벼 갈반엽 표지인자 계통의 농업특성 및 SSLP 를 이용한 spl-1, spl-2 유전자의 연관분석

원용재,송문태,김홍열,문헌팔 한국육종학회 2000 한국육종학회지 Vol.32 No.4

벼에서 갈반잎 표지인자 계통의 농업특성 및 SSLP를 이용한 spl-1, spl-2 유전자의 연관 분석

원용재,송문태,김홍열,문헌팔 한국작물학회 2000 한국작물학회 학술발표대회 논문집 Vol.2000 No.1

평야지적응 극조생 내도열병 고품질 벼 신품종 "조운"

원용재,유해영,신영섭,홍하철,김연규,김명기,정국현,전용희,조영찬,안억근,윤광섭 한국육종학회 2010 한국육종학회지 Vol.42 No.3

조운은 농촌진흥청 국립식량과학원에서 벼 수확기의 분산 및 이른 추석에도 안정적으로 생산할 수 있는 평야지적응 조생종을 육성할 목적으로 1996년 조숙이면서 양질 내병 다수성 계통인 SR14880-173-3-3-2-2-2와 초형이 우수하며 수량성이 높은 단간 조생인 운봉20호를 교배하여 2009년 육성한 평야지적응 극조생 단간 내도열병 고품질 벼 품종으로 주요 특성과 수량성을 요약하면 다음과 같다. 1. 보통기 보비재배에서 출수기는 7월 23일로 오대벼보 There are the farmer’s needs to develop early-maturing cultivar adaptable to mid-northern inland plain and alpine area. Furthermore, it is required to develop a rice variety to produce new rice before concentrated marketing dates, even in the years of early Chuseok. ‘Joun’ is a new extremely early-maturing japonica rice cultivar developed in 2009 from the cross of SR14880- 173-3-3-2-2-2/Unbong20 at Cheolwon Substation, National Institute of Crop Science (NICS), Rural Development Administration (RDA). The heading date of ‘Joun’ is July 23 in mid-northern alpine area, which is 7 days earlier than that of Odaebyeo. It has about 61 cm in culm length with semi-erect plant type. Panicle has a few awns and its exertion is good. The number of spikelets per panicle is smaller than that of Odaebyeo and 1,000 grain-weight of brown rice is 21.2 g which is less than 26.3 g of Odaebyeo, but the complete grain ratio is higher. Milled kernels are translucent with non-glutinous endosperm and palatability of cooked rice is good. It shows strong resistance to cold treatment, lodging, premature heading, wilting and viviparous germination during ripening stage. This cultivar shows resistance to leaf blast disease but susceptible to bacterial blight, virus disease and insect pests. The milled rice yield performance of ‘Joun’ is about 5.18 MT/ha by ordinary culture in local adaptability test for three years. This cultivar may be highly adaptable to the mid-northern inland plain and alpine area, north-eastern coastal area and middle plain area.

조생 단간 내도열병 반투명 중간찰 벼 ‘월백’

원용재,신영섭,안억근,김명기,김연규,윤영환,전용희,윤광섭,윤미라,이정희,오명규,정종민,양원하,정국현,황흥구 한국육종학회 2012 한국육종학회지 Vol.44 No.3

‘월백’은 조숙 내병 고품질 중간찰 계통을 육성할 목적으로 2000년 하계에 중간찰 품종인 ‘Milkyqueen’에 조숙 내병 고품질 품종인 ‘그루벼’를 인공교배하여 2010년 육성된 조생 내도복 반투명 중간찰 품종으로 주요 특성을 요약하면 다음과 같다. ‘월백’의 보통기 보비재배에서 출수기는 평균 7월 31일로 ‘오대벼’보다 1일 늦은 조생종이다. ‘월백’의 간장은 67 cm이며, 주당수수는 16개, 수당립수는 59개이고, 현미천립중은 21.4 g 으로 ‘오대벼’보다 가볍다. ‘월백’의 아밀로스함량은 11.6%로 중간찰이고 쌀 외관은 투명도가 4로 반투명해 보인다. ‘월백’은 위조에 강하고 불시출수가 잘 되지 않으며 성숙기에 하엽 노화가 느린 편이다. ‘월백’은 수발아에 강한 편이고, 내랭성은 ‘오대벼’와 비슷한 수준이다. ‘월백’은 잎도열병에는 강한 저항성이고 흰잎마름병, 바이러스병 및 해충에는 약하다. 지역적응시험 보통기 보비재배에서 평균 쌀 수량이 5.13 MT/ha로 ‘오대벼’ 대비 95%의 수량성을 보였다. ‘월백’의 재배적지는 북부평야지, 중산간지, 남부고랭지 및 동북부해안지이다. ‘Wolbaek’ is an early-maturing, semi-transparent low amylose content variety developed from a cross between ‘Milkyqueen’ and ‘Geuroobyeo’ by the rice breeding team of National Institute of Crop Science, Rural Development Administration (RDA) in 2000. ‘Wolbaek’ has about 102 days duration from seeding to heading in middle plain, mid-northern inland plain, southern alpine and north-eastern coastal areas. It is about 67 cm in culm length with semi-erect plant type and good canopy architecture. ‘Wolbaek’ has 16 tillers per hill and 59 spikelets per panicle. Its 1,000 grain-weight of brown rice is 21.4 g which is less than that of ‘Odaebyeo’ (26.4 g). The milled kernels look like semi-transparent dull because amylose content of this variety is about 11.6%. This variety shows strong resistance to cold treatment, lodging and wilting. ‘Wolbaek’ shows resistant to blast disease but susceptible to bacterial blight, stripe virus and insect pests. The milled rice yield performance of this variety is about 5.13 MT/ha by ordinary culture in local adaptability test for three years. ‘Wolbaek’ may be highly adaptable to the middle plain area, mid-northern inland plain area, southern alpine area and north-eastern coastal areas of Korea.

극조생 내도열병 다수성 찰벼 ‘청백찰’

원용재,신영섭,안억근,김명기,김연규,이점호,전용희,정국현,양창인,윤영환,조영찬,천아름,이상복,오명규,정희채,현웅조,정응기 한국육종학회 2013 한국육종학회지 Vol.45 No.4

조숙 내병 고품질 찰벼를 육성할 목적으로 2000년 하계에 조숙 내도열병 내도복성인 SR20261-5-4-10 계통을 모본으로 조생찰벼인 진부찰을 인공교배하여 SR26688-1-2-2 계통을 선발하여 철원77호로 계통명을 부여하였다. 2008~2010년 3 개년 간 지역적응시험 실시결과 그 우수성이 인정되어 2010 년 12월 직무육성 신품종 선정위원회에서 국가목록등재 품종 으로 선정됨과 동시에 ‘청백찰’로 명명되었다. ‘청백찰’의 출 수기는 보통기 재배에서 평균 출수기가 7월27일로 ‘오대’보 다 4일 빠른 극조생종이다. 벼키는 68 cm로 ‘오대’의 69 cm 와 비슷하며, 이삭길이와 주당 이삭수도 19 cm와 14개로 ‘오 대’와 비슷하나 이삭당 입수는 80개로 ‘오대’보다 많으며, 등 숙율은 78.5%로 ‘오대’보다 약간 낮고, 현미 천립중이 22.3 g으로 중립종이다. ‘청백찰’은 도열병저항성은 ‘오대’보다 강 하나, 흰잎마름병, 줄무늬잎마름병 및 벼멸구에 대한 저항성 이 없다. ‘청백찰’은 불시출수 현상은 ‘오대’와 비슷하였고 위 조현상은 없고, 내랭성검정 결과 내랭성이 ‘오대’와 비슷한 정도를 보였으며, 도복에 강한 편이다. ‘청백찰’의 제현율은 80.8%로 ‘오대’(82.5%) 보다 약간 낮고, 현미 장폭비가 1.86 으로 중단원립이다. 백미외관은 찹쌀이므로 뽀얗게 보이고, 알칼리 붕괴도는 ‘오대’의 6.7에 비해 5.5로 낮으며 단백질함 량은 7.1%로 ‘오대’의 6.7보다 약간 높다. 북부평야, 중산간 지 및 남부 고랭지 5개소, 동북부 해안 1개소, 중북부 고랭지 1개소에서 보통기 보비재배 평균 쌀수량은 5.35 MT/ha로 대 비품종 ‘오대’의 5.39 MT/ha과 비슷한 수량성을 보였다. ‘청 백찰’의 재배적지는 북부평야, 중산간지, 남부 및 중북부 고랭 지 및 동북부 해안지이다. The ‘Cheongbaekchal’, a glutinous rice (Oryza sativa L.) cultivar, was developed by the rice breeding team of National Institute of Crop Science, RDA, in 2010. It was derived from a cross between SR20261-5-4-10 and ‘Jinbuchal’ which is an extremely early maturing variety with northern alpine area adaptability. The heading date of ‘Cheongbaekchal’ is July 27 that is 4 days earlier than that of ‘Odae’. This cultivar has 68 cm of culm length, 14 tillers per hill and 80 spikelets per panicle that is more than that of ‘Odae’. This variety showed similar cold tolerance to ‘Odae’ while exposed to cold stress. This variety showed resistance to blast disease but susceptible to bacterial blight, stripe virus and planthoppers. The milled rice of this variety exhibits opaque and glutinous endosperm and short grain shape. This variety has lower Alkali digestive value (5.5) than that of ‘Odae’. The dehulling recovery rate of ‘Cheongbaekchal’ is lower than those of ‘Odae’. The milled rice yield performance of ‘Cheongbaekchal’ was 5.35 MT/ha in local adaptability test for three years. ‘Cheongbaekchal’ is adaptable to northern plain and mid-mountainous, southern alpine and north-eastern coastal area of Korea.

벼 및 몇가지 내염성 초종에서 염처리시 양이온 함량과 내염성의 관계

원용재,허문회,고희종 한국작물학회 1991 한국작물학회 학술발표대회 논문집 Vol.1991 No.-

형질전환 벼 이용 품종 조기개발 체계 확립

원용재,양세준,남민희,이기환,한창덕,김순철,조준현,고종민,박향미 한국식물생명공학회 2004 JOURNAL OF PLANT BIOTECHNOLOGY Vol.31 No.1

This study was carried out to establish a new breeding scheme which is connected with conventional breeding method and anther culture method. To develop a herbicide resistant and direct seeding rice, F1 plants were subjected to anther culture and regenerated plants from 5 crosses were studied to confirm the introduction of bar gene. After PCR analysis, we selected 227 plants which were carrying herbicide resistance gene (bar) out of 1,508 regenerated plants from anther culture. Among 169 A2 lines carrying herbicide resistant gene from 5 crosses including YR23235 (Dongjin Ds3(BarR)/ Milyang165), 42 lines that had superior agronomic characters were selected for further research. Among them, YR23235Acp79 which showed herbicide resistance, direct seeding adaptability and superiority in major agronomic characters was named Milyang 204. This breeding scheme proved that the anther culture of F1 plants crossed between transformant and cultivar or transformant alone could be utilized in breeding programs for a rapid progeny fixation and development of a variety. 형질전환 식물체를 직접 이용하기에는 후대에 유전적인 고 정 및 포장검정이라는 문제가 대두되는데, 이를 해결하기 위하여 본 연구는 바스타저항성 유전자가 도입된 벼와 직파적 응성 계통을 교잡한 후 약배양에 의한 형질전환체 고정 등 기 존 육종체계와 연계하여 제초제 저항성 직파적응 계통을 조 기에 육성할 수 있는 체계를 확립하고자 수행하였다. 제초제 저항성 직파적응 계통을 조기에 육성하고자 1999년 동계에 교배된 동진벼 Ds15 (BarR)/주남벼 등 5조합으로부터 2000년 F1을 양성하고, 이들을 PCR 분석으로 제초제 저항성 유전자 bar 도입을 확인한 후 약배양을 실시하여 얻어진 1,508개의 재분화 식물체 중 제초제 저항성 유전자가 도입된 169개체 (A2)에서 채종하였다. 이들을 2001년 포장에 공시하여 42계통 을 선발하고, 2002년에 동진벼 Ds15 (BarR)/주남벼 조합에서 수량 등 주요 농업형질이 우수한 7계통을 생산력 검정시험에 공시한 결과 제초제 저항성이면서 직파적응성 등 기타 농업 형질이 우수한 YR23235Acp79를 선발하여 밀양 204호로 계 통명을 부여하였다. 결과적으로 형질전환체를 이용하여 교배 부터 계통선발까지 3년 만에 목적하는 계통을 조기에 육성할 수 있는 생력육종체계가 확립되었다.

벼 폐화수정 특성의 유전과 다른 농업형질과의 관계

원용재,고희종,허문희 한국육종학회 1998 한국육종학회지 Vol.30 No.1

건식 쌀가루 특성이 개선된 ‘미시루’의 농업적 특성

원용재,안억근,정국현,홍하철,현웅조,박향미,윤미라,이정희,서정필,정응기,장재기,곽지은,오세관 한국육종학회 2020 한국육종학회지 Vol.52 No.2

The rice cultivar ‘Misiru’, which is ideal for rice flour production by dry milling, was developed by the rice breeding team atthe National Institute of Crop Science (NICS), Rural Development Administration (RDA), Korea, in 2017 to vitalize the rice processing industry. It is derived from a cross between ‘Daeripbyeo1’ and ‘Seolgaeng’. ‘Misiru’ was grown on a middle plain area by ordinary cultivation methods. The heading date was on August 13, culm length was 85 cm, and there were 12 panicles and 112 spikelets per panicle. The ripened grainratio was lower than that of ‘Hwaseong’; however, the 1,000 brown rice weight was 30.8 g, about 8 g heavier than that of ‘Hwaseong’. ‘Misiru’ showed medium resistance to blast but susceptibility to bacterial blight, viruses, and insect pests. It was resistant to lodging, whereaspre-harvest sprouting rate and cold tolerance were similar to those of ‘Hwaseong’. The milled rice appeared non-glutinous opaque, with opaquegrain rate of 83.7%. The rice grain was soft and starch granules in the endosperm were of spherical type, similar to wheat. The grain contained6.4% protein and 18.6% amylose (the content of the latter was similar to that in ‘Hwaseong’). However, its milled rice recovery rate waslower than that of ‘Hwaseong’. The average size of dry-milling rice flour was 64.4 μm, and the rate of damaged starch was 5.2%, lowerthan that of ‘Hwaseong’. ‘Misiru’ milled rice yield was 5.66 MT/ha, 8% higher than the yield potential of ‘Hwaseong’. The cultivar ‘Misiru’is thus suitable for rice flour production by dry milling (Grant No. 7682).

열대아시아 적응 다수성 자포니카 벼 ‘아세미1호’

원용재,정응기,강경호,홍하철,조영찬,정오영,장재기,양운호,정국현,여운상,서대하,성낙식,이점호,김보경 한국육종학회 2019 한국육종학회지 Vol.51 No.2

Asemi1' is a rice variety derived from a cross between ‘IR71667’, which has a Jinmibyeo background with a long basic vegetative phase, and ‘IR77863’, which has a Shinunbongbyeo background with a high yield and early maturity, by the rice breeding team at NICS, RDA, in 2014. The heading date of ‘Asemi1’ is July 29, 14 days earlier than that of the check variety ‘Hwaseong’. It has a culm length of 84 cm and 110 spikelets per panicle. ‘Asemi1’ is resistant to blast disease, but susceptible to other viruses and planthoppers. The milled rice of this variety exhibits translucent, clear non-glutinous endosperm and a short grain shape. The protein content (7.3%) was higher than that of ‘Hwaseong’, and the amylose content (19.6%) was similar to that of ‘Hwaseong’. The milled rice recovery rate of ‘Asemi1’ was similar to that of ‘Hwaseong’. However, the head rice rate of ‘Asemi1’ was lower than that of ‘Hwaseong’. The milled rice yield of ‘Asemi1’ was 5.92 MT/ha, which was higher than that of ‘Hwaseong’ in ordinary cultivation. ‘Asemi1’ was suitable for adaptation to a wide region of tropical Asia (Registration No. 6809).