Protective Effects of Portulaca oleracea L. Extract against Matrix Metalloproteinase Production and Reactive Oxygen Species Generation Induced by Ultraviolet B Radiation in Human Keratinocytes

Jung Hwan Oh(오정환),Fatih Karadeniz(파티 카라데니즈),Jung Im Lee(이정임),So Young Park(박소영),Youngwan Seo(서영완),Chang-Suk Kong(공창숙) 한국생명과학회 2018 생명과학회지 Vol.28 No.8

쇠비름(Portulaca oleracea.L)은 쇠비름과에 속하는 한해살이풀로서 리놀렌산과 같은 불포화지방산, 페놀성 화합물, 플라보노이드, 비타민 C, 미네랄 함량이 높은 것으로 보고되어 있다. 본 연구에서는 쇠비름 추출물을 이용하여 UVB를 조사한 인간각질형성세포에서 광노화 억제능을 확인하였다. Matrix metalloproteinases는 세포의 기질을 분해하는 효소로 MMP-1는 collagenase, MMP-2와 MMP-9는 gelatinases로 피부 진피층을 구성하는 type Ⅰ collagen을 분해시키는데 영향을 미친다. UVB를 조사한 인간각질형성세포에서 쇠비름 추출물을 처리했을 때 MMP-1, -2, -9의 발현이 감소하였으며, type Ⅰ procollagen의 발현은 증가하는 것으로 나타났다. 또한 쇠비름 추출물을 처리한 군에서 UV에 의한 ROS 생성이 감소하였는데 이는 Nrf-2의 활성화를 통한 항산화 인자 SOD-1과 OH-1의 발현 증가로 인해 세포내 ROS 생성이 감소한 것으로 사료된다. 따라서 본 연구 결과를 통해 쇠비름 추출물이 UVB를 조사한 인간각질형성세포에서 MMP 인자 및 항산화 인자의 발현 조절을 통해 광노화로부터의 세포 보호능을 가지는 것을 확인하였으며 나아가 화장품 소재로서의 개발 가능성을 확인하였다. Portulaca oleracea L. is an edible plant widely consumed in daily diet throughout Europe, Asia and America. In this study, protective effects of P. oleracea L. extracts against oxidative stress and matrix metalloproteinase (MMP) activity induced by ultraviolet B (UVB) radiation were investigated using HaCaT immortal human keratinocytes. In this context, the mRNA and protein productions of MMPs (MMP-1, -2, and -9) and type I procollagen, which are major markers of photoaging induced by UVB radiation in HaCaT keratinocytes, were evaluated. Furthermore, UVB-induced reactive oxygen species (ROS) generation and mRNA and protein expression levels of superoxide dismutase-1 (SOD-1), oxygenase-1 (OH-1), and nuclear factor-erythroid 2-related factor-2 (Nrf-2), all of which are associated with the antioxidant balance, were investigated. As shown by the results, UVB radiation induced ROS formation and led to increased production of MMPs and decreased collagen production in human keratinocytes, which resulted in skin photoaging or photodamage. The treatment with P. oleracea L. extracts downregulated MMP (MMP-1, -2, and -9) production and upregulated type I procollagen expression in UVB-induced HaCaT cells. Furthermore, treatment with the extracts decreased UVB-induced ROS generation and increased the expression of antioxidant enzymes, such as SOD-1 and OH-1, through the Nrf-2 pathway. Taken together, these results suggest that P. oleracea L. extracts could be a potential cosmeceutical agent for the prevention of skin photoaging or photodamage.

원위 대퇴골의 고립성 골연골종에서 발생한 속발성 연골 육종 1례 보고

정필현,황장수,강석,김용민,오형호,채동주,김종필 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

연골육종은 비교적 서서히 자라며 늦게 전이하는 악성 연골 종양으로 알려져 있다. 골 연골종에서 발생한 속발성 연골육종은 발생빈도는 드물며 타부위로의 전이가 적고 악성도가 낮은 경우가 많아 적합한 수술적 치료로서 좋은 예후를 보이는 것으로 보고되고 있다. 본 동국대학교 정형외과학 교실에서는 우측 대퇴골 원위부 골간단부에 생긴 고립성 골 연골종에서 이차적으로 발생한 연골육종을 치험한 바 문헌고찰과 함께 보고하는 바이다. A case of Secondary Chondrosarcoma Arising from Solitary Osteochondroma of the Distal Femur Department of Orthopaedic Surgery, College of Medicine, DongGuk University Phil Hyun Chung M.D., Jung Su Hwang M.D., Suk Kang M.D., Yang Mln Kim M.D., Hyung Ho Oh M.D., Dong Ju Chae M.D., Jong Pil Kim M.D. Chondrpsarcoma is a malignant cartilage-forming tumor that grows slowly and metastasis of this tumor occurs in late stage. Secondary chondrosarcomas arising from a solitary osteocartilaginous exostosis are rare and those have a better prognosis than other chondrosarcomas, and they rarely metastasize. We present our experience with a case of secondary chondrpsarcoma arising in solitary osteochondroma of distal metaphysis of right femur with clinical details.

수행에 대한 지식의 상대적 빈도가 편마비 환자의 균형 재훈련에 미치는 영향

오동식,최흥식,김택훈,노정석 한국전문물리치료학회 2001 한국전문물리치료학회지 Vol.8 No.1

During therapy sessions, feedback is often provided concurrently by the physical therapist as the patient attempts to perform a movement and after the movement attempt. This feedback is provided to enhance the patient's balance abilities. However, recent studies in nondisabled populations have suggested that frequent feedback may be detrimental to retention or learning of motor skills. This study compared the effects of 100% relative frequency of knowledge of performance (KP) with 66% relative frequency of KP for motor learning on balance retraining in patients with hemiplegia. Twenty patients with hemiplegic were randomly assigned to one of two experimental groups. The acquisition phase consisted of 16 blocks of 5 trials for 2 days (80 total practice trials). The retention chase consisted of 2 blocks of a short-term retention test, one day after the end of the acquisition phase and a long-term retention test, one week after the end of the short-term retention test. In the 100% feedback condition, participants received feedback after every practice trial. A faded KP schedule was used in the 66% condition. No significant differences were found between the two groups during all experimental phases (acquisition and retention phases), (p>.05). However, there were significant decreases in balance index for both groups of acquisition phase (p<.05). These results suggest that 66% relative frequency of KP is not more effective than 100% relative frequency of KP with respect to retention over time when hemiparetic patients attempt to learn balance.

체육에 있어서 주관적 체험의 가치

오정석 인천교육대학교 초등교육연구소 2001 교육논총 Vol.18 No.-

인간의 몸에 대한 우리의 태도는 이중적이다. 대부분 인간의 몸을 단순한 영혼의 거주처로서 뿐만 아니라 참된 인식의 방해물로 생각하는 경향이 있다. 더구나 과학주의의 틈바구니에서 인간의 몸은 마치 연료가 있는 한 계속 달릴 수 있는 자동차와 같이 취급되었다. 이런 관점으로는 자기의 몸 움직임을 체험할 수 없다. 이때 체육활동은 생명감이 없다. 연습은 격렬할 순 있으나 지루하다. 이 문제의 해결은 주관적인 몸과 그 체험에 대한 바른 해석으로부터 시작된다. 체육활동은 인간의 몸 움직임을 주제로 지향적, 의도적, 근운동감각적 노력을 통해 갖추어가는 주관적-능동적 활동의 방식이다. 운동은 우리로 하여금 온 몸으로 탈 대상화를 체현하는 계기를 제공한다. 많은 체육교육자들은 우리 몸의 주관적 체험을 지지한다. 교육적으로 추구하는 목적은 주체적인 몸 활동의 체험으로만 가능하며, 개인적으로도 몸 움직임은 값진 의미를 지니게 되기 때문이다. 이러한 몸 움직임의 주관적 체험은 자유로운 자아의 실현이다. 체육활동은 이러한 기회를 마련해주는 교육의 장이다. The purpose of this paper is to examine values of subjective experience in physical education so that its objectives and nature may be ascertained and an appropriate course of development might be found. Physical activity is not an act of observation but requires direct participation. The thing which enhances this experience is the imagination. The imagination helps us take the experience of human movement to a higher level. The point which physical education must convey is that which the individual personally discovers through direct experience. Therefore the essence of physical education can be summed up as "realization through movement".

空氣中 正極性 Spark Channel의 磁氣場에 의한 影響

吳喆漢,尹文洙,姜禎錫 慶北大學校 産業開發硏究所 1978 硏究報告 Vol.6 No.-

The influences of magnetic field on the positive spark channel of point-to-rod gap under steady potentials in air were studied by means of two cameras and an oscilloscope. The direction of magnetic field was perpendicular to the gap axis, and the magnetic field strength ranging from 0 to 6.6kG was applied. The positive spark channel was deflected in the direction of the Lorentz force. Therefore, it was confirmed that the positive streamer model, which says the streamer is progressing with positive space charge on its tip, is reasonable. The first step length of the spark channel was increased as the magnetic field strength was increased, but it was abruptly decreased at about 4kG. Then, it showed constant above 5kG. However, it first step deflection length was increased at about 3kG. But it was decreased from 3kG to 4kG. It was unchanged above 5kG.

벼 담수직파 재배시 생장조절제 처리가 생육 및 도복에 미치는 영향

金正鎬,吳成煥,李哲遠,宋凡憲,孫錫龍 忠北大學校 農業科學硏究所 2002 農業科學硏究 Vol.19 No.-

본 시험은 벼 도복을 경감시키기 위한 재배대책을 강구하기 위하여 추청벼를 공시하고, 담수직파재배에서 생장조절제를 처리함에 따른 도복경감효과를 조사하였던 바 그 결과를 요약하면 다음과 같다. 1 간장은 무처리구보다 IBP+Inabenfide구가 186cm 단축되어 가장 큰 감소효과를 보였다 2 하위절간장 역시 무처리구보다 IBP+Inabenfide구가 3-4절간 35cm, 4-5절간은29cm 단축되어 가장 큰 감소효과를 보였다 3 포장도복은 무처리 5에 비해 IBP+Inabenfide구는 0으로 도복이 발생하지 않았으며, 수량은 무처리에 비해 도복이 발생하지 않았던 IBP+Inabenfide구가 5% 증수되었다 This study was conducted to investigate the effects of plant growth regulators, 2,4~D, IBP, and Inabenfide, on growth responses and lodging resistance of rice in cultivation of the waterlogging direct seeding The Chuocheong cultivar of Japonica type was used Growth responses itcluding culm leagth and lodging index etc and rice yields were measured at major growth stages and harvest time Culm length was the shortest in the treatment of IBP + Inabenfide, shortening it to 186 cm compared to that of the control, showing the best application effect of the plant growth regulator The length of internode between third and fourth internodes of lower culm was shortened to 3.5 cm with treatment of IBP + Inabenfide compared to that of the control and it between fourth and fifth internodes was shortened to 29 cm with treatment of Inabenfide, showing the best effectiveness. Lodging was not appeared in treatment of IBP + Inabenfide compared to 5.0 of the control Resulting to reduciag the lodging, rice yield was increased more with treatment of IBP + Inabenfide, to about 50%, than that of the control

벼 재배양식에 따른 규산질 비료 추비 사용 효과

金正鎬,吳成煥,李哲遠,宋凡憲,孫錫龍 한국국제농업개발학회 2002 韓國國際農業開發學會誌 Vol.14 No.1

본 시험은 벼 도복을 경감시키기 위한 재배대책을 강구하기 위하여 광안벼를 공시하고, 이앙재배와 담수직파재배에서 규산질 비료를 사용함에 따른 도복경감효과를 조사하였던 바 그 결과를 요약하면 다음과 같다. 1. 간장은 담수표면직파재배에서 규산시용구가 무처리보다 3.1cm 감소하여 3.7%가 단축되었으며, 기계이앙재배에서는 규산시용구가 무처리보다 5cm 감소하여 5.5% 단축되었다. 2. 하위절간장은 담수표면직파재배에서 규산시용구가 무처리보다 3.1cm 감소하여 36.9% 단축되었으며, 기계이앙재배에서는 규산시용구가 무처리보다 1.2cm 감소하여 11% 단축되었다. 3. 좌절중은 담수표면직파에서 규산시용구가 73g이 증가하여 35.2%의 증가율을 보였고, 기계이앙재배에서는 규산시용구가 37.3g 증가하여 9.6%의 증가율을 보였다. 4. 수량은 담수표면직파재배에서 규산시용구가 무처리보다 30kg이 많아 6.4%의 증수효과를 나타내었고, 기계이앙재배에서는 규산시용구가 무처리보다50kg이 많아 9%의 증수효과를 나타내었다. This study was carried out to examine the responses of growth and lodging of rice as affected by both two cropping practices, transplanting and direct seeding in waterlogging, and different application amounts of silicate fertilizers. The Kwangan cultivar of Japonica type was used, the growths including culm and internode lengths and grain yield were investigated in major growth stages and harvest time. The culm length in waterlogging direct seeding was reduced in treatments of silicate fertilizers, about 3.7%, compared to that in the control and it was reduced to 5.5% in the transplanting cultivation. The breaking strength of lower internodes of rice were increased to 35.2% and 9.6% by applying of silicate fertilizers in the water direct seeding and in the transplanting cultivations, respectively. The rice yields were increased with application of silicate fertilizers in both water direct seeding and transplantingcultivations compared to those of the control, about 6.4% and 9.0%, respectively.

벼 재배방법과 규산질 비료 시용이 생육 및 도복에 미치는 영향

金正鎬,吳成煥,李哲遠,宋凡憲,孫錫龍 忠北大學校 農業科學硏究所 2002 農業科學硏究 Vol.19 No.-

본 시험은 벼 도목을 경감시키기 위한 재배대책을 강구하기 위하여 광안벼를 공시하고, 이앙재배와 담수직파재배에서 규산질 비료를 시용함에 따른 도복경감효과를 조사하였던 바 그 결과를 요약하면 다음과 같다 1 간장은 담순표면직파재배에선 규산시용구가 무처리보다 31cm 감소하여 37%가 단축되었으며, 기계이앙재배에서는 규산시용구가 무처리보다 5cm 감소하여 55% 단축되었다 2 하위절간장은 담수표면직파재배에서 규산시용구가 무처리보다 3.1cm 감소하여 36 9% 단축되었으며, 기계이앙재배에서는 규산시용구가 무처리보다12cm 감소하여 11% 단축되었다 3 좌절중은 담수표면직파에서 규산시용구가 73g이 증가하여 35 2%의 증가율을 보였고, 기계이앙재배에서는 규산시용구가 373g 증가하여 9.6%의 증가율을 보였다 4 수량은 담수표면직파재배에서 규산시용구가 무처리보다 30kg이 많아 64%의 증수효과를 나타내었고, 기계이앙재배에서는 규산시용구가 무처리보다50kg이 많아 9%의 증수효과를 나타내었다 This study was carried out to examine the responses of growth and lodging of rice as affected by both transplanting and direct seeding in waterlogging, which was differently applicated amounts of silicate fertlizers The Kwangan cultivar of Japonica type was used The growths including culm and internode lengths and grain yield were investigated in major growth stages and harvest time The culm length in waterlogging direct seeding was reduced in treatments of silicate fertilizers, about 37%, compared to that in the contro1 and it was reduced to 5.5% in the transplanting cultivation The breaking strength of lower internodes of rice were increased to 35.2% and 96% by applying of silicate fertilizers in the water direct seeding and in the transplanting cultivations, respectively The rice yields were increased with application of silicate fertilizers in both water direct seeding and transplanting cultivations compared to those of the control, about 64% and 90%, respectively.

정상 관동맥을 가진 심장 점액종 환자에서 발생한 급성 심근 경색증 1예

이정민,이현승,전은정,윤호중,정욱성,오용석,김철민,김재형,최규보,홍순조,이교영,심상인 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.9

최근 9년간 신경정신과에 입원한 환자에 대한 임상적 고찰(1972∼1980년)

이정호,안일남,오석환,정영조,최영민,원장섭,김영훈,하혜경,이승탁,이영주 인제대학교 1983 仁濟醫學 Vol.4 No.2

정신질환 환자들에 대한 정확한 생태를 파악함은 정신 건강과 치료 대책을 세우는데 중요하다. 이에 본 저자들은 1972년부터 1980떤 사이에 인제의대 부속 서울백병원 신경정신과에 입원하여 치료 받았던 환자들에 대한 제반 요인들을 분석 검토하였다. Clinical charactristics of neuropsychiatric inpatients were evaluated and discussed with special referrence to their diagnostic distribution. All discharged cases from neuropsychatric ward of Seoul Paik Hospital during nine years from 1972 to 1980 were 2863. Among them 813 cases were discarded from this study because their clinical charts were vague in description of failed to find out. The materials of this study were extracted from 2050 discharged cases. Total number of cases of inpatients were gradually increasing by each year from 1972 to 1980. Psychoneurosis was diagnosed in 45.2%, schizophrenia in 26.9%, organic brain syndrome in 8.9%, major affective disorder in 5.5%, personality disorder in 4.8%, other psychiatric disorder in 1.5%, psychophysiologic disorder in 1.0%, mental retardation in 0.5%, paranoid state in 0.5%, and other psychosis in 0.3%. The gruop of patients, ranging in age from 21 to 40 was the highest (75.2%) in this study. Male patients were 43.9% and female patients were 56.1%. Schizophrenia leas diagnosed in 32.9%, depressive neurosis in II.3%, anxiety neurosis in 9.3%, and personality disorder in 8.1% in male patients. Hysterical neurosis was diagnosed in 25.5%, depressive neurosis in 22.2%, schizophrenia in 22.2%, and anxiety neurosis in 7.0% in female patients. The authors evaluated the route of admission of patients. Sixty-eight point three percent of total studied cases were admitted through outpatient department, 20.9% of cases were admitted through emergeney room, and 8.2% of cases were transfered from other department or hospital. Among patients who were admitted to psychiatric wards through emergency room and transferred from other department or hospital, psychoneurosis was the hightest diagnostic group in rate. Most of patients who were transferred from other department or hospital were from Internal Medical Department. There were no relationship between seasonality of admission and diagnostic group in this study. readmission rate of total studied patients in neuropsychiatric department of Seoul Paik Hospital was 30%. The readmission rate of personality disorder, mental retardation, schizophrenia, and major affective disorder were relatively high. The cases who were discharged against medical advice were 441. Among them misunderstanding about psychiatric treatment was predominent. Twenty seven percent of patients had physical illness at or during admission. The patients who were diagnosd as psychophysiological disorder had physical illness in 42.8%, 35.4% in personality disorder, 33.9% in major affective disorder, and 24.3% in schizophrenia. About nine percent of total studied cases had the history of attempted suicide. Major affective disorder, Personality disorder, Deressive neurosis, and Other Psychosis showed high incidence of history of attempted suicide prior to admission.