Reconstruction of Rabbit Corneal Epithelium on Lyophilized Amniotic Membrane Using the Tilting Dynamic Culture Method

Ahn, Jae-Il,Lee, Doo-Hoon,Ryu, Yang-Hwan,Jang, In-Keun,Yoon, Mun-Young,Shin, Youn Ho,Seo, Young-Kwon,Yoon, Hee-Hoon,Kim, Jae-Chan,Song, Kye-Yong,Yang, Eun-Kyung,Kim, Ki-Ho,Park, Jung-Keug Blackwell Publishing Inc 2007 Artificial Organs Vol.31 No.9

<P>Abstract: </P><P>Rabbit corneal epithelium was reconstructed using tilting dynamic culture with a self-manufactured, amniotic membrane (AM) supporter and a lyophilized amniotic membrane (LAM). Rabbit corneal epithelial (RCE) cells were cultured and cryopreserved after isolation from the limbus. The second- and third-passage RCE cells were plated onto the epithelial side of the LAM of Ahn's AM supporter. Two days later, the air–liquid interface culture was maintained with third-passage RCE cells for 6 days and second-passage corneal epithelial cells for 9 days. The average viability of thawed RCE cells, assessed using trypan blue dye exclusion, was 77.42%. The reconstructed corneal epithelium was characterized by histological (hematoxylin and eosin) and immunohistochemical staining (proliferating cell nuclear antigen) for light microscopy, and by reverse transcriptase-polymerase chain reaction, glucose assay, and transmission electron microscopy. The basal layer of the reconstructed corneal epithelium was well formed, and the epithelium was tightly constructed due to the increase in cell proliferation and differentiation caused by the tilting dynamic culture, as opposed to static culture. Tilting dynamic culture was useful for the reconstruction of the epithelium using easily damaged epithelial cells and resulted in more stratum cell layers. Moreover, cytokeratin (CK3) mRNA expression in tilting dynamic cultured third-passage RCE cells seeded onto AM was greater than in static cultured third-passage RCE cells. The morphology of the reconstructed corneal epithelium on LAM by tilting dynamic culture for 9 days resembled that of the skin epidermis. This was thought to be because the tilting dynamic culture not only accelerated the proliferation and differentiation of cells by physical or mechanical stimulation, but also ensured that the supply of medium was delivered to the basal cells more efficiently. Thus, the reconstruction of the corneal epithelium using LAM and tilting dynamic culture was considered to be a good in vitro model for autologous or allogeneic transplantation of corneal epithelium and skin epidermis in patients with damaged epithelia. </P>

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1994)

김재홍,안진균,정성재,김영태,김중환,김시영,이석종,이홍렬,서호석,김경훈,권혁진,정우권,고우석,이용석,안필수,오준규,오용섭 대한화학요법학회 1996 대한화학요법학회지 Vol.14 No.1

Background : In recent years gonorrhoea has been panendemic and remains one of the most common Sexually Transmitted Diseases in the world, especially in developing countries. Objective & Method : For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria Gonorrhoeae(PPNG), Ive have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results : In 1994, 168 strains of N. gonorrhoeae were isolated, among which 109 (64,9% ) were PPNG. Conclustion : Our results suggests that the prevalence of PPNG in Seoul is still increasing.

장거리 달리기 선수에서 횡문근융해와 생화학적 지표의 변화에 대한 연구

안선호,최석채,김태현,나용호,송주흥,이영진,조지현,최석준 대한신장학회 2000 대한신장학회잡지 Vol.19 No.5

배 ‘신고’에 GA3, A3+4+7 및 GA4+7 처리가 과실 형태 및 품질에 미치는 영향

이욱용, 최진호, 천종필, 안영직 배재대학교 자연과학연구소 2017 自然科學論文集 Vol.28 No.1

우리나라에서 많이 사용되고 있는 숙기촉진용 식물생장조절제의 성분 차이에 따른 과실의 형태적 기형 및 품질에 미치는 영향을 조사하였다. 수확기에 과실 무게를 측정한 결과, GA3, GA3+GA4+7 및 GA4+7의 모든 제제에서 과실비대가 유의하게 나타났으며 GA4+7 단용 제제가 가장 높은 비대율을 보였다. 과실의 형태적 이상은 종자생장과 관련이 있는데 GA4+7 제제를 사용한 과실에서 비정상종자발생 비율이 가장 높았다. 저온저장 후 과실의 품질변화에서 GA4+7 제제의 감모율이 가장 높았으며 과육경도의 경우, GA4+7 제제가 GA3+GA4+7 사용 과실에 비해 낮게 나타났다. 이상의 결과를 고려하면 GA4+7의 단용처리구의 경우 과실의 비대효과는 크지만 수확기 이상비대 과실의 출현, 저장 중 경도 감소 등 전반적인 과실품위 유지가 어려울 것으로 판단되므로 지베렐린 이용 시 보다 주의가 필요하다고 판단된다. In this study, we tested the effects of registered or unregistered plant growth regulator gibberellin pastes on fruit shape and internal in 'Niitaka' pears. The treatment of the GA4+7 produced the most enlarged fruit and the fruits treated with unregistered GA paste showed lowest flesh weights among the treatments. Fruit shape of 'Niitaka' pears was closely associated with the formation of normal seeds. And the fruits treated with unregistered GA had the highest proportion of abnormal seed with severe occurrence of abnormal-shaped fruit. We also compared the quality indices after cold storage. The highest fruit weight loss and the lowest flesh firmness values were found in the fruits treated with unregistered GA treatment. Futhermore, the use of unregistered GA products possibly smuggled from other country may occur irregular fruit development through the deficiency of seed development. Consequently, it is recommended that the use of registered GA products such as mixture of GA3 and GA4+7 intensely important to the conformation of high quality fruit production in 'Niitaka' pears.

소화성 궤양에 대한 Nizatidine의 임상효과

안정기,이기천,허승식,이종선,정현용,이헌영,김영건 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.1

Nizatidine is a newly introduced potent H2-receptor antagonist of acid secretion with long duration of action. For evaluation of clinical efficancy and safety, twenty one patients with endoscopically proved thirteen gastric ulcers and eight duodenal ulcers were treated with nizatidine 300mg at bed time via per os for 6 weeks. The results were as follows 1. The rate of complete endoscopic healing of duodenal ulcer 92.3% and 87.5% after 6 weeks. The overall healing rate of peptic ulcer was achieved in 89.9% at 6 weeks. 2. Clinical symptoms disappeared in 84.6%, 92.3% and 100% of patients with duodenal ulcer and 75.0%, 87.5% and 87.5% patients with gastric ulcer at 2, 4 and 6 weeks respectively. 3. There were not developed any symptoms and signs suggesting side effect of drug. In conclusion, data from this study suggest that nizatidine 300mg p.o. at sleeping time is effective and safe in treatment of peptic ulcer disease, and is well tolerated on a short-term basis.

65세 이상의 노령층에서 고지혈증의 위험요인

김용배,장원기,황보영,김화성,안규동,이병국,이용진,김세훈,김승수,김승훈,김영록,김웅빈,이성수 순천향의학연구소 2006 Journal of Soonchunhyang Medical Science Vol.12 No.1

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Morphology를 이용한 Range 영상의 Edge 검출 및 분류

안도건,박용기,김두영 동아대학교 공과대학 부설 한국자원개발연구소 1995 硏究報告 Vol.19 No.1

Range images provide an explicit encoding of the shape and geometric structure of the objects in the image from the point of view of the sensor. Since morphological methods are inherently geometric in nature, they are ideally suited for the analysis of range images. In this paper we propose the method for edge detection and classification in range images using morphological method and surface normals. Dilation residues and erosion residues of structuring elements at different scales are used to extract edges and surface normals are used to remove noises.

자발성 기흉을 동반한 폐림프관평활근종증 1예

안용환,정종훈,김소영,김휘정,김학렬,양세훈,최순호,문형배,조향정,정은택 圓光大學校 醫科學硏究所 2005 圓光醫科學 Vol.20 No.1

연노출 남자 근로자들의 간기능 지표에 관한 연구

황보영,김용배,리갑수,이성수,안규동,이병국 大韓産業醫學會 2002 대한직업환경의학회지 Vol.14 No.3

목적 : 연취급 근로자들에서 연노출이 간기능에 미치는 영향을 알아보기 위하여 시도되었다. 방법 : 모 1차 제련업체에서 연을 취급하는 남자 근로자들 중 B형 간염 항원 보균자 및 간장질환 경험이 있는 근로자를 제외한 274명을 대상으로 하였고, 대조군은 B형 간염 항원 및 간장질환 경험이 없으면서 과거 직업력상 연을 취급하는 직장에 근무한 적이 없는 제조업체 생산직 근로자 113명을 대상으로 하였다. 연노출의 지표로서는 혈중 연량, 혈중 zinc protoporhourin, 요중 δ-aminolevulinic acid 등을 측정하였고, 간기능의 지표로서 aspartate aminotransferase(SGOT), alanine aminotransferase(SGPT), γ-glutamyltransferase(γ-GTP)를 측정하였며, 또한 간기능에 영향을 줄 수 있는 혼란변수로서 연령, 흡연량(pack-year), 음주량(주간 평균 마신 술을 2홉들이 소주병수로 환산), 체질량지수(BMI) 및 연 노출기간을 조사하였다. 간기능 지표(SGOT, SGPT, γ-GTP)의 결과를 각각의 기준치(reference value)에 의거 정상과 비정상으로 판정한 후, 각 비정상 결과에 대한 각 변수들의 영향을 로지스틱 회귀분석하였고, 또한 간기능 지표(SGOT, SGPT, γ-GTP)에 연노출 지표가 미치는 영향을 확인하기 위하여 혈중 연 농도, 혈중 ZPP, 요중 δ-ALA를 각각 독립변수로 하여 다중회귀분석을 실시하였으며, 혼란변수로서 연령, 체질량지수, 흡연량 및 음주량, 연 노출기간 등이 포함되었다. 최종모델은 동일한 혼란변수를 포함하도록 하였다. 결과 : 비정상 SGOT(>35 IU/L)에 대한 로지스틱 회귀분석 결과, 혈중연 농도의 비차비는 1.04(95 % CI, 1.008-1.066)였으며, 음주량(비차비 1.28 : 95 % CI, 1.054-1.549)과 체질량지수(비차비 1.24: 95 % CI, 1.075-1.421) 역시 유의한 변수로 나타났다. SGOT를 종속변수로 하고 연령, 체질량지수(BMI), 연노출 근무기간 그리고 흡연량 및 음주량을 통제한 중회귀분석에서 혈중 연농도는 SGOT를 유의하게 증가시키는 것으로 나타났다(p<0.05). 결론 : 연은 연노출 남자 근로자의 간기능 지표 중 SGOT에 유의한 영향을 미칠 수 있는 것으로 나타났다. Objectives: To evaluate the effects of lead on the liver function indices in male lead wokers. Methods: The study comprised of 274 lead workers and 113 non-lead workers who were confirmed not to have HBsAg (Hepatitis B surface Antigen). Blood lead (PbB), blood zinc protoporphrin (ZPP) and urinary δ-aminolevulinic acid (ALAU) were selected as parameters for monitoring exposure to lead while aspartate aminotransferase (SGOT), alanine aminotransferase (SGPT) and γ-glutamyltransferase (γ-GTP) were chosen as parameters for monitoring liver function. Demographic information, such as age, smoking and drinking histories were collected. The duration of work for the lead workers, and their BMI(body mass index), were also obtained. Result: After adjusting for possible confounders {age, BMI, work duration, smoking (pack-year) and drinking (bottles of Soju consumed per week)}, blood lead was observed to be a signficant variable from the logistic regression analysis of the abnormalities of SGOT(>35 IU/L), with an odds ratio of 1.04 (95% CI, 1.008-1.066). The amount of alcohol drunk (odds ratio 1.28: 95% CI, 1.054-1.549) and BMI (odds ratio 1.24: 95% CI, 1.075-1.421) were also aignificant variables. From the multiple linear regression analyses, blood lead was signficantly, and positively, associated with SGOT (p<0.05). Conclusions : These findings suggest that exposure to lead may have an effect on SGOT in male lead workers.

외상성 횡격막 손상

안성국,이상목,이기형,고석환,김용호,박호철,고영관,조규석 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

Fifty two cases of traumatic diaphragmatic injuries that we have experienced from Jan. 1973 to Oct. 1994 were evaluated. The following results were obtained. The age of the patient was ranged from 1 to 74 years. Male was 38 and female was 14 in number with a ratio of 2.7 : 1. The traumatic diaphragmatic injuries were due to blunt trauma in 35 cases(motor vehicle accident 25, fall down 8, press 1, kick by fight 1) and penetrating trauma in 17 cases(stab wound 15, shot wound 1, explosion 1). In the blunt trauma, the preoperative diagnosis of the diaphragmatic injury was possible in 25 out of 35 cases(71%) and in the penetrating trauma, 15 out of 17 cases(88%). In the blunt trauma, the repture site was located in the left in 22 cases(63%) and in the right in 13 cases(31%). In the penetrating trauma, the rupture site was located in the left in 9 cases(53%) and in the right in 8 cases(47%). In the blunt trauma, 20 cases(63%) were treated within 24 hours and in the penetrating, 15 cases(88%) within 24 hours. In the blunt trauma, the herniated organs into the thorax were stomach(7), omentum(6), spleen(6), liver(5), colon(4), small bowel(2) and in the penetrating, stomach(7), colon(6), omentum(3), liver(2), and spleen(1) were herniated. Injury severity score(ISS) of 35-blunt trauma ranged from 11 to 66 with mean value of 30.6. Mean ISS of survivors and nonsurvivors was 27.6 and 52.7 respectively. The diaphragmatic repair of 49 cases was performed with thoracic approach in 23 cases, thoracoabdominal approach in 7 cases and abdominal approach in 19 cases, and 3 cases were not operated. The postoperative complication and mortality were developed in 16 out of 49 cases(33%) and in 5 cases(9.6%) respectively, and the causes of death were hypovolemic shock(1), combined head injury(2), asphyxia(1), and pulomnary edema and renal failure(1). In conclusion, the injuries of the diaphragm should be suspected in all patients with severe blunt trauma or penetrating injuries at thorax and upper abdominal area near the diaphragm. All of the cases had associated injury and most of deaths were related to the severity of associated injuries.