간경변 환자에서 혈중 Lipoprotein (a)와 저밀도지단백 콜레스테롤 및 Apolipoprotein (a)의 상관관계

전사일,정영화,민원기 대한임상병리학회 2002 대한임상병리학회지 Vol.22 No.2

식사 및 검체 보관이 면역 측정법에 의한 저비중지단백 콜레스테롤 직접 측정법에 미치는 영향

임환섭,정재림,박광일,김정호,권오헌 대한임상병리학회 1999 대한임상병리학회지 Vol.19 No.1

새로이 개발된 비탁법을 이용한 혈청 저밀도 지단백 콜레스테롤의 직접 측정법의 평가

민도식,김덕언,박일규 한양대학교 의과대학 1999 한양의대 학술지 Vol.19 No.1

The association between elevated levels of low-density lipoprotein cholesterol (LDL-C) and increased risk of premature coronary heart disease has been clearly demonstrated. Currently, most of laboratories rely on indirect measurement of LDL-C by using Friedewald equation which are not applied in samples of non-fasting or over 400mg/dL of triglyceride level. For these reasons, a newly developed direct LDL-C assay by turbidimetric method was evaluated and compared with Friedewald equation. Also, we evaluated the quality control materials which are made in our laboratory. Total cholesterol, triglyceride, HDL-C, direct LDL-C and Friedewald LDL-C are measured on commercial control sera, two patient's pooled sera and patient sera using automated chemistry analyzer (Hitachi-747, Japan). Direct LDL-C assay using LDL Cholesterol homogeneous (Boeringer Mannheim, Germany) was performed using automated chemistry analyzer (Hitachi-747, Japan). Within-run precisions of Friedewald LDL-C and direct LDL-C were 113.83±3.70 mg/dL (3.31%) and 124.83±2.25 mg/dL (1.80%), respectively in control sera. Between-run precision were 112.53±2.60 (2.31%) and 120.64±2.39 (1.98%) repectively. The correlation coefficient between Friedewald LDL-C and direct LDL-C methods in 64 patient's samples was 0.87 and the linear regression equation was Y = 0.593X + 40.868 at triglyceride levels below 400 mg/dL. There was no significant decrease in LDL-C determined by direct LDL-C assay for non-fasting versus fasting sera and their values were 97.2±42.9 mg/dL, 95.9±41.0 mg/dL respectively. In two pooled sera, intra-assay CV of lyophilized serum which was restored by 1 mL distilled water was 1.84% and the other serum which was thawed after freezing was 17.90%. Precisions of direct LDL-C using LDL Cholesterol homgeneous were better than calculated LDL-C values using Friedewald equation in control sera. There was a good correlation between two methods with triglyceride levels below 400 mg/dL. Thus this direct LDL-C assay is recommended in place of Friedewald equation which has a few limitation for use. And the lyophiized sera from pooled sera is recommended to use in quality control instead of company-made control sera which are expensive and have many shortcoming.

균질법을 이용한 혈청 저밀도 지단백 콜레스테롤의 직접측정

이미경,이동수,박애자 대한임상검사정도관리학회 1999 臨床檢査와 精度管理 Vol.21 No.1

면역분리법에 의한 혈청 저밀도지단백콜레스테롤의 직접측정에 관한 연구

홍기숙 대한임상병리학회 1997 대한임상병리학회지 Vol.17 No.6

제조업 근로자의 공복 시 혈당과 혈청지질 및 비만지표와의 관련성

박승경(Sung-Kyeong Park),조영채(Young-Chae Cho) 한국산학기술학회 2017 한국산학기술학회논문지 Vol.18 No.4

본 연구는 제조업 근로자들의 공복 시 혈당과 혈청지질치(TC, TG, HDL-C, LDL-C) 및 비만지표(BMI, 체지방률, 허리둘레, 허리둘레와 엉덩이둘레의 비)와의 관련성을 검토할 목적으로 시도하였다. 연구는 2015년 1월부터 12월까지의 기간에 한 대학병원 건강검진센터에서 종합건강검진을 받았던 30~59세의 근로자 1,473명을 대상으로 하였다. 자료의 분석은 조사대상자의 공복 시 혈당을 정상군과 비정상군으로 구분하여 혈청지질치 및 비만지표의 평균치를 비교하였고, 성과 연령을 조정한 다중 회귀분석을 통해 공복시 혈당에 영향을 미치는 관련요인을 검토하였다. 연구결과, 조사대상자의 TC, TG, LDL-C, BMI 및 허리둘레는 공복 시 혈당이 정상인 군보다 비정상인 군에서 유의하게 높았고, HDL-C는 공복 시 혈당이 정상군보다 비정상군에서 유의하게 낮았다. 조사대상자의 공복시 혈당치는 TC, TG, LDL-C, BMI 및 허리둘레와 유의한 양의 상관관계를 보였다. 공복 시 혈당에 영향을 미치는 요인으로는 TC, TG, BMI 및 체지방률이 유의한 변수로 선정되었다. 이상과 같은 결과는 제조업 근로자들의 공복시 혈당은 TC, TG, LDL-C와 같은 혈청지질치 및 BMI, 체지방률과 같은 비만지표와 유의한 관련성이 있음을 시사한다. The purpose of this study was to investigate the relationship between the fasting blood sugar and serum lipid levels (TC, TG, HDL-C, LDL-C) and obesity indices (BMI, body fat rates, waist circumference, waist to hip ratio). The study sample consists of 1,473 manufacturing workers aged from 30 to 59 years, who underwent a health check-up at a university hospital during the period from Jan. to Dec. 2015. A data analysis was conducted to classify the subjects into the normal and abnormal groups according to their fasting blood sugar levels depending on the average values of the serum lipids and obesity indices. Multiple regression analyzes adjusted for sex and age were conducted for the factors affecting the fasting blood sugar level. As a result, the Serum TC, TG, LDL-C, BMI and waist circumference were found to be significantly higher in the abnormal fasting blood sugar level group than in the normal one, but the HDL-C was significantly lower in the abnormal group than in the normal one The fasting blood sugar level had a significant positive correlation with the TC, TG, LDL-C, BMI and waist circumference. The TC, TG, BMI and body fat were the significant factors affecting the fasting blood sugar. The above results suggest that the fasting blood sugar and serum lipid levels (TC, TG, HDL-C, LDL-C), obesity indices (BMI, body fat rates, waist circumference, waist to hip ratio) of manufacturing workers are significantly associated with each other.

신생아에서의 HDL-C와 LDL-C 値에 관한 연구

한병길,정용헌 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.1

The values of serum HDL-C and LDL-C were measured in 70 newborn infants with gestational ages from 35-43 weeks, birth weight from 2000 to 4500 gm, Apgar score 7 or more at 1 & 5 minutes and normal physical examination. 1. In all newborn infants, the mean HDL-C values & ranges were 31.99 ±6.67 mg/dl, from 21.5 to 49.8mg/dl, and the mean LDL-C values & ranges were 41.42 ±21.39mg/dl from 15.1 to 94.3mg/dl. 2. In term newborns with AGA, the mean HDL-C & LDL-C values were 32.85 ±6.75mg/dl and 45.85 ±21.39mg/dl respectively. 3. The HDL-C and LDI-C values of female were significantly higher than those of male. 4. The correlation of HDL-C by weight for gestational age was not significant, but that of LDL-C value was significantly lower in SGA than those of AGA & LGA. 5. The correlations of HDL-C & LDL-C according to birth weight, gestational age and Ponderal Indices were not significant.

웨이트트레이닝 시 DHEA섭취가 혈중 테스토스테론과 지질에 미치는 영향

이상호,박성진,한상철,김기홍,유기성,공창빈,송상협,홍지영 대한운동사협회 2010 아시아 운동학 학술지 Vol.12 No.2

[INTRODUCTION] This study attempted to examine the effect on testosterone, blood lipid according to the intake of DHEA during the 10 weeks of weight training for healthy 20 male college students. [METHOD] The experimental group took 100mg of DHEA and performed weight training but the control group did weight training only. [RESULT] The results of this study are as follows: the change in testosterone was greater in the experimental group than in the control group. Regarding blood lipid, the significant changes in total cholesterol were observed in both group but there were no statistic differences between two groups. There were no significant differences between two groups in HDL-C, as well. The significant changes in LDL-C were observed in both groups. However, there were no statistic differences between two groups. [CONCLUSION] the changes of testosterone and fat free mass were greater in the experimental group than in the control group whereas no significant changes in total cholesterol, HDL-C, LDL-C, were observed in both groups. Thus, the result shows that the intake of DHEA with weight training potentiates the positive effect on improvements of muscle, increasing testosterone and the change of fat free mass. [서론] 본 연구는 신체 건강한 남자 대학생 20명을 대상으로 10주간의 웨이트 트레이닝시 DHEA섭취가 테스토스테론, 지질 및 신체구성 변화에 미치는 영향을 알아보고자 실시하였다. [방법] 실험군은 웨이트 트레이닝에 참여하면서 DHEA 100mg을 섭취하고, 대조군은 웨이트 트레이닝만 참여하였다. [결과] 테스토스테론의 변화에서는 실험군이 대조군보다 통계적으로 유의하게 증가하였다. 지질의 변화에서는 TC의 경우 실험군과 대조군 모두 유의하게 감소하였으나, 집단 간 차이검정에서는 통계적으로 유의한 차이가 없었다. HDL-C의 경우 실험군과 대조군의 유의한 차이가 없었다. LDL-C의 경우 실험군과 대조군 모두 유의하게 감소하였으나, 집단 간 차이검정에서는 통계적으로 유의한 차이가 없었다. 신체구성의 변화에서는 제지방 변화량의 경우 실험군이 대조군보다 유의하게 증가하였고, 체중과 제지방량, 체지방률의 경우 실험군과 대조군의 유의한 차이를 보이지 않았다. [결론] 이상의 연구결과로 미루어 테스토스테론과 제지방 변화량은 실험군이 대조군에 비하여 유의한 증가를 나타냈으나, TC, HDL-C, LDL-C, 체중, 제지방량, 체지방률은 유의한 차이를 보이지 않았다. 따라서 웨이트 트레이닝시 DHEA의 섭취는 테스토스테론과 제지방량을 증가시켜 근육발달에 긍정적인 영향을 미치는 것으로 나타났다.

비타민 C 과량 섭취가 혈액에 미치는 영향

최우순 한국중독범죄학회 2017 한국중독범죄학회보 Vol.7 No.3

비타민 C의 과량 섭취가 인체에 어떤 영향을 나타내는지 생화학적검사 및 혈액학적 검사를 통해 알아보고자 하였다. 본 실험은 비타민 C 500mg을 섭취 전과 섭취 후를 비교분석하였다. 생화학적 검사결과 총콜레스테롤은 섭취 전 176.09±24.37 mg/d, 섭취 후 149±15.88 mg/d로 유의성을 보였으며 (p<0.001), LDL-콜레스테롤은 섭취 전 141.78±21.39 mg/dL, 섭취 후 114.10±12.73 mg/dL, 그리고 혈당은 섭취 전 83.58±16.43 mg/dL, 섭취 후 70.86±5.33 mg/dL로 모두감소하였다(p<0.001). 혈액학적 검사결과에서 백혈구수(WBC), 적혈구수(RBC) 혈색소(Hb), 적혈구용적률(HCT) 그리고 혈소판(PLT)이 모두 감소하였다(p<0.05). 결론적으로, 비타민 C의 과량 섭취는 총콜레스테롤과 LDL콜레스테롤의 수치가 낮아져 심장질환 개선에 도움을 주는 것으로 나타났다. 또한 당뇨병과 관련있는 혈당 수치도 감소하여개선효과가 있는 것으로 나타났다. 하지만 혈액학적검사에서 백혈구수, 적혈구수, 혈소판수가 감소하여 혈액질환에 영향을 줄 수 있는 것으로 나타났다. 비타민 C 섭취시 과량섭취하지않도록 주의해야 할 것으로 사료된다. The purpose of this study was to investigate the effect of vitamin C overdose on the human body through biochemical and hematological tests. In this experiment, 500 mg of vitamin C was compared before and after ingestion. Biochemical tests showed that total cholesterol was 176.09 ± 24.37 mg/dL before intake and 149 ± 15.88 mg/dL after ingestion (p<0.001), and LDL-cholesterol was 141.78 ± 21.39 mg/dL before ingestion 114.10 ± 12.73 mg/dL, and blood glucose was decreased to 83.58 ± 16.43 mg/dL before intake and 70.86 ± 5.33 mg/dL after ingestion, respectively (p<0.001). Hematologic tests showed a decrease in WBC, RBC hemoglobin, hematocrit, and platelet counts (p<0.05). In conclusion, over-intake of vitamin C has been shown to lower the levels of cholesterol and LDL-cholesterol and to help improve heart disease. In addition, blood glucose levels associated with diabetes were also reduced, indicating improvements. However, hematologic tests showed that leukocyte count, red blood cell count, and platelet count decreased (blood diseases could be affected).

한국 성인에서 혈중 LDL입자의 크기에 영향을 미치는 관련 인자 연구

서연경,최혜미,김형숙,김정숙,김철호 대한지역사회영양학회 2004 대한지역사회영양학회지 Vol.9 No.1

The purpose of this research was to examine the relationship between the plasma LDL particle size and blood lipid profile, dietary factors and anthropometric values (body mass index, waist circumference and waist/hip ratio). The subjects were 173 adults aged 23 to 81 years, selected from the Outpatient Clinic and Cardiovascular Department of the Seoul Municipal Hospital. Dietary data were obtained using a 3-day food record and analyzed using Korean and US nutrient databases. The subjects were divided into three groups by LDL particle size:type A (large buoyant LDL, > 25.5 nm, n = 96), type I (Intermediate LDL, 25.2 ≤ - ≤ 25.5 nm, n = 18), and type B (small dense LDL, < 25.2 nm, n = 59) groups. The type B group had higher age, waist circumference, and waist/hip ratio (WHR) than the type A and type I groups. Serum concentration of triglyceride, Apo B, LDL/HDL cholesterol ratio and atherogenic index were significantly higher in the type B group as compared to those in the other two groups. HDL cholesterol level and Apo A-I/Apo B ratio were significantly lower in the type B group than the other two groups. The plasma LDL particle size was highly correlated with triglyceride (r = -0.450), Apo B (r = -0.402) and HDL cholesterol (r = 0.418). However, there was no correlation between plasma LDL particle size and dietary intakes. This study showed that small dense LDL was an important biochemical risk factor that was associated with other risk factors. (Korean J Community Nutrition 9(1) : 58 ~ 65, 2004)