Complete genome sequence of Pseudomonas moraviensis EFBE32, a biocontrol bacterium against pepper bacterial wilt

안주희,김송화,상미경,진용주,김다연,김상윤,송재경 한국미생물학회 2022 미생물학회지 Vol.58 No.4

Pseudomonas moraviensis strain EFBE32 is a biocontrol bacterium against bacterial wilt which is known as most destructive disease caused by Ralstonia solanacearum. Here, we report the whole genome sequence of P. moraviensis strain EFBE32. The sequence analysis revealed that P. moraviensis strain EFBE32 has a single 6,030,129 bps circular chromosome with a DNA G + C-content of 60.1%. This chromosome contains 5,239 coding sequences and 16 rRNA and 69 tRNA genes. In the result of sequence analysis, it is revealed that strain EFBE32 possessed genes coding the disease suppression related enzymes, acyl-homoserine lactone acylase (QuiP and PvdQ) which is known as quorum quenching enzyme, and hydrogen cyanide synthase (HcnA, B, and C).

Inactivation Kinetics of Listeria innocua ATCC 33090 at Various Temperature Heating-up and Pressure Building-up Rates

안주희,V.M. Balasubramaniam 한국식품과학회 2007 Food Science and Biotechnology Vol.16 No.2

The effects of temperature heating-up rate and pressure building~up phase on the inactivation of Listeria innocua ATCC 33090 were evaluated in buffered peptone water. The number of 1. znnocua was reduced by 5.57 and 6.52 log CFUI mL during the nonisothermal treatment (the come-up time followed by isothermal process) and i?e Isothermal treatme~t, res ectivel at 60°C. When compared to the isothermal treatment (0.76<D value<0.9?), 1. znn~cua. exposed at t e no~sothe~al treatment (1.58<D value<2.31) became more resistant to heat. The come-up tune r~ductIons m numbers of 1. innocua significantly increased with increasing the heating rates (p<0.05). The pronounced ~eductI?n was observed by more than 5 log CFU/mL at 33.2°C/min of temperature heat!ng-~ate. T.he effect of the combmed high pressure and thermal processing on the inactivation of 1. innocua increased WIth mcreas~g pressure and temperature. ~t all temperature levels from 40 to 60°C under 700 MPa,1. innocua was not detected by ennchment culture (>7 log reduction).

Effect of a Post-packaging Pasteurization Process on Inactivation of a Listeria innocua Surrogate in Meat Products

안주희,이현용,Lynn Knipe,V.M. Balasubramaniam 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.5

Effects of surface pasteurization on inactivationof Listeria innocua were investigated. Surface temperature,monitored during post-packaging pasteurization, was usedto predict the lethality of L. monocytogenes. Temperaturesreached 70oC for lean and fat sausages within 9 min oftreatment. An inoculation study validated the efficacy ofpost-processing and the thermal lethality of L. monocytogenes. Pre-cooked sausage and ham, inoculated with approximately107 CFU/cm2 of L. innocua, were heated to a surfacetemperature of 70oC. Numbers of L. innocua were reducedby 7 log on surface-inoculated sausage. Guidelines for safe,ready-to-eat meat products are provided for small scalemeat processors.

Inactivation Kinetics and Injury Recovery of Bacillus amyloliquefaciens Spores in Low-acid Foods during Pressure-assisted Thermal Processing

안주희,Bala V. M. Balasubramaniam 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.6

Pressure-assisted thermal processing (PATP)inactivation kinetics of Bacillus amyloliquefaciens sporesin deionized water (DIW) were evaluated for egg pattymince (EPM) and green pea puree (GPP). Recovery ofPATP-injured spores during storage was determined. Thenumber of B. amyloliquefaciens spores in DIW wasreduced more than 6 log when treated at 121oC and 700MPa, including a come-up time reduction (3.32 log MPN/g) and a pressure holding time reduction (3.55 log MPN/g). Treatments at 700 MPa in combination with 105oC for16 min, 115oC for 5 min, or 121oC for 3 min, decreasedB. amyloliquefaciens spore populations in EPM to levelsundetectable using an enrichment procedure. No significant(p>0.05) recovery of PATP-injured spores was observed inEPM and GPP during storage for 8 weeks, compared withcontrols. These results provide useful information forenhancing microbial lethality of PATP-resistant bacterialspores in low-acid foods.

후기 근대사회 현대인의 불안과 강박에 대한 탐색 - 대학생을 중심으로

안주희 한국사회조사연구소 2009 사회연구 Vol.10 No.2

이 연구는 후기 근대화라는 문명사적 전환 속에서 새롭게 형성되고 있는 불안과 강박에 대해 탐색한다. 특히, 후기 근대로의 변화가 청년 세대의 불안을 어떻게 변화시키고 있으며, 새롭게 형성된 유형의 불안이 강박징후라는 특정 행동으로 어떻게 연결되는가에 주목하고자 한다. 다음과 같은 연구문제를 설정했다. 첫째, 후기 근대사회로의 변화에 대한 인식은 청년 세대의 불안에 어떠한 영향을 미치는가. 둘째, 불안이 강박적 징후에 미치는 영향은 어떠한가. 셋째, 불안에 영향을 미치는 후기 근대사회에 대한 인식뿐만 아니라 신뢰, 연구대상의 특정 요소가 불안을 매개로 강박적 징후에 어떠한 영향을 미치는가를 탐색하는 것이다. 이를 위해 대학생 431명을 대상으로 후기 근대에 대한 인식 및 신뢰, 불안감, 강박징후를 묻는 설문조사를 했다. 분석 결과를 요약하면 다음과 같다. 우선, 후기 근대에 대한 인식의 경우, 대학생들은 자신의 삶에서도 후기 근대로의 변화가 나타난다고 여겼으며, 연령이 낮아질수록 이에 대한 동의의 정도가 높았다. 둘째, 불안감 형성의 경우, 사회불안의 수준이 실존불안의 수준보다 전반적으로 높았지만, 실존불안의 경우 응답자들의 편차가 큰 것으로 나타났다. 마지막으로 연구문제로 설정된 주요 변수들 간의 관계를 정리해보면, 후기 근대인식의 정도가 높을수록 그리고 신뢰가 낮을수록, 불안감과 강박징후의 정도가 증가하는 것으로 나타났다. 또한 강박징후에 대한 불안감의 매개효과를 보면, 후기 근대에 대한 인식과 신뢰는 불안을 매개로 하여 강박징후에 영향을 미치는 것으로 나타났다.

영아기 사교육 유형별 실태 및 교사-부모 인식 비교

안주희,곽승주 한국열린유아교육학회 2017 열린유아교육연구 Vol.22 No.1

The purpose of this study was to grasp the private education status of infancy consequent on the private education type, and to look into the difference in perception of private education between teachers and parents. To this end, this study analyzed data targeting 235 parents with children aged between 0 and 36 months living in Gyeonggi-do Province, and 206 child care teachers with careers conducting infant classes. Results are as follows. First, special activities at day care centers were the most common type for infants, followed by private institute education, and home-visiting education in actual condition of experience by private education type. Also, it was found that private institute education and special activities at day care centers were developed with a focus on physical activity while home-visit education was developed with a focus on language and math activity for learning preparation. Second, results revealed a difference in private education between parents and teachers, namely that both parents and teachers perceived the private education of infancy as irrational exuberance; the reply ‘in order not to fall behind the same age group’ was found to be the highest. Also, as regards the private institute education among the private education types, teachers, much more than parents, felt that an infant’s participating period was much too early. As regards home-visiting education, both parents and teachers were found to perceive it as unsuitable for activity of infancy, whereas both parents and teachers perceived the special activity at a day care center as necessary. 본 연구는 사교육 유형에 따른 영아기 사교육 현황과 실태를 파악하고 사교육에 대한 교사와 부모와의 인식차이를 살펴보는 데 그 목적이 있다. 이를 위해 경기지역의 만 0~36개월 미만 자녀를 둔 부모 235명과 영아반 경력이 있는 보육교사 206명 총 441명을 대상으로 자료를 분석하였다. 연구결과 첫째, 사교육 유형별 경험 실태는 특별활동을 경험한 영아들이 가장 많았으며 다음으로 사설기관교육, 가정방문교육 순으로 나타났다. 사설기관 교육과 어린이집 특별활동은 신체활동을 위주로, 가정방문교육은 학습준비를 위한 한글․수 활동 중심으로 전개되고 있었다. 둘째, 사교육에 대한 부모와 교사의 인식차이를 분석한 결과, 영아기 사교육 대하여 부모와 교사 모두 과열현상이 있다고 인식하고 있었으며 그 이유로는 ‘또래보다 뒤떨어지지 않게 하기 위해서’라는 응답이 가장 높게 나타났다. 또한 사교육 유형 중 사설기관교육에 대해 부모보다 교사가 영아의 참여시기가 너무 빠르다고 인식하였다. 가정방문교육은 부모와 교사 모두 영아기 활동에는 적절하지 않으며, 어린이집 특별활동은 부모, 교사 모두 필요하다고 인식하였다.

나노분산 과불소계 술폰산 이오노머와 고분자전해질연료전지 및 수전해응용

안주희,임윤재,김우영,방준호,박종준,구영모,이창현 한국막학회 2019 한국막학회 총회 및 학술발표회 Vol.2019 No.05

친환경적인 술폰화 폴리아릴렌에테르술폰 공중합체 나노 분산막의 제조

안주희,이창현 한국막학회 2015 한국막학회 총회 및 학술발표회 Vol.2015 No.05

SPAE (Sulfonated poly(arylene ether sulfone), SPAES) copolymers exhibit a lot of advantages such as a relatively low production cost, and easy control of ionic conductivity and gas barrier properties, when they are used as membrane materials for polymer electrolyte fuel cells, redox flow batteries, and electrolysis. SPAES copolymer membranes are fabricated by evaporating polar aprotic solvents from their concentrated solutions. This conventional process, however, is needed to be excluded, owing to additional production cost associated with the organic solvent separation and hazardous issues. In this study, a benign membrane fabrication method is developed based on SPAES nanodispersions in water-alcohol mixtures. Their fundamental characteristics are systematically investigated in comparison with those made from the conventional method.

함침막 제조를 위한 술폰화 폴리아릴렌에테르술폰 공중합체-폴리에틸렌글리콜 소중합체 블랜드

안주희,이창현 한국막학회 2015 한국막학회 총회 및 학술발표회 Vol.2015 No.11

Sulfonated poly(arylene ether sulfone) (SPAES) random copolymers have been perceived as alternatives to perfluorinated sulfonic acid ionomers used as polymer electrolyte membranes for fuel cells. SPAES copolymers are suffering from degradation under harsh fuel cell operation conditions. One solution to overcome the decomposition issue is to fill SPAES copolymers into polymeric support films (e.g., poly(tetrafluoro ethylene), PTFE) with interconnected porous structures. It is difficult to fill the SPAES copolymers dissolved in polar aprotic solvents into PTFE support films owing to their different surface energies. In this study, a SPAES nanodispersion in a water-alcohol mixture is used to make defect-free pore-filling membranes where poly(ethylene glycol) oligomers are added to induce advanced morphologies for fast proton conduction.

라디칼에 대한 내화학성이 개선된 고분자 전해질 연료전지용 과불소계 술폰산 이오노머 막

안주희,임준현,이시찬,이창현,김주영 한국막학회 2022 한국막학회 총회 및 학술발표회 Vol.2022 No.11