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혈액투석 환자의 빈혈치료에 있어 epoetin alfa와 darbepoetin alfa의 비교 연구
박향미,김재연,신혜영,곽혜선 한국병원약사회 2007 병원약사회지 Vol.24 No.1
Anemia is a common complication in chronic kidney disease (CKD) patients. As erythropoiesis-stimulating agents, Epoetin alfa (EPO) and darbepoetin alfa (DARB) are used to treatment of anemia in CKD patients. Especially, DARB has three times of half-time compare with EPO. The purpose of this study is to estimate the efficacy of EPO and DARB for treatment of anemia in hemodialysis patients. Data were collected retrospectively from patients who have been received hemodialysis in Asan Medical Center between January 1, 2002 and June 30, 2006. Eligible patients were 18 years of age or older and baseline Hb≤11g/dL. End points were the change of Hb level in 4, 8, 12 weeks from baseline, Hb response rate, and drug cost of EPO and DARB. Both all dose and common dose (EPO: 4000U/TIW, DARB: 60mcg/QW), the mean change in Hb level from baseline to 4, 8, 12 weeks was larger with EPO than DARB, but not significantly different(p>0.05). Combination therapy with iron supplementation, the mean change in Hb level was significantly larger with EPO than with DARB at 4 weeks (p<0.01). Hb response rate (≥11g/dL) was more common with EPO than DARB, while drug cost was higher for DARB than EPO. The mean changes in Hb level with EPO and DARB were not significantly different except for combination therapy with iron supplementation. Therefore when choosing drugs, considering merits and demerits of two drugs or patient's condition will be desirable.
GBSSI 유전자 3' UTR 영역의 발현 억제 dsRNAi 벡터를 이용한 아밀로스함량 조절 벼 개발
박향미,최만수,천아름,이정희,김명기,김연규,신동범,이장용,김율호 한국육종학회 2010 한국육종학회지 Vol.42 No.5
본 연구에서는 RNAi 기작을 이용하여 식미에 중요한 영향을 미치는 아밀로스 함량을 다양화하기 위해 GBSSI 유전자의 3’-UTR 부위를 targeting하여 dsRNA를 생성시킬 수 있는 운반체를 제작하고, 벼에 형질전환 하였다. 작성된 형질전환체들을 대상으로 I2-KI 용액 반응과 아밀로스 함량을 분석한 결과, I2-KI 용액에 대한 반응은 waxy 타입으로 나타났으나 아밀로스 함량은 찰벼와 저아밀로스 벼 사이에 해당되는 범위를 보였다. 원품종과 The amylose content of starch is a major factor in the texture of cooked cereal grains. Therefore, down-regulation of amylose synthesis is one of the alternative method to improve eating quality of rice. We developed transgenic rice plants designed to suppress granule-bound starch synthase I(GBSSI) gene using RNA interference(RNAi) technology. Transgenic plants with RNAi vector containing the 3’-UTR region of GBSSI showed a lower amylose content in rice endosperm than that of wild-type. The range of amylose content was 5.9~9.0% in the transgenic plants, whereas that of wild-type was 17.7~18.0%. Transgenic rices showed the decrease of short chain and the increase of long chain by analyzing chain length distribution of amylopectin in the endosperm. In the SEM micrographs, we found that compound starch granules in whole grains of the wild-type rice were readily split during fracturing, while the starch granules in RNAi-transgenic lines showed small voluminous, non-angular rounded bodies.