벼 갈반엽 표지인자 계통의 농업특성 및 SSLP 를 이용한 spl-1, spl-2 유전자의 연관분석

원용재,송문태,김홍열,문헌팔 한국육종학회 2000 한국육종학회지 Vol.32 No.4

건식 쌀가루 특성이 개선된 ‘미시루’의 농업적 특성

원용재,안억근,정국현,홍하철,현웅조,박향미,윤미라,이정희,서정필,정응기,장재기,곽지은,오세관 한국육종학회 2020 한국육종학회지 Vol.52 No.2

The rice cultivar ‘Misiru’, which is ideal for rice flour production by dry milling, was developed by the rice breeding team atthe National Institute of Crop Science (NICS), Rural Development Administration (RDA), Korea, in 2017 to vitalize the rice processing industry. It is derived from a cross between ‘Daeripbyeo1’ and ‘Seolgaeng’. ‘Misiru’ was grown on a middle plain area by ordinary cultivation methods. The heading date was on August 13, culm length was 85 cm, and there were 12 panicles and 112 spikelets per panicle. The ripened grainratio was lower than that of ‘Hwaseong’; however, the 1,000 brown rice weight was 30.8 g, about 8 g heavier than that of ‘Hwaseong’. ‘Misiru’ showed medium resistance to blast but susceptibility to bacterial blight, viruses, and insect pests. It was resistant to lodging, whereaspre-harvest sprouting rate and cold tolerance were similar to those of ‘Hwaseong’. The milled rice appeared non-glutinous opaque, with opaquegrain rate of 83.7%. The rice grain was soft and starch granules in the endosperm were of spherical type, similar to wheat. The grain contained6.4% protein and 18.6% amylose (the content of the latter was similar to that in ‘Hwaseong’). However, its milled rice recovery rate waslower than that of ‘Hwaseong’. The average size of dry-milling rice flour was 64.4 μm, and the rate of damaged starch was 5.2%, lowerthan that of ‘Hwaseong’. ‘Misiru’ milled rice yield was 5.66 MT/ha, 8% higher than the yield potential of ‘Hwaseong’. The cultivar ‘Misiru’is thus suitable for rice flour production by dry milling (Grant No. 7682).

열대아시아 적응 다수성 자포니카 벼 ‘아세미1호’

원용재,정응기,강경호,홍하철,조영찬,정오영,장재기,양운호,정국현,여운상,서대하,성낙식,이점호,김보경 한국육종학회 2019 한국육종학회지 Vol.51 No.2

Asemi1' is a rice variety derived from a cross between ‘IR71667’, which has a Jinmibyeo background with a long basic vegetative phase, and ‘IR77863’, which has a Shinunbongbyeo background with a high yield and early maturity, by the rice breeding team at NICS, RDA, in 2014. The heading date of ‘Asemi1’ is July 29, 14 days earlier than that of the check variety ‘Hwaseong’. It has a culm length of 84 cm and 110 spikelets per panicle. ‘Asemi1’ is resistant to blast disease, but susceptible to other viruses and planthoppers. The milled rice of this variety exhibits translucent, clear non-glutinous endosperm and a short grain shape. The protein content (7.3%) was higher than that of ‘Hwaseong’, and the amylose content (19.6%) was similar to that of ‘Hwaseong’. The milled rice recovery rate of ‘Asemi1’ was similar to that of ‘Hwaseong’. However, the head rice rate of ‘Asemi1’ was lower than that of ‘Hwaseong’. The milled rice yield of ‘Asemi1’ was 5.92 MT/ha, which was higher than that of ‘Hwaseong’ in ordinary cultivation. ‘Asemi1’ was suitable for adaptation to a wide region of tropical Asia (Registration No. 6809).

[PE-0046] 조생 친환경재배 가능 복합내병성 고품질 벼 ‘철원110호’ 특성

원용재,안억근,현웅조,정국현,이윤승,김정주,곽지은,구본일,최원영,박향미 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

[PE-0045] 북방지역 적응 준조생 내수발아 다수성 벼 ‘철원108호’ 특성

원용재,정국현,현웅조,안억근,이윤승,김정주,곽지은,구본일,최원영,박향미 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

극조생 내도열병 다수성 찰벼 ‘청백찰’

원용재,신영섭,안억근,김명기,김연규,이점호,전용희,정국현,양창인,윤영환,조영찬,천아름,이상복,오명규,정희채,현웅조,정응기 한국육종학회 2013 한국육종학회지 Vol.45 No.4

조숙 내병 고품질 찰벼를 육성할 목적으로 2000년 하계에 조숙 내도열병 내도복성인 SR20261-5-4-10 계통을 모본으로 조생찰벼인 진부찰을 인공교배하여 SR26688-1-2-2 계통을 선발하여 철원77호로 계통명을 부여하였다. 2008~2010년 3 개년 간 지역적응시험 실시결과 그 우수성이 인정되어 2010 년 12월 직무육성 신품종 선정위원회에서 국가목록등재 품종 으로 선정됨과 동시에 ‘청백찰’로 명명되었다. ‘청백찰’의 출 수기는 보통기 재배에서 평균 출수기가 7월27일로 ‘오대’보 다 4일 빠른 극조생종이다. 벼키는 68 cm로 ‘오대’의 69 cm 와 비슷하며, 이삭길이와 주당 이삭수도 19 cm와 14개로 ‘오 대’와 비슷하나 이삭당 입수는 80개로 ‘오대’보다 많으며, 등 숙율은 78.5%로 ‘오대’보다 약간 낮고, 현미 천립중이 22.3 g으로 중립종이다. ‘청백찰’은 도열병저항성은 ‘오대’보다 강 하나, 흰잎마름병, 줄무늬잎마름병 및 벼멸구에 대한 저항성 이 없다. ‘청백찰’은 불시출수 현상은 ‘오대’와 비슷하였고 위 조현상은 없고, 내랭성검정 결과 내랭성이 ‘오대’와 비슷한 정도를 보였으며, 도복에 강한 편이다. ‘청백찰’의 제현율은 80.8%로 ‘오대’(82.5%) 보다 약간 낮고, 현미 장폭비가 1.86 으로 중단원립이다. 백미외관은 찹쌀이므로 뽀얗게 보이고, 알칼리 붕괴도는 ‘오대’의 6.7에 비해 5.5로 낮으며 단백질함 량은 7.1%로 ‘오대’의 6.7보다 약간 높다. 북부평야, 중산간 지 및 남부 고랭지 5개소, 동북부 해안 1개소, 중북부 고랭지 1개소에서 보통기 보비재배 평균 쌀수량은 5.35 MT/ha로 대 비품종 ‘오대’의 5.39 MT/ha과 비슷한 수량성을 보였다. ‘청 백찰’의 재배적지는 북부평야, 중산간지, 남부 및 중북부 고랭 지 및 동북부 해안지이다. The ‘Cheongbaekchal’, a glutinous rice (Oryza sativa L.) cultivar, was developed by the rice breeding team of National Institute of Crop Science, RDA, in 2010. It was derived from a cross between SR20261-5-4-10 and ‘Jinbuchal’ which is an extremely early maturing variety with northern alpine area adaptability. The heading date of ‘Cheongbaekchal’ is July 27 that is 4 days earlier than that of ‘Odae’. This cultivar has 68 cm of culm length, 14 tillers per hill and 80 spikelets per panicle that is more than that of ‘Odae’. This variety showed similar cold tolerance to ‘Odae’ while exposed to cold stress. This variety showed resistance to blast disease but susceptible to bacterial blight, stripe virus and planthoppers. The milled rice of this variety exhibits opaque and glutinous endosperm and short grain shape. This variety has lower Alkali digestive value (5.5) than that of ‘Odae’. The dehulling recovery rate of ‘Cheongbaekchal’ is lower than those of ‘Odae’. The milled rice yield performance of ‘Cheongbaekchal’ was 5.35 MT/ha in local adaptability test for three years. ‘Cheongbaekchal’ is adaptable to northern plain and mid-mountainous, southern alpine and north-eastern coastal area of Korea.

벼 폐화수정 특성의 유전과 다른 농업형질과의 관계

원용재,고희종,허문희 한국육종학회 1998 한국육종학회지 Vol.30 No.1

형질전환 벼 이용 품종 조기개발 체계 확립

원용재,이기환,조준현,고종민,박향미,한창덕,양세준,김순철,남민희,Won, Yong-Jae,Yi, Gi-Hwan,Cho, Jun-Hyeon,Ko, Jong-Min,Park, Hyang-Mi,Han, Chang-Deok,Yang, Sae-Jun,Kim, Soon-Chul,Nam, Min-Hee 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.1

This study was carried out to establish a new breeding scheme which is connected with conventional breeding method and anther culture method. To develop a herbicide resistant and direct seeding rice, $F_1$ plants were subjected to anther culture and regenerated plants from 5 crosses were studied to confirm the introduction of bar gene. After PCR analysis, we selected 227 plants which were carrying herbicide resistance gene (bar) out of 1,508 regenerated plants from anther culture. Among 169 $A_2$ lines carrying herbicide resistant gene from 5 crosses including YR23235 (Dongjin Ds3(Ba $r^{R}$)/ Milyang165), 42 lines that had superior agronomic characters were selected for further research. Among them, YR23235Acp79 which showed herbicide resistance, direct seeding adaptability and superiority in major agronomic characters was named Milyang 204. This breeding scheme proved that the anther culture of $F_1$ plants crossed between transformant and cultivar or transform ant alone could be utilized in breeding programs for a rapid progeny fixation and development of a variety.y. 형질전환 식물체를 직접 이용하기에는 후대에 유전적인 고정 및 포장검정이라는 문제가 대두되는데, 이를 해결하기 위하여 본 연구는 바스타저항성 유전자가 도입된 벼와 직파적 응성 계통을 교잡한 후 약배양에 의한 형질전환체 고정 등 기존 육종체계와 연계하여 제초제 저항성 직파적응 계통을 조기에 육성할 수 있는 체계를 확립하고자 수행하였다. 제초제 저항성 직파적응 계통을 조기에 육성하고자 1999년 동계에 교배된 동진벼 Ds15(Ba $r^{R}$)/주남벼 등 5조합으로부터 2000년 $F_1$을 양성하고, 이들을 PCR분석으로 제초제 저항성 유전자 bar도입을 확인한 후 약배양을 실시하여 얻어진 1,508개의 재분화 식물체 중 제초제 저항성 유전자가 도입된 169개체 ( $A_2$)에서 채종하였다. 이들을 2001년 포장에 공시하여 42계통을 선발하고,2002년에 동진벼 Ds15 (Ba $r^{R}$)/주남벼 조합에서 수량 등 주요 농업형질이 우수한 7계통을 생산력 검정시험에 공시한 결과 제초제 저항성이면서 직파적응성 등 기타 농업형질이 우수한 YR23235Acp79를 선발하여 밀양 204호로 계통명을 부여하였다. 결과적으로 형질전환체를 이용하여 교배부터 계통선발까지 3년 만에 목적하는 계통을 조기에 육성할 수 있는 생력육종체계가 확립되었다.

형질전환 벼 이용 품종 조기개발 체계 확립

원용재,양세준,남민희,이기환,한창덕,김순철,조준현,고종민,박향미 한국식물생명공학회 2004 JOURNAL OF PLANT BIOTECHNOLOGY Vol.31 No.1

This study was carried out to establish a new breeding scheme which is connected with conventional breeding method and anther culture method. To develop a herbicide resistant and direct seeding rice, F1 plants were subjected to anther culture and regenerated plants from 5 crosses were studied to confirm the introduction of bar gene. After PCR analysis, we selected 227 plants which were carrying herbicide resistance gene (bar) out of 1,508 regenerated plants from anther culture. Among 169 A2 lines carrying herbicide resistant gene from 5 crosses including YR23235 (Dongjin Ds3(BarR)/ Milyang165), 42 lines that had superior agronomic characters were selected for further research. Among them, YR23235Acp79 which showed herbicide resistance, direct seeding adaptability and superiority in major agronomic characters was named Milyang 204. This breeding scheme proved that the anther culture of F1 plants crossed between transformant and cultivar or transformant alone could be utilized in breeding programs for a rapid progeny fixation and development of a variety. 형질전환 식물체를 직접 이용하기에는 후대에 유전적인 고 정 및 포장검정이라는 문제가 대두되는데, 이를 해결하기 위하여 본 연구는 바스타저항성 유전자가 도입된 벼와 직파적 응성 계통을 교잡한 후 약배양에 의한 형질전환체 고정 등 기 존 육종체계와 연계하여 제초제 저항성 직파적응 계통을 조 기에 육성할 수 있는 체계를 확립하고자 수행하였다. 제초제 저항성 직파적응 계통을 조기에 육성하고자 1999년 동계에 교배된 동진벼 Ds15 (BarR)/주남벼 등 5조합으로부터 2000년 F1을 양성하고, 이들을 PCR 분석으로 제초제 저항성 유전자 bar 도입을 확인한 후 약배양을 실시하여 얻어진 1,508개의 재분화 식물체 중 제초제 저항성 유전자가 도입된 169개체 (A2)에서 채종하였다. 이들을 2001년 포장에 공시하여 42계통 을 선발하고, 2002년에 동진벼 Ds15 (BarR)/주남벼 조합에서 수량 등 주요 농업형질이 우수한 7계통을 생산력 검정시험에 공시한 결과 제초제 저항성이면서 직파적응성 등 기타 농업 형질이 우수한 YR23235Acp79를 선발하여 밀양 204호로 계 통명을 부여하였다. 결과적으로 형질전환체를 이용하여 교배 부터 계통선발까지 3년 만에 목적하는 계통을 조기에 육성할 수 있는 생력육종체계가 확립되었다.

건식 쌀가루 전용 뽀얀메 ‘한가루’

원용재,안억근,정응기,장재기,이정희,정국현,현웅조,조영찬,오세관,윤미라,김보경,김병주 한국육종학회 2019 한국육종학회지 Vol.51 No.2

The ‘Hangaru’ is a rice variety derived from a cross between ‘Seolgaeng’, which is suitable for making rice wine, and ‘Daeripbyeo1’, which has a heavy grain weight (34.8g) and is suitable for deep fried rice, by the rice breeding team at the National Institute of Crop Science, Rural Development Administration, in 2017. The heading date of ‘Hangaru’ was August 18 and was 10 days later than check variety, ‘Hwaseong’, in Suwon. It had a culm length of 79 cm and 102 spikelets per panicle, and showed similar tolerance to ‘Hwaseong’ when exposed to cold stress. ‘Hangaru’ showed medium resistance to blast disease, but susceptibility to bacterial blight, viruses and brown planthoppers. The milled rice of this variety exhibited an opaque and non-glutinous endosperm. The 1,000 grain weight of brown rice was 32.9 g, which was 10.8 g heavier than that of ‘Hwaseong’; the protein content was 7.0% and the amylose content (19.2%) was similar to that of ‘Hwaseong’. The milled rice recovery rate was lower than that of ‘Hwaseong’. However, opaque grain percentage of milled rice was 88.1%. The hardness of the rice grain was soft and the starch granule shape in the endosperm was round, similar to wheat. The average size of rice flour was 71.0 µm and the damaged starch rate was 6.4%. These results showed that this variety could be suitable for dry milling rice flour production. ‘Hangaru’ had a yield of 5.42 MT/ha of milled rice productivity in ordinary cultivation, which was 99% of that of ‘Hwaseong’. ‘Hangaru’ was adaptable to the middle plain area and Mid-west coast of Korea (Registration No. 7270).