http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Helicobacter pylori와 대장균의 Shuttle Vector 개발
조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-
In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.
李相範,姜成德,徐泳圭 단국대학교 1992 論文集 Vol.26 No.-
Recently, many algorithms for the image pattern recognition using the neural network have been proposed. In this paper, the characteristic parameters of signature pattern can be extracted by using discrete consine transform. The neural network is implemented with back propagation, generalized delta rule which is able to learn multi-layer perceptron among the various model and it is applied to the signature verification system as pattern classifier. Through the experimentation, the input of signature pattern which has N=16, is transformed with 2-dimension discrete cosine transform, and the characteristic parameters is applied to 3-layer perceptron neural network model. The experimental result shows the signature verification could be possible to verify them and the applied algorithms of neural network model could be applied to the signature verification.
음향방출 신호를 이용한 SWS 490A 고장력강의 접합성 평가에 대한 실험적 연구
이장규,우창기,윤종희,조진호,조대희,박성완,김봉각,구영덕 한국공작기계학회 2004 한국공작기계학회 추계학술대회논문집 Vol.2004 No.-
The object of this study is to investigate the effect of joinability by using acoustic emission (AE) signals and doing a pattern recognition for weld heat affected zone (HAZ) in tensile testing. This study was carried out an SWS 490A high tension steel for electric shielded metal arc welding (SMAW), CO₂ gas arc welding and TIG welding. And correspondingly, the root openings are 3, 4 and 2.8㎜. The results of the tensile test of weld HAZ come out electric shield arc welding > CO₂ gas arc welding > TIG welding in case of single welding. It is believed that this is a phenomenon where difference of its root opening or base metal thickness. Also, the technique of AE is ideally suited to study variables which control time and stress dependent fracture or damage process in metallic materials.
내면화 이론과 초등학생의 자기조절 학습 : 외발적 성취동기화의 세 가지 양식을 중심으로
이영식,김대성 全北大學校 敎育大學院 1998 敎育論叢 Vol.18 No.-
This study aims at clarifying students' motivation. Educators are inclined to behold outer perspective, focusing on how to create conditions that facilitate learning, how to support. guide, inspire, and promote the learning process. But this issues can be attainable only through the inner perspective by looking at what it is inside the learner that leads them to focus on something, take interest, and assimilate it. But most school activities ate based on extrinsic motivation. which fully effective only by the process of internalization. This internalization categorized into three forms: external regulation introjected regulation, and identifiable regulation. Motivational development proceeds away from heteronomy in regulation and toward autonomy under appropriate conditions. These conditions include provision of autonomy, adequate structure information, context of warm positive involvement on the part of socializers. This research has shown how both parents' and teachers' support for children's autonomy can enhance their self-regulatory capacities. This means that children can grow toward self-regulation if the environment gives over responsibility in accord with their developmental capacity and makes non-arbitrary, potentially meaningful demands.
SWS 490A 강의 용접 열영향부 음향방출 특성에 대한 연구(1)
이장규,우창기,박성완,윤종희,조진호,김봉각,구영덕 한국공작기계학회 2004 한국생산제조학회지 Vol.13 No.4
The object of this study is to investigate the effect of compounded welding through the AE(Acoustic Emission) characteristics for weld HAZ(Heat Affected Zone) under the static tensile test. This study was carried out an SWS 490A, high tension steel for electric shield metal arc welding(SMAW), CO₂gas arc welding and TIG welding. Data displays are based on the measured parameters of the AE signals, along with environmental variables such as time and load. The accumulated AE event curve of HAZ definitely have the point of inflection subject to tensile test. The results of the tensile test of HAZ come out electric shield arc welding > CO₂ gas arc welding >TIG welding in case of single welding, but generally the tensile test of HAZ come out electric shield arc welding > TIG welding > CO₂ gas arc welding. These history plots give us useful and powerful information to analyze the results of material evaluation testing.