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식용 식물자원으로부터 활성물질의 탐색-XXIV.- 식용 식물 추출물의 항암 활성 -
백남인,곽호영,권병목,송명종,이진희,양혜정,김대근,안은미 한국생약학회 2007 생약학회지 Vol.38 No.4
screening of anti-cancer activity for the MeOH extracts of 163 natural sources, which were registered as edibleplants by Korea Food & Drug Administration, exhibited 9 extracts to have significant inhibitory effects on farnesyl-proteintransferase (FPTase) and phosphatase of regenerating liver-3 (PRL-3). In order to confirm the inhibitory activity of these activeextracts, the activity assay was repeated for some fractions obtained from the active extracts using Medium Pressure LiquidChromatography (MPLC). Some fractions of Carya illinoensis, Chlorella vulgaris, Panicum miliaceum, Perilla frutescens, Ros-marinus officinalis showed over 50% inhibitory activity on FPTase as well as those of Capsella bursa-pastoris, ,C. vulgaris, Coix lacrymajobi, Myristica fragrans, P. miliaceum, R. officinalis did over 50% inhibitory activity on PRL-3.Key words edible plant, anti-cancer, FPTase, STAT3, PRL-3
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알팔파(Medicago sativa L .)로부터 지표성분의 분리
백남인,안은미,방면호,김무성,한재택 한국국제농업개발학회 1998 韓國國際農業開發學會誌 Vol.10 No.2
1. 알팔파 MeOH 추출물의 n-BuOH 분획으로부터 silica gel column chromato- graphy를 반복하여 glycosyl diglyceride 화합물을 분리하였다. 2. 화합물의 화학구조를 NMR, IR 등의 기기분석 결과와 산 및 알칼리 가수분해 반응을 이용하여 (2S)-1-O-linolenoyl-2-O-linolenoyl-3-O-[α-D-galactopyranosyl (1→6)-β-D-galactopyranosyl]-sn-glycerol 로 결정하였다. 3. 구성지방산의 종류 및 조성은 알칼리 분해하고 methyl ester 화한 후 GC/MS를 이용하여 분석한 결과 linolenic acid (84.2%), linoleic acid (7.2%), palmitic acid (5.2%), oleic acid (2.2%), stearic acid (1.2%)으로 결정되었다. The MeOH extracts obtained from the whole plants of Medicago sativa L. were fractionated with solvent system of EtOAc, n-BuOH, and H₂O, successively. From the n-BuOH extract, a major component, a glycosyldiglyceride compound, was isolated through the repeated silica gel column chromatographs. On the basis of several spectral data and hydrolysis results, its chemical structure was determined to be (2S)-1-O-linolenoyl-2-O-linolenoyl-3-O-[α-D-galactopyranosyl (1→6)-β-D-galactopyranosyl]-sn-glycerol. The kind and composition of fatty acids in the glyceride were determined through alkaline hydrolysis, methyl esterification, and followed by GC/MS such as ; linolenic acid (84.2%), linoleic acid (7.2%), palmitic acid (5.2%), oleic acid (2.2%) and stearic acid (1.2%).
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식용 식물자원으로부터 활성물질의 탐색-IV. 달래(Allium monanthum Max.)로부터 Galactosyldiglyceridem의 분리
백남인,안은미,김해영,박영두,장영진,김세영 한국생명과학회 2001 생명과학회지 Vol.11 No.1
n-BuOH fraction obtained from MeOH extracts of Allium monanthum was applied to repeated silica gel column chromatographies to give a glycosylglyceride. The chemical structure of the compound was determined to be 1-O-linolenoyl-2-O-linolenoyl-3-O-$\beta$-D-galactopyranosyl-누-glycerol on the basis of NMR data and by the adaptation of chemical methods.
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홍화 ( Carthamus tinctorius L. ) 로부터 활성물질의 분리
백남인,김융희,안은미,방면호,남지연,권병목 ( Nam In Baek,Yung Hee Kim,Eun Mi Ahn,Myun Ho Bang,Ji Youn Nam,Byung Mok Kwon ) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.2
The MeOH extracts obtained from the flower petals of Carthamus tinctorius were solvent-fractionated with EtOAc, n-BuOH, and H₂O, successively. From the n-BuOH exact 2 flavonoid compounds were isolated through the repeated silica gel column chromatographies. From not only the results of physico-chemical data including HMBC but also the adaptation of acid hydrolysis, the chemical structures of the compounds were determined as 3-Ο-[β-D-glucopyranosyl (1→2) β-D-glucopyranosyl] kaempferol and 3-Ο-[α-L-rhamnopyranosyl (1→6) β-D-glucopyranosyl] kaempferol. The compounds exhibited IC_(50) values in Grab2-Shc activity to be 43 and 47 ㎍/㎖, respectively.
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