콩아이스크림의 상품화를 위한 고정화 효소의 이용

이숙영,이현옥,노숙령,김기숙,이복희,박미정 중앙대학교 생활과학연구소 2002 생활과학논집 Vol.15 No.-

The study was to investigate the functional properties of soy protein isolate hydrolyzed by immobilized α-chymotrypsin and the quality characteristics of soy ice cream made by the soy protein isolate treated with immobilized α-chymotrypsin. Up to the hydrolysis rate of 22.9%, treatement by immobilized α-chymotrypsin costed more time than that of α-chymotrypsin. Foam and emulsion capacity were decreased by the number of repetitive usage. Apparent viscosity and overrun of soy ice cream treated with immobilized α-chymotrypsin and α-chymotrypsin were shown similar tendency. In the sensory characteristics, soy ice cream by enzyme treatment was evaluated as much acceptable than untreacted soy ice cream in the item of beany flavor, icy feel and overall quality. For efficient use of immobilized α-chymotrypsin industrially, α-chymotrypsin immobilized on glass beads can be reused for about 11 times more than α-chymotrypsin.

대학생들의 떡에 대한 기호도 및 이용현황

이현옥,노숙령,김기숙,이숙영,이복희,박미정 중앙대학교 생활과학연구소 2002 생활과학논집 Vol.15 No.-

The survey study was conducted to investigate the rice cake consumption pattern including the frequency of intake and the degree of preference and the area of improvement for Korean rice cake among college students. About the motive for purchases of rice cake, 'taste is good(43.3%)' was the highest, and 'eat as a snack' the next (42.9%). The reason which does not eat the rice cake frequently was uncomfortable to eat (24.1%), insipid(21.4%), not variously assorted(17.9%) in the order named. The college students surveyed were eating rice cake as a snack mainly. They wanted the rice cake to be charmed and be in pretty shape, and fusion style for gift. And they wanted to be rice cake more sweetened and cool taste like ice cream. Therefore, traditional rice cake is required for further research for the development of product which is more acceptable for the young adults.

관동맥질환에서 저밀도 지단백 입자 크기의 분포

임효영,김인숙,승기배,강동헌,전두수,장기육,박준철,이수영,홍순조,최규보 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.8

Substance P restores normal skin architecture and reduces epidermal infiltration of sensory nerve fiber in TNCB-induced atopic dermatitis-like lesions in NC/Nga mice

Choi, Hyeongwon,Kim, Dong-jin,Nam, Seungwoo,Lim, Sunki,Hwang, Jae-Sung,Park, Ki Sook,Hong, Hyun Sook,Won, Younsun,Shin, Min Kyung,Chung, Eunkyung,Son, Youngsook Elsevier 2018 JOURNAL OF DERMATOLOGICAL SCIENCE Vol.89 No.3

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Atopic dermatitis (AD) is a chronic inflammatory skin disorder characterized by intense pruritus and eczematous lesion. Substance P (SP) is an 11-amino-acid endogenous neuropeptide that belongs to the tachykinin family and several reports recently have supported the anti-inflammatory and tissue repairing roles of SP.</P> <P><B>Objective</B></P> <P>In this study, we investigated whether SP can improve AD symptoms, especially the impaired skin barrier function, in 2, 4, 6-trinitrochlorobenzene (TNCB)-induced chronic dermatitis of NC/Nga mice or not.</P> <P><B>Method</B></P> <P>AD-like dermatitis was induced in NC/Nga mice by repeated sensitization with TNCB for 5 weeks. The experimental group designations and topical treatments were as follows: vehicle group (AD-VE); SP group (AD-SP); and SP with NK1R antagonist CP99994 (AD-SP-A) group. Histological analysis was performed to evaluate epidermal differentiation, dermal integrity, and epidermal nerve innervation in AD-like lesions. The skin barrier functions and pruritus of NC/Nga mice were evaluated by measuring transepidermal water loss (TEWL) and scratching behavior, respectively.</P> <P><B>Result</B></P> <P>Topical SP treatment resulted in significant down-regulation of Ki67 and the abnormal-type keratins (K) K6, K16, and K17, restoration of filaggrin and claudin-1, marked reduction of TEWL, and restoration of basement membrane and dermal collagen deposition, even under continuous sensitization of low dose TNCB. In addition, SP significantly reduced innervation of itch-evoking nerve fibers, gelatinase activity and nerve growth factor (NGF) expression in the epidermis but upregulated semaphorin-3A (Sema3A) expression in the epidermis, along with reduced scratching behavior in TNCB-treated NC/Nga mice. All of these effects were completely reversed by co-treatment with the NK1R antagonist CP99994. In cultured human keratinocytes, SP treatment reduced expression of TGF-α, but upregulated TGF-β and Sema3A.</P> <P><B>Conclusion</B></P> <P>Topically administered SP can restore normal skin barrier function, reduce epidermal infiltration of itch-evoking nerve fibers in the AD-like skin lesions, and alleviate scratching behavior. Thus, SP may be proposed as a potential medication for chronic dermatitis and AD.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Topical Substance P treatment reduced AD-like symptoms in TNCB-induced NC/Nga mice. </LI> <LI> Topical Substance P treatment reduced epidermal acanthosis and expressed normal keratin profiles in AD-like mice. </LI> <LI> Topical Substance P treatment regulated both small nerve attraction and repulsion factors in AD-like skin. </LI> <LI> Topical Substance P treatment recovered skin barrier function and reduced pruritus in AD-like mice. </LI> </UL> </P>

Vancomycin내성 Enterococci의 빈도 조사 및 중합효소연쇄반응을 이용한 유전자형의 분석

홍기숙,강은숙,이미애 대한임상병리학회 1998 대한임상병리학회지 Vol.18 No.3

산성화가 식물플랑크톤 종조성에 미치는 영향

채기숙,김범철 江原大學校 附設 環境硏究所 1997 環境硏究 Vol.14 No.-

The effects of acidification on phytoplankton composition were observed by enclosure experiments. The surface water of Lake Soyang was acidified to 5.5, 5.0, or 4.5 with H₂SO₄, and the change of species composition and biomass of phytoplankton were observed. Axenic cultures of two algae, a green alga(Scenedesmus obliquus) and a cyano bacterium(Microcystis aeruginosa) was also used to test the effects of acidification. Dominant phytoplankton in Lake Soyang were Asterionella in winter, and peridinium and Anabaena in summer. In acidified condition, dominant species changed from cyanobacteria to green algae. At pH 4.5, most algal cells showed morphological damage. Species richness decreased with decreasing pH. The growth rate of Anabaena declined at below pH5.5. Whereas, the growth rate of Peridinium, Fragilaria, and Asterionella decreased only at pH 4.5. Green alga, Desmidium and Dictyosphaerium, were Anabaena, green algal increased even at pH 4.5 and grew slower at neutral pH. In axenic cultures, the growth of Microcystis aeruginosa was inhibited at 4.0 and 5.0 and showed maximum at pH 9.0. Scenedesmus obliquus also grew well at pH 5.0 and showed maximum at 9.0 At pH 4.0, Scenedesmus did not grow at all.

고등학생의 열등감과 학업적 자기효능감을 위한 Adlerian 집단상담의 효과 분석

권기양,심혜숙 부산대학교 과학교육연구소 2005 교사교육연구 Vol.44 No.-

The purpose of this study is to investigate the effects of Adlerian Group Counseling Program which was based on individual psychology on inferiority feelings and academic self-efficacy of the academic high school students. For this study, the following hypotheses were established. First, there would be significant decrease in inferiority symptoms including three subordinate factors (sensitiveness, negativeness, retreat) for high school students after the treatment. Second, there would be significant increase in academic self-efficacy including three subordinate factors (self-confidence, self-regulatory efficacy, task difficulty preference) for high school students after the treatment. The subjects of this study were 27 first grade students of academic high school in Busan. 12 students were assigned to an experimental group and 15 were assigned to a control group. The study progressed through three stages : pre-test, experimental intervention (participation in the program by the experimental group), post-test. The experimental group participated in 15 sessions of 50 minutes each over six months. Inferiority Symptom scale (Yim, Gie-Hong) and Academic Self-efficacy scale (Kim Ah-yeong, Park In-yeong) were administrated in both experimental and control group.The results from this study were as follows; First, Adlerian Group Counseling Program significantly decreased the total scores in inferiority symptoms and the each score in three subordinate factors (sensitiveness, negativeness, retreat) for the experimental group subjects. Second, Adlerian Group Counseling Program significantly increased the total scores in the academic self-efficacy and the each score in three subordinate factors (self-confidence, self- regulatory efficacy, task difficulty preference) for the experimental group subjects. Based on the above results, it can be concluded that Adlerian Group Counseling Program is very effective in helping the high school students reduce the level of inferiority symptoms and elevate the level of their academic self-efficacy. Therefore, Adlerian Group Counseling Program can be strongly suggested as a useful means for high school students' self-growth and improvement for their fruitful school lives.

청소년 정신분열증 환자의 어머니의 단기집단 정신치료의 연구

곽영숙,백기청,고기형 大韓神經精神醫學會 1991 신경정신의학 Vol.30 No.1

To evaluate schizophrenic mothers' conflict, depression, and attitude toward their schizophrenic children and how they perceive psychiatric illness, we studied group process of nine mothers in twelve sessions. The results were as follows : 1) There was significant difference in only atmosphere of family in PBR. 3) There was partial changes in cause, treatment and perspective of disease. 4) Drop-out rate was 10.1%. 5) Late attendance rate was 2.8 persons per session and abscence rate was 0.8 person per session. 6) Group silence was 0.9 frequency per session and there was great difference to authority and style of therapist. 7) Laughter rate and crying rate were 0.6 and 0.4 frequency, respectively. 8) Familial attendance was 0.3 person per session except mothers. 9) There was overt depression during group psychotherapy. 10) Uncooperation of therapist made group process to involve only therapist. 11) Goup cohesion and universality were major group dynamics, which dependency and helplessness appeared.

정보화 사회와 인간성 교육 : 코메니우스의 교육사상을 중심으로 centering on Comenius' Educational Thought

김기숙 한국기독교교육정보학회 2001 기독교교육정보 Vol.3 No.-

The modern science, the very matrix of today's information society has its foundation in the dualism thought which excludes supernatural knowledge since Descartes showed up Such development of science has caused the problems of dehumanization like man's estrangement from the God, the origin of existence, and led to a false logic that only practical and physical knowledge can be scientific. In addition, the thinking way of dualism which separates subject from object has resulted in separating faith from reason, and religious knowledge from secular knowledge Thus, partial and fragmented knowledge could not be related to the whole area of different knowledges, though it has been profoundly researched in its individual field, This paper aims to reveal that the partial and fragmented knowledge in the information age is essentially the product of dualism thought, and to try to find an alternative from Comenius' educational thought in order to solve the problems. First, the Christian education in the information age should start from re-finding the God, the origin of existence. The knowledge of our age which causes man to think himself powerful and as an absolute ruler over the nature should be substituted for chresis which helps man recognize himself as a fragile creature by the God, and return all the glory to the God by knowing the origin and goals of all things. The partial and fragmented thought structure which has hardly reciprocal harmony in relation to the whole knowledge in the information age, should be based on Comenius' concept of "Pan", and converted to a whole thought structure in relation with the God. This article adopts Comenius' concept of "Pan" as an educational concept for achieving such mutually related and whole thought. Comenius' educational thought reveals that the educational engineering emphasized in the age of information is means to let human being recover the God's image, but never an end itself. From the same viewpoint, Christian education should be focused on finding the God, the source of wisdom, and rebuilding humanity complying to the God's image, rather than producing efficient helpers in the given social system and structure.

유치 수복물에 따른 주변 법랑질의 탈회 저항성 : QLF를 이용한 연구

권해숙,현홍근,김영재,김정욱,장기택,김종철,한세현,이상훈 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.1

본 연구에서는 유치 수복물의 불소 방출 여부에 따른 주변 법랑질의 탈회 저항성과 재광화 효과를 살펴보고자 하였다. 건전 유전치 48개를 16개씩 임의로 세 군으로 나누어, FiltekTM Z250(1군), F2000(2군), KetacTM N100(3군)을 사용하여 제조사의 지시에 따라 충전하였다. 3일 동안 인공 우식 병소를 유발한 후, 14일 동안 인공 타액에 담가 재광화를 유발하였다. Quantitative light-induced fluorescence(QLF)를 이용하여 실험 단계와 시간에 따른 무기질 소실량(ΔQ)의 변화를 관찰한 결과는 다음과 같다. 1. 탈회가 일어난 인공 우식 병소의 ΔQ 값은 3군, 2군, 1군 순으로 작게 나타났고, 이 중 3군은 1군에 비해 유의한 탈회 저항성을 보였다. 2. 1군, 2군, 3군 모두 재광화 1일 후부터 초기 인공 우식 병소의 ΔQ 값과 비교 시 유의한 차를 보였으며, 관찰 기간 동안 지속적인 증가 양상을 나타내었다. 3. 재광화 속도를 의미하는 Δ(ΔQ)/일 값은 각 군에서 처음 1일 동안 유의성 있게 크게 나타났으며, 그 이후 급속히 감소하였다. 4. 수복 재료에 따른 재광화 정도는 각 군 간에 유의한 차이가 없었다. The objective of this in vitro study was to detect and monitor demineralization and remineralization of primary teeth according to restorative materials using quantitative light-induced fluorescence (QLF). A single bur hole was drilled on the each sound forty eight primary anterior teeth, and the specimens were divided into three groups. The cavity was restored with FiltekTM Z250(Group 1), F2000(Group 2), KetacTM N100(Group 3) following the manufacturer's instructions. The teeth were subjected to the demineralizing buffer for 3 days, and then subjected to a remineralizing buffer for 14 days. The change of mineral loss(ΔQ) according to the stages was evaluated by QLF and the following results were obtained: 1. When demineralization was done, ΔQ was increased as follows. : Group 1 (-110.79 ± 27.77) < Group 2 (-104.84 ± 28.95) < Group 3 (-90.16 ± 21.87) : Resistance to demineralization was statistically significant in Group 3. 2. There was a statistically significant increase in ΔQ of all groups since 1st day of remineralization 3. The rate of remineralization, Δ(ΔQ)/day, showed significant high value in each group on the 1st day then decreased rapidly. 4. There was no statistically significant difference in the degree of remineralization among restorative materials.