목재부후성 담자균류 배양균사체의 생물활성 연구 : Antimicrobial,Plant Growth Regletory,Antitomor and enzymatic activities 향균활성, 식물생장조절활성, 항암활성, 효소활성탐색

조수묵,유승헌,신관철 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-

목재부후성 담자균류의 배양 균사체의 생물활성을 조사하기 위하여 항균활성, 식물생장조절활성, 항암활성 및 그들이 생산하는 효소활성을 검정한 결과 Coriolus versicolor 5129와 C. pubescens 5131 균주는 그람양성세균에, Lenzites betulina 8029 균주는 그람음성세균에 저지 효과를 나타내었으며 L. betulina 8085 균주는 세균과 진균에 저지 효과를 나타내어 광범위한 항균 spectrum을 보였다. 대부분의 균주가 0.8㎖/㎖ 농도 처리시 배추 및 무의 발아 및 생장을 억제하였으며, 특히 Fomitopsis pinicolor 8059와 Fomitella fraxinea 8084 균주의 경우 생육을 강하게 억제하였다. 그러나, Bjerkandera adusta 8054 균주는 0.4 및 0.2 ㎖/㎖ 농도에서 배추 및 무의 생육을 촉진하였다. 몇종의 목재부후균 다당체를 추출하여 암세포를 이식한 ICR mouse에 투여한 결과 거의 전 균주가 항종양 작용을 나타내었으나 버섯종류 간에 큰 차이를 보였고 그중 L. betulina 8029와 미분류된 8058 균주는 효과가 비교적 높았다. 목재부후균의 세룰로스와 리그닌 분해력은 거의 전 공시균주에서 나타났고 종류간에 역가가 다양하여 강력한 분해균의 선발 가능성을 보였다. For screening of biological activities of culture broth of some wood rotting basidiomycetes, antimicrobial activity, plant growth regulating activity, antitumor activity, and various enzyme activities were checked. Coriolus versicolor 5129 and C. pubescens 5131 strains showed inhibition activity against gram-positive bacteria and Lenzites betulina 8029 strain showed the activity against gram-negative bacteria. L. betulina 8085 inhibited the growth of both bacteria and plant pathogenic fungi. All of tested basidiomycetes inhibited the germination and growth of radish and cabbage at concentration of 0.8 ㎖/㎖. Especially, Fomitopsis pinicolor 8059 and Fomitella fraxinea 8084 showed strong inhibition activity. In contrast, Bjerkandera adusta 8054 stimulated the growth of cabbage and radish at concentration of 0.4 and 0.2 ㎖/㎖. All polysaccharides from tested basidiomycetes showed anti-tumor activity against sarcoma 180 and the stronger antitumor activity was observed in L. betulina 8029 and unidentified 8058 strain. All tested basidiomycetes had also an ability to degrade cellulose and lignin.

게놈 유형이 다른 무릇(Scilla scilloides comlpex)에서 FISH를 이용한 인 우성 다양성 분석

이상은,최혜운,방재욱 충남대학교 생물공학연구소 2004 생물공학연구지 Vol.10 No.2

Nucleolar dominance, an epigenetic phenomenon in which one parental set of ribosomal RNA gene is silenced in an interspecific hybrid, was investigated in different cytotype plants of Scilla scilloides complex; AA, BB, AABB, ABBB, BBBB and AABBB. To determine the loci of the nucleolar organizer regions (NORs) in metaphase chromosomes, physical mapping of 45S rRNA gene (3.8 Kb) using FISH method was applied. Two signals of the 45S rRNA gene loci were observed in both the cytotypes of AA (2n=16) and BB (2n=18). Four signals were found in tetraploid BBBB, while two and three signals were found in cytotypes of AABB and ABBB, respectively. However, AABB plants in the population of Daegukhul-do Island had four NORs in both the A and B genome. Four signals were found in pentaploid AABBB plants. The different numbers of 45S rRNA gene signals suggested the phenomenon of nucleolar dominance or amphiplasty among different cytotype plants of S. scilloides. It is speculated that inactivation of NORs on a2 chromosome in the cytotype AABB plants is the result of the amphiplastic suppression between A and B genomes.

바이러스 복제기작에서 Protein Arginine Methyltransferases 의 기능

유정은,신봉진,박의순,최승아,노재랑 충남대학교 생물공학연구소 2009 생물공학연구지 Vol.15 No.-

Protein arginine methylation is one of common post-translational modification and is involved in the modulation of diverse intracellular events including cell signaling, activation and differentiation. Protein arginine methylation is catalyzed by a family of methyltransferase enzymes called the protein arginine methyltransferases (PRMTs). The methylation of arginines is basically catalyzed via S-adenosyl-L-methionine as the methyl donor by PRMTs. Protein arginine methylation is implicated in many RNA metabolism including transcription, RNA splicing and transport, and RNA stability. Interestingly, it has recently come to be appreciated that viral replication is greatly influenced by the activity of cellular PRMT. Viral replication is positively or negatively regulated by the activity of cellular PRMTs through modulation of viral RNA metabolism by protein arginine methylation. For example, the utilization of cellular PRMTl in viral replication of Herpes simplex virus (HSV) has been reported, while viral replication of Human immunodeficiency virus (HIV) is inhibited through arginine methylation of Tat by PRMT6 under hypermethylation conditions. Although there appears to be a relationship between protein arginine methylation and viral replication, the direct involvement of PRMTs in viral replication is still unclear. In this regard, we would like to review current progress and perspectives for the modulation of protein arginine methylation from a point of view in viral replication.

NF-ĸB 신호전달 활성을 통한 Hepatitis C virus core 의 세포 자연사 억제 기작에 대한 연구

노재랑,임동수 충남대학교 생물공학연구소 2004 생물공학연구지 Vol.10 No.1

Hepatitis C virus (HCV) infection is one of major agents for liver diseases including chronic hepatitis, liver cirrhosis and hepatocellular carcinoma in human. Several studies have documented the important association between HCV infection and hepatocellular carcinoma, however, the mechanisms involved are still unknown. Apoptosis plays a critical role to maintain cellular homeostasis both in physiological and pathological conditions. Many viruses encode regulatory or structural proteins, which regulate apoptosis mechanism to escape from host defense mechanism. In particular, the HCV core protein can modulate apoptosis by both proapoptotic or antiapoptotic action, whereas both the NS3 and the NS5A proteins have antiapoptotic effects. In this study, we examined the role of HCV core protein whether it can activate NF-κB signal leading to antiapoptotic process or not. These studies suggest that HCV core protein activates NF-κB signals through increasing the amount of functional NF-κB homodimers or heterodimers in vivo. To further examine the role of HCV core protein in apoptosis process, the full-length of HCV core proteins were expressed and cells were assayed for apoptotic response. The inhibitory effect of apoptosis by HCV core protein may result in HCV persistence and development of hepatocellular carcinoma.

세포사멸 억제단백질인 survivin의 조절과 기능

김미선,김영상,강광일 충남대학교 생물공학연구소 2002 생물공학연구지 Vol.8 No.1

Despite its genetic complexity, the control of cell proliferation and the regulation of cell death are thought to be intimately linked. Survivin, one of the LAP(inhibitor of apoptosis protein) family, is a recently described molecule that has been implicated in both processes, and is overexpressed in most human cancers. Among molecules of the LAP family, survivin is unique in having a single BIR (baculovirus LAP repeat) interacting directly apoptosis executors (e.g.,caspase 3 and caspase 7) as well as in showing cell-cycle related expression that upregulates in G2/M phase. The exploitation of the survivin signaling pathway might provide significant crossroads and important clues between the proliferation and the apoptosis.

Follicle-stimulating hormone transiently induces expression of protooncogene c-myc in primary Sertoil cell cultures of early pubertal and prepubertal rat

Lim, Kyu,Hwang, Byung-Doo 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-

The protooncogene c-myc plays an important role in the regulation of cellular proliferation and differentiation. To evaluate the possibility that the protooncogene c-myc plays some roles in follicle-stimulating hormone (FSH)-dependent gene regulation of Sertoli cells, the effects of FSH on the expression of c-myc has been investigated in primary Sertoli cell cultures. FSH was no change to the c-myc mRNA level before 18 h, but transiently increased c-myc mRNA levels, with maximal stimulation reached in 18 h. The induction of c-myc mRNA was dependent on the concentration of FSH. The c-myc mRNA was also increased after treatment with dibutyryl c-AMP and forskolin in primary Sertoli cell cultures. FSH-dependent c-myc mRNA levels were superinduced in cells treated for 3 h with cycloheximide but it was reduced by actinomycin-D pretreatment. Even in the absence of FSH in culture medium c-myc mRNA was clearly detectable in Sertoli cells from 8-day-old rats but hardly detectable in cells from 14 and 28 days of age. FSH stimulated c-myc mRNA expression in the primary Sertoli cells derived from only 8- and 14-day-old rats but had almost no effect in the 28-day-old rats. These results suggest that FSH induces c-myc mRNA levels in the primary Sertoli cells from prepubertal and early pubertal rats, and then transient expression of c-myc may be responsible for some roles in the regulation of FSH-dependent genes in Sertoli cells.

Cloning of Involution-induced cDNAs from Mouse Mammary Gland

Choi, Bonghwan,Myung, Kyuho,Lee, Mijoung,Kim, Hyungha,Jeon, Deahyun,Hwang, Intaek,Choi, Yunjaie,Paik, Sanggi,Baik, Myunggi 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-

Involution-induced cDNAs from mouse mammary gland were cloned and characterized. After the primary differential screening of a total 40,000 pfu of cDNA library, 200 positive plaques were isolated. Positive plaques were rescreened by PCR/Southern differential nybridization. Two clones were characterized using Northern analysis and nucleotide sequencing. Partial sequences revealed that one clone coded for sulfated glycoprotein-2 (SGP-2). The other clone coded for WDNM1, a member of a family of protease inhibitors The expression of the SGP-2 gene was induced at involution days 1, 2, and 3. The SGP-2 was highly expressed in female mammary glands and testis. The expression levels of SGP-2 were low in thymus, uterus, ovary, liver, brain, and male mammary glands. The WDNM1 gene was induced at an early involution stage (6 h). The high levels of WDNM1 mRNA were maintained until involution day 4. The WDNM1 gene was expressed only in the mammary gland of female mice.

Alcaligenes sp. K-912에서의 poly(3-hydroxybutyrate-co-3-hydroxyvalerate) 생합성에 미치는 아미노산의 첨가 효과

윤주석,윤기영,김정윤,이영하 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-

Alcaligenes sp. K-912의 회분배양시 아미노산 threonine, isoleucine 및 valine의 첨가가 3-hydroxybutyrate (3HB)와 3-hydroxyvalerate (3HV)를 단위체로 하는 공중합체 [poly(3HB-co-3HV)]의 생합성에 미치는 영향을 조사하였다. 세가지 아미노산은 3HV의 전구물질로 매우 효과적이어서 생성되는 공중합체내 3HV 함량을 크게 증진시켰다. 발효조에서의 배양시 3HV 함량은 첨가되는 아미노산 농도에 따라 증가하였으며, 20 mM과 30 mM의 threonine을 배양액에 첨가하였을 때 합성된 공중합체내 3HV 최종함량은 각각 19 mol%와 23 mol%에 달하였다. 그러나 단일탄소원으로서의 아미노산은 미생물의 증식이나 3HB 단위체 합성에 효과적으로 이용되지 못하였다. 아미노산을 효율적으로 3HV로 전환할 수 있는 Alcaligenes sp. K-912의 생리적 특성을 고려해 볼 때, 아미노산 대사기능이 변형된 본 균주의 변이주를 이용함으로써 단일탄소원으로부터 3HV 함량이 높은 poly(3HB-co-3HV)를 효과적으로 생산할 수 있을 것으로 기대된다. Effects of amino acids, such as threonine, isoleucine, and valine, on the biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer, poly(3HB-co-3HV), in Alcaligenes sp. K-912 were investigated. The amino acids were effective precursors of 3HV which led to increase the molar fraction of 3HV in the copolymer. The 3HV content in the copolymer increased with increasing concentrations of the amino acids. In fermentor cultures, the final 3HV content reached 19 mol% at 20 mM threonine, and 23 mol% at 30 mM threonine. However, relatively low yields of both dry cell and copolymer were obtained when the amino acids were used as the sole carbon source. The present results provide the possibility of producing poly(3HB-co-3HV) with high 3HV content from single carbon sources by modifying amino acid metabolic pathways of Alcaligenes sp. K-912.

Classical Swine Fever (Hog cholera) Virus 약독순화주 (Suri 주)의 gp55 Gene 염기서열 분석

김귀현,장경수,강경임,이병형,박종현,안수환,전무형 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

An attenuated classical swine fever virus (CSFV), Suri strain, is a variant derived from a vaccine virus, LOM strain. This study was performed to elucidate the molecular biologcal properties of CSFV Suri strain, and to obtain the basic data for molecular epidemiological approaches for the disease. The truncated form of gp55 gene without the C-terminal trasmembrane domain, in size of 1,023bp, was amplified by RT-PCR and sequenced by dye terminator cyclic sequencing method, and inserted into BamHI site of pAcGP67B baculovirus vector, establishing a cloned pAcHEG plasmid. By the nucleotide sequences determined, 341 amino acid sequences were predicted. As compard the nucleotide and amino acid sequences of gp55 of Suri with the various CSFV, Suri strain showed the high homology over 99.1% with ALD and LOM strains, but comparably the lower homology with Alfort and Brescia. In comparison of amino acid sequence in variable domain of gp55 protein, the similar tendency of homology was observed. In hydrophobicity analysis, all of four CSFV strains revealed the analogous patterns of hydrophobicity. The numbers and locations of N-glycosylation site and cysteine residuse in gp55 were analyzed, those of Suri strain being coincident with ALD and LOM strains. The results suggest that gp55 in Suri strain has the high similarity to those in ALD and LOM strains in terms of the nucleotide and amino acid sequences and the functional properties of gp55 protein.

한국전통 식품의 원료인 메주와 누룩에서 분리된 접합균에 대한 연구

유기원,성창근,이상선,유진영 충남대학교 생물공학연구소 1998 생물공학연구지 Vol.6 No.-

The fungal isolates of Mucorales, directly collected from Korean traditional raw materials of Nuruk (raw material for Korean rice wine) and Meju (raw material for Korean soysauces), were compared with those of Rhizopus oryzae purchased. The fungal isolates of Rhizopus, Mucor, and Absidia mostly identified as based on the morphological observations, were evaluated with the PCR-polymorphic bands. The PCR-polymorphic bands of the genomic DNA reacted with the primers of OPD series tenmer were various, but showed averaged 4 to 6 in the agarose-electrophoresis. The dissimilarity coefficient (DC) between two isolates were compared by the cluster analysis, dendrogams and polar ordinations. The isolates of R. oryzae known showed several groupings within the lower value of DC and were divided to two groups of amylo-process and other fungi with other purposes. The isolates unidentified were identified by the DC made of this results. Taxonomy of these isolates made by the morphological observations were consistent with those resulted above in most case but not in all aspects. More works were needed with the isolates known for detail informations of Mucorales.