가압가열 및 microwave에 의한 중력분 반죽 gliadin의 항원성 변화

곽지희(Ji-Hee Kwak),김꽃봉우리(Koth-Bong-Woo-Ri Kim),이청조(Chung-Jo Lee),김민지(Min-Ji Kim),김동현(Dong-Hyun Kim),선우찬(Chan Sunwoo),정슬아(Seul-A Jung),김현지(Hyun-Jee Kim),최정수(Jung-Su Choi),김성원(Seong-Won Kim),안동현(Dong-Hyun 한국식품과학회 2012 한국식품과학회지 Vol.44 No.1

본 연구에서는 가압가열 및 microwave 처리에 의한 중력분 반죽 추출물 내의 gliadin 단백질의 항원성 변화에 대해 살펴보았다. 중력분 반죽에 가압가열과 microwave를 단독 또는 병행으로 처리하여 ci-ELISA, SDS-PAGE 및 immunoblotting을 실시하였으며, 가압가열 처리에 의해서 anti-gliadin IgG 항체와 gliadin과의 결합력이 다소 감소한 것을 확인하였다. 특히 30 min 이상 처리시 더욱 감소한 것으로 나타났으며, SDS-PAGE와 immunoblotting 결과에서도 gliadin band의 강도가 약해지고 항체와의 반응도 나타나지 않았다. Microwave 처리의 경우, 5 min 이상 처리시 일부 gliadin 단백질의 소실이 관찰되었으나, 항원성에는 큰 변화가 없었다. 또한 가압가열 및 microwave 병행 처리에 의해 항원-항체 결합력이 더욱 감소되었으며, 특히 가압가열 50 min, microwave 10 min 처리시 약 35.0%로 감소되었다. 이상의 결과를 통해 가압가열 처리에 의해 중력분 반죽 추출물 내 gliadin의 항원성이 감소되는 것을 확인하였으며, microwave와 병행 처리하는 경우, 더욱 감소하는 것을 확인하였다. The aim of this study was to determine the optimal physical treatment to reduce the antigenicity of gliadin in wheat dough. Medium wheat dough was treated with an autoclave (5, 10, 30, and 50 min at 121℃, 1 atm), a microwave (1, 5, and 10 min) or both (10, 30, and 50 min/5, 10 min). The proteins in the dough extracts were analyzed by SDS-PAGE and the binding ability of anti-gliadin IgG to gliadin was examined by ci-ELISA and immunoblotting. Results showed that the ability of anti-gliadin IgG to bind to gliadin in wheat dough treated with an autoclave alone or in combination with a microwave was decreased. Especially, it declined to ~77% after autoclaving for 30 min and 35% after both autoclaving for 50 min and microwaving for 5 min. In addition, the intensity of gliadin bands in SDS-PAGE were weakened and anti-gliadin IgG did not recognize gliadin in immunoblotting. However, microwaving alone did not affect the antigenicity of gliadin in wheat dough. These results indicate that autoclaving may affect the reduction of the antigenicity of gliadin in medium wheat dough. Moreover, autoclaving in combination with microwaving is more effective for reducing the antigenicity of wheat dough.

가압가열 및 Microwave 처리에 의한 중력분 Gliadin의 항원성 변화

곽지희(Ji-Hee Kwak),김꽃봉우리(Koth-Bong-Woo-Ri Kim),이청조(Chung-Jo Lee),김민지(Min-Ji Kim),김동현(Dong-Hyun Kim),선우찬(Chan Sunwoo),정슬아(Seul-A Jung),강주연(Ju-Youn Kang),김현지(Hyun-Jee Kim),최정수(Jung-Su Choi),김성원(Seong-Won 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.10

본 연구에서는 가압가열 및 microwave 처리가 gliadin의 항원성에 미치는 영향을 살펴보기 위해 중력분에 가압가열과 microwave를 단독 또는 병행으로 처리하여 Ci-ELISA, SDS-PAGE 및 immunoblotting을 실시하였다. 가압가열 처리의 경우, 처리 시간이 길어질수록 IgG와의 결합력이 감소하였으며, 특히 50분 처리구에서 약 69%로 가장 낮은 결합력을 보였다. 또한 SDS-PAGE와 immunoblotting 결과에서도 무처리구에서 강하게 보였던 gliadin band가 처리에 의해 거의 소실되고 항체와 반응하지 않았다. 가압가열 및 microwave를 병행 처리한 경우도 마찬가지로 gliadin의 결합력이 다소 감소하였으며, 처리구 중에서는 가압가열 50분, microwave 5분 처리구에서 약 73%로 가장 낮은 결합력을 보였다. 반면 microwave를 단독으로 처리하였을 때에는 일부 단백질의 변화는 관찰되었으나 항원성 감소에는 큰 영향을 미치지 않았다. 이상의 결과를 통해 가압가열을 단독 처리에 의해 gliadin의 항원성이 다소 감소되었으며, microwave 병행 처리에 의한 차이는 크게 나타나지 않은 것을 확인하였다. This study was conducted to evaluate the effect of physical treatments on the antigenicity of gliadin in medium wheat flour. The wheat flour was treated with an autoclave (5, 10, 30, 50 min), a microwave (1, 5, 10 min), and both (10, 30, 50 min/ 5, 10 min), and investigated by SDS-PAGE, immunoblotting and Ci-ELISA using anti-gliadin IgG. The results showed that the binding ability of anti-gliadin IgG to gliadin in wheat flour was slightly decreased when autoclaved or when autoclaved and microwaved. Especially, it was reduced to about 69% after autoclaving for 50 min and 73% after autoclaving for 50 min and microwaving for 5 min. In addition, gliadin bands in the 50 min autoclaved group disappeared in both SDS-PAGE and immunoblotting. On the other hand, the antigenicity of gliadin was unaffected by microwaving alone. Consequently, there were no considerable changes in using an autoclave alone or in combination with a microwave. These results suggest that autoclaving may affect the reduction of the antigenicity of gliadin in medium wheat flour.

Factors Influencing the Acrylamide Content of Fried Potato Products

Jin, Yong-Ik,Park, Kyeong-Hun,Chang, Dong-Chil,Cho, Ji-Hong,Cho, Kwang-Su,Im, Ju-Sung,Hong, Su-Young,Kim, Su-Jeong,Nam, Jung-Hwan,Sohn, Hwang-Bae,Yu, Hong-Seob,Chung, Ill-Min The Korean Society of Environmental Agriculture 2016 한국환경농학회지 Vol.35 No.4

BACKGROUND: Acrylamide (CAS No. 79-06-1) is known to be a carcinogenic compound, and is classified as a Group 2A compound by the International Agency for Research on Cancer (IARC, 1994). Acrylamide can be generated during the browning process via the non-enzymatic Maillard reaction of carbohydrates such as reducing sugars and of amino acids such as asparagine, both of which occur at a temperature above $120^{\circ}C$. Potato tubers contain reducing sugars, and thus, this will affect the safety of processed potato products such as potato chips and French fries. In order to reduce the level of acrylamide in potato processed products, it is therefore necessary to understand factors that affect the reducing sugar content of potatoes, such as environmental factors and potato storage conditions, as well as understanding factors affecting acrylamide formation during potato processing itself. METHODS AND RESULTS: Potatoes were cultivated in eight regions of Korea; For each of these different environments, soil physico-chemical characteristics such as pH, electrical conductivity, total nitrogen, available phosphate, and exchangeable cation content were measured and correlations with potato reducing sugar content and potato chip acrylamide levels were examined. The reducing sugar content in potato during storage for three months was determined and acrylamide level in potato chip was analyzed after processing. The storage temperature levels were $4^{\circ}C$, $8^{\circ}C$, or $10^{\circ}C$, respectively. The acrylamide content of chips prepared from potatoes stored at $10^{\circ}C$ or $20^{\circ}C$ for one month was analyzed and the different frying times were 2, 3, 5, and 7 min. CONCLUSION: This study showed that monitoring and controlling the phosphate content within a potato field should be sufficient to avoid producing brown or black potato chips. For potatoes stored at low temperatures, a reconditioning period ($20^{\circ}C$ for 20 days) is required in order to reduce the levels of reducing sugars in the potato and subsequently reduce the acrylamide and improve chip coloration and appearance.

수확후 채소류에 발생하는 진균독소의 탐색과 방제(Ⅱ) 이병된 양념 채소류(양파, 마늘, 고추)에서 주요 진균독소 검출

정일민,주호종,심성철,백수봉,유승헌 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

수확후 Alternaria, Penicillium 및 Fusarium에 이병된 고추, 양파, 마늘을 HPLC로 주요독소들을 검정한 결과 고추에서 Alternaria 독소의 AOH(alternariol)는 소량∼440㎍/g 및 ALT(altenuene)는 소량∼103㎍/g, TeA(tenuagonic acid)는 249∼342㎍/g 및 AME(alternariol monomethyl ether)는 206∼294㎍/g이 검출되었고 양파, 마늘에서 Penicillium 독소의 citrinin이 2.8∼18.4㎍/g, penicillin-G는 0∼439.0㎍/g, penicillic acid는 0∼10.2㎍/g 및 patulin은 0∼7.0㎍/g 검출되었다. 그리고 양파, 마늘에서 Fusarium 독소로는 fusaric acid가 0∼553.6㎍/g 검출되었을 뿐 deoxynivalenol과 nivalenol은 검출되지 않았다. The major mycotoxins were detected from peppers, onions and garlics infected postharvest pathogens, Alternaria, Penicillium and Fusarium. Analyses of the major mycotoxins were conducted using HPLC. Detected Alternaria mycotoxins per gram of infected postharvest peppers were alternariol (AOH) with amount ranged from small quantity to 440 ㎍/g, altenuene (ALT) with amount ranged from small quantity to 103 ㎍/g, tenuagonic acid (TeA) with amount ranged from 249 to 342 ㎍/g and alternariol monomethyl ether (AME) with amount ranged from 206 to 294 ㎍/g. Penicillium toxins per gram of infected postharvest onions and garlics were citrinin with amount ranged from 2.8 to 18.4 ㎍/g, penicillun-G with amount ranged from no detection to 439.0 ㎍/g, penicillic acid with amount ranged from no detection to small quantity and patulin with amount ranged from no detection to small quantity. Fusarium toxins per gram of infected postharvest onions and garlics were fusaric acid with amount ranged from no detection to 553.6 ㎍/g. However, deoxyrivalenol and nivalenol were not detected from onins and garlics infected by Fusarium.

T1계 고온초전도체용 {113}<121>집합조직 Ag 기판

김수영,정대영,시게오호리우찌,정우창,조경목,박익민 대한금속ᆞ재료학회 2006 대한금속·재료학회지 Vol.44 No.3

중공유리구에 고정화된 광촉매를 이용한 남조류의 증식억제

강민수,정익상,우승희,김성우,이동근 경상대학교 환경보전연구소 2001 環境保全硏究所報 Vol.9 No.-

Photocatalytic inactivation of Microcystisaeruginosa was carried out with the TiO₂-coated pyrex hollow glass beads under the illumination of UV light(370㎚ wavelength). After being irradiated with UV light in the presence of the TiO₂-coated pyrex glass beads, Microcystis aeruginosa, known as typical cyanobacteria, lost their photosynthetic activity, and the colonies of Microcystis cells were completely separated into individual spherical one. Complete photocatalytic inactivation of Microcystis aeruginosa was obtained in about 30min. Photosynthesis activity of the cyanobacteria could be inhibited effectively after being reacted photocatalytically with TiO₂ immobilized hollow glass ball.

중공유리구에 고정화된 TiO_2 광촉매를 이용한 수중 조류(藻類)의 광합성억제

강민수,정익상,조인철,김성우,이동근 경상대학교 환경보전연구소 2000 環境保全硏究所報 Vol.8 No.-

TiO_2 was coated on the surface of the hollow pyrex glass beads through a sol-gel method. The TiO_2-coated hollow glass bead was employed as a photocatalyst to inactivate the algae under the illumination of UV light. TiO_2 could successfully be immobilized as a film on the surface of the glass bead. The TiO_2 film had anatase form and the average thickness of the film was estimated to be 0.3㎛. Two species of algae. Anabaena, and Microcystis were taken for the inactivation experiments. They are commonly found in algal blooms in eutrophic waters. When irradiated by UV light in the presence of the TiO_2-coated glass beads. Anabaena cells and Microcystis cells were lost their photosynthetic activity. Complete photocatalytic inactivation of Anabaena and Microcystis was obtained in about 30min.

과민성 대장 증후군 환자에서 Colpermin 투여의 임상 효과

금민수,조창민,김대현,이창형,권영오,김성국,최용환,정준모 慶北大學校 醫科大學 1997 慶北醫大誌 Vol.38 No.1

목적 : 과민성 대장 증후군은 장기능 이상의 증상군으로, 인지할 수 있는 기질적 위장관 병태 생리가 없는 경우로 기능적인 장관의 이상 경축과 가장 밀접한 관련이 있을 것으로 생각된다. 그러나 치료면에서는 아직 뚜렷한 방침이 정립되어 있지 않고 다양한 방법들이 제시되고 있는 실정이다. 저자들은 장용성 캅셀로 조제된 박하유 성분인 Colpermin® 의 임상 효과를 평가하고자 본 연구를 시행하였다. 대상 및 방법 : 1996년 2월부터 1996년 11월까지 경북대학교병원 소화기 내과를 방문하여 과민성대장 증후군으로 진단된 34명의 환자를 대상으로 Colpermin® 을 1회 2캅셀씩, 1일 3회, 경구로 2주간 투여하였다. 결과 : 투여전의 증상의 빈도는 복통 88.2%(30/34) 및 설사 73.5%(25/34)를 호소하는 경우가 가장 많았으며, 그 다음으로 변비 50.0%(17/34), 복부 팽만 47.1%(16/34), 고창 38.2%(13/34)순이었다. 약물 투여후 증상별 개선율은 설사를 보였던 환자들에서 호전 11예와 매우 호전 11예로 88.0%(22/25)의 가장 높은 개선율을 보였고, 그 다음으로 복통 73.3%(22/30), 고창 69.2%(9/13), 변비 64.7%(11/17), 복부 팽만 56.3%(9/16)의 순이었다. 종합적인 증상의 개선도는 현저한 개선이 2예(5.9%), 중등도 개선이 9예(26.5%), 약간 개선이 18예(52.9%), 불변이 4예(11.8%), 악화가 1예(2.9%)였으며, 종합적인 유효율은 85.3%(29/34)였다. 약물의 부작용으로 3예의 환자에서 각각 복부 팽만의 발생, 변비의 악화, 복통의 발생이 있었으나, 그 외의 환자들에서는 아무런 부작용도 관찰되지 않았다. 결론 : 과민성 대장 증후군의 약물치료에 있어서 Colpermin®이 유용한 약제가 될 수 있을 것으로 기대된다. Irritable bowel syndrome (IBS) is a disease characterized by a collection of symptoms of intestinal dysfunctions, without any recognized organic gastrointestinal pathology. This study was performed to evaluate clinical effectiveness of peppermint oil (Colpermin®) in the treatment of patients with IBS. 34 patients (18 males and 16 females) with IBS were treated with peppermint oil 0.4ml (as menthol 176㎎) three times daily for 2 weeks and the efficacy of the treatment was evaluated according to the degree of the improvement. The improvement of symptoms was 88.0% (22/25) in diarrhea, 73.3% (22/30) in abdominal pain, 69.2% (9/13) in flatulence, 64.7% (11/17) in constipation, and 56.3% (9/16) in abdominal distension, respectively. Overall effectiveness of subjective symptoms was 85.3% (29/34). Side effects of the drug were noted in only 3 cases, those were aggravation of constipation, development of abdominal distension and abdominal pain. As a result, Colpermin® might be considered as an effective drug in relieving symptoms of IBS.

영구치 치수 기질세포를 이용한 연골 분화 및 분화 시기에 따른 형태학적 변화

정주령,김하나,박열,김민정,오영주,신수정,최윤정,김경호 大韓齒科保存學會 2012 Restorative Dentistry & Endodontics Vol.37 No.1

Objectives: The aim was to confirm the stem cell-like properties of the dental pulp stromal cells and to evaluate the morphologic changes during in vitro chondrogenesis. Materials and Methods: Stromal cells were outgrown from the dental pulp tissue of the premolars. Surface markers were investigated and cell proliferation rate was compared to other mesenchymal stem cells. Multipotency of the pulp cells was confirmed by inducing osteogenesis, adipogenesis and chondrogenesis. The morphologic changes in the chondrogenic pellet during the 21 day of induction were evaluated under light microscope and transmission electron microscope. TUNEL assay was used to evaluate apoptosis within the chondrogenic pellets. Results: Pulp cells were CD90, 105 positive and CD31, 34 negative. They showed similar proliferation rate to other stem cells. Pulp cells differentiated to osteogenic, adipogenic and chondrogenic tissues. During chondrogenesis, 3-dimensional pellet was created with multi-layers, hypertrophic chondrocyte-like cells and cartilage-like extracellular matrix. However, cell morphology became irregular and apoptotic cells were increased after 7 day of chondrogenic induction. Conclusions: Pulp cells indicated mesenchymal stem cell-like characteristics. During the in vitro chondrogenesis, cellular activity was superior during the earlier phase (within 7 day) of differentiation.

전류제어형 PWM컨버터를 이용한 동기발전기용 여자시스템에 관한 연구

장수진,류동균,서민성,김준호,원충연,이진국 성균관대학교 2003 학술회의지원논문목록집 Vol.2003 No.-

동기발전기 출력전압은 여자시스템의 계자전류 제어에 의해 일정하게 유지된다. 고주파 PWM 컨버터(전류제어모드 buck컨버더)형태의 여자시스템은 부하변동이 발생하였을 때 동기발전기의 계자전류를 제어하게 된다. 이 논문은 정상상태 및 과도상태에서의 안정화를 개선하기 위하여 여자시스템의 설계 및 실험에 대해 다루었다. 시뮬레이션 및 실험 결과는 제안된 여자시스템이 50kW 동기발전기의 DVR에 의해 응답시간이 개선되었음을 보여주었다. The output voltage of synchronous generator is regulated constantly by field current control in excitation system. High frequency PWM converter (current control mode buck conveter) type excitation system for synchronous generator is able to control exciter current when the load change happened. This paper deals with the design and evaluation of the excitation system for a synchronous generator to improve the steady state and transient stability. The simulation and experimental results show that the proposed excitation system is able to improve the response time by the DVR(digital voltage regulator) of 5O[kW] synchronous generator.