UDCA를 함유한 생약추출물혼합제제의 항염효과에 관한 연구

류인철,김상년,정종평,Rhyu, In-Cheol,Kim, Sang-Nyun,Chung, Chong-Pyoung 대한치주과학회 1996 Journal of Periodontal & Implant Science Vol.26 No.4

There are many important factors in periodontal inflammation. $IL-1{\beta}$, $PGE_2$ and collagenase are predorminantly key factors. These inflammatory mediators induce gingival tissue and alveolar bone destruction. For the prevention and treatment of periodontal disease, it is necessary to inhibit $IL-1{\beta}$, $PGE_2$ production and collagenase activity. Ursodeoxycholic acid(UDCA) has immunomodulatory properties, and there is evidence that some natural extracts show antiinflammatory activity to some degree. The purpose of this study was to assess the inhibitory effect of UDCA and its mixture with natural extracts on $IL-1{\beta}$, $PGE_2$ production and collagenase activity. Accordingly we assessed the effect of UDCA and its mixture combined with some natural extracts on inhibition of $IL-1{\beta}$, $PGE_2$ production and collagenase activity. For the $IL-l{\beta}$ inhibition study, cultured cells were exposed to $25{\mu}g/ml$ LPS. $IL-1{\beta}$ activity was measured by $IL-1{\beta}$ enzyme immunoassay system. Human gingival fibroblasts were prepared and cells (l05/well) were seeded into culture plates. $rhIL-1{\beta}$ was added to induce $PGE_2$. The amount of $PGE_2$ in sample media was measured using enzyme immunoassay system. Crude collagenase was prepared from Porphyromonas gingivalis and collagenolytic activity was determined using a Collageno kit CLN-100. The test inhibitor was added to the assay mixture consisting of 0.1ml of 50mM Tris buffer(pH 7.5) and 0.2ml of substrate solution. UDCA and UDCA combined with natural extracts generally inhibited $IL-1{\beta}$ production. groups above 0.01% UDCA strongly inhibited $IL-l{\beta}$ synthesis. Both groups inhibited $IL-1{\beta}-induced$ synthesis of $PGE_2$. In low concentration, the degree of inhibition was as same as prednisolone. In high concentration, each group was superior to prednisolone. UDCA group and UDCA mixture group exerted a moderate inhibition of collagenolytic enzyme. The present study suggested that UDCA and its mixture with natural extracts could be further investigated as antiinflammatory drug for periodontal disease.

스크린도어(PSD) 설치 전 · 후의 전동차 실내공기질 평가

류인철,김창모,한규문,전재식,김주형,김민영 한국대기환경학회 2010 한국대기환경학회 학술대회논문집 Vol.2010 No.10월

교정을 이용한 치주치료

류인철 대한치과의사협회 1996 대한치과의사협회지 Vol.34 No.10

치주병인균과 화학요법제

류인철 대한치과의사협회 2000 대한치과의사협회지 Vol.38 No.11

친환경농산물의 유통체계 개선방안에 관한 연구 : Fuzzy AHP를 중심으로

류인철,최용석,이광배 한국산업경제학회 2012 산업경제연구 Vol.25 No.2

본 연구는 친환경농산물 유통체계의 개선방안에 관한 우선순위를 합리적으로 도출하기 위하여 Fuzzy-AHP를 사용하였다. 이를 위하여 친환경농산물 유통체계의 개선방안에 관해서 문헌고찰을 통하여 평가 항목을 선정하고, 이를 바탕으로 선행연구 및 유통전문가의 면접을 토대로 현재 겪고 있는 친환경농산물 유통체계의 문제점에 대한 개선방안들을 측정변수로 선정하였다. 그리고 Fuzzy-AHP를 통해 어느 평가항목이 가장 우선적으로 개선되어야 하는지 우선순위를 도출하였다. 분석결과, 상위 평가 요인의 4가지 항목 중, 유통주체가 32.6%로 최우선순위로 나타났고, 다음은 유통경로(28.1%), 산지유통(24.2%), 유통정책(22.7%) 순으로 나타났다. 그리고 측정변수 12가지의 평가기준 항목에서는 친환경 농산물 물류센터의 설치·운영(0.153)이 다른 개선방안들보다 가장 높게 나타났고, 다음으로는 직거래의 확충(0.117), 유통경로의 다양성 확보(0.116), 판매망 확충(0.113), 사이버 마켓 구축(0.102), 안정적인 수요처 확보(0.100) 등의 순으로 나타났다. 친환경농산물의 생산·확대, 수확 후 관리체계의 정비, 유통 품목의 다양화, 사이버마켓 구축자금지원, 직거래자금, 물류시설 지원자금 등의 평가항목 등은 비교적 낮게 나타났다. This study applied Fuzzy-AHP to make the most rational decision making on the improvement methods for an ambiguous distribution system of environment-friendly agricultural products. In this view, assessment items were selected through literature review to examine the methods to improve the distribution system of environment-friendly agricultural products. Then, the methods to improve the present distribution system problems of environment-friendly agricultural products were selected as measurement variables based on previous researches and interviews with distribution experts. Finally, top-priority improvement was decided among assessment items through Fuzzy-AHP. As a result of analysis, four priority assessment items were found. The top priority was the distribution subject(32.6%), followed by distribution channels(28.1%), agricultural product processing (24.2%), and distribution policies(22.7%). For assessment criteria items of 12 measure variables, the highest improvement method was the establishment and operation of the logistics center for environment-friendly agricultural products(0.153), followed by the expansion of direct transaction(0.117), guarantee of distribution channels(0.116), the expansion of sales networks(0.113), the construction of cyber markets(0.102), and the guarantee of stable demand(0.100). The production and increase of environment-friendly agricultural products, the maintenance of control systems after harvest, diverse distribution items, support of funds to construct the cyber market, direct transaction funds, and logistics facilities funds had relatively low priority.

SSM과 중소상인 상생 협력 방안의 연구

류인철,강한수,최용석 한국지역발전학회 2012 지역발전연구 Vol.12 No.1

본 연구는 최근 우리나라의 SSM 출점실태 파악과 2010년 국회에서 통과된 SSM 개정법을 검토하여 상생협력 방안을 제시하였는데 다음과 같다. 첫째, 법률적 보호 장치마련이 우선적으로 이루어져야 한다. 현재 일선 자치단 체와 대형마트간의 영업규제를 둘러싼 대립이 뚜렷한 해결책을 찾지 못하자 법 으로 이를 강제하려는 움직임이 현실화되고 있다. 상위 법률이 강화되고, 일선 자치단체들의 관련 조례도 법률적 허점을 보완한다면 대형마트 등의 영업시간 제한은 법률적으로 가능해진다. 둘째, 지역상권의 자구노력이 필요하다. 지역상권의 자구노력 방안으로는 시설 의 현대화 지원, 소비자와 호흡하는 서비스 창출, 차별화 정책, 상인들의 의식개 선과 더불어 지역주민들의 의식변화도 필요, 경쟁력 제고를 위한 상인의 역할과 의지, 전통시장 미를 살리는 것 등이다. 셋째, 대형마트의 자발적 휴무가 필요하다. 법과 조례 등 강제성으로 휴무를 하 는 것보다 대형마트의 자발적인 휴무가 필요하다. 넷째, 대형마트가 지역사회에 공헌해야 한다. 수백억대의 연간매출을 올리는 대형마트 가 지역사회에 장학사업, 사회봉사사업, 지역 문화예술지원 사업, 지역상 품 판로확대 사업 등의 방법으로 공헌해야 한다. 다섯째, 대형마트의 사회적 책임 수용이 필요하다. 일선 자치단체와 사회단체 등을 중심으로 대형마트를 대상으로 지역주민 정규직 고용, 지역 업체 입점, 지 역 중소기업 제품구매, 현금 매출액 지역은행 예치, 대형마트 현지법인화, 연간 총매출의 일정액 지역 환원, 지역상권 활성화 부담금 강제부과 등 상생방안이 논의되고 있으나, 이를 법률적으로 시행하기는 어려운데, 결국, 대형마트 등이 사회적 책임 차원에서 이를 수용하지 않으면 실행은 불가능하다. 여섯째, 현지 법인화가 추진되어야 한다. 법인화가 추진되면 기업경영에 필요한 모든 요소를 독립적으로 수행하기 때문에 인재채용, 협력업체 육성, 지역상품 판로확대 면에서 지역에 기여하는 바가 클 수밖에 없다. 또한 지역자금의 역외 유출 방지와 지역 농수산물 구입, 지역인재 채용 등으로 지역경제 활성화에 큰 도움이 될 것이며, 지역자금의 역외유출 방지와 지역 농수산물 구입, 지역인재 채용 등으로 지역경제 활성화에 큰 도움이 될 것이다. 이상과 같이 현재 대형마트와 재래시장 사이의 갈등은 끝이 보이지 않는다. 대 형마트 규제가 속속 법제화되고 있지만, 대형마트측은 법원소송까지 제기하며 거세게 반발하고 있는 형국이다. 재래시장과 소상공인들은 신규입점 및 영업시 간 규제에도 불구하고, 추가 규제를 요구하고 있다. 과연 대형마트와 중소상인과 상생 협력하는 방법은 규제보다는 서로 공생할 수 있는 방안을 모색하는 것이 가장 중요하다.

Poly(alpha-hydroxy acids) 제제 생분해성 차폐막의 치주조직 재생유도능력에 관한 조직학적 장기관찰

류인철,구영,정종평,한수부,최상묵,Rhyu, In-Chul,Ku, Young,Chung, Chong-Pyoung,Han, Soo-Boo,Choi, Sang-Mook 대한치주과학회 1997 Journal of Periodontal & Implant Science Vol.27 No.3

The recent trend of research and development on guided tissue regeneration focuses on the biodegradable membranes, which eliminate the need for subsequent surgical removal. They have demonstrated significant and equivalent clinical improvements to the ePTFE membranes. This study evaluate guided tissue regeneration wound healing in surgically induced intrabony periodontal defects following surgical treatment with a synthetic biodegradable membranes, made from a copolymer of glycolide and lactide, in 8 beagle dogs. After full thickeness flap reflection, exposed buccal bone of maxillary and mandibular canine and premolar was removed surgically mesiodistally and occlusoapically at $6mm{\times}6mm$ in size for preparation of periodontal defects. In experimental sites a customized barrier was formed and fitted to cover the defect. Flap was replaced slightly coronal to CEJ and sutured. Plaque control program was initiated and maintained until completion of the study. In 4, 8, 16 and 24 weeks after surgery, the animals were sacrificed and then undecalcified specimens were prepared for histologic evaluation. Histologic examination indicated significant periodontal regeneration characterized by new connective tissue attachment, cementum formation and bone formation. These membranes showed good biocompatibility throughout experiodontal period. The barriers had been completely resorbed with no apparent adverse effect on periodontal wound healing at 24 weeks. These results implicated that present synthetic biodegradable membrane facilitated guided tissue regeneration in periodontal defect.

생약 추출물이 세포성장 및 cytokine 생산에 미치는 영향

류인철,손성희,정종평,배기환 충남대학교 약학대학 의약품개발연구소 1993 藥學論文集 Vol.9 No.-

The native connective tissue attachment of the periodontium is known to be a complex consisting of gingival fibroblasts, periodontal ligament cells, gingival epithelial cells, cementum, alveolar bone and extensive extracellular matrix (collagen, glycoprotein and proteoglycans). The purpose of this study was to evaluate the effects of natural extracts on DNA, collagen and protein synthesis and inhibition of cytokine production in the gingival and periodontal ligament fibroblasts and gingival epithelial cells. Healthy gingibal tissue was obtained from orthodontic treatment patients, and gingival epithelial cells, gingival fibroblasts and periodontal ligament cells were isolated and cultured from the samples. After treated with Ginseng protein, Pluronic F-68, Scutellariae Radix, centella asiatica, PDGF, IGF, DNA synthesis, total protein and collagen synthesis, and cytokine production of gingival epithelial cell, gingival fibroblast and periodontal ligament cells were measured. MTT method for DNA synthesis, Peterkofsky and Dingerman method for total protein and collagen synthesis, and IL-1 ELISA kit for cytokine production were used. The proliferation of epithelial cells was enhanced in Centella asiatica, Ginseng protein, Pluronic F-68 and Scutellariae Radix. The activities of PDL cells were increased in PDGF, IGF, and Pluronic F-68. Higher collagen synthesis was observed in Scutellariae Radix and total protein synthesis was increased in Scutellariae Radix and PDGF. The inhibitory effects on IL-1, IL-6, TNF-α were observed in all exrracts.

홍화 추출물이 치주인대세포, 조골세포 활성도에 미치는 영향

류인철,이용무,구영,배기환,정종평,Rhyu, In-Chul,Lee, Yong-Moo,Ku, Young,Bae, Ki-Whan,Chung, Chong-Pyoung 대한치주과학회 1997 Journal of Periodontal & Implant Science Vol.27 No.4

Safflower(Carthamus tinctorius $Linn\acute{e}$ has been traditionally used for the treatment of blood stasis, and Dipsasi Radix has been used as a drug for fracture in Chinese medicine. The purpose of present study was to examine the biologic effects of safflower extract and Disasi radix extracts on the periodontal. ligament cells and osteoblastic cells and on the wound healing of rat calvarial defect. The ethanolic extract of safflower blossom, safflower seed and Dipsasi Radix(125, 250, and 500 ${\mu}g/ml$) were prepared as test group, and PDGF-BB(lOng/ml) and unsafonifiable fraction of Zea Mays L.(125, 250, and 500 ${\mu}g/ml$) were employed as positive control. The effects of each agents on the growth and survival, ALPase activity, expression of PDGF-BB receptor, chemotactic response of PDL cell and ATCC human osteosarcoma MG63 cells in vitro were examined. The tissue regenerative effect of each extracts was evaluated by histomorphometric measuring of newly formed bone on the 8mm defect in rat calvaria after oral administration of 3 different dosages groups : 0.02, 0.1 and 0.35g/kg, per day. It was also employed the same dosages of unsaponifiable fraction of Zea Mays L. as positive controls. Safflower blossom extract, safflower seed extract, and Dipsasi Radix extract stimulate the cellular activity of MG63 cells in concentration range of $125-500{\mu}g/ml$, and safflower bolssom extract and safflower seed extract stimulate also the cellular activity of periodontal ligament cells in concentration range of $250-500{\mu}g/ml$. In activity of ALPase, $250-500{\mu}g/ml$ of safflower blossom extracts showed significant stimulating effects on MG63 cells, and the same concentration range of safflower seed extracts showed significant effect on periodontal ligament cells. In the recovery on PDGF-BB receptor expression which was depressed by $IL-1{\beta}$, $125-250{\mu}g/ml$ of safflower blossom extracts and $250-500{\mu}g/ml$ of safflower seed extracts showed significant increasing effect on MG63 cells, and $500{\mu}g/ml$ of safflower blossom extract and $250-500{\mu}g/ml$ of safflower seed extracts showed significant effect on periodontal ligament cells. In chemotactic response, among all tested group, safflower seed extracts only were chemotactic to MG63 cells and periodontal ligament cells in concentration range of $125-500{\mu}g/ml$. Also in the view of bone regeneration in rat calvarial defect model, the only group that was orally administrated 0.35g/kg, day of safflower seed extract showed significant new bone formation. These results suggested that safflower extracts might have a potential possibilities as an useful drug for adjunct to treatment for regeneration of periodontal defect.

흡수성 차폐막의 치주조직 재생에 혈소판유래 성장인자가 미치는 영향

류인철,배규현,설양조,구영,이승진,한수부,최상묵,정종평,Rhyu, In-Chul,Bae, Kyoo-Hyun,Seol, Yang-Jo,Ku, Young,Lee, Seung-Jin,Han, Soo-Boo,Choi, Sang-Mook,Chung, Chong-Pyoung 대한치주과학회 1999 Journal of Periodontal & Implant Science Vol.29 No.3

The ultimate objective of periodontal treatment is to stop disease progression and to regenerate destroyed periodontal tissues and thereby regain normal function. Growth factors are naturally found polypetides which stimulate many cellular activities pertaining to wound healing by acting as signal molecule in controlling cell movement, proliferation, and matrix production. Platelet derived growth factor (PDGF) is 28,000-35,000 Da molecular weight dimeric protein with 2 long positively charged polypeptide chains connected by sulfide bonds. The purpose of this study is to evaluate histologically the initial guided tissue regeneration in a periodontal defect f a beagle dog treated with a biodegradable membrane formed with polylactic acid (poly-L-lactic acid) and polyglycolic acid loaded with 200ng/$cm^2$ platelet derived growth factor. 2 beagle dogs were used in he experiment. $5mm{\times}6mm$ alveolar bone defect was formed in upper and lower canines and third premolars and a reference notch was placed. PDGF-BB non-containing membrane was used as control. Each defect was randomly assigned to the test roup or the control group. The dogs were sacrificed 3 weeks after membrane placement. Toluidine blue and multiple staining was done for histological analysis. In the 3 week specimen in the control group, no new one formation could be seen. Small amount f bone resorption below the notch could be seen. In the notch, loose connective tissue with infiltration of inflammatory cells could be seen. Also thin discontinuous new cementum could be seen and the membrane still retained its structure. Where PDGF-BB containing membrane was used, new bone formation could be seen in the notch at weeks and also continuous thin cementum could be seen. PDL cells were observed between new bone and new cementum and some were attached to bone and cementum. These results suggest that new bone and cementum formation seen when PDGF-BB loaded membrane was used was due to inhibition of downgrowth of epithelial cells and also due to continuous release of the growth factor. Further study on the resorption characteristics of the membrane nd the release characteristics of the PDGF-BB is necessary. Also, development of a membrane easier to use clinically is necessary.