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골수이식 환자에서 거대세포바이러스감염 검출을 위한 항원혈증 검사와 Roche Amplicor?? CMV test의 비교
심상인,한경자,오은지,김병기,문연숙,박연준 가톨릭중앙의료원 가톨릭암센터 1998 암심포지움 Vol.- No.2
중합효소연쇄반응(PCR)과 CMV 항원혈증검사(CMV-Ag assay)는 비교적 간편한 방법으로 거대세포바이러스(Cytomegalovirus, CMV) 감염의 조기 진단을 위해 이용되고 있다. 본 연구에서는 Roche사의 Amplicor CMV test와 CMV-Ag assay를 비교하고 임상적 유용성을 알아보고자 하였다.
심상인,전우진,이지현 한국작물학회 2006 Korean journal of crop science Vol.51 No.4
Soybean quality is determined based on protein content, lipid content and fatty acid composition, and several functional components including isoflavones, anthocyanins and functional activity. Because the level of each component changes during seed development, it is necessary to know the concentration of quality-related components in developing seeds. Little is known of the pattern of changes in quality-related components. Seeds from field-grown soybean was harvest from the R6 stage to the R8 stage in 2004. Seed characteristics and the level of nutritional components were examined. Seed moisture content was dropped rapidly after the R7 stage in the tested varieties. Seed growth rate was the highest from the beginning of the R6 stage to the mid-R6 stage. Chlorophyll content was decreased rapidly in pods and seeds. However, seed growth period from the R6 to R8 was 35 days. The crude protein content was increased dramatically between 63 DAF and 70 DAF and then increased slightly. The pattern of isoflavone accumulation was nearly similar to that of seed weight increase. From the late R6 stage to the R7, the accumulation rate was higher as compared to other stages. The angiotensin inhibitory activity was increased according to seed development from 63 (R6) to 84 DAF (R8). The difference of inhibitory activity in heated soybean powder, however, was not great among stages. The inhibitory activity was affected by heating treatment. The most effective heating time was 10 min. Excessive heating longer than 30 min resulted in a lowered inhibitory activity of soybean on ACE.
유방암종의 p53, c-erbB-2및 nm23단백질 발현양상
심상인,한경자,김영신,강창석,이교영,김용구 가톨릭중앙의료원 가톨릭암센터 1996 암심포지움 Vol.- No.2
저자들은 유방암에서 p53, c-erbB-2와 nm23등의 단백질 발현실험을 통하여, 암의 진행과 전이에 관여하는 것으로 알려진 유전자들의 변화로 인한 단백질의 발현 및 억제가 유방암의 진행과 전이에 어떤 관련이 있으며 또한 이들 상호간에 어떤 연관성이 있는지에 대하여 알아보고자 하였다.
심상인 한국응용생명화학회 1993 한국응용생명화학회 학술발표회 Vol.1993 No.-
The environmental pollutions were a serious problem in Korea recently. So many researcher have studied the effect of environmental pollution on plants and agro-ecosystem, but the basic mechanisms of environmental stresses were various. One of the important mechanisms was oxidative stress caused by active toxic oxygen. The toxic oxygen was generated by several stresses, abnormal temperature, many xenobiotics, air pollutants, water stress, fugal toxin, etc. In the species of toxic oxygen which is primary inducer of oxidative stresses, superoxide, hydrogen peroxide, hydroxyl radical and singlet oxygen were representative species. The scavenging systems were divided into two groups. One was nonenzymatic system and the other enzymatic system. Antioxidants such as glutathione, ascorbic acid, and carotenoid, have the primary function in defense mechanisms. Enzymatic system divided into two groups; First, direct interaction with toxic oxygen(eg. superoxide dismutase). Second, participation in redox reaction to maintain the active antioxidant levels(eg. glutathione reductase, ascorbate peroxidase, etc.).
심상인,강병화 ( Sang In Shim,Byeung Hoa Kang ) 한국환경농학회 1993 한국환경농학회지 Vol.12 No.3
The environmental pollutions were a serious problem in Korea recently. So many researcher have studied the effect of environmental pollution on plants and agro-ecosystem, but the basic mechanisms of environmental stresses were various. One of the important mechanisms was oxidative stress caused by active toxic oxygen. The toxic oxygen was generated by several stresses, abnormal temperature, many xenobiotics, air pollutants, water stress, fugal toxin, etc. In the species of toxic oxygen which is primary inducer of oxidative stresses, superoxide, hydrogen peroxide, hydroxyl radical and singlet oxygen were representative species. The scavenging systems were divided into two groups. One was nonenzymatic system and the other enzymatic system. Antioxidants such as glutathione, ascorbic acid, and carotenoid, have the primary function in defense mechanisms. Enzymatic system divided into two groups; First, direct interaction with toxic oxygen(eg. superoxide dismutase). Second, participation in redox reaction to maintain the active antioxidant levels(eg. glutathione reductase, ascorbate peroxidase, etc.).
Ecophysiology of Seed Germination in Chinese Milk Vetch (Astragalus sinicus L.)
심상인,강병화 한국작물학회 2004 Korean journal of crop science Vol.49 No.1
Germination and emergence habits of Chinese milk vetch (CMV) were examined to obtain the basic information for the effect of environmental conditions and cultural practices on the seedling density. Seed germination tests with different water potentials, temperatures, and soil and water depths showed the environmental effects on the characters related to seed germination in CMV. Imbibition under different temperatures reflects that initial velocity was rapid at higher temperature, however, the times to full imbibition were not different between 15℃ and 25℃. The optimal germination temperature for CMV germination was ranged from 15℃ to 20℃ and the germination was highly affected by water potential of media at relatively high temperature above 20℃. When the seeds were sown in flooded condition the germination was not proportionally affected by water depth. In addition, there was no correlation between water depth and oxygen concentration. The germination of seeds flooded by 2 cm water depth were poorly germinated compared to other depths. Results indicated that the germination of submerged seeds was more highly influenced by flooding depth more the temperature, it was also affected more strongly at 10℃ than 20℃. Emergence of CMV depending on the thickness of covered soil was poor when the soil layer was greater than 5 cm. In the experiment with seeds collected between 22 days after flowering (DAF) and 52 DAF, the highest germination ability of CMV seeds was observed at 39 DAF and germinability was decreased subsequently as seeds became mature. The lower germinability may be due to the enhanced seed dormancy.