당뇨병 환자에서 발생한 대장 가성폐색증 1예

이재형,강준구,박준성,이창범,박용수,김동선,함준수,김태화,안유헌,박충기 대한당뇨병학회 2002 임상당뇨병 Vol.3 No.2

가성 장폐색은 기계적인 폐색부위가 없이 복부팽만과 구토 등 장폐색의 증상과 징후가 나타나는 질환을 말하며, 선행원인을 찾을 수 없는 원발성 가성 장폐색과 다른 질환에 이차적으로 발생하는 속발성 가성 장폐색으로 나눌 수 있다. 당뇨병은 잘 알려진 속발성 가성 장폐색의 원인이지만 실제로 당뇨병에 의한 가상 장폐색의 발생 빈도는 매우 드문 것으로 보이며, 가성 장폐색의 적절한 치료에 대해서는 아직 논란이 있다. 저자들은 2형 당뇨병 환자에게 대장에 국한된 급성 가성 장폐색을 진단하고 보존적 치료를 시행하여 호전된 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Colonic pseudo-obstruction is a disorder characterized by gross dilatation of the colon in the absence of any anatomic lesions, which obstructs the flow of the intestinal contents. Diabetes is a well known cause of secondary pseudo-obstruction but its prevalence in rare and not well understood. A 59-year-old woman patient was admitted due to abdominal distension and nausea of a day's duration. She was diagnosed with diabetes 15 years ago, but the condition had been poorly controoled. On the abdominal radiographs, there were marked small and large bowel dilatation with the, exception of the descending colon. Our initial diagnosis was mechanical obstruction, but the possible causes were not found. Also, we could not find another cause of the secondary pseudo-obstruction, with the exception of diabetes mellitus. Thus, she was diagnosed with a diabetes induced intestinal pseudo-obstruction. The colonic dilatation was resolved by conservative management and she became comfortable in 10 hospital days, and was subsequently discharged.

Protein kinase A-induced phosphorylation at the Thr154 affects stability of DJ-1

Ko, Yeon Uk,Kim, Su-Jeong,Lee, Juhyung,Song, Min-Young,Park, Kang-Sik,Park, Jun Bae,Cho, Hyun-Soo,Oh, Young J. Elsevier 2019 Parkinsonism & related disorders Vol.66 No.-

<P><B>Abstract</B></P> <P><B>Introduction</B></P> <P>Most cases of Parkinson's disease (PD) are sporadic, but genetic variations have been discovered in PD patients. PARK7/DJ-1 is a known cause of early-onset autosomal-recessive PD and is implicated in neuroprotection against oxidative stress. Although several post-translational modifications of DJ-1 have been proposed, phospho-modification of DJ-1 and its functional consequences have been less studied.</P> <P><B>Methods</B></P> <P>Putative phosphorylation sites of DJ-1 were determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS analysis). Subsequently, phosphorylation site of DJ-1 was confirmed by <I>in vitro</I> kinase assay and cell-based pull-down assay. Impaired dimer formation of phospho-null mutant was measured using DSS crosslinking assay and immunoprecipitation assay. To evaluate physiological consequences of this event, protein stability of DJ-1 WT and DJ-1 phospho-null mutant were compared using cycloheximide chase assay and ubiquitination assay.</P> <P><B>Results</B></P> <P>Here, we showed that DJ-1 directly bound to the catalytic subunit of protein kinase A (PKAcα). We found that PKAcα is responsible for phosphorylation of DJ-1 at the T154 residue. Interestingly, dimerization of DJ-1 was not detected in a DJ-1 T154A mutant. Furthermore, stability of the DJ-1 T154A mutant was dramatically reduced compared with that of wild-type DJ-1. We found that DJ-1 T154A was prone to degradation by the ubiquitin proteasome system (UPS).</P> <P><B>Conclusion</B></P> <P>We identified a novel phosphorylation site of DJ-1. Furthermore, we determined protein kinase A that is responsible for this posttranslational modification. Finally, we demonstrated physiological consequences of this event focusing on dimerization and protein stability of DJ-1.</P> <P><B>Highlights</B></P> <P> <UL> <LI> PKAcα phosphorylates PARK7/DJ1 at the Thr154 residue. </LI> <LI> Disruption of Thr154 phosphorylation is linked to a lower stability. </LI> </UL> </P>

관동맥질환에서 저밀도 지단백 입자 크기의 분포

임효영,김인숙,승기배,강동헌,전두수,장기육,박준철,이수영,홍순조,최규보 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.8

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

Al_2O_3를 함유한 Poly(ε-caprolactone)/Poly(ethylene oxide) 마이크로캡슐의 향오일 방출거동

박수진,양영준,이재락,서동학 한국공업화학회 2003 공업화학 Vol.14 No.3

본 실험에서는 소수성의 PCL과 친수성의 PEO의 함량비를 변화시키면서 액증건조법을 이용하여 향오일이 흡착된 Al_2O_3를 심물질로 함유하는 마이크로캡슐을 제조하였다. 또한, Al_2O_3의 화학적 처리가 마이크로캡슐의 향오일 방출거동에 미치는 영향을 고찰해 보았으며, 산-염기 표면처리 용액으로서 30 wt% HCL(Al_2O_3 -HCl)과 30 wt% NaOH(Al_2O_3 -NaOH) 용액을 각각 사용하였다. 처리 전후의 Al_2O_3의 표면 산-염기도는 Boehm 선택 중화법을 이용하여 측정하였다. PEO의 함량에 따른 마이크로캡슐의 형태는 image analyzer와 scanning electron microscope (SEM)을 통하여 관찰하였다. FT-IR 측정을 통하여 심물질이 함입되었음을 확인하였으며, 또한 접촉각 측정을 통하여 표면 자유에너지의 변화를 알아보았다. 그 결과, PCL/PEO 마이크로캡슐의 직경, 표면 자유에너지 그리고 향오일의 방출속도는 PEO의 함량이 증가할수록 증가하였다. 또한 Al_2O_3-HCL을 함유한 PCL 마이크로캡슐의 향오일 방출속도는 감소하였는데, 이는 PCL/PEO 마이크로캡슐의 친수성의 증가와 산-염기 반응에 의해 물리화학적으로 Al_2O_3와 향오일간의 분자간 인력이 증가하였기 때문이라 사료된다. In this work biodegradable polymers of hydrophobic poly(ε-caprolactone) (PCL) and hydrophilic poly(ethylene oxide) (PEO) were prepared in different weight ratios, 100 : 0, 90 : 10, 80 : 20, and 70 : 30, to manufacture the microcapsules containing Al_2O_3 and fragrant oil by an oil-in-water (o/w) emulsion solvent evaporation method. The release behaviors of microcapsules were studied by chemical treatments of Al_2O_3 with 30 wt% HCl and Al_2O_3 with 30 wt% NaOH. The acid and base values were investigated with the Boehm's titration technique. The effect of PEO content on the diameter and shape of microcapsules were observed with image analyzer and scanning electron microscope (SEM). PCL/PEO microcapsules containing flagrant oil were confirmed by FT~IR. The variation of surface free energy of the microcapsules characterized by contact angles was examined in terms of PEO ratio in the blending system. As a result, it was found that the diameter, surface free energy, and fragrant oil release rate of PCL/PEO microcapsules increased with increasing the PEO ratio. However, the fragrant oil release rate of PCL microcapsules containing Al_2O_3-HCl decreased. These results were mainly due to the increased hydrophilicity of the microcapsules or the acid-base interfacial interactions between Al_2O_3-HCL and fragrant oil.

歷史敎育을 통한 國際理解增進에 관한 硏究

朴宗烈,朴埈洙 春川敎育大學 1989 論文集 Vol.29 No.-

간 이식 환자에서 발생한 파종성 Scedosporium apiospermum 감염 1예

박현구,최성호,강호석,송주형,정지훈,소민욱,최상호,김양수,우준희 대한감염학회 2006 감염과 화학요법 Vol.38 No.5

저자들은 다발성 뇌 농양을 동반한 치명적인 파종성 S. apiospermum 감염이 발생한 간 이식 환자를 경험하였다. 본 증례의 환자는 voriconazole로 치료하는 호전경과를 보이다가 진균성 뇌 동맥류 파열로 사망하였다. S. apio-spermum은 비교적 드문 진균으로 병리학적으로 Aspergillus spp.와 매우 유사하고 amphotericin B에 내성을 나타내므로 진단과 치료가 적절하게 이루어지지 못할 가능성이 많고 면역 기능 저하 환자에서 혈행성 전파를 통한 파종성 감염이 용이하게 발생하므로 높은 사망률을 나타낸다. 이식 환자를 비롯한 면역 기능 저하 환자에서 진균감염이 의심될 때 S. apiospermum과 같은 드문 진균에 의한 감염증도 감별진단에 포함해야 하겠다. Sedosporium apiospermum is a saprophytic fungus commonly found in soil and polluted water. This organism is known as a cause of mycetoma, which may occur in immunocompetent hosts following trauma. However, in immunocompromised patients, S. apiospermum can also cause life-threatening invasive disease, including central nervous system infection or disseminated infection. We report a fatal case of disseminated S. apiospermum infection in a 46-year-old woman after liver transplantation. Eight days postoperatively, she developed pneumonia, followed by altered mentality in the 15 days. A head CT demonstrated multiple brain abscesses. Sputum and stereotactic-aspirated brain abscess culture yielded S. apiospermum. Despite treatment with voriconazole, the patient died of intracranial hemorrhage.

다발성 비장경색을 동반한 장티프스균(Salmonella enterica serovar Typhi)에 의한 심내막염 1예

박경민,김남중,오동렬,박진태,김양수,우준희,류지소 대한내과학회 2003 대한내과학회지 Vol.65 No.6

저자들은 3㎜의 우종과 함께 이판의 대동맥판막을 가진 환자에서 다발성 비장경색을 동반한 장티프스균에 의한 감염성 심내막염으로 진단한 환자 1예를 경험하였기에 문헌고찰과 함께 보고한다. Infective endocarditis is a very rare cardiac manifestation of salmonella infection, and splenic infraction is a rare noncardiac complication. We describe a case of Salmonella enterica serpvar Typhi bacteremia which was complicated by infective endocarditis with multiple splenic infractions in a previously healthy 47-year-old female. She didn't have any history of foreign travel. The isolate of Salmonella enterica serovar Typhi was susceptible to cephalosporins, aminoglycosides, quinolones but resistant to ampicillin. After 3 weeks of intravenous and oral therapy with ciprofloxacin, follow up transthoracic and transesophageal echocardiography showed no vegetation. In addition, follow up abdominal CT showed decreased size of splenic infractions. The patient was treated with 2 weeks of intravenous and 4 weeks of oral ciprofloxacin, and was cured without sequelae or relapse for 6 months follow-up.

Baculovirus Vector System에 의해 발현된 재조합 Pseudorabies Virus Major Capsid Protein의 면역원성

전무형,안동준,장경수,조용성,박종현,송재영,현방훈,안수환 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-

The recombinant pseudorabies virus major capsid protein (rMCP) was produced by expression of the MCP gene in Sf-9 cell using baculovirus transfer vector system. Following evaluation of the immunochemical properties of the rMCP, the immunogenicity of the recombinant subunit protiens were investigated in guinea pig and swine to obtain the preliminary guide line for the subunit vaccine using rMCP and gP50. It was proved that ultrasonication and 30% ammonium sulfate was most efficient to concentrate and purify the protein. The rMCP was safe in mice, guinea pigs and piglets. In guinea pigs, rMCP mixed with various adjuvants induced substantial degree of serum neutralizing antibody titers, but revealed incomplete protecivity against challenge. In swine, the combination of rMCP and gP50 showed the higher serum neutralizing antibody titers and cellular immune responses than rMCP alone. However, the protectivity was lower in comparison with the commercial gI-deleted inactivated vaccine. We expect these results to contribute to characterization of MCP gene of Korean isolate of PRV and to ultilize as preliminary information for prodution and evaluation of PRV recombinant subunit vaccines.

ImmunoDot법에 의한 전신성 류마티스 질환의 자가항체 검사

박성환,김선준,김성일,민준기,이상헌,홍연식,조철수,김호연 최신의학사 1997 最新醫學 Vol.40 No.7