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자포니카 및 통일형 벼 품종에서의 식미 관련 저장단백질 특성
곽지은,이점식,윤미라,김미정,천아름,이춘기 한국작물학회 2016 한국작물학회지 Vol.61 No.4
In this study, we analyzed seed storage proteins in order to investigate the main factors related to the eating quality of japonica and tongil-type rice varieties. Sensory evaluation was performed by a trained panel to assess the appearance (color and glossiness), flavor, taste, stickiness, texture, and overall score of nine japonica and three tongil-type rice cultivars. Moreover, the pattern of variation in rice storage proteins was examined by electrophoresis of protein extracts. The electrophoretic pattern of rice proteins showed 16.4 kDa albumin, 26.4 kDa globulin, 34-39 kDa and 21-22 kDa glutelin, and 14.3 kDa prolamin. In terms of storage protein, the varietal differences between japonica and tongil-type rice were found in albumin, globulin, and the α-1, and α-2 sub-units of acidic glutelin. Furthermore, the overall sensory evaluation score was observed to be positively correlated with albumin (0.495 ** ) and globulin (0.567 ** ), and negatively correlated with α-1 glutelin (-0.612 ** ). Therefore, the results indicated that albumin, globulin, and α-1 glutelin can affect the eating quality of japonica and tongil-type rice varieties, with the latter having lower eating quality than the former.
저장에 따른 생태형별 현미의 발아율과 지질 성분의 변화
곽지은,이점식,윤미라,김인환,이정희,김미정,이춘기,김보경,김욱한,Kwak, Jieun,Lee, Jeom-Sig,Yoon, Mi-Ra,Kim, In-Hwan,Lee, Jeong-Heui,Kim, Mi-Jung,Lee, Choon-Ki,Kim, Bo-Kyeong,Kim, Wook-Han 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.5
토콜즈(tocols) 이성질체 조성이 서로 다른 자포니카와 통일형 품종을 이용하여 저장기간에 따른 지질 관련 품질의 변화를 살펴보았다. 자포니카와 통일형 품종의 현미를 $35^{\circ}C$, 습도 80%에서 8주간 저장하며, 2주 간격으로 품질 변화를 분석한 결과, 자포니카 품종들의 발아율은 저장 초기 98.0%에서 저장 8주 후 22.4%까지 감소한 데 반해, 통일형 품종들은 저장 초기 93.3%에서 8주 후 59.7%로 감소해 통일형 품종의 발아율 감소율이 2배 이상 낮았고, 쌀의 저장 중의 산패 정도를 나타내는 지방산가 또한 통일형 품종에서 유의적으로 낮았다. 발아율 및 지방산가는 쌀의 저장 중 품질 저하를 판단하는 주요 기준이며, 본 실험을 통해 통일형 벼 품종이 자포니카 품종에 비해 저장에 따른 품질 저하 속도가 늦다는 사실을 확인하였다. 통일형 벼는 토코페롤 대비 토코트리에놀 함량이 높고, 특히 지질 안정성 및 지질의 산화 억제 효과가 뛰어난 ${\gamma}$-토코트리에놀의 함량이 높은 지질 특징을 지니고 있어, 벼 저장 중의 지질 산패 지연에 관여하였을 것으로 추측된다. 그러나 벼의 저장성과 관련된 명확한 성분 구명을 위해서는 자포니카와 통일형의 토콜즈(tocols) 조성 혼합물을 이용한 저장 실험이 추가로 수행되어야 할 것으로 생각된다. The objective of this study was to determine quality changes in seed germination rates and lipid components in Japonica and Tongil (indica/japonica) type brown rices with different tocol composition during storage. Moisture content, seed germination rate, crude lipid content, fat acidity, tocol content, free fatty acid composition of brown rice and pH of milled rice were measured to evaluate their quality after storage. Seed germination rates of stored Japonica and Tongil type brown rices were decreased by 22.4% and 59.7%, respectively, after 8 weeks of storage. The fat acidity of Japonica rice was significantly (p<0.05) higher than that of Tongil type brown rices after storage. The major tocol homologue of Tongil type was ${\gamma}-tocotrienol$, whereas, major tocol homologues of Japonica brown rices were ${\alpha}-tocopherol$ and ${\alpha}-tocotrienol$ throughout the entire storage period.
위의 상피 형성이상과 샘암종에서 Galectin-3와 Galectin-7의 발현
곽지은,주미,장선희,심상화,이혜란,김한성 대한병리학회 2008 Journal of Pathology and Translational Medicine Vol.42 No.6
Background : Galectin-3 and galectin-7 may play roles in human carcinogenesis. The aim of this study was to investigate the expressions of galectin-3 and galectin-7 in gastric dysplasia and adenocarcinoma. Methods : We examined the expressions of galectin-3, galectin-7, and P53 in nonneoplastic gastric mucosa, gastric epithelial dysplasia (GED), and adenocarcinoma by immunohistochemistry. Twenty cases of nonneoplastic gastric mucosa, 20 cases of low grade GED, 20 cases of high grade GED, 40 cases of early gastric cancer (EGC), and 60 cases of advanced gastric cancer (AGC) are included for study. Results : In the nonneoplastic tissue, only galectin-3 was expressed in the intestinal metaplasia. Galectin-7 was not stained in the nonneoplastic gastric mucosa. Galectin-3 was expressed in 3 cases of low grade GED (15%), 13 cases of high grade GED (65%), 2 cases of EGC (5%), and 5 cases of AGC (8%), respectively. Galectin-7 was expressed in 2 cases of low grade GED (10%), 3 cases of high grade GED (15%), and 4 cases of EGC (10%), respectively. However, it was not expressed in AGC. Conclusions : Based on our results, the expressions of galectin-3 and galectin-7 may contribute to the development of gastric epithelial dysplasia and early gastric cancer, rather than contributing to the progression of gastric adenocarcinoma.
곽지은,김한성,주미,장선희,심상화,지제근,김욱성 대한병리학회 2007 Journal of Pathology and Translational Medicine Vol.41 No.3
Lipomatous hypertrophy of the interatrial septum is a benign cardiac mass that is characterized by excessive deposition of fat in the interatrial septum. It typically occurs in elderly, obese patients and may cause arrhythmia. We report a case of lipomatous hypertrophy of the interatrial septum. A 45-year-old man was admitted for evaluation of chest discomfort. Transthoracic echocardiography revealed a cardiac mass, which was suspected as a myxoma. A resection of the tumor was performed. Grossly, the mass was 1.3×1.2×0.8 cm, and showed yellow soft consistency and good demarcation. Microscopically, the mass consisted of mature adipose tissue, intermixed cardiac muscle fibers, entrapped nerve fibers and ganglion cells. Lipomatous hypertrophy of the interatrial septum should be differentiated pathologically from tumorous cardiac mass such as lipoma and myxoma.