roth) 품종별 국내 월동성, Biomass 및 무기태 질소 함량비교

이상민,이연,이용환,성좌경,윤홍배,최현석,Lee, Sang-Min,Lee, Youn,Lee, Yong-Hwan,Sung, Jwa-Kyung,Yun, Hong-Bae,Choi, Hyun-Sug 한국유기농업학회 2013 韓國有機農業學會誌 Vol.21 No.1

헤어리베치 8종의 품종별 국내 적응성 검증을 위해 월동률과 biomass, 그리고 토양 중 무기태질소를 조사하였다. 겨울철 월동 후 생존율은 Hungvillosa와 Ostsaat 품종에서 좋았고, Minnie, Oregon common과 TTF1에서 낮았다. 생존율은 biomass에도 영향을 주어서 Hungvillosa와 Ostsaat 품종에서 가장 높았다. 헤어리베치 품종 간 무기성분 농도는 별다른 차이가 관찰되지 않았다. 토양 중 무기태질소 농도는 질소환원능이 좋았던, Hungvillosa와 Ostsaat가 예초 후 10여일 후에 크게 증가시켰다. This study was conducted to evaluate overwintering of hairy vetch (Vicia villosa Roth), biomass, and inorganic N concentration in soil to verify the adaptability of eight cultivars of hairy vetch into country. Winter survival rate was higher for the hairy vetch cultivars, such as Hungvillosa or Ostsaat, than those of Minnie, Oregon common, and TTF1, which affected amount of biomass of each hairy vetch, with higher biomass observed in Hungvillosa or Ostsaat cultivar. There were no significant difference for the mineral nutrients of each hairy vetch cultivar. Inorganic N concentration in soil was increased at 10 days after mowing by application of Hungvillosa and Ostsaat cultivars that had highly produced N content from the raw materials.

준위상정합 기반의 광매개 처프 펄스 증폭 기술 현황

이상민,Lee, Sang-Min 한국광학회 2009 광학과 기술 Vol.13 No.2

영흥도에서 분리된 Phaeodactylum tricornutum의 증식 및 Monounsaturated fatty acid 관련 지방산 조성 분석

이상민,조용희,신동우,전효남,류영진,임상민,이철균,Lee, SangMin,Cho, Yonghee,Shin, ong-Woo,Jeon, Hyonam,Ryu, YoungJin,Lim, Sang-Min,Lee, Choul-Gyun 한국해양바이오학회 2014 한국해양바이오학회지 Vol.6 No.2

Oxidation stability and cold fuid property are considered as the most important factors for determining biodiesel quality. Among the fatty acids, monounsaturated fatty acid satisfy both oxidation stability and cold flow property of biodiesel quality standards. Microalgae with high monounsaturated fatty acid contents is have some benefit for producing to produce biodiesels with satisfying quality standards. In this study, monounsaturated fatty acid contents of a isolated microalga from Youngheung island was analyzed. Phaeodactylum tricornutum was isolated by streaking, and growth rate and fatty acid composition of the algae were investigated. Total FAME contents were consisted of 26% of saturated fatty acids, 43% of monounsaturated fatty acids, and 18% of polyunsaturated fatty acids. The contents of monounsaturated fatty acid were especially high in the Phaeodactylum species. This result implies that the FAMEs from P. tricornutum may contribute to improve the oxidation stability and cold flow property of biodiesel.

ICA/IRMT 공공기관 기록관리 교육프로그램 학습교재(MPSR)

이상민,Lee, Sang-Min 한국기록학회 2001 기록학연구 Vol.0 No.3

일제시기 총독부 기록과 도시계획 기록의 평가 혹은 재평가 - 이론적 쟁점과 평가의 실제 -

이상민,Lee, Sang-Min 한국기록학회 2006 기록학연구 Vol.0 No.14

이 글에서 필자는 총독부 도시계획기록을 포함한 일제 식민지 시기 역사기록을 어떻게 평가하는 것이 바람직할 것인가 하는 문제의식을 가지고, 이러한 기록에 대해서 여러 가지 평가론을 적용시켜 검토해 보았다. 그럼으로써 상대적으로 유용하고 실제적인 평가 방법론을 도출해 보려고 시도했다. 도시계획기록을 포함한 총독부 기록은 기록학적인 평가 과정을 거치지 않고 당대의 보존기간표에 의해 영구기록으로 결정되어 현재까지 보존되고 있는 기록으로서, 기록을 생산한 조직의 위상과 기능이 명확하게 규명되어 있지 않으며, 기록 생산의 맥락이 대체적으로 불분명하고, 전체 기록이 남아있지 않으며, 그 일부가 우연히 살아남아 보존되기에 이른 것들이다. 총독부 기록과 도시계획기록에 적용시켜본 평가론의 쟁점은 기록의 본원적 가치에 대한 논쟁, 쉘렌버그의 역사기록에 대한 정보가치 평가론, 미래의 이용가치와 경제성의 논리에 기반한 소장 기록 재평가 이론의 유용성, 기능 기반 평가와 도큐멘테이션 전략, 특정한 역사적 시기의 식민지 기록으로서의 특성에 의한 평가, 일제 식민지 기록의 내재적 가치, 당대와 현대의 기록처분권에서 도시계획기록의 "영구 보존기록"으로의 결정 등이었다. 본원적 가치 논쟁에 비추어보면 총독부와 도시계획기록은 오늘날의 대부분의 공공기록과 마찬가지로 진본성과 객관성을 보장받는 기록은 아니다. 생산자의 기록 생산 의도와 진본성을 비판적으로 검토해야 역사적 사료로서 사용될 수 있는 기록이다. 소장기록 재평가론은 경제성 및 효율성의 관점에서 평가를 거치지 않은 기록을 계속해서 더 보존할 필요가 있는가를 판별하는데는 유용하지만 식민지 기록같은 유일성과 희귀성이 있는 기록에는 적용하는 것이 부적절하다. 붐스의 사회 표상화 평가 이론, 즉 기록의 평가 및 선별 준거를 기록의 내적 특성이 아닌 기록이 생산된 사회적 과정과 그 사회를 대표하는 정도에서 찾아야 한다는 접근방식이나 도큐멘테이션 전략, 즉, 당대의 대표적 지표를 선별하고, 개별기관의 범위를 넘어서는 전 사회적 차원의 기록을 평가 선별하고 수집하는 접근 방식이 과거 역사기록인 총독부 기록이나 도시계획기록의 평가에 있어서 실질적으로 유용한 평가틀이 되지는 못했다. 총독부기록은 식민지 통치를 증거하는 얼마 되지 않는 소수의 기록으로서 특정 연대 이전의 기록을 역사기록으로 보존하는 평가 관행으로 볼 때에도 당연히 보존되어야 하는 기록이다. 역사기록으로 결정되는 기년도가 법제화되어 있지는 않지만 관행적으로 보존된 것이 그런 인식을 반영한다고도 볼 수 있다. 총독부 도시계획기록은 색채로 된 도면과 과거의 도시와 가로의 사진 등 실물로 보존해야 할 가치가 있는 것으로 판명되었고 디지털화를 한다고 하더라도 원본을 보존해야 할 필요가 있는 기록으로 평가된다. 총독부 도시계획 기록의 평가는 결과적으로 거시적 기능적 분석론 등 발전된 평가론의 반영 노력에도 불구하고 쉘렌버그식의 역사적 기록의 정보 가치의 평가 방식으로 회귀했다. 역사적 기록이 갖는 정보 가치의 미시적 평가 방식이 위주가 된 것이다. 그러나 동시에 총독부 지방행정기관의 조직의 기능과 활동 내용을 어느 정도까지 재구성해주는 작업이 수행될 수 있었다. 맥락 정보를 잃은 보존기록의 맥락 정보 및 배경 정보를 최대한 재구축하여, 전체 총독부 기록 In this paper, I applied known theories of appraisal and re-appraisal to the Japanese Colonial Archives and the Colonial City Planing Archives in Korea. The purpose of this application to some of sample archives was to develop a useful and effective approach to appraise the archives which were not appraised before they were determined to be "permanent" archives by the Japanese colonial officials. The colonial archives have lost their context and "chain of custody." A large portion of their volume also disappeared. Only thirty thousands volumes survived. The appraisal theories and related issues applied to and tested on these archives are; "original natures" of archives defined by Sir. Hillary Jenkinson, Schellenburg's information value appraisal theory, the re-appraisal theory based on economy of preservation and prospect for use of the archives, function-based appraisal theory and documentation theory, the special nature of the archives as unique, old and rare colonial archives, the intrinsic value of the archives, especially the city planing maps and drawings, and finally, the determination of the city planing archives as permanent archives according to the contemporary and modern disposal authority. The colonial archives tested were not naturally self-proven authentic and trustworthy records as many other archives are. They lost their chain of custody and they do not guarantee the authenticity and sincerity of the producers. They need to be examined and reviewed critically before they are used as historical evidence or any material which documented the contemporary society. Rapport's re-appraisal theory simply does not fit into these rare historical archives. The colonial archives have intrinsic values. Though these archives represent some aspects of the colonial society, they can not document the colonial society since they are just survived remains or a little part of the whole archives created. The functions and the structure of the Government General of Korea(朝鮮總督府) were not fully studied yet and hardly can be used to determine the archival values of the archives created in some parts of the colonial apparatus. The actual appraisal methods proved to be effective in the case of colonial archives was Schellenburg's information value appraisal theory. The contextual and content information of the colonial archives were analysed and reconstructed. The appraisal works also resulted in full descriptions of the colonial archives which were never described before in terms of archival principles.