부산·울산·경남지역 직업병 감시체계

김정일,김병권,김정원,채창호,이철호,강동묵,김지홍,김진하,김영욱,이영하,이지호,김정호,윤형렬,유철인,정백근,장태원,김운규,윤동영,강진욱,김종은,안진홍,이동준,장준호,이광영,송혜란,최영희,이용환,조병만,최홍렬,고상백,김은아,이유진,홍영습,정갑열,김정만,김준연 大韓産業醫學會 2004 대한직업환경의학회지 Vol.16 No.1

Objective: Occupational medicine specialists in the Busan, Ulsan and Kyung-Nam areas established an area-based occupational disease surveillance system and used this system to collected case information for the purpose of preventing occupational diseases Methods: l l hospital participated in this system. The authors selected five main diseases, which were hand-arm vibration syndrome (HAVS), work-related musculoskeletal disorder (WRMSD), occupational asthma, occupational skin disease and occupational and occupational lung cancer and established their case definitions. All cases were reported on the web, and real time analysis was conducted. Results: Between April 2001 and April 2003, 192 cases of HAVS, 118 cases of WRMSD, 33 cases of occupational asthma, 17 cases of occupational skin disease and 17 cases of occupational lung cancer (for a total of 377 cases of these five main diseases) were reported. most of the HAVS cases came from shipbuilding, and 172 of them (92.7%) were associated with grinding. Of the four main types of WRMSD, carpal tunnel syndrome (CTS) was the most prevalent with 46 cases and shipbuilding was also the main industry involved (83.9%). The ergonomic risk factors involved mainly associated with the hand. In 19 (57.6%) and 4 (12.1%) cases of occupational asthma, the agents involved. The causative agents of occupational lung cancer included Cr, welding fume, PAH, Ni, etc. 10(58.8%) of the cases were diagnosed as squamous cell carcinoma and 5(29.4%) as adenoma. Conclusion: This result showed that an area-based occupational disease surveillance system might provide an effective method of evaluating the prevalence of such diseases, however the Busan, Ulsan and Kyung-Nam provinces are too large to be treated as individual areas. Therefore, the authors suggest that each province should establish its own surveillance system.

조혈모세포이식 후 발생한 주폐포자층 폐렴에 대한 고찰

주지현,최정현,이동건,백지연,고윤호,이혜정,김세희,신호진,박윤희,박지영,김유진,신완식,김춘추 대한감염학회 2001 감염 Vol.33 No.4

Background : Pneumocytitis cainii pneumonia (PCP) can occur in immunocompromised hosts especially such as AIDS or cancer patients. Although recent research had focused on PCP in AIDS patients, few studies have described the clinical presentations of PCP in recipients of stem cell transplantation (SCT). We evaluated the clinical manifestations of PCP in SCT patients admitted at St. Mary's hospital, Seoul, Korea. Methods : The medical records of 17 PCP patients undergoing SCT between Feb. 1998 and Feb. 2000 were reviewed. The diagnosis of PCP was confirmed through the demonstration of Pneumocytitis cainii via either cytology of brochoalveolar lavage (BAL) or histological technique of lung biopsy. CMV disease and CMV infection were confirmed by BAL culture and antigenemia respectively . Results : Seventeen patients were all recipients of allogeneic SCT and 7 of 17 patients were performed non-sibling SCT. Patients presented with symptoms including brief period (4 ∼23 days) of fever (76%), dyspnea (70%), cough (64%), and signs such as rare(58.8%), Sixteen patients (94%) had been receiving immunosuppressive agent such as cyclosporine A (64%) or Fk506 (35%) without PCP prophylaxis. Eleven patients (64%) were treated with corticosteroid with mean dose of 16 mg/day prednisolone and mean duration of 4.6 months after post-SCT period. Twelve patients were co-infected with CMV. Another co-infected miCroorganisms were Pseudomonas aeruginosa, Mycobacterium tuberculosis, herpes simplex virus, parainfluenza virus, Average duration of treatment with trimethoprim-sulfamethoxazole (TMP/SMX) was 21 ±9 days. Four patients died, and three of them were related with PCP. Conclusion : PCP developed frequently in patients who were taking immunosuppressive drug due to graft versus host disease or were not taking TMP/SMX prophylaxis. High risk patients showing fever, cough, or dyspnea should be considered to take early bronchoscopic intervention for detection of PCP. When treat for PCP, it also be considered to the possibility of coinfection such as CMV. (Korean J Infect Dis 33:273∼279, 2001)

The LC-MS/MS Based Multi-omics Analysis to Investigate Clostridioides difficile Infection Using in vitro Coculture Device, The Mimetic Intestinal Host-Microbe Interaction Coculture System, MIMICS

Ji-eun KWON,Sung-Huyn JO,Jae-Seung LEE,Hyo-Jin JEON,Ji-Hyeon PARK,Ye-Rim KIM,Min-Gyu KIM,Ji-Hyun BAEK,Yun-Gon KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

Clostridioides difficile is a gram-positive bacterium and is considered as an enteric pathogen. C. difficile colonizes the host with intestinal dysbiosis and produces virulence factors, Toxin A and B. Although the cytotoxicity of C. difficile has been well elucidated, the exact pathogenic mechanisms and biological pathways of C. difficile infection (CDI) are not yet clear. Here, we developed a CDI model using MIMICS to investigate C. difficile-host interactions throughout the early (12 hr) and later (48 hr) stages of infection. We also apply an LC-MS/MS-based multi-omics approach to study the physiological changes in CDI-induced gut epithelial cells at the molecular and cellular levels. The proteomic analysis confirmed that proteins involved in cellular responses such as stress, immune responses and DNA damage responses are upregulated at early stages of infection. In addition, a representative CDI phenotype has been identified in the late stage of the infection. Our in vitro CDI model and multi-omics approach not only provide a better understanding of host-anaerobic pathogen interactions but may also aid in the treatment of C. difficile and other anaerobic infections.

LC-MS/MS Based Multi-omics Approaches for Prebiotic Inulin Utilization on Faecalibacterium prausnitzii

Ji-Hyeon PARK,Sung-Hyun JO,Jae-Seung LEE,Hyo-Jin JEON,Ji-Eun KWON,Ye-Rim KIM,Ji-Hyun BAEK,Min-Gyu KIM,Yun-Gon KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

The human gut commensal bacteria Faecalibacterium prausnitzii is well known for its anti-inflammatory effects that improve host intestinal health. Although several studies reported that inulin, one of the well-known prebiotics, increases the abundance of F. prausnitzii in the intestine, the mechanism under this effect of inulin remains unclear. Here, we applied LC-MS/MS-based multi-omics approaches to confirm the effects of inulin on F. prausnitzii. Interestingly, the proteomic analysis revealed that the putative proteins involved in sucrose utilization of F. prausnitzii are upregulated in the presence of inulin. To investigate the function of the proteins, we cloned the target genes, and observed the ability for sucrose degradation. In addition, we demonstrated that the sucrose degradation activity in F. prausnitzii culture media is enhanced by using inulin as a carbon source compared to glucose. Taken together, this study suggests that the reduction of sucrose induced by inulin could potentially improve host health and prevent gut dysbiosis like type 2 diabetes which is associated with sucrose.

Questionnaire-based Survey of Demographic and Clinical Characteristics, Health Behaviors, and Mental Health of Young Korean Adults with Early-Onset Diabetes

Park Ji In,Baek Hyunjeong,Kim Sang-Wook,Jeong Ji Yun,Song Kee-Ho,Yu Ji Hee,Nam-Goong Il Sung,Cho Eun-Hee 대한의학회 2021 Journal of Korean medical science Vol.36 No.26

Background: The incidence of early-onset diabetes is increasing among young adults. However, there are limited data on the characteristics and management of young Korean adults with diabetes. This study assessed the clinical and demographic characteristics, health behaviors, and mental health among young Korean adults with diabetes mellitus. Methods: This cross-sectional study included young Korean adults with diabetes (n = 225) with an onset age of 20–39 years from four university hospitals. Demographic characteristics, management of diabetes, and mental health were assessed using a questionnaire survey. Results: Type 2 diabetes was the most common type (73.3%), and 13.8% of participants were classified as other types or unknown. Approximately, 64.7% of participants had a strong family history of diabetes, and 76% had treatment within three months of diagnosis. Approximately, 11.1% of participants had diabetic complications; 39.1% of participants received insulin injections, including oral anti-diabetic medications. Additionally, 30.4% were smokers, and only 28% had active physical activity; 26.5% of participants had >3 hours of screen time. One third of participants never had breakfast, and 60.5% went out to eat at least three times a week. Half of the participants showed moderate to severe stress perception, and 21.4% of patients had moderate to severe levels of depression based on the Korean version of Beck Depression Inventory score. Conclusion: Early-onset diabetes was associated with a strong family history and early insulin treatment. Young adults with diabetes had poor health behaviors and frequent mental depression. These findings suggest the necessity of health policies for improving health behaviors and mental distress.

High Glucose Causes Human Cardiac Progenitor Cell Dysfunction by Promoting Mitochondrial Fission: Role of a GLUT1 Blocker

( He Yun Choi ),( Ji Hye Park ),( Woong Bi Jang ),( Seung Taek Ji ),( Seok Yun Jung ),( Da Yeon Kim ),( Songhwa Kang ),( Yeon Ju Kim ),( Jisoo Yun ),( Jae Ho Kim ),( Sang Hong Baek ),( Sang Mo Kwon ) 한국응용약물학회 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.4

Cardiovascular disease is the most common cause of death in diabetic patients. Hyperglycemia is the primary characteristic of diabetes and is associated with many complications. The role of hyperglycemia in the dysfunction of human cardiac progenitor cells that can regenerate damaged cardiac tissue has been investigated, but the exact mechanism underlying this association is not clear. Thus, we examined whether hyperglycemia could regulate mitochondrial dynamics and lead to cardiac progenitor cell dysfunction, and whether blocking glucose uptake could rescue this dysfunction. High glucose in cardiac progenitor cells results in reduced cell viability and decreased expression of cell cycle-related molecules, including CDK2 and cyclin E. A tube formation assay revealed that hyperglycemia led to a significant decrease in the tube-forming ability of cardiac progenitor cells. Fluorescent labeling of cardiac progenitor cell mitochondria revealed that hyperglycemia alters mitochondrial dynamics and increases expression of fission-related proteins, including Fis1 and Drp1. Moreover, we showed that specific blockage of GLUT1 improved cell viability, tube formation, and regulation of mitochondrial dynamics in cardiac progenitor cells. To our knowledge, this study is the first to demonstrate that high glucose leads to cardiac progenitor cell dysfunction through an increase in mitochondrial fission, and that a GLUT1 blocker can rescue cardiac progenitor cell dysfunction and downregulation of mitochondrial fission. Combined therapy with cardiac progenitor cells and a GLUT1 blocker may provide a novel strategy for cardiac progenitor cell therapy in cardiovascular disease patients with diabetes.

국내 가정폭력 연구 동향 분석 : 주요 상담학회지를 중심으로(2011∼2020)

백윤영(Yun-Young BAEK),홍지선(Ji-Seon HONG),김순영(Soon-Young KIM),박성지(Sung-Ji PARK) 한국상담심리교육복지학회 2021 상담심리교육복지 Vol.8 No.3

본 연구는 국내 가정폭력 연구의 동향을 분석하여 향후 가정폭력 연구 방향에 시사점을 제공하는 데 목적이 있다. 이를 위해 KCI 등재학술지들에 2011년부터 2020년 6월까지 게재된 가정폭력 관련 논문 중 “가정폭력”, “학대”, “방임”, “정서학대”, “성 학대”를 주제로 연구된 논문 125편을 대상으로 범주분석과네트워크분석을 실시하였다. 가정폭력 연구는 10년간 평균 12.5편씩 꾸준히 연구되었다. 연구대상은 중학생, 대학생, 고등학생 순으로 이루어졌으며, 남녀 혼합의 양적 연구가 주로 이루어졌다. 양적연구의 주제는 변인 간 관계연구가 89.6%로 거의 대부분을 차지하였다. 가정폭력 유형은 정서적 폭력, 신체적 폭력, 방임 순으로 연구되었으며 대다수의 가정폭력 논문이 가정폭력 피해자 중심의 연구로 진행되었다. 키워드 네트워크 분석은 NetMiner 4.0을 활용하여 중심성과 응집성 분석, 토픽 모델링을 하였다. 본 연구의 결과를 바탕으로 가정폭력 연구의 주요 시사점 및 추후 연구를 위한 제언을 논의하였다. The purpose of this study is to provide implications for the future direction of domestic violence researches by analyzing the trends of domestic violence researches in Korea. For this purpose, among domestic violence studies published in the KCI journal from 2011 to June 2020, 125 studies on the subject of family violence , abuse , neglect , emotional violence , and sexual abuse were analyzed by category analysis and keyword network analysis. Domestic violence researches have been steadily studied an average of 12.5 papers per year for the last 10 years. The subject of the studies consisted of middle school students, college students, followed by high school students, and male and female mixed, quantitative studies were mainly conducted. The theme of quantitative researches was to reveal the relationship between domestic violence and other psychological issues and it occupied 89.6% of the entire portion. The types of domestic violence were studied emotional violence, physical violence, followed by neglect, and the majority of researches was conducted as studies focused on victim. For keyword network analysis centrality and coherence analysis, and topic modeling were performed using NetMiner4.0. The implications of the findings and the suggestions for further research were discussed.

탈북작가의 `몸` 형상화와 윤리적 주체의 가능성 - 김유경의 소설을 중심으로 -

백지윤 ( Baek Ji-yun ) 한국현대문예비평학회 2017 한국문예비평연구 Vol.- No.54

본 연구는 탈북작가 김유경의 장편소설 『청춘연가』와 『인간모독소』를 대상으로 삼아 탈북작가의 자기서사적 형상화 양상을 살폈다. 김유경의 소설은 이전의 탈북작가와는 차별화된 글쓰기 방식으로 주목받으며 문학적 성취를 이루었다는 평가를 받고 있다. 이러한 평가의 배경에는 그의 소설이 탈북자의 현실을 충실히 재현하며 탈북자의 의식을 총체적이고 객관적으로 보여준다는 인식이 놓여 있다. 현실의 재현과 집단성 표출이라는 문학적 인식은 일면 타당하지만 문학과 현실의 관계를 `반영`이라는 일차원적 관계로 한정해 이해한다는 점에서 한계를 노정한다. 본 연구는 이러한 지점을 연구의 출발점으로 삼아 현실을 재현하는 동시에 극복하려는 작가적 욕망의 구체성에 주목하였다. 두 소설이 보여주는 탈북작가의 자기서사화 과정은 폭력적 현실에 저항하며 윤리적 주체되기의 여정과 다름없다. 폭력의 대상에서 윤리적 주체로 이행이 가능한 것은 `몸`이라는 물질성에 주목하기 때문이다. 몸의 물질성은 자연적 소요나 고정적 본질로 주어진 것이 아니다. 물질성은 주체가 끊임없이 변화할 수 있는 운동성 있는 장으로 몸이 존재하도록 만든다. 물질성이 충만한 장인 몸을 통해서 주체는 주거하며 삶을 향유하고 노동하고 소유한다. 또한 몸을 통해 타자와의 만남이라는 사건을 쉼 없이 경험한 주체는 타자에게 자신을 열어젖힘으로써 윤리적 주체로 나아간다. 본고는 두 소설이 그려내는 이러한 주체되기의 여정을 레비나스의 사유에 기대어 충실히 따라가며 작품에 내재된 윤리적 주체의 가능성을 해명하고자 하였다. 이를 통해 작품의 재현양상에 주목한 기존연구에서 한걸음 더 나아가 탈북작가의 글쓰기를 자기서사화라는 관점에서 새롭게 읽어내고자 하였다. This study analyzes the aspects of self-narratives presented in the novels by North Korean defector novelist Kim Yu Kyung. Her novels have received critical acclaim for its distinguished writing and have been praised for its aesthetic achievement comparable to that of South Korean literature at the time. Reason being, her novel realistically reproduced the reality and effectively revealed the holistic consciousness of North Korean defectors from an objective point of view. However, these broad connections limit the perspective on literature as it creates a one-dimensional understanding that literature is simply a reflection of reality. Therefore, this research focuses on revealing the author`s desire to not just simply reflect reality but to overcome it. By analyzing Kim Yu Kyung`s works < Love Song for Youth > and < Place of Human Desecration >, this paper provides evidence that the self-narratives presented in these novels represent how the protagonists not only expose their violent reality but in the process of revealing their circumstances and actions as the objects of aggression, become the ethical subjects of the narrative. The reason for this transcendence is due to the materiality of the body. Although the body is a recipient of pain when violence is acted upon it, it can also become the source of healing due to its materiality. By referencing Emanuel Levinas` ethics of the Other, this paper demonstrates how the subject, through meeting and interacting with others, becomes the ethical subject beginning from the body where the subject first exists.

LPS로 유도된 RAW 264.7 대식세포에서 닭발, 오가피, 두충 혼합 추출물의 항염증 활성

백지윤(Ji Yun Baek),강기성(Ki Sung Kang),이아영(Ah Young Lee),김현영(Hyun Young Kim) 한국생물공학회 2020 KSBB Journal Vol.35 No.4

This study was to investigate the anti-inflammatory effect of chicken-feet, Acathopanax, and Eucommia ulmoides Oliver mixture (CAEM) extract against lipopolysaccharide (LPS) in RAW 264.7 macrophage cells. We examined cytotoxicity of CAEM extract on RAW 264.7 cells, and their anti-inflammatory properties by measuring nitric oxide (NO) and pro-inflammatory cytokine production. In addition, inflammation-related gene expression was analyzed by real time-PCR in LPS-stimulated RAW 264.7 macrophage cells. Treatment of CAEM extract at concentrations of 0.31-2.5 mg/mL did not have any cytotoxic effect in RAW 264.7 cells. Treatment of LPS significantly increased the NO production, whereas CAEM extract (1.25, 2.5mg/mL) significantly decreased the levels of NO, compared to only LPS-treated cells. Furthermore, treatment with CAEM extract significantly suppressed the LPS-induced interleukin (IL)-6 and tumor necrosis factor-α (TNF-α) production. In order to confirm whether CAEM extract regulates inflammation-related gene expression, we analyzed the mRNA levels of IL-6, TNF-α, IL-1β, and inducible NO synthase (iNOS) by real time-PCR. Exposure of RAW 264.7 cells on LPS upregulated the mRNA levels of IL-6, TNF-α, IL-1β, and iNOS, compared to control group. However, those of expression were downregulated by treatment with CAEM extract. These findings suggest that CAEM extract may contribute to the prevention and treatment of inflammatory-related diseases.

신생아에서 수질자궁증과 수신증의 원인이 된 처녀막 폐쇄증

백윤지 ( Yun Ji Baek ),김정태 ( Jeong Tae Kim ),장성규 ( Sung Kyoo Jang ),최욱환 ( Ook Hwan Choi ) 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.2

Imperforate hymen is a common congenital malformation, but usually remains asymptomatic and not detected until menarche. Neonatal hydrometrocolpos caused by imperforate hymen is reported to be very rare. We report a case of hydrometrocolpos in newborn presenting with pelvic mass and bulging membrane in vaginal introitus causing both hydronephrosis which is treated surgically with good result.