검정콩 종피 함유 안토시아닌의 최적 추출조건

정명근,황영선,이현진,최수산나,임정대,강성택,한원영,백인열,김현경 한국작물학회 2008 한국작물학회지 Vol.53 No.1

검정콩 품질평가의 한 기준이 되는 안토시아닌의 함량평가를 위한 분석기술 개발의 일환으로 검정콩 종피 함유 안토시아닌의 최적의 추출용매 및 추출방법을 확립하고, 주요재배 검정콩 종피 함유 안토시아닌의 함량을 정량적으로 평가한 결과를 요약하면 다음과 같다. 1. 검정콩 종피 함유 안토시아닌의 최적 추출용매를 검토한 결과 1% HCl - H2O 로부터 1% HCl - 80% MeOH용액까지 통계적 차이가 인정되지 않았으나, 산술적으로 1% HCl - 60% MeOH 용액으로 추출하였을 때 총 안토시아닌의 함량이 7.72 mg/g으로 가장 높은 양상을 나타내었으나, 추출용매에 메탄을 함량이 높아질수록 안토시아닌 피크의 분리도가 극히 불량한 결과를 나타내므로 종합적 견지에서 볼 때 1% HCl - 20% MeOH를 이용하여 안토시아닌을 추출하는 것이 가장 우수한 것으로 평가되었다. 2. 검정콩 종피 함유 안토시아닌의 최적 추출방법 및 추출시간을 검토한 결과 상온 12시간 추출이 가장 우수한 결과를 나타내었으며, 추출시간이 24시간 이상 지속될 경우 추출시간이 증대됨에 따라 안토시아닌의 함량이 소량 감소되는 양상을 확인할 수 있었다. 3. 국내외 주요 재배 검정콩 품종별 종피 함유 안토시아닌 색소의 함량을 비교한 결과 총 안토시아닌의 함량 범위는 1.58-10.62 mg/g으로 조사되었고, 조사된 품종 중 검정 올콩이 10.62 mg으로 가장 높은 양상을 나타내었으며, 가장 낮은 함량을 나타낸 흑청콩 대비 약 7배 높은 안토시아닌 함량을 나타내었다. Soybeans (Glycine max) with a black seed coat have been widely utilized as food source and as a medicinal herbs in Korea. The pigmentation in the seed coat of black soybean is due to accumulate anthocyanins in the epidermis palisade layer. The anthocyanin content and composition of the black soybean seed coat are considered as a standard. of quality evaluation of black soybean. The main objective of this study was to investigate the optimal condition for an extraction method of anthocyanins and compare anthocyanin quantity and composition within black soybean varieties and germplasms. In the test of extraction solvent, absorbance at 530 nm and Hunter's a value were increased as increasing the concentration of MeOH, but Hunter's Land b values were the exact opposite of absorbance and Hunter's a values. There was no significant difference for anthocyanin contents from 1% HCl - H2O to 1% HCl - 80% MeOH. In the aspects of anthocyanin contents and HPLC peak resolution, 1% HCl - 20% MeOH extraction solution was the most suitable solvent. Among the 5 kinds of extraction method using 1% HCl - 20% MeOH solution, the anthocyanin contents of room temperature extraction at 72 h was the highest among the methods. High extraction temperature, sonication and reflux method influenced on the decrease of anthocyanin contents because of breakdown of anthocyanins. There was no significant difference for extraction time between 12 h and 24 h. However, the optimal extraction condition were at room temperature for 12 h. The anthocyanin contents in seed coats of black soybean were determined on the basis of HPLC peak area at 530 nm. Ten black soybean varieties and germplasms were tested with optimal conditions founded in this study. On the basis of antocyanin component, these can be classified into three groups; C3G, C3G + D3G and C3G + D3G + Pt3G. The total anthocyanin content in seed coats ranged from 1.58 to 10.62 mg/g of seed. The total anthocyanin content of the variety "Geomjeongol" was about 7 times higher than that of variety "Heugchong". Information for extraction method and diversity in antocyanin of soybean seed coats can be used for future research for germplasm evaluation and development of high quality black soybean varieties.

녹차 함유 카테친 및 카페인 동시분석을 위한 최적 HPLC 분석 조건

정명근,이민석 한국작물학회 2008 한국작물학회지 Vol.53 No.2

고 기능성 유용 차나무 품종육성 및 녹차의 이용성 증진 연구의 기초 자료를 제공하기 위해 녹차 함유 catechin 화합물 및 caffeine 성분을 동시에 분석할 수 있고, 재현성 및 검출 감도가 증진된 최적의 HPLC 분석 조건을 검토한 결과를 요약하면 다음과 같다. 1. 녹차에 함유된 catechin 화합물 및 caffeine의 동시분석을 위한 최적 HPLC 분석 조건을 검토한 결과 컬럼은 YMC-pak ODS-AM 303(25 cm)컬럼을 이용하고, 검출파장은 280 nm, 컬럼온도를 30℃ , 분당유속은 1 mL, 이동상 용매로는 A용매로 0.1% 인산 - 증류수, B용매로 100% MeOH를 사용하여 농도구배 조건으로 분석하는 것이 가장 효율적 분석법으로 조사되었다. 2. 녹차에 함유된 catechin 화합물 및 caffeine을 동시에 분석할 수 있는 최적 HPLC분석조건의 재현성 검정을 실시한 결과 머무름 시간(Rt.)의 변이계수는 최대 1.23% 미만, peak area의 변이계수는 최대 5.13% 미만을 나타내어 확립된 녹차 함유 catechin 9종 화합물 및 caffeine의 HPLC 정량분석법은 재현성이 높은 분석법으로 확인되었고, 각 cafechin 화합물 및 caffeine 성분의 최소 검출한계는 최소 50ppb(0.050 ppm, 50 ng/mL)이하로 조사되어, 확립된 HPLC 분석법은 녹차의 작물학적 특성 평가용으로 활용하기에 전혀 무리가 없는 분석법으로 판단되었다. The health benefits associated with tea consumption have resulted in the wide inclusion of green tea extracts in botanical dietary supplements, which are widely consumed as adjuvants for complementary and alternative medicines. Tea contains polyphenols such as catechins or flavan-3-ols including (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECG), and (-)-epigallocatechin gallate (EGCG), as well as the alkaloid, caffeine. The contents of catechins and caffeine in green tea are considered as a standard of quality evaluation of green tea. Therefor, the purpose of this study was to investigate the most suitable HPLC condition for simultaneous determination of catechins and caffeine in green tea extracts. The efficient HPLC analytical condition of catechins and caffeine contained green tea extracts was developed. The gradient elution employed a 250~;mm~;~times~;4.6~;mm i.d. YMC-pak ODS-AM 303 column. The gradient system was used two mobile phases. A gradient elution was performed with mobile phase A, consisting of 0.1% aqueous phosphoric acid, and mobile phase B, comprising 100% MeOH, and delivered at a flow rate of 1 mL/min as follows: 0~25~;min , 80% A; 26~50~;min , 80~70% A; 51 min, 80% A. 51~55~;min , 80% A. The UV detection wavelength was set at 280 nm. The limit of detection (LOD) for catechins and caffeine standards were under 50 ng/mL.

검정콩 자엽녹색 색소의 분리.동정

정명근,임정대,강성택,김경호,한원영,백인열,신두철,김관수,박시형 한국작물학회 2006 한국작물학회 학술발표대회 논문집 Vol.2006 No.-

작약근의 오관평가와 관련된 성분 검정

정명근 한국작물학회 2005 한국작물학회지 Vol.50 No.S

3년생 의성작약을 대상으로 오관검사의 평가기준으로 작용하는 맛, 색택 및 냄새에 관여하는 유리당, 유리아미노산, 유기산 및 향기성분을 분석하였다. 유리당 성분은 sucrose, glucose및 fructose등으로 구성된 것을 확인할 수 있었으며, 유리아미노산은 ~gamma-aminoisobuturic acid, arginine, citrulline 등 총 18종이 존재하였다. 작약근에 포함된 유기산은 oxalic acid, citric acid및 malic acid등 총 5종이 함유되어 있었다. 작약의 관능적 품질평가 기준 중 하나인 냄새를 구성하는 물질로서 eugenol을 포함한 11종의 향기성분을 확인하였다. 결국 작약의 특이적인 향기는 여러 물질에서 기인한 복합적 냄새로 평가될 수 있다. 12개정 일본 약국방 해설서(赤 등, 1991)에는 우수한 품질의 작약은 맛의 경우 처음에는 달고, 끝 맛은 떫고, 신맛이 있어야 하며, 작약의 특이한 냄새를 강하게 나타내는 것이 좋다고 기재하여 관능적 품질평가의 기준을 분명히 하였다. 그러므로 관능적 품질평가를 객관성이 결여되기 쉬운 감각적이고 주관적인 방법으로 평가할 것이 아니라 첨단 화학분석기기를 이용한 체계적 분석에 의하여 과학적이고 객관적이며 수치화된 평가방법을 적용하여 분석할 필요가 있을 것으로 판단된다. Experiment was conducted to establish the standard of quality evaluation in Korean cultivated peony roots. free sugars, free amino acids, organic acids and aromatic components, which were generally considered to be information components of five sensory test in peony roots, were examined. As free sugars, sucrose, glucose and fructose were identified in peony roots. ~gamma-aminoisobuturic acid, arginine and other 16 kinds of free amino acids were found in peony roots. The major organic acids of peony roots were oxalic acid, citric acid and malic acid. Eugenol and other 10 aromatic components were identified in peony roots by GC/MSD.

Optimal Extraction Conditions for Simultaneous Determination of Catechins and Caffeine in Green Tea Leaves

정명근,이민석 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.2

The amount of catechins and caffeine in green tea are considered a standard of quality evaluation for green tea. The objectives of this study were to investigate the optimal conditions for simultaneous extraction of catechins and caffeine and to compare their quantity and composition within the exotic tea varieties. Moreover, 8 tea varieties of exotic origin were tested with the optimal extraction conditions found in this study. Regarding the caffeine and catechins contents, a 2 h room temperature extraction using a 2% phosphoric acid-40% EtOH solution was the most suitable simultaneous extraction method. The total catechins and caffeine contents of the tea leaves ranged 44.25-64.80 and 9.26-13.11 mg/g, respectively. The optimal extraction method of catechins and caffeine in tea leaves may be used for further studies and breeding of high quality tea plants.

The relations of variable of nerve conduction studies to clinical symptoms scores in type 2 DM

정명근,원종철 대한내과학회 2018 대한내과학회 추계학술대회 Vol.2018 No.1

Microwave를 이용한 Tryptophan의 Decarboxylation

정명근,하기룡,백승욱 한국공업화학회 2009 한국공업화학회 연구논문 초록집 Vol.2009 No.1

급속 진행사구체신염에서의 가역적 후두부 뇌병증 증후군 1례

정명근,최윤미,김현정,김상현,박원도 대한내과학회 2018 대한내과학회 추계학술대회 Vol.2018 No.1

Construction of a Transcriptome-Driven Network at the Early Stage of Infection with Influenza A H1N1 in Human Lung Alveolar Epithelial Cells

정명근,조수영,이영식 한국응용약물학회 2018 Biomolecules & Therapeutics(구 응용약물학회지) Vol.26 No.3

We aimed to understand the molecular changes in host cells that accompany infection by the seasonal influenza A H1N1 virus because the initial response rapidly changes owing to the fact that the virus has a robust initial propagation phase. Human epithelial alveolar A549 cells were infected and total RNA was extracted at 30 min, 1 h, 2 h, 4 h, 8 h, 24 h, and 48 h post infection (h.p.i.). The differentially expressed host genes were clustered into two distinct sets of genes as the infection progressed over time. The patterns of expression were significantly different at the early stages of infection. One of the responses showed roles similar to those associated with the enrichment gene sets to known ‘gp120 pathway in HIV.’ This gene set contains genes known to play roles in preventing the progress of apoptosis, which infected cells undergo as a response to viral infection. The other gene set showed enrichment of ‘Drug Metabolism Enzymes (DMEs).’ The identification of two distinct gene sets indicates that the virus regulates the cell’s mechanisms to create a favorable environment for its stable replication and protection of gene metabolites within 8 h.

과적 차량 적발 동향 및 변인 상관 분석

정명근,오승민,이진우 대한교통학회 2022 대한교통학회 학술대회지 Vol.87 No.-