DJ-1/park7 modulates vasorelaxation and blood pressure via epigenetic modification of endothelial nitric oxide synthase

Won, Kyung Jong,Jung, Seung Hyo,Jung, Soo Hyun,Lee, Kang Pa,Lee, Hwan Myung,Lee, Dong-Youb,Park, Eun-Seok,Kim, Junghwan,Kim, Bokyung Oxford University Press 2014 Cardiovascular research Vol.101 No.3

<P><B>Aims</B></P><P>DJ-1/park7, a multifunctional protein, may play essential roles in the vascular system. However, the function of DJ-1/park7 in vascular contractility has remained unclear. The present study was designed to investigate whether the DJ-1/park7 is involved in the regulation of vascular contractility and systolic blood pressure (SBP).</P><P><B>Methods and results</B></P><P>Norepinephrine (NE) elevated contraction in endothelium-intact vessels in a dose-dependent manner, to a greater extent in DJ-1/park7 knockout (DJ-1/park7<SUP>−/−</SUP>) mice than in wild-type (DJ-1/park7<SUP>+/+</SUP>) mice. Acetylcholine inhibited NE-evoked contraction in endothelium-intact vessels, and this was markedly impaired in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP>. Nitric oxide (NO) production (82.1 ± 2.8% of control) and endothelial NO synthase (eNOS) expression (61.7 ± 8.9%) were lower, but H<SUB>2</SUB>O<SUB>2</SUB> production (126.4 ± 8.6%) was higher, in endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice than in those from DJ-1/park7<SUP>+/+</SUP> controls; these effects were reversed by DJ-1/park7-overexpressing endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice. Histone deacetylase (HDAC)-1 recruitment and H3 histone acetylation at the eNOS promoter were elevated and diminished, respectively, in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls. Moreover, SBP was significantly elevated in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls, but this elevation was inhibited in mice treated with valproic acid, an inhibitor of Class I HDACs including HDAC-1.</P><P><B>Conclusion</B></P><P>These results demonstrate that DJ-1/park7 protein may be implicated in the regulation of vascular contractility and blood pressure, probably by the impairment of NO production through H<SUB>2</SUB>O<SUB>2</SUB>-mediated epigenetic inhibition of eNOS expression.</P>

한국 국립공원 산림생태계의 수목 탄소저장량 평가

이상진,박홍철,박관수,김현숙,이창민,김진원,심규원,최승운,Lee, Sang-Jin,Park, Hong-Chul,Park, Gwan-Soo,Kim, Hyoun-Sook,Lee, Chang-Min,Kim, Jin-Won,Sim, Gyu-Won,Choi, Seung-Woon 한국환경복원기술학회 2022 한국환경복원기술학회지 Vol.25 No.3

The purpose of this study is to quantitatively evaluate the amount of carbon storage for trees in forest ecosystem to support the foundation for carbon neutrality implementation in Korea National Park. It targeted 22 national parks designated and managed as national parks in Korea, and conducted research on forest trees in the terrestrial ecosystem among various natural and ecological carbon sink. The survey and analysis method followed the IPCC guidelines and the National Greenhouse Gas Inventory in Korea. The amount of tree carbon storage in the forest ecosystem of Korea National Park was confirmed to be about 218,505 thousand CO₂-ton and the amount of carbon storage per unit area was 570.8 CO₂-ton per hectare. Compared to 299.7 CO₂-ton per hectare, the average carbon storage per unit area of the entire Korean forest, it was found that about twice as much carbon was stored when assuming the same area. In other words, it means that the tree carbon storage function of the national park is about twice as high as that of the average tree carbon storage function of entire Korean forest. It has great implications in Korea National Park not only provides biodiversity promotion and exploration services as a national protected area, but also performs excellent functions as a carbon sink.

Rifampicin에 의한 것으로 추정되는 위막성 대장염1예

김수현,이은우,정종혁,문승현,김동한,양혁승,오영상,김호동,김도현,박혁,박정환,박경옥,이영직 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.2

Pseudomembranous colitis, caused by altering the normal colonic flora and allowing the multiplication of Clostridium difficile, is an deleterious adverse effect of antibiotics. But it is rarely reported by rifampicin. Rifampicin is one of the first line drug in the treatment of tuberculosis and many patients are exposed to its potential adverse effects. We experienced a patient that had abdominal discomfort and hematochezia due to pseudomembranous colitis after receiving antituberculous medication, and which was probably caused by rifampicin. A 82 years old man was admitted with abdominal discomfort and hematochezia for one week. On the past history he had been diagnosed as endobronchial tuberculosis about 4 months ago. Colonoscopy revealed multiple discrete whitish mucosal lesion on rectosigmoid colon, and histologic findings were consistent with pseudomembranous colitis. The antituberculous agents were discontinued and vancomycin was administered. The patient's symptoms were resolved within several days. There was no recurrence after reinstitution of the antituberculous agents excluding rifampicin. We report here on a case of pseudomembranous colitis probably due to rifampicin.

반려동물의 기생충검사 이론 및 실제

이성호,박영석,박병권,김상기,정지윤,박수진,박창식,김병수 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

The aim of this article is to improve human health and that of companion animals by improving the diagnosis and control of parasitic diseases. This is achieved by research into parasites of veterinary significance including zoonoses and the use of comparative systems to increase our understanding of some human parasitic diseases. We should promotes learning and teaching through knowledge and operates a diagnostic unit that offers a service to pet owner for indigenous and exotic parasitic diseases of companion animals. We must diagnose and treat them early. Zoonotic parasites are very important because many of animal parasites are zoonoses and give pain and death to human. Parasites are divided Protozoa, Helminthes and Arthropods. There are many protozoa, about 65,000 species. Arthropods are the vector of another diseases, such as virus, bacteria, protozoa and nematodes. Helminthes are composed of Trematoda, Cestoda, Nematoda and Acanthocephala. The target of Veterinary Parasitology is to recognize the characteristics of these parasites and subsequently diagnose, treat and control them. This article is intended to familiarize you with the essential facts and concepts of companion animal parasitology. Emphasis is placed on fecal sample techniques (comparison of fecal examination techniques; frequently found artifacts in fecal smears; direct Smear; zinc sulfate flotation; etc.) We recommend that the study be done to more references on animal parasites.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

관동맥질환에서 저밀도 지단백 입자 크기의 분포

임효영,김인숙,승기배,강동헌,전두수,장기육,박준철,이수영,홍순조,최규보 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.8

기관골 신생증 1예

오영상,이은우,정종혁,문승원,김수현,김동한,양혁승,박정환,박경옥,강성수,이영직 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.2

Tracheobronchopathia osteoplastica is a rare disorder of unknown cause characterized by the projection of numerous bony or cartilaginous submucosal nodules into the tracheobronchial lumen with sparing of the posterior membranous portion of tracheobronchial tree. In the past, most of the cases were diagnosed as secondary finding at autopsy. Nowaday, the key component of the evaluation is flexible fiberoptic laryngoscopy and bronchoscopy, which typically demonstrates irregular spicules of subrnucosal bone and cartilage projecting into the tracheobronchial lumen and causing various degrees of airway obstruction. A 45 years old man was visited Sunchon St. Carollo hospital due to discomfort on throat. We saw several nodules just below vocal cord via laryngoscope. The computed tomography revealed multiple small calcified nqdules on trachea and both main bronchi. Pathologic finding of bronchoscopic biopsy showed abnormal proliferation of bony and cartilagious nodules in the tracheal submucosa. These fingdings were consistent with tracheobronchopathia osteoplastica. We report here on a case of tracheobronchopathia osteoplastica with review of literature.

시각장애인의 유산소성 운동이 체력에 미치는 영향

박재성,김주혁,황영성,김도호,이수판,한성유,허정석 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.4

This research fulfilled the program of aerobic exercise for living during 6 months with seven men and women who are disabled people with visual impairment, The program consists or gym, swimming, aerobic and running, During 6 months, all exercises were executed 1 hours, twice a week for 6 weeks. Each exercise had self-servant and was carried out 60 minutes with teaching method of special directors in every weep Physique by height, weight, condition of exercise ability by the capacity of the lungs of the heart and lung endurance and intake of the maximum oxygen, muscular strength of stomach, grasping power, pliability by forward bending with the upper part of the body, instantaneous force of muscle in a sudden jump, muscular endurance by put-up and sit-up, agility by side-step and response of the whole body, and balance by one leg standing while closing eyes as using Helmas (sewoo systems korea) were measured. The Results, 1.There is a significant difference for both men and women in the capacity of the lungs and the heart and lung endurance, However only women have a significant difference in intake of the maximum oxygen. 2. Both men and women have a significant difference in muscular strength of stomach and grasping power to measure muscular strength. 3. Both men and women have a significant difference in forward bending with the upper part or the body to measure flexibility. 4. Sudden jump to measure instantaneous force of muscle, sit-up, and push-up to measure muscular endurance show that both men and women have a significant difference.(p<.01) 5. Side-step and response of the whole body to measure agility show that both men and women have a significant difference. 6. One leg standing with closing eyes to measure balance show that both men and women have a significant difference.

카제인 전사조절부위와 결합하는 자궁조직 전사인자의 특성

박상규,이승환,최인호,이창수 건국대학교 자연과학연구소 1998 建國自然科學硏究誌 Vol.9 No.2

생쥐 β-카제인 promoter내 TGAT/ATCA의 palindrome 구조는 유선의 핵단백질과 상호작용을 하며, 이 부위는 progesterone의 작용에 의해 promoter활성을 억제한다. Progesterone의 조절을 받는 주요 조직으로 알려진 유선과 자궁으로부터 핵단백질을 추출하여 palindrome 구조와 상호 결합하는 핵단백질을 gel shift assay를 통해 분석하였다. 유선과 자궁의 핵단백질들이 생쥐 B-카제인 promoter(-380/-263)에 결합하는 한 개의 DNA-단백질 복합체가 검출되었다. 두 조직간의 검출 밴드의 크기는 전기영동의 이동상에서 동일함을 보였다. 이 복합체는 TGAT/ATCA 염기서열을 갖는 wild-type oligonucleotide의 첨가로 형성되지 않았으나 mutant-type의 첨가는 밴드 형성에 영향을 미치지 않았다. 이와 같이 유선과 자궁 두 조직에 있어서 DNA 결합 복합체의 이동도가 동일하였고 이 복합체가 palindrome 염기서열과의 상호 결합에 의해 형성됨을 알 수 있었다. 한편 TGAT/ATCA 염기서열과 결합하는 단백질의 cDNA 클론을 자궁에서 유래된 λgtII cDNA expression library로 부터 screening 하였다. 일차 screening으로부터 9개의 클론들이 합성된 wild-type probe를 사용하여 얻어졌다. 이 중 한개의 클론만 2차와 3차 hybridization을 거쳐 최종 screening 되었다. 이 클론은 비특이적인 mutant-type probe를 사용하여 검증되어졌다. 클론된 재조합 phage DNA를 제한효소 EcoR I으로 처리한 결과 전사인자 cDNA의 길이가 2.3kbp임을 확인하였다. A palindromic structure, TGAT/ATCA, of mouse β-casein promoter interacts with nuclear factor of mammary gland, and this element is required for the inhibition of promoter activity by the action of progesterone. The nuclear factor binding to the paindromic structure was compared by a gel shift assay in uterus and mammary tissue, which are major target tissue to progesterone. One DNA-protein complex was detected with nuclear extracts of both uterus and mammary tissue by using the mouse β-casein promoter (-380/-263). The complex showed idential electrophoretic mobility in both uterus and mammary tissues. The formation of complex was eliminated by the addition of synthetic wild-type oligonucleotide with TGAT/ATCA sequence, but not that of mutant type without TGAT/ATCA sequence. The results from the identical mobility of complex and competition gel shift assay showed that a common nuclear factor binding to the palindromic sequence was present both in uterus and mammary tissues. A uterus cDNA clone encoding a TGAT/ATCA sequence-binding protein was screened from a uterus-derived λgtII cDNA expression library. Nine recombinant phages were obtained from the primary screening using a synthetic wild-type probe. One distinct positive clone was detected after the secondary and tertiary screening. Nonspecific mutant-type probe was used to confirm the authenticity of recombinant phages. The digestion of recombinant DNA with EcoR 1 showed that the positive clone contained a 2.3kb cDNA.

高級經營者精報시스템 計劃 및 設計方法論 分析

朴慶洙,金勝運 全北大學校 1992 論文集 Vol.34 No.-

Information systems are designed around information requirements or needs. However, most existing information systems do not have the capabilities required to support executives' information needs. Excecutive information systems are designed to gether, process, and displasy the information that help senior executives assess their organization's success. The successful implementation of EIS is dependent of efficient information system planning. But the correct and complete information requirements for effective EIS planning and design are frequently very difficult to obtain. In this paper, methodologies for EIS planning and design are analyzed and availability of a methodology is presented through the case study.