임플란트 완성 환자의 구강건강관련 삶의 질과 관련 요인

박정현,엄상화,권현숙,조갑숙,허승주,이소영,유병철,Park, Jung-Hyun,Urm, Sang-Hwa,Kwun, Hyeon-Sook,Cho, Gab-Suk,Heo, Seung-Ju,Lee, So-Young,Yu, Byeng-Chul 한국치위생학회 2011 한국치위생학회지 Vol.10 No.2

Objectives : This study was purposed to estimate the level of oral health quality of life and the relationship between health-related factors and oral health quality of life in patients with successful dental implants. Methods : This questionnaire survey was conducted during the period from December 2009 to March 2010 with 126 patients confirmed successful dental implants in Busan. The oral health related quality of life and oral health-related factors were estimated with OHIP-14 and 8 oral health characteristics, respectively. Data analysis was performed with descriptive analysis, t-test, ANOVA, multiple regression analysis using SAS(ver 9.1) program. Results : The score of oral health quality of life in patients with successful dental implants was $12.25{\pm}7.82$. The scores of subscale of the oral health quality of life were $1.24{\pm}1.35$ in social disability, $1.60{\pm}1.37$ in handicap, $1.60{\pm}1.44$ in psychological disability, $1.70{\pm}1.38$ in functional limitation, $1.81{\pm}1.35$ in physical disability, $2.14{\pm}1.46$ in physical pain, $2.17{\pm}1.46$ in psychological discomfort, respectively. The related factors of oral health quality of life in patients with successful dental implants were absence of other type prosthesis and experience of tooth brushing education. Conclusions : The oral health related quality of life in patients with successful dental implants was relatively good condition. Social supports and chances for high quality denture and tooth brushing educations are needed to improve oral health related quality of life in patients with dental implants.

Application of Fluorescence Polarization Immunoassay for the Screening of Ochratoxin A in Unpolished Rice

박정현,정덕화,이인선,Park, Jung-Hyun,Chung, Duck-Hwa,Lee, In-Seon Korean Society of Life Science 2006 생명과학회지 Vol.16 No.6

To High Throughput Screening (HTS), a homogeneous fluorescence polarization immunoassay (FPIA) was developed for the quantitative determination of ochratoxin A(OTA) using a $Victor^3$ (PerkinElmer). The homologous tracer, fluorescein-labelled OTA-EDF were synthesized and a specific OTA antibody has been used in the development of the method. It allowed the determination of OTA in the concentration range 0.5-200 ng/ml, with the detection limit of 0.3 ng/ml. The method developed was highly specific and reproducible. OTA spikes in unpolished rice extracts were determinable by FPIA with good recovery. For naturally contaminated unpolished rice samples some disagreement was observed between the results obtained by FPIA and HPLC, which could be related to the a little matrix effect observed for FPIA. Further research is needed to validate the procedure. On the basis of these initial results, this FPIA appears to meet the performance criteria for OTA screening of food samples without a complicated clean-up. 식품안전에 대한 관심이 증가되고 있는 현재, 생물학적 화학적 위해요소로 분류되고 있고, 현재 많은 나라에서 규제치를 설정하고 있는 곰팡이 독소인 ochratoxin A(OTA)에 대한 정량적 측정이 가능한 고속검색법을 개발 하고자 단클론성 항체를 이용하여, 측정시 분리과정이 필요 없는 형광편광면역분석법(FPIA)을 개발하고 최적화 시켰다. 동일구조를 가지는 형광물질 표식자인 OTA-EDF를 합성하여 OTA에 대한 특이항체와 경쟁반응을 시켜 나타나는 형광-편광도(mP)의 변화를 측정하였다. 이는 면역분석법의 특이성과 민감성을 충분히 만족하였다. OTA의 검출범위는 0.5-200 ng/ml였고, 검출한계는 0.3 ng/ml였다. 개발된 분석법은 다른 곰팡이 독소들과의 교차반응은 없었고 높은 특이성과 재현성 및 회수율을 나타내었다. HPLC 방법에 의한 회수율은 88-84%로 다소낮게, FPlA법의 회수율은 90-110%로 다소 높게 나타났다. 16점의 현미시료를 분석하였을 때, 2점이 상관관계가 높게 12-20 ppb 정도 오염된 것으로 나타났다. 4점은 FPIA 및 HPLC 모두에서 음성으로 판정되었다. 개발된 FPIA는 복잡한 전처리 방법이 필요 없는 신속한 검색이 가능하므로, 식품 및 환경에서의 OTA 잔류 검사에 유용하게 사용될 수 있을 것이다.

PSZ, Al₂O₃, TiO₂를 반응소결하여 제조한 쾌삭(快削) 세라믹스

박정현,정동식,이원재,김일수,Park, Jeong Hyun,Jung, Dong Sik,Lee, Won Jae,Kim, Il Soo 한국세라믹학회 2012 한국세라믹학회지 Vol.49 No.6

Machinability is important in engineering applications, especially in the current micro-electronics industry. Most ceramic components have complex shapes and hence require machining generally with diamond tools, which incurs a high production cost. Recently, h-BN-containing machinable ceramics have been developed, but these materials are very expensive due to the high raw materials and production costs. Therefore, the development of low-cost machinable ceramics is necessary. In this study, inexpensive $Al_2TiO_5$ was studied as a replacement for h-BN. $Al_2O_3$, $TiO_2$ and partially stabilized $ZrO_2$(PSZ) powders were mixed with various mole ratios and were sintered at $1500^{\circ}C$ for 1 h. The density, hardness and strength were then measured. The phase analysis and microstructures were observed by XRD and SEM, respectively. The machinability of each specimen was tested by micro-hole machining. The results of this research showed that the produced composites could be used as low-cost machinable ceramics.

각막문신술 후 각막 신경재생 및 지각의 변화

박정현,한영근,위원량,이진학,권지원,Jung Hyun Park,M,D,Young-Keun Han,M,D,Won-Ryang Wee,M,D,Jin-Hak Lee,M,D,Ji Won Kwon,M,D 대한안과학회 2007 대한안과학회지 Vol.48 No.7

Purpose: To investigate the changes of corneal nerve innervation and sensitivity after corneal tattooing. Methods: Twelve New Zealand rabbits were divided into two groups of six subjects each. Tissue marking dye or balanced-salt solution was injected into the corneal stroma in one eye of each subject. Corneal nerve innervation was observed at 3 days and 1, 2, 3, 4, and 6 weeks postoperatively and compared with that of the opposite eyes. Corneal sensitivity was evaluated at 1, 2, and 3 days and 1, 2, 3, 4, and 6 weeks postoperatively and compared to the preoperative values in the same subjects. Results: The ratios of corneal nerve density of the study eyes to those of the control fellow eyes were 1.09 in the central area and 1.06 in the peripheral area in subjects injected with tissue marking dye, and 1.11 and 1.23, respectively, in subjects injected with balanced salt solution, which showed no difference between the two groups. The corneal nerve density tends to increase at 3 days postoperatively (p=0.125), but no significant change was found thereafter. Corneal sensitivity assessed using the Cochet-Bonnet esthesiometer was 2.13±0.48 cm preoperatively. At three days postoperatively, it was 2.50±0.48 cm in subjects injected with tissue marking dye and 2.33±0.26 cm in subjects injected with balanced salt solution. It was significantly increased at 3 days postoperatively (2.42±0.36 cm, p=0.031), but there were no significant changes at 7 days or more postoperatively. Conclusions: Corneal nerve density increased at 3 days after corneal tattooing, and this effect is thought to be related to early postoperative hypersensitivity.

Stem Cell Based Strategies for the Treatment of Degenerative Retinal Diseases

박정현,구승엽,조명수,이학섭,최영민,문신용,유형곤,Park, Jung-Hyun,Ku, Seung-Yup,Cho, Myung-Soo,Lee, Hak-Sup,Choi, Young-Min,Moon, Shin-Yong,Yu, Hyeong-Gon The Korean Society for Reproductive Medicine 2010 Clinical and Experimental Reproductive Medicine Vol.37 No.3

망막 질환에서의 줄기세포 치료는 이전까지 치료가 불가능하다고 여겨졌던 환자들에서 시력을 향상시킬 수 있는 가능성 때문에 주목 받고 있다. 본문에서는 망막 전구세포의 분화를 위해 사용되는 태아 줄기세포, 배아줄기세포 및 성체줄기세포 등 다양한 세포 종류와, 내인적, 외인적 인자 및 이식 방법에 대해 논의하였다. 망막색소상피세포뿐만 아니라 시각세포 전구체로 성공적으로 분화시킨 실험적 연구가 보고되고 있다. 줄기세포기반치료는 아직 한계가 있지만 망막 질환 환자에서 시력을 회복하기 위한 보다 근본적인 치료 방법으로 기대되고 있다. Stem-cell therapy has the potential to improve vision in patients with untreatable retinal disease. Various types of cell source including fetal, embryonic and adult stem cells, intrinsic and extrinsic factors for differentiation into retinal progenitors and transplantation mode were discussed in this review. Experimental approaches have successfully induced photoreceptor precursor cells and retinal pigment epithelium. Stem-cell-based therapy is a promising treatment to restore vision in patients with retinal disease, in spite of the challenges.

낙동강 하구역 진우도 자연해빈의 지하수위 변동해석

박정현,윤한삼,이인철,Park, Jung-Hyun,Yoon, Han-Sam,Lee, In-Cheol 해양환경안전학회 2014 해양환경안전학회지 Vol.20 No.4

This study selected five observational stations in the normal direction of Jinu-do(island) shoreline and observed water temperature, electrical conductivity and pressure from March, 2012 to January, 2013(about 11 months) and attempted to see the variation characteristics of ground watertable. This study wants to know : 1) External environment force factors(tide, climate, wave etc.) affecting ground watertable variation through time series and correlation analysis. 2) Spatial variations of ground watertable and electrical conductivity change by storm event. First, we found that the station at the intertidal zone was strongly affected by wave and tide level and the stations at sand dune and vegetation zone was affected by precipitation and tide level through time series data and correlation analysis. Second, during the storm event, we found that ground watertable and electrical conductivity are stabilized at the start line of sand dune and vegetation zone and transition zone between freshwater layer and seawater layer exists in the experiment area and is about 50~70 m from coastline of the south side of Jinu-do(island). 본 연구는 낙동강 하구역의 사주섬 중 최서측에 위치하는 진우도를 대상으로 실험구를 구축한 후 해안선의 법선 방향으로 관측점 5곳을 선정하여 2012년 3월부터 2013년 1월까지 약 11개월간 관측된 수온, 전기전도도, 압력값을 이용하여 지하수위 변동해석을 하였다. 1) 지하수위의 수온, 염분, 수위변동과 조위, 파랑, 강수량 등의 외력조건과의 시계열 분석 및 상관분석을 통하여, 소상대 정점에서는 파랑 및 조위의 영향, 식생 및 모래지반 정점에서는 강수량 및 조위의 영향을 크게 받음을 알 수 있었다. 2) 고파랑내습시 관측점간의 지하수위 및 전기전도도의 공간적 거동을 살펴본 결과 사구(Sand dune) 및 식생대(Vegetation zone)가 시작되는 지점에서 담수층과 해수층의 천이 영역(Transition zone)이 존재하였고, 진우도 남측을 기준으로 해안선에서 50~70 m 사이에 위치함을 알 수 있었다.

사람 Mesenchymal stromal cell(hMSC) 분리를 위한 간소화된 방법에 대한 연구

박정현,김경화,이용무,구영,류인철,한수부,정종평,Park, Jung-Hyun,Kim, Kyoung-Hwa,Lee, Yong-Moo,Ku, Young,Rhyu, In-Chul,Han, Soo-Boo,Chung, Chong-Pyoung 대한치주과학회 2004 Journal of Periodontal & Implant Science Vol.34 No.1

많은 연구들에서 hMSC를 얻기 위해 centrifugation, fluoroscence activated cell sorter(FACS), magnetic activated cell sorter(MACS)가 이용되어져 왔다. 그러나 centrifugation만을 이용한 경우 순도가 떨어지며 FACS나 MACS의 경우에는 비용, 시간이 많이 드는 단점이 있다. 따라서 이 연구에서는 antibody cocktail을 이용하여 hMSC를 좀더 쉽게 얻어내는 방법에 대해 알아보았다. 사람의 골반에서 12G의 바늘을 이용하여 골수를 흡입한 후 heparin이 들어있는 시험관에 넣고 처리과정을 시행하기 전에 냉장고에 보관하며 가능한 한 빨리 처리 과정을 실시한다. 얻은 골수에 적당량의 RosetteSep( Stemcell Technologies)을 첨가한 후 실온에서 20분간 반응시킨다. 그 후 적당량의 Ficoll-paque위에 골수와 RosetteSep의 혼합물을 섞이지 않게 올리고 원심분리를 이용하여 원하는 세포층을 얻어낸다. 이 세포층을 따로 분리한 뒤 배양한다. 배양 시 세포가 80%이상 차기 전에 계속 passage를 시행하며 배양한다. 이는 세포가 밀도가 높아져 원치 않는 세포로 분화되는 것을 막기 위함이다. 배양된 세포가 다양한 분화능력을 가지고 있는지 알아보기 위해 세 가지로 분화를 유도하였다. 적절한 배지와 적절한 환경에서 배양함으로써 얻어진 세포를 osteoblast, chondroblast, adipocyte로 분화를 유도하였다. 분화된 세포가 원하는 형질의 세포로 분화되었는지를 확인하기 위하여 osteoblast의 경우 alizarin red staining, alkaline phosphatase activity, chondroblast의 경우 toluidine blue staining, adipocyte의 경우 Oil-Red-O staining으로 염색하여 분화를 확인하였다. 분리해낸 세포는 각각 세 가지 세포로 분화가 되었으며 이는 RosetteSep이 hMSC를 성공적으로 분리해냈다는 것을 보여준다. 그러나 모든 세포가 분화를 보이지는 않았으며 따라서 hMSC의 순도를 높이기 위한 연구가 더 필요하다. RosetteSep을 이용하면 다른 방법들 보다 쉽게 hMSC를 얻을 수 있으나 기존의 방법과 순도의 측면에서 더 비교할 필요가 있다.

수포각막병증에서 양막 이식술의 효용성

박정현,정진욱,위원량,이진학,김미금,이재림.Jung Hyun Park. M.D.. Jin Wook Jeoung. M.D.. Won Ryang Wee. M.D.. Ph.D... Jin Hak Lee. M.D. Ph.D... Mee Kum Kim. M.D.. Ph.D... Jae Lim Lee. M.D. 대한안과학회 2007 대한안과학회지 Vol.48 No.8

Purpose: To evaluate the efficacy of amniotic membrane (AM) transplantation in symptomatic bullous keratopathy using cryo-preserved or lyophilized AM. Methods: Eight bullous keratopathic patients, who experienced intractable pain despite medication and the application of a therapeutic lens, underwent permanent AM transplantation with 5 cryo-preserved and 3 lyophilized AMs following removal of epithelium using a trephine. The time to re-epithelization and the subsidence of pain were evaluated retrospectively. Results: Mean follow-up was 252±171.0 days. Pain relief and re-epithelization were obtained in all eight patients. The times to complete relief of pain was 32.2±22.7 days in patients with cryo-preserved AM transplantations (n=5) and 22.0±18.0 days in those with lyophilized AM transplantations (n=3) (p=0.549, Mann-Whitney U test). Respective times to re-epithelization were 25.4±20.7 days and 23.0±25.2 days (p=0.297). There were no significant differences in the diameter of transplanted AMs between the two groups (7.6±0.2 vs. 7.3±0.3 mm, p=0.172). Conclusions: Permanent AM transplantation effectively reduced the intractable pain caused by epithelial bulla. Whether the AM was cryo-preserved or lyophilized did not affect the efficacy of the treatment.

3D 프린팅 기술을 활용한 낙상충격 보호패드 구조설계 및 필라멘트 소재에 따른 특성 비교

박정현,정희경,이정란,Park, Jung Hyun,Jung, Hee-Kyeong,Lee, Jeong Ran 한국의류학회 2017 한국의류학회지 Vol.41 No.5

This study uses 3D printing technology to design and fabricate a fall impact protection pad with a spacer fabric structure. The design of the pads consists of hexagonal three-dimensional units connected in a honey-comb shape; in addition, the unit consists of a surface layer and a spacer layer. Protect pads were designed as either a hexagonal type or diamond type according to the surface layer structure; subsequently, a spacer filament was also designed as the most basic I-shape type. Designed pads were printed using four types of flexible filaments to select suitable material for a fall impact protection pad. Impact protection performance and bending stiffness were evaluated for the eight type of pad outputs. As a result of the impact protection performance evaluation, when the force of 6,500N was applied, the force passed through the pad was in the range of 1,370-2,132N. FlexSolid$^{(R)}$ and Skinflex$^{TM}$ showed good protection performance and cubicon flexible filament showed the lowest protection. NinjaFlex$^{(R)}$ was found to be the most flexible in the bending stiffness evaluation.

노년 여성을 위한 낙상충격 보호팬츠의 착의평가

박정현,이정란,Park, Jung Hyun,Lee, Jeong Ran 한국의류학회 2017 한국의류학회지 Vol.41 No.4

This study conducted a wearing evaluation of fall impact protective clothing developed in the previous study that evaluated activity, appearance, and practicality for elderly women. A total of 6 kinds of pants, which consisted of the three kinds of pads and two kinds of designs, were evaluated and the results are as follows. First, the activity of the protective clothing was tested with six women in their 60's and scored highly in most criterions that indicated satisfactory activity. The honeycomb type pad was superior in four of ten criterions for the activity evaluation results by pad types. Design B without a knee pad was evaluated as more comfortable on two motions that put pressure on the knee area. Second, the expert group considered design A to be better than design B, while the subject group preferred design B over design A in design appearance evaluation. Both the subject group and expert group evaluated that design A is better than design B in consideration of pads. Third, in the assessment of practicability, both the expert group and the subject group provided the highest score on the question of if elderly women needed protective clothing. The evaluation of washing ability indicated no noticeable change in the clothing form and size before and after washing.