Trp P-1 변이원성에 대한 Resveratrol의 항돌연변이 효과

장귀현,안병용,권용주,최동성 한국식품영양학회 2001 韓國食品營養學會誌 Vol.14 No.4

Trp P-1에 의해 유도된 돌연변이원성에 대한 resveratrol의 억제효과를 Salmonella typhimurium TA 98, 100를 이용한 Ames test법으로 검색하였다 먼저 틀변경 돌연변이를 일으키는 S. typhimurium TA98과 염기치환 돌연변이 특성을 지닌 S.typhimurium TA 100에서 Trp P-1의 변이원성을 비교한 결과 S. typhimurium TA98에서 복귀돌연변이의 뚜렷하게 수가 증가되었다. 이러한 결과는 Trp P-1의 돌연변이 유발은 틀변경을 주로 일으킨 돌연변이 유도란 사실을 뒷받침하는 결과로 해석되며,S. typhimurium TA98에서 Trp P-1의 최적농도는 400 ng로 나타났다. S. typhimurium TA98의 생육에 영향을 미치지 않는 농도까지 resveratrol의 농도를 assay당 2, 10, 25, 50 100, 300 ㎍으로 증가시켰을 경우 항돌연변이 효과는 각각 13%, 30%, 52%, 65%, 81% 및 89%로 나타났다. 이러한 결과로부터 Trp P-1의 변이원성에 대하여 resveratrol은 강한 돌연변이 억제효과를 나타내며, 용량 의존적으로 억제함이 확인되었다. The antimutagenic activity of resveratrol on the mutagenicity induced by Trp P-1(3-amino-1,4-dimethyl-5H-pyrido{4,3-b}indole) was studied using the Ames test with Salmonella typhimurium TA98 and 100. Trp P-1 showed strong mutagenecity in S. typhimurium TA98, but was higly decreased mutagenecity in S. typhimurium TA100. This result suggests that the mutagenecity of Trp P-1 can be mainly induced by the DNA lesions causing frame shift. Resveratrol itself did not show antibacterial effect upon 300 rig/assay. Resveratrol showed the strongest inhibitory effect with dose dependent manner on the mutagenicity induced by Trp P-1. The inhibition rates of resveratrol at concentration of 2, 10, 25, 50, 100, 300 ㎍/assay were 13%, 30%, 52%, 65%, 81%, 89%, respectively.

약년 흡연자에서 Body Plethysmograph를 포함한 폐기능 검사 성적에 관한 연구 : 흡연의 급성 및 만성 효과 Acute and Chronic Effect of smoking

이상도,권오정,이춘택,조상헌,남귀현,한성구,심영수,김건열,한용철 대한내과학회 1990 대한내과학회지 Vol.38 No.3

It is well known that cigarette smoking induces chronic obstructive lung disease. Recently it has been noted that inflammatory changes of the small airways are the earliest lesions of chronic obstructive lung disease. Impairment of pulmonary function in teenage smokers, or in nonsmokers who are exposed chronically to cigarette smoke, is reported frequently, and after stopping cigarette smoking, restoration of pulmonary function is observed. Many studies are currently being carried out regarding pulmonary function tests for early detection of small airway disease. Different from chronic effect of cigarette smoking, increased resistance of central airways is reported as an acute effect of cigarette smoking, while others report increased resistance of small airways as an acute effect of cigarette smoking. There is many controversies about the major site of acute effect of cigarette smoking. Maximal expiratory flow volume curve, airway resistance, compliance(static and dynamic) and closing volume was measured in healthy medical students without present symptoms of pulmonary disease, with the object of comparison of the sensitivity of pulmonary function test items used for detection of small airway disease in young-age smokers, and to study about the acute effect of cigarette smoking. The results are as follows; 1) Mean age of smokers was 22.9±1.2(21~26) years and the mean of their smoking history was 4.2±1.2(3~7) pack-year. Mean age of nonsmokrs was 22.0±1. 0(21~23) years. 2) Compared to nonsmokers, CV/VC was significantly high(p<0.05) and C1.0/C0.0(Cdyn at repiration rate of 60/min/Cst) was significantly low(p<0.05) in smokers, while other pulmonary function test items showed no significant difference between smokers and and nosmokers. 3) Of 21 smokers, 12 persons(57%) showed significant decrease of Cdyn with an increase of respiration rate, while 2 persons(13%) showed abnormal MMF and other 2 showed abnormal CV/VC among 16 smokers who performed the tests and they all showed significant decrease of Cdyn with an increase of respiration rate. 4) Of 21 smokers, 13 persons(62%) had symptoms of cough or sputum, but there was no significant difference of pulmonary function tests between symptomatic and asymtomatic smokers. 5) After smoking, FEV1/FVC, MMF and PEFR were decreased significantly(p<0.05), and SRAW was increased significantly(p<0.005). 6) After smoking, the decrease of C1.0/C0.0 was significantly larger(p<0.05) in nonsmokers compared to smokers and increase of SRAW was somewhat larger in nonsmokers but without statistical significance.

Sarcomatoid carcinoma of the mandible: report of a case

Gui-Young Kwon,Young-Jun Choi,Min-Seok Song,Kyoung-In Yun 대한구강악안면외과학회 2010 대한구강악안면외과학회지 Vol.36 No.3

Sarcomatoid carcinoma is a rare and occurs mainly in the upper aerodigestive tract such as the oral cavity, esophagus and vocal cords. It is a unique variant of squamous cell carcinoma. We report the case of a patient with spindle cell squamous cell carcinoma involving the mandible. At initial examination, overlying mucosa of that lesion was normal appearance. One week later, that lesion showed ulcerative and bloody change and rapid growth in size. This case showed unpredictable rapid growth although rapid growth in size was suspected of undergoing malignancy.

Mast Cell and Macrophage Counts and Microvessel Density in Invasive Breast Carcinoma-Comparison Analysis with Clinicopathological Parameters

Gui Young Kwon,Sang Dae Lee,박언섭 대한암학회 2005 Cancer Research and Treatment Vol.37 No.2

Purpose: The purpose of this study was to evaluate the clinicopathological significance of the microvessel density and macrophage and mast cell counts in invasive breast carcinomas.Materials and Methods: 45 invasive breast carcinomas were immunohistochemically stained with the endothelial antigen, CD34, and macrophage marker, CD68. 0.1% toluidine blue was used to highlight mast cells. The microvessel and mast cell counts were performed at ×200 magnification and the macrophages at ×400 magnification.Results: With the 45 invasive breast carcinomas, there were no statistically significant associations between the mast cell, macrophage and microvessel counts and the tumor size and lymph node status. ER and PR negative mast cells infiltrated more than in cases of positive stati,with statistical significance (p-value=0.010 and 0.005,respectively). The macrophage counts were negatively correlated with the PR status (p-value=0.030). With respect to the c-erbB-2 status, there was no significance correlation with the mast cell, macrophage and microves-selcounts. The mast cell counts showed significantly positive correlation with the microvessel counts in the invasive breast carcinomas (p-value=0.015). In a comparisonof the macrophage counts with the microvessel counts, a positive tendency for both parameters, but without statistical significance (p-value=0.310).Conclusion: Increasing numbers of mast cells and macrophages were recruited in invasive breast carcinomas, which contribute to angiogenesis. The microvesseldensity in invasive breast carcinomas had no statistically significant association with the tumor size, lymph node status, and histological grade, presence of DCIS component, estrogen/progesterone receptor status and cerbB-2 status. The evaluation of angiogenesis using these methods is not thought to provide an independent clinicopathological factor in invasive breast carcinomas.

임신 중 진단된 재생불량성 빈혈의 임상적 특징 및 예후

권지영 ( Ji Young Kwon ),조윤성 ( Youn Sung Jo ),안현영,이귀세라 ( Gui Se Ra Lee ),이영 ( Young Lee ),신종철 ( Jong Chul Shin ),이종건 ( Jong Kun Lee ),나종구 ( Jong Gu Rha ),김수평 ( Soo Pyung Kim ) 대한주산의학회 2006 大韓周産醫學會雜誌 Vol.17 No.2

목적 : 임신 중 발생한 재생불량성 빈혈의 임상적 특정 및 임신과 신생아에 미치는 예후를 알아보고자 본 연구를 시행하였다. 방법 : 1996년 1월부터 2005년 6월까지 본원에서 분만한 산모 중 임신 중 재생불량성 빈혈을 처음으로 진단받은 산모 14명을 대상으로 임상적 특정 및 분만기록과 신생아기록을 조사하였다. 결과 : 14명 중 11명(78%)에서 임신 제2 삼분기 이후에 재생불량성 빈혈을 진단받았다. 발견 당시부터 중증의 재생불량성 빈혈이었던 경우 3명을 포함하여 총 8명이 임신 기간 중 중증의 재생불량성 빈혈이었다. 모든 경우에서 임신 중 약물요법을 시행하지 않았다. 산전 진찰기간 중 수혈을 받은 경우는 7명 이었고, 분만 전후에 수혈을 받은 경우는 13명이었다. 분만 후 추적 관찰이 가능했던 12명에서 1명이 분만 후 완전관해가 되었으며 8명은 부분관해가 되었다. 2명은 골수이식 후 완전관해가 되었다. 조산이 1예가 있었으며 1예에서는 신생아 빈혈이 있었다. 결론 : 임신 중 재생불량성 빈혈의 예후는 기존의 보고에 비해서 매우 향상되었으며 혈색소와 혈소판 수혈을 통한 보존적인 치료가 매우 중요하다. Objective: Pregnancy-associated aplastic anemia remains a rare occurrence. The aim of this study was to examine the maternal and fetal outcomes of pregnancy-associated aplastic anemia treated with supportive care. Methods: From January 1995 to December 2004, a total of 14 women newly diagnosed with pregnancy-associated aplastic anemia were recruited for the study. Results : Eleven (78%) of the 14 women were diagnosed with pregnancy-associated aplastic anemia during the second or third trimester. There were eight severe cases; three of which were diagnosed at the initial presentation. All 14 women had conservative management with transfusions but not specific immunological or hormonal therapies during pregnancy. Blood transfusions were performed prenatally in seven mothers and perinatally in 13. Of the 12 patients eligible for follow-up, one achieved complete remission and another eight showed partial remission after delivery. During the follow up period, there was no case of maternal-fetal death in our series. The pregnancies were continued uneventfully in most cases. Conclusions : This study demonstrated favorable maternal and neonatal outcomes with transfusion support alone for pregnancy-associated aplastic anemia. Therefore, pregnancy continuation with meticulous blood support should be considered, rather than therapeutic termination, for women with pregnancy-associated aplastic anemia.

만삭 임신에서 양수 내 태변착색의 분만 예후

권지영 ( Ji Young Kwon ),신재은 ( Jae Eun Shin ),안세영 ( Se Young Ahn ),이귀세라 ( Gui Se Ra Lee ),김사진 ( Sa Jin Kim ),신종철 ( Jong Chul Shin ),박인양 ( In Yang Park ) 대한주산의학회 2008 Perinatology Vol.19 No.3

목적: 양수 내 태변착색이 발생한 만삭 임신과 태변착색이 없는 임신 간에 분만 전 후의 주산기 예후를 비교하여 이를 바탕으로 태변착색을 동반한 임신의 분만 관리에 도움이 되고자 본 연구를 진행하였다. 방법: 1년 동안 있었던 687 건의 만삭 분만을 양수 내 태변착색이 있었던 89건과 태변착색이 없었던 598명의 두 군으로 분류하였으며 각 군의 분만 및 신생아의 특성을 비교하였다. 산모 연령과 임신주수, 신생아 체중 및 분만방법을 조사하였고 진통 중 안심할 수 없는 태아 심박동 상태의 발생여부, 1분 및 5분 아프가 점수를 살펴보았다. 제대 동맥혈 가스분석을 시행한 577건에서는 제대 동맥혈의 pH, 이산화탄소 분압, 염기결핍의 정도를 조사하였다. 결과: 양수내 태변착색이 있는 군은 태변착색이 없는 군에 비해 진통 중 안심할 수 없는 태아 심박동 상태의 발생이 더 많았고(4.5% vs. 1.0%, p=0.03) 제대 동맥혈 평균 pH값이 7.28±0.13으로 태변착색이 없는 임신에 비해 유의하게 낮았다(p=0.008). 중증의 태아 산증을 의미하는 제대 동맥혈 pH가 7.0 미만인 경우도 총 3.4% 발생하여 태변착색이 없는 경우에 비해 유의하게 많았다(p=0.01). 그러나 대사성 산증의 위험도는 높지 않았으며 분만 후 1분, 5분 아프가 점수도 두 군에서 유의한 차이가 없었다. 결론: 태변착색 임신의 발생율에 비해 분만 후 신생아 상태가 불량한 경우는 많지 않았다. 그러나 발생 가능한 태아 심박동 이상과 태아 산증에 대비해서 분만 중 지속적인 심박동 감시 및 분만 후 주의 깊은 신생아 평가가 필요하다. Purpose: The purpose of this study was to compare the obstetric and perinatal outcome between two groups with or without meconium staining of amniotic fluid (MSAF) at term birth in one-year consecutive population at our delivery unit. Methods: Pregnancy complication including nonreassuring fetal heart rate pattern, intrauterine growth retardation, oligohydramnios, hydramnios and preeclampsia between the two groups were retrospectively documented by review of medical record. To evaluate the perinatal outcome, apgar score at 1min and 5min, and umbilical artery blood gas analysis were also analyzed. Student T test and chi square test were used for statistic analysis. Results: The study population consisted of 687 full-term neonates with presence (n=89) and absence (n=598) of MSAF. Gestational age at delivery was significantly higher in the MSAF group (p<0.001). Although the fetal acidemia (umbilical artery blood pH <7.0) was significantly higher in the MSAF group (3.4% vs 1.0%, p<0.01), the metabolic acidemia (base deficit>12.0 mEq/L) was not increased. The incidence of non reassuring fetal heart rate pattern was also increased in the MSAF group (4.5% vs 1.0%, p=0.03). However there was no significant difference in 1 min and 5 min Apgar score. Conclusion: Although MSAF is associated with the risk of non reassuring fetal heart rate pattern and fetal acidemia, the metabolic acidemia and low apgar score at 5minutes was not significantly increased compared with clear amniotic fluid group. Delivery of pregnancy with MSAF should be managed under the careful fetal heart rate monitoring.

Rapid <i>In Vitro</i> Corrosion Induced by Crack-Like Pathway in Biodegradable Mg-10% Ca Alloy

Jung, Jae-Young,Kwon, Sang-Jun,Han, Hyung-Seop,Yang, Gui Fu,Lee, Ji-Young,Yang, Seok-Jo,Cho, Sung-Youn,Cha, Pil-Ryung,Kim, Young-Yul,Kim, Yu-Chan,Seok, Hyun-Kwang,Ahn, Jae-Pyoung Cambridge University Press 2013 Microscopy and Microanalysis Vol.19 No.5

<B>Abstract</B><P>The <I>in vitro</I> corrosion mechanism of the biodegradable cast Mg-10% Ca binary alloy in Hanks' solution was evaluated through transmission electron microscopy observations. The corrosion behavior depends strongly on the microstructural peculiarity of Mg2Ca phase surrounding the island-like primary Mg phase and the fast corrosion induced by the interdiffusion of O and Ca via the Mg2Ca phase of lamellar structure. At the corrosion front, we found that a nanosized crack-like pathway was formed along the interface between the Mg2Ca phase and the primary Mg phase. Through the crack-like pathway, O and Ca are atomically exchanged each other and then the corroded Mg2Ca phase was transformed to Mg oxides. The <I>in vitro</I> corrosion by the exchange of Ca and O at the nanosized pathway led to the rapid bulk corrosion in the Mg-Ca alloys.</P>

Recent Trends in the Revision of CODEX Nutrient Reference Values

Young Suk Ahn,Ji Eun Park,Eun Jung Lim,Han I Jang,Ye jin Choi,Saet Byeol Jung,Su Ok Kim,Gui Im Moon,Jong Wook Kim,Woo Young Lee,Baeg Won Kang,Hye Kyung Park,Oran Kwon 한국식품영양과학회 2011 한국식품영양과학회 학술대회발표집 Vol.2011 No.10

Recognition and Usage of Nutrition Labelling for Processed Food and Restaurant Meal according to Patterns of Healthy Dietary Behavior in Elementary School Girls

Gui-im Moon,Su-ok Kim,Kwang-il Kwon,Jee-young Yeon,Eun-jung Shin,Hae-na Kim,Ki-yong Shin,Jung-eun Kong,Ha-eun Kim,Hye-kyung Park,Baeg-won Kang 한국식품영양과학회 2012 한국식품영양과학회 학술대회발표집 Vol.2012 No.10

Comparative Effects of Ionizing Radiation Sources on the Quality of Saengshik Powder Products

Gui-Ran Kim,Mi-Seon Jeong,Deokjo Jo,Eun-Joo Park,Ji-Young Kwak,Jae-Jun Ahn,Kashif Akram,Yu-Ri Kim,Bum-Soo Han,Joong-Ho Kwon 한국식품영양과학회 2012 한국식품영양과학회 학술대회발표집 Vol.2012 No.10