심근경색의 위험인자로서 Thrombomodulin유전자변이의 의의

박현영,김영미,권혁문,지선하,조승연,정남식,심원흠,장양수 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.6

급성 관동맥증후군 환자의 신기능 이상과 주요 심장사건과의 관계

이상재,윤경호,최준호,송미진,김연경,박은미,김현정,이은미,유남진,김남호,오석규,정진원 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

한국비서자격시험의 개선점과 비교연구

남자현,이미정,이미영,이아영,정해금,최유정,이미숙,전인선,정혜림 이화여자대학교 법정대학 비서학과 1997 비서학연구 Vol.- No.17

동결온도가 해동 쇠고기의 품질에 미치는 영향

남주현,송형익,김미숙,문윤희,정인철 한국식품영양학회 1998 韓國食品營養學會誌 Vol.11 No.5

동결온도가 해동우육의 품질에 미치는 영향을 검토하였다. 해동드립감량은 -3℃ 동결이 30일째에 3.4%, -20℃ 동결이 60일째에 1.8%로 높았으며, -3℃가 -20℃보다 드립량이 많았다. 가열감량은 water bath와 pan 가열 모두 저장초기보다 동결저장중에 현저하게 높았으나, 동결온도에 의한 차이는 없었다. 염용성 단백질의 추출성은 동결기간이 경과함에 따라 감소하였고, -20℃에서 동결한 것이 -3℃에서 동결한 것보다 추출성이 높았다. 수용성 단백질의 추출은 -3℃의 경우 동결기간에 따라 현저한 변화가 없었으나, -20℃에서 동결한 것은 동결기간에 따라 증가하는 경향을 보였다. 명도(L)는 동결초기가 높았고 적색도(a)는 동결기간에 따른 변화가 없었으며, 황색도(b)는 동결초기보다 동결중에 더 높았다. Myoglobin의 변성율은 -3℃의 경우 45일째, -20℃의 경우 15일째 각각 94.4%와 94.0%로 가장 높았다. 전단력가는 동결초기보다 저장기간이 경과함에 따라 높아졌으며, 근원섬유의 소편화도는 동결저장중 현저한 변화가 없었다. pH는 -3℃와 -20℃ 모두 30일째까지 높아지다가 그 이후 낮아지는 경향을 나타내었다. This study was carried out to investigate the effects of freezing temperature on quality of thawed beef loin. In case of thaw drip loss, the freezing of -3℃ and -20℃ were higest by 3.4% to 30 days and by 1.8% to 60 days, respectively. And the thaw drip loss of -3℃ freezing was more than -20℃ freezing. The cooking loss of water bath and pan boiling were increased significantly during freezing than the beginning of freezing, but were not different -3℃ and -20℃. The salt soluble protein extractability was decreased during freezing, the -20℃ freezing was higher than -3℃ freezing. The water soluble protein extractability of -3℃ freezing was not significant different during freezing storage, that freezed at -20℃ was increased during freezing. The "L" value of the beginning of freezing was higher than during freezing, the "a" value was not different during freezing, and the "b" value during freezing was higher than the beginning of feezing. The myoglobin denatured percentaged of the -3℃ and -20℃ freezing were highest by 94.4% to 45 days and by 94.0% to 15 days, respectively. The shear force value during freezing was higher than the beginning of freezing, the myofibrillar fragmentation index was not significant different during freezing. The pH was increased to freezing 30 days, after that was decreased.

FGF-mediated FGFR signaling 이 두개봉합부의 초기형태발생 및 유지기전에 미치는 영향

남순현,김영진,서경환,김현정,박미현,유현모 大韓小兒齒科學會 1999 大韓小兒齒科學會誌 Vol.26 No.4

두개봉합부의 조기융합으로 알려진 Craniosynostosis는 두개봉합부 주위 조직들 사이의 조화로운 상호 작용이 파괴되었을 때 야기될 수 있다. 흥미롭게도 FGF receptor들, 특히 FGFR2의 point mutation은 여러 가지 형태의 craniosynostosis 증후군과 연관되어 있어, FGFR가 두개봉합부를 포함한 두개골 성장 발달과정에 중요한 유전자임을 시사하고 있다. Mouse 두개봉합부의 초기형태발생시 FGFR 유전자들의 기능을 알아보기위해, in situ hybridization 방법을 이용하여 FGFR2(BEK) 및 골아세포분화의 초기표지자인 osteopontin이, 태생기(E15-18)에서 출생후(P1-P3)까지, 두개골의 시상봉합부에서의 발현양상을 조사하였다. FGFR2(BEK)은 osteogenic front에 강하게 발현되었으며, osteopontin은 parietal bone의 exo-, endocranial부위에서 발현되었으나, parietal bone의 성장가장자리인 osteogenic front에서는 관찰되지 않았다. 두개봉합부에서의 FGF-mediated FGFR signaling의 역할을 좀더 심도깊게 조사 하기위해 E15.5 mouse의 두개골을 이용하여 in vitro 실험을 시행하였다. 흥미롭게도 osteogenic fronts 및 시상봉합부의 간엽조직 중앙에 FGF2 - soaked beads를 점적하여 36시간 기관배양한 결과, bead주위 조직들의 두께 및 세포수가 증가되었으며, osteogenic fronts 상에 FGF4 beads를 올려놓은 경우, 시상두개봉합부 중앙에 점적된 FGF4 beads나 BSA control beads에 비해, 골성장이 촉진되어 시상두개봉합부의 부분적인 소멸을 관찰할 수 있었다. 이와 더불어 FGFR2 beads는 osteopotin 및 Msxl 유전자의 발현을 유도하였다. 이 결과들을 종합해 볼 때, FGF - mediated FGFR signaling이 발육중인 두개골과 두개봉합부에서 세포의 증식과 분화의 균형을 조절하는데 중요한 담당하고 있음을 시사해주고 있으며, 이 과정중 FGF signaling이 osteopontin 및 Msxl 유전자의 발현을 조절하므로써 막내골 성장 및 두개봉합부의 유지기전에 기여할 것으로 사료된다. Craniosynostosis, the premature fusion of cranial sutures, presumably involves disturbance of the interactions between different tissues within the cranial sutures. Interestingly, point mutaions in the genes encoding for the fibroblast growth factor receptors(FGFRs), especially FGFR2, cause various types of human craniosynostosis syndromes. To elucidate the function of these genes in the early morphogenesis of mouse cranial sutures, we first analyzed by in situ hybridization the expression of FGFR2(BEX) and osteopontin, an early marker of osteogenic differentiation, in the sagittal suture of calvaria during embryonic (E15-E18) and postnatal stage (P1 - P3). FGFR2(BEK) was intensely expressed in the osteogenic fronts, whose cells undergo differentiation into osteoprogenitor cells that ultimately lay down the bone matrix. Osteopontin was expressed throughout the parietal bones excluding the osteogenic fronts, the periphery of the parietal bones. To further examine the role of FGF-mediated FGFR signaling in cranial suture, we did in vitro experiments in E15.5 mouse calvarial explants. Interestingly, implantation of FGF2 soaked beads onto both the osteogenic fronts and mid-mesenchyme of sagittal suture after 36 hours organ culture resulted in the increase of the tissue thickness and cell number around FGF2 beads, moreover FGF4-soaked beads implanted onto the osteogenic fronts stimulated suture closure due to an accelerated bone growth, compared to FGF4 beads placed onto mid-mesenchyme of sagittal suture and BSA control beads. In addition FGF2 induced the ectopic expression of osteopontin and Msxl genes. Taken together, these data indicate that FGF-mediated FGFR signaling has a important role in regulating the cranial bone growth and maintenance of cranial suture, and suggest that FGF-mediated FGFR signaling is involved in regulating the balance between the cell proliferation and differentiation through inducing the expression of osteopontin and Msxl genes.

여천공단지역의 유해 대기 오염물질에 대한 확률론적 위해도 분석

남병현,윤미정,이진홍 忠南大學校 環境問題硏究所 1998 環境硏究 Vol.16 No.-

The study centers on a probabilistic risk assessment for the inhalation of volatile organic compounds(VOCs) and toxic metals in Yeochon industrial complex. From the assessment, the 50th∼95th percentile cancer risks by VOCs for men and women are estimated to be ??∼??, ??∼??, respectively. Those percentile risks are higher than a de minimis risk of even ??. About 4% and 1% of the 95th and 50th percentile risks for both men and women come from the only human carcinogen, benzene, while about 85% and 96% come from a probable human carcinogen, 1,3-butadiene. The 50th∼95th percentile cancer risks by toxic metals for men and women are estimated to be ??∼ ??, ??∼??, respectively. Even 95th percentile risk is lower than a de minimis risk of 10-4. About 5% and 1% of the 95th and 50th percentile risks by VOCs and toxic metals for both men and women are due to heavy metals, respectively. A comparison of the probabilistic risk estimates with point risk estimate shows that the probabilistic analysis can provide a more complete assessment of risk and more useful information to risk manager and the public. However, uncertainty remains and improved Korean probabilistic density functions(PDF) are needed for factors such as airborne concentrations, inhalation rate, exposure frequencies, and cancer potency factors.

마우스에서 조합시험법에 의한 고려홍삼의 생식발생독성 평가

김미라, 박동선, 장자영, 신선희, 이예은, 백인정, 연정민, 이세라 , 남상섭, 황방연, 노재섭, 김순선, 조대현, 남상윤, 이범준, 김윤배, 윤영원 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.1

본문참조

一部 中· 高等學生의 健康管理에 관한 調査 硏究

김미성,남철현 慶山大學校 保健福祉硏究所 1999 保健福祉硏究 Vol.2 No.-

The following is the conclusion that I investigated 1,080 middle and high school students of Taegu city and Kyungsang-bukdo for one month, from may 2 to may 31 in 1996, for the purpose of helping to develop a program for school health education in the future by grasping students' health awareness and management, and analyzing the problems of affect this. 1. The general characteristics of the subjet, as follows The 50.0% of them is girls. The 33.3% is in the third grade. The 65.3% thinks their body-built average. The 79.1% thinks that their exonomic situation is average. The 90.0% has both parents. The 35.2% is buddhists, and 34.9% unbelievers 2. In self-judgement, the 65.0% thinks their health average, 25.8% good, and the rest 9.2% not so good. 3. The 86.6% answered that health is important thing, and by characteristics, the rate of answer is higher in small-medium cities, in the third grade students of vacational high schools, in bad economic situation, in students with both parents, in buddhists than other groups. respectively. 4. The students who answered "Yes" to the question of usually having a way to keep healthy are more in the first grade students of middle schools, good economic situation than other groups. 5. The 85.6% feel tired in physical and mental health, which is more in large sities, the third grade students of girls' high schools, bad economic situation, homes with only father, than groups(P<0.01). The variable of parents and religion is exclusive. 6. For nutritive condition, the 55.8% thinks it average, 34.1% good, 10.1% poor, and the last(poor nutrition) is higher in large cities, in the second grade students of vacational high schools, in bad economic situation, and in unbelievers than other groups. 7. For the attention of health idea, the 59.3% thinks it wanting, 30.9% much enough, 3.6% answered " a clean state", and the second, answering " much enough", is higher in small and medium cities, the first grade students of girls' middle school, good economicsituation, catholics with both parents than other groups. 8. For the levels of health knowledge, the 67.4% thinks it average, 28.4% answered "I think it wanting", which is higher in small and mediumcities, the third grade students of girls' high schools, bad economic situation, homes with only mother, unbelievers than other groups. 9. The 86.9% is non-smokers, the 7.3% sometimes smokes, the 5.8% ofter, and the last one (often smokes) is higher in farming and fishing villages, the second grade students of vocational high schools, bad economic situation homes with only father, Catholics than other groups(P<0.01). Excepting the variables of region and religion. 10. For the experience of taking a hallucinogen such as a bond or habit-forming medicines, the 97.8% answered "No" and the rest 2.2%"Yes". 11. For washing hands before meal, the 71.2% answered "Always", the 26.1% "sometimes", the 2.7%"No" and the last is higher in farming and fishing villages, the second grade students of vocational high schools, bad economic situation, homes with only father, catholics than other groups. 12. For the importance of health education, the 70.3% answered "necessary" and the 6.4% "unnecessary". For the idea of selecting a health teacher, the 55.1% answered "a nursing teacher", the 19.0%"all teacher", the 6.7% "an athletics teacher". 13. For the relation between variables, their good health situation is related to fatigue, nutrition, and eating dietary habit, and weariness is related to nutrition, sleep time, dietary habit, to the end, the nutrition is related to dietary habit, washing hands. As mentioned in the above, I reach the conclusion that related authorities and associations, schools, and all related teachers will have to pay positive and steady attention to the building of a system which of the aim is to cultivating nursing teacher into good health teachers, for developing a program of health education and practicing a school health education efficiently, so that middle and high school students, who are growing in physical and mental points, may enjoy their healthy and sound lives with desirable health knowledge

오매(Fructus Mume)의 항산화물질의 분리 및 특성연구

황현주,안은미,백남인,조재선,김해영 경희대학교 생명자원과학연구원 1998 硏究論文集 Vol.19 No.-

천연물로부터 새로운 항산화제를 개발하기 위한 연구의 일환으로 한방에서 사용되는 한약재 중의 하나인 오매(烏梅)의 물 추출물과 메탄을 추출물 및 순차 분획물에 대해 팜유, 우지와 돈지를 기질로하여 유지저아시험, Rancumat method, 전자공여작용 등으로 항산화성을 확인한 결과, EtOAc 분획물에서 강한 항산화 활성을 나타내어 column chromatography, thin layer chromatography를 통하여 활성성분에 관한 연구를 시도하였다. 1. 오매 용매분획물 중 EtOAc 분획물(400 ppm)은 유지저장실험(60℃)에서, 팜유, 우지와 돈지에 모두 우수한 효과를 나타내었으며, 특히 우지에서 높은 활성을 나타내었고, BHA(200 ppm)와 α-tocopherol(200 ppm)보다 활성이 높았다. 2. 오매 EtOAc 분획물(400 ppm)은 Rancimat method에서는 팜유, 우지와 돈지에 비교적 우수한 효과를 나타내었으며, 우지에서 AI가 4.39로 상당히 높은 활성을 나타내었다. 3. 오매의 용매분획물 중 EtOAc 분획물이 DPPH라디칼에 대한 강한 소거활성을 나타내었으며, 그 활성은 EtOAc 분획물의 농도에 의존하여 증가하였고, SC_(50)은 21.2μg/ml이었다. 4. 오매 EtOAc 분획물에서 분리한 8개 획분 중 E4에서 SC_(50)이 16.7μg/ml으로 높은 DPPH 라디칼 소거활성을 나타내었으며, 원인 물질은 여러 가지 스펙트럼 측정 결과 수종 물질이 혼합물인 것으로 밝혀져, 보다 순수한 형태로의 분리 후 정확한 동정의 시도가 요구되었다. Fructus Mume is the roasted hits of Prunus mwme and has been used as tradibonal Chmese medicine. The anboxidatwe effect of the extract from Fructus Mume was measured using different methodsn such as, electron donating abdihes(EDA), Rancimat and oven tests. The methanol extract obtained from the Fructus Mume was solvent-fractionated with ethyl acetate, n-butanol and water, respectively. The ethyl acetate fraction showed the highest antioxidative activity among them. To identify the compounds with antioxidative activity, the isolates from thm layer chromatography were studied further. This study demonstrates that Fructus Mume might be useful for the prevention or treatment of disorders associated with oxidative damage.

스토커형 저공해 소각로 설계를 위한 비반응 유동해석에 관한 연구

전영남,오현명,김미환 조선대학교 환경공해연구소 2000 環境公害硏究 Vol.16 No.1

The numerical simulation of non-reactive turbulent model and cold flow experiment were carried out to propose the incinerator type for the domestic refuses and to investigate the design factor and operating conditions. The "standard stoker incinerator" proposed in this study was modified from central flow type with carrying out by material and numerical simulations repeatedly. The stoker has the characteristics of good mixing between refuse and hot combustion gas in primary combustion chamber and between unburned gas inflowing and secondary air jet in secondary chamber. Also there was no recirculation zone in secondary chamber so that mixing time was increased with higher residence time. Therefore this incinerator could be possible for domestic refuse to burn up with the low emission and high efficiency combustion. Parametric screening studies were achieved in standard stoker incinerator. The parameters are stoker type, secondary air inflow type, secondary air injection angles, secondary injection air flow rate and primary air inflow rate. The results are as follows; 1. In the same conditions with standard stoker incinerator, flow pattern was not almost changed by changing inclined stoker type. 2. The injection potion and angle of secondary air had an effect on flow field in secondary combustion chamber. 3. Flow pattern was not almost changed with the variation of the flow rate of primary and secondary air injection. The results of parametric studies said that the main factors for the design are the incinerator configuration of secondary combustion chamber and/or the position and angle of secondary air injection.