Efficient Interleukin-21 Production by Optimization of Codon and Signal Peptide in Chinese Hamster Ovarian Cells

( Hee Jun Cho ),( Byung Moo Oh ),( Jong-tae Kim ),( Jeewon Lim ),( Sang Yoon Park ),( Yo Sep Hwang ),( Kyoung Eun Baek ),( Bo-yeon Kim ),( Inpyo Choi ),( Hee Gu Lee ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.2

Interleukin-21 is a common γ-chain cytokine that controls the immune responses of B cells, T cells, and natural killer cells. Targeting IL-21 to strengthen the immune system is promising for the development of vaccines as well as anti-infection and anti-tumor therapies. However, the practical application of IL-21 is limited by the high production cost. In this study, we improved IL-21 production by codon optimization and selection of appropriate signal peptide in CHO-K1 cells. Codon-optimized or non-optimized human IL-21 was stably transfected into CHO-K1 cells. IL-21 expression was 10-fold higher for codon-optimized than non-optimized IL-21. We fused five different signal peptides to codon-optimized mature IL-21 and evaluated their effect on IL-21 production. The best result (a 3-fold increase) was obtained using a signal peptide derived from human azurocidin. Furthermore, codon-optimized IL-21 containing the azurocidin signal peptide promoted IFN-γ secretion and STAT3 phosphorylation in NK-92 cells similar to codon-optimized IL-21 containing original signal peptide. Collectively, these results indicate that codon optimization and azurocidin signal peptides provide an efficient approach for the high-level production of IL-21 as a biopharmaceutical.

대안정치성의 접합경쟁, 안철수 현상, 이정희 효과

조희연(Cho, Hee-Yeon),이창언(Chang-eon Lee) 비판사회학회 2013 경제와 사회 Vol.- No.97

필자는 2012년 대선결과를 출발점으로 하여 그것의 정치사회학적 의미와 박근혜 정부의 출현이 갖는 구조적 의미를 분석하고자 했다. 이를 위해 대선에서의 담론경쟁, 안철수 현상, 이정희 효과의 출현을 중심적인 분석대상으로 설정하였다. 먼저 2012년 대선은 이명박 정부의 사회경제적 실패로 조성된 ‘진보적의제 공간’에서의 경쟁이었다. 여기서 박근혜 후보는 반(反)복지세력으로서의 이미지를 벗어던지고 문재인 진영의 ‘전면적 복지’담론에 대항하는 ‘현실적 복지’담론으로 대중들의 저항적 정치성을 전유할 수 있었다. 다음으로 안철수 현상을 제도정치와 대중의 괴리에서 발생하는 대안정치성이 특정 인물에게 투사 되는 현상인데, 이것과 대선의 관계를 논하였다. 다음으로 ‘이정희 효과’를 ‘주류정당 간의 경쟁에서 급진진보세력이 세력이 갖는 정치적 효과’로 규정했는데, 2002년에는 이것이 ‘부정적인 위협효과’를 발휘하는 식으로 나타났다. 이는 급진진보정치세력의 ‘주변화’이자 ‘개혁적 국민정치와 계급정치의 분리’라고 하는 새로운 콘텍스트가 출현했기 때문이었다. 마지막으로 이명박 정부로부터의 대중의 이반에 의해서 강제된 ‘박근혜 진영의 온정적 보수로의 전환’을 제3수동혁명의 경향성을 갖는 것으로 규정했다. This article discusses the political and social implication of the presidential election in 2012 and structural meaning of emergence of Park Geun-Hye, based on its result. For this, I brought the discourse competition, Ahn Cheol-Soo phenomenon, and Lee Jung-Hee effect to the front. First, this election has been held in a kind of ‘political space’ bounded by progressive issues like welfare, economic reform, etc. In this space, the conservative candidate could appropriate the progressive and oppositive political demands with a discourse strategy of making a new image as a ‘realist welfare’ -oriented candidate against the ‘all-out radical welfare’ -oriented one. Second, I evaluated the presidential election by such a new concept as ‘the Ahn Cheol-Soo phenonenon’ was defined as projection of alternative political demands onto a certain outside-party figure, which comes from gap between the existing parties and the people. Third, Idiscussed the ‘Lee Jung-Hee effect’, which means the political effect of the radical candidate onto competition among mainstream candidates. This was expressed as ‘a negative threat effect’ rather than ‘a positive one’ demonstrated by Kwon Yeon-Gil candidate in the 1997 and 2002 election. This means a ‘marginalization of radical political force’, which came from a new context of ‘separation of reformative national politics and class politics.’ Before, the radical class political forces have been identified as a force to drive the reform of the national politics, despite radical. Finally, I defined transition of Park Geun-Hye camp to so-called ‘compassionate conservatism as having a potential of the third passive revolution’ preceded by the May 16 military coup and its developmental drive and June 29th Declaration coerced by the April 29 Student Revolution and June Democratic Struggle.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

2003년 국내 중증급성호흡기증후군 진료 현황 및 문제점 분석

이진수,김은실,정문현,백제중,정선화,안주희,최영화,이선희,고철우,김성범,김민자,박승철,기현균,송재훈,최상호,김양수,이상오,조용균,박영훈,정숙인,김연숙,이흥범,손창희,장성희,정희진,김우주 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 2002년 말 중국에서 SARS가 발생한 이후 국내에서도 2003년 10월까지 총 3명의 추정환자, 17명의 의심환자가 보고되었다. 향후 추가적인 SARS의 유행이 우려되는 상황에서, 그간의 SARS 환자 진료에 있어서의 실질적인 준비사항, 진료 현황 등에 대한 조사를 통해 문제점을 파악하여, 향후 더 나은 대비가 될 수 있도록 개선점을 제시하고자 하였다. 재료 및 방법 : SARS로 의심되는 환자를 진료 경험이 있는 병원의료진을 대상으로 2003년 10월에 설문조사를 실시하였다. 설문에는 SARS 환자 진료 시의 실질적인 조치, 진료 현황, 병실, 응급실 및 외래에서의 격리 시설과 준비사항, 보건당국의 관리와 지원에 관한 사항을 포함하였다. 결과 : 대상이 되는 22개 병원 중 17개(17/22, 77.2%) 병원이 설문에 응하였다. SARS 환자를 위한 격리실은 응급실, 외래, 일반병실 및 중환자실에서 각각 9개(9/17, 52.9%), 5개(5/17, 29.4%), 15개(15/16, 93.7%), 4개(4/16, 25%) 병원에서 음압처리가 되어있지 않은 일인실 혹은 다인실이 사용되었고, 1개(1/16, 6.3%) 병원에서만 일반병실에서 음압격리실이 운영되었다. 입원환자의 진찰 시 개인보호구의 착용은 거의 모든 의료기관에서 이루어졌다. 보건당국에서 SARS지정병원의 시설 등을 사전에 확인한 곳은 1곳(1/12, 8.3%)이였고, 14개 병원(14/15, 93.3%)에서는 보건당국에 의뢰한 검사결과를 통하 받지 못하였다. 결론 : 의료기관에서 SARS 환자용 격리실뿐만 아니라 기존의 격리실 설비 등이 미흡하였으며, 특히 중환자실 및 외래의 준비가 더욱 부족하였다. 보건당국의 의료기관에 대한 종합적인 지원이 부족하였고, 병원과의 원활한 연계가 잘 이루어지지 않았다. SARS 만이 아닌 격리를 필요로 하는 질환의 적절한 진료를 위해 향후 병원 시설의 정비와 정부차원에서의 보다 구체적이고 실질적인 대책마련이 필요하다. Background : There was an worldwide outbreak of the Severe Acute Respiratory Syndrome (SARS) originated from China in late 2002. During that period three cases of suspected SARS and 17 cases of probable SARS were reported in Korea. With the concerns about the reemergence of SARS-coV transmission, it is important to be prepared for any possibility. So, this study is aimed to analysis the past measures in managing SARS and propose the amendatory plans to improve the preparedness. Materials & Methods : Questionnaires were collected among clinicians with any experience in managing the probable or suspected SARS cases in Oct. 2003. 17 out of 22 hospitals responded to the questionnaire. The contents in the questionnaire were practical activities, personal equipments, response plans, isolation facilities in emergency centers, outpatient clinics, general wards and intensive care units, and relationship with the public health department. Results : The dedicated isolation rooms in emergency centers, outpatient clinics, general wards, and intensive care units were prepared in 9 (9/17, 52.9%), 5 (5/17, 29.4%), 15 (15/16, 93.7%), and 4 (4/16, 25.0%) hospitals, respectively. Except for one hospital that newly made negative pressure room for SARS, single or multi-bed rooms without airborne infection control were used in all the other hospitals. The personal precaution principles were kept quite well in general wards. Before the designation of SARS hospital by the public health department prior evalution to see if the hospital was suitable for managing SARS was conducted in only 1 (1/12, 8.3%) hospital. The results of laboratory diagnosis were reported back in 1 (1/15, 6.6%) hospital. Conclusions : The isolation facilities which can control airborne infection were almost deficient not only for SARS but also for other respiratory transmissible diseases. For the infection control of transmissible diseases including SARS, more investment is needed on medical facilities and comprehensive support from the public health department required.

건설공사의 재해예방에 관한 연구

연길환,조희제,임경택,윤석천 忠南大學校 産業技術硏究所 1994 산업기술연구논문집 Vol.9 No.2

This study aims to review the problems and status of accident and construction projects also to find out the ways of preventing the works from accident. It can let them understand the reasons for the accidents and the way of promoting the awareness of safety. For the study the literature and previous studies are reviewed and then the field managers and safety management members were interviewed intent of status of field safety management and accidents data occurred at domestic construction fields.

일반학교에 배치된 특수교육보조원의 역할수행 어려움에 대한 질적 연구

조연희,이숙정 국립특수교육원 2008 특수교육연구 Vol.15 No.2

특수교육보조원 제도가 본격적으로 시행되고 있지만, 당사자들이 교육현장에서 겪는 역할갈등, 어려움 등에 대한 논의는 피상적 수준에 머무르고 있다. 구체적인 교수학습 현장에 투입되어 나름의 교육활동을 하고 있는 이들이 느끼는 어려움에 대한 심층적 고찰은 이 제도가 제대로 정착하고 발전하기 위한 초석을 마련하리라 본다. 본 연구는 특수교육보조원의 역할수행 어려움을 당사자를 대상으로 반구조화된 질문지를 기준으로 3가지 측면에서 심층면담하였다. 교수-학습 지원 상황에서는 눈치, 역할지시의 부재로 인한 혼란, 지식의 부족으로 어려움, 인간관계에서는 무시, 역할에 대한 잘못된 인식, 차별적 관심과 대우, 어중간한 중간입장으로 어려움이 있었으며, 또한 환경적인 부분에서는 열악한 고용조건, 나만의 공간 부족, 부족한 연수, 불투명한 소속, 육체적 노동으로 인해 어려움을 느끼고 있는 것으로 나타났다. The teacher-aid-system in the field of special education has emerged as a developmental system for effective inclusion from March 2003 in South Korea. The role-conflict and difficulties of paraprofessional in special education have almost discussed in survey inquiry. But the subjective feeling about role-conflict of paraprofessional in the real field of teacher-aid-system could be different from the results through general survey inquiry. The purpose of this study was to analyze and interpretate qualitatively what are the role-conflict and bottlenecks of paraprofessional in the real field. They are asked with half-structured questionary in interview in depth. The results of this study were that: (a) in terms of personal relations in the school: the paraprofessional confronts with the problems like neglecting, wrong perception about their roles, discriminative treatment and position hanging in midair, (b) As concerns about situation for learning-support, they should often see how the winds blow between teacher and students. Furthermore they are diffused cause of insufficient instructions from the teachers, how the paraprofessional should support the student with special educational needs. In these case, they blushed at their ignorance. (c) About the working condition: they are suffering with much inconvenience like bad salary, poor working surrounding, lack of own-recreative space and hard-physical working.

한국의 경제성장과 정치변동 : '반공규율사회'와 '국가주의적 발전동원체제'의 형성, 균열, 위기 및 재편의 과정 Focused on the Formation, Disintegration, Crisis and Reformation of 'the Anticommunist Regimented Society' and 'the Statist Developmental Mobilization Regime'

조희연 성공회대학교 1999 성공회대학논총 Vol.- No.13

용혈성 빈혈과 흑수열을 동반한 Chloroquine저항성 수입 열대열 말라리아 1예

조희충,국기용,박일,최연수,이승일 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.1

저자들은 최근 아프리카를 여행하는 도중 말라리아의 예방약제인 chloroquine을 복용하였음에도 불구하고 용혈성 빈혈과 흑수열을 동반한 chloroquine 저항성 수입 열대열 말라리아를 Fansidar를 투여하여 치료가 된 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. This previously healthy 26 year-old student was admitted to Cho Sun university hospital due to paroxysmal high fever, dizziness of 2 weeks duration and he also noted passage of dark brownish colored urine of 3 days duration. He had made journey to Africa for 2 months before admission. He had taken chloroquine 1 week before his jurney and continued this weekly throughout his stay. On admission, there was fever of 38.5℃ and numerous parasitemia of ring form of P. falciparum in the peripheral blood smear. On physical examination, he had jaundice and splenomgaly. On laboratory examination, he had jaundice and splenomegaly. On laboratory examination hemoglobin was 5.Og / dl, reticulocytecount was 11%, plasma hemoglobin was 7.5mg /dl and urine hemoglobin was 2.5mg /dl. He received Fansidar on 2nd day of admission. Five days later, his blood was completely clear of parasite and 7th day after admission, all symptoms were almost improved and then he was discharged.

보육시설 과학 특별활동 프로그램의 개발과 적용

조복희,곽혜경,한유미,양연숙,손경화 한국영유아보육학회 2003 한국영유아보육학 Vol.0 No.32

The purpose of this study was to develop early childhood science activities, which could be utilized as an extracurricular program in the day-care center. The program is based on the Constructivist theory(Piaget and Vygotsky) and the nation-wide survey data on the extracurricular programs and science activities in the day-care center. After the manuals for the program was monitored and corrected by the professionals, the application of each activities was empirically tested by the teachers in the day-care setting. The effects of the sample program(6 activities) were conducted using Tegano, Sawyers & Moran(1989)'s Scientific problem-solving scale and Lee(2001)'s Scientific attitude scale. Subject of thirty nine children were administered by the pre-test and post-test and experimental group participated the program for six weeks. The results showed that the program was effective for enhancement of scientific problem-solving ability and scientific attitude of young children in day-care setting.

양파(Allium cepa L.) 추출물이 Tyrosinase 유전자 발현에 미치는 효과

조남철,윤연희,이혜진,손현정,김양경,최근희,나명석,조영권,이황희,진종언 한국식품영양학회 2001 韓國食品營養學會誌 Vol.14 No.3

Melanin 생성에 관여하는 tyrosinase 유전자의 발현을 억제하는 물질을 탐색하고자 tyrosinase promoter를 지닌 Bl6 mouse melanoma cell해 앙파 methanol 추출물을 처리한 바 양파 methanol 추풀물은 10.0㎍/ml, 100.0 ㎍/ml, 1.0 ㎎/ml의 농도에서 대조군에 비해서 약 15%, 23% 57%의 억제효과를 나타냈으며 세포생존율은 1.0 ㎍/ml, 10.0 ㎍/ml, I00.0 ㎍/ml, 1.0㎎/ml의 농도에서 약 126 %, 92%. 85%, 64%로서 세포독성이 낮게 나타났다. Ethyl acetate, butyl alcohol, 그리고 물 용매 분획물은 tyrosinase 유전자의 발현물 억제하는 효과가 없었지만 methylene chloride 용해 분획물은 10.0 ㎍/ml과 100.0 ㎍/ml의 농도에서 약 37%와 35%의 발현율을 나타냄으로서 대조군에 비해 크게 억제하였다. Onion(Allium cepa L,) extract by methanol repressed the expression of tyrosinase gene of B16 mouse melanoma cell containing tyrosinase promoter. 10.0 ㎍/ml, 100.0 ㎍/ml, 1.0 ㎎/ml of the extract repressed expression of tyrosinase gene about 15%, 23%, and 57%, respectively, compared with control. In the MTT assay, the same extract exhibited low cytotoxicity at 1.0 ㎍/ml, 10.0 ㎍/ml, 100.0 ㎍/ml, and 1.0 ㎎/ml, respectively. The fractions of ethyl acetate, butyl alcohol, and water did not showed the repressive effect on the expression of tyrosinase gene, but the fraction of methylene chloride repressed highly at 10.0 ㎍/ml and 100.0 ㎍/ml.