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머신 러닝 기반 High Br 54SH Grade 영구자석 입계확산 최적 공정 도출 연구
임현석,이철희,나현민,배석,김동환 한국자기학회 2021 한국자기학회 학술연구발표회 논문개요집 Vol.31 No.1
새로운 연구 방법론인 머신 러닝 개발 기법을 활용하여 적은 실험 횟수로 영구 자석에 중희토 입계 확산을 통해 잔류 자기밀도 감소 최소화 및 보자력 증가를 극대화하여 높은 자기 특성을 도출하였을 뿐만 아니라 중희토 사용량을 최소화하여 세계최고 Br 특성을 갖는 54SH Grade 영구자석을 개발하였다. 개발된 영구자석을 이용하여 시뮬레이션 및 시제품 제작 검증 통해 모터 및 엑추에이터에 적용 높은 변환 효율 및 사이즈 축소 결과를 확인 하였다.
Schwanniomyces castellii CBS 2863(ATCC 26077)으로부터 $\alpha$-Amylase 정제 및 특성
박종천,배석,임선영,이진종,이향,전순배,Park, Jong-Chun,Bai, Suk,Lim, Suhn-Young,Lee, Jin-Jong,Lee, Hyang,Chun, Soon-Bai 한국미생물·생명공학회 1993 한국미생물·생명공학회지 Vol.21 No.6
The extracellular alpha-amylase was purified to homogenity from the culture filtrate of starch grown Sch. castellii CBS 2863. The purified enzyme was glycoprotein with a molecular weight of about 56 kDa. The pH and temperature optimum were 5.5 and 40C, respectively. The enzyme was fairly stable up to 40C and at acid pH range (pH 4.0-7.0). The apparent Km and Vmax of the enzyme toward starch was 1.0mg/ml and 100U/mg protein, respectively. The analysis of amino acid composition was found to be acidic protein. The amino acid sequence of N-terminal peptide consisted of Asp-Val-Ser-Ser-Ala-X-X-Thr-Arg-Ser-Glu-Ser-Ile-Tyr.
Schwanniomyces castellii CBS 2863(ATCC 26077)으로부터 α- Amylase 정제 및 특성
박종천,배석,임선영,이진종,이향,전순배 한국산업미생물학회 1993 한국미생물·생명공학회지 Vol.21 No.6
Schwanniomyces castellii CBS 2863의 배양 상징액으로부터 α-amylase를 정제하였다. Ultrafiltration과 gel filtration 두 단계를 통하여 정제된 α-amylase의 분자량은 약 56 KD이었고, glycoprotein이었다. 정제된 α-amylase 최적 pH와 온도는 각각 5.5와 40℃였고, pH 4.0∼7.0 범위와 40℃에서 안정하였다. 전분에 대한 K_m값과 V_max값은 각각 1.0 ㎎/㎖과 100 U/㎎ protein이었다. 그리고 아미노산 조성 분석결과 산성단백질이었고, N-말단 아미노산 서열은 Asp-Val-Ser-Ser-Ser-Ala-X-X-Thr-Arg-Ser-Glu-Ser-Ile-Tyr이었다. The extracellular α-amylase was purified to homogeneity from the culture filtrate of starch grown Sch. castellii CBS 2863. The purified enzyme was glycoprotein with a molecular weight of about 56 KDa. The pH and temperature optimum were 5.5 and 40℃, respectively. The enzyme was fairly stable up to 40℃ and at acid pH range(pH 4.0∼7.0). The apparent K_m and V_max of the enzyme toward starch was 1.0 ㎎/㎖ and 100 U/ ㎎ protein, respectively. The analysis of amino acid composition was found to be acidic protein. The amino acid sequence of N-terminal peptide consisted of Asp-Val-Ser-Ser-Ser-Ala-X-X-Thr-Arg-Ser-Glu-Ser-Ile-Tyr.
에디공분산 방법에 의한 GLDAS와 GLEAM 증발산량의 적정성 평가
이연길,임배석,김기영,이경훈 한국수자원학회 2020 한국수자원학회논문집 Vol.53 No.10
This study was performed in Seolmacheon basin to evaluate the adequacy of GLDAS (Global Land Data Assimilation System) and GLEAM (Global Land Evaporation Amsterdam Model) evapotranspiration data. The verification data necessary for the evaluation of adequacy were calculated after processing the latent heat flux data produced in the Seolmacheon basin with the Koflux program. In order to gap-fill the empty period, alternative evapotranspiration was calculated in three ways: FAO-PM (Food and Agriculture Organization- Penman Monteith), MDV (Mean Diurnal Variation) and Kalman Filter. This study selected Kalman Filter method as the data gap-filling method because it showed the best Bias and RMSE among the three methods. The amount of GLDAS spatial evapotranspiration was calculated as Noah (version 2.1) with a time interval of 3 hours and a spatial resolution of 0.25°. The amount of GLEAM spatial evapotranspiration was calculated using GLEAM (version 3.1a). This study evaluated the spatial evapotranspiration of GLDAS and GLEAM as the evapotranspiration based on eddy covariance. As a result of evaluation, GLDAS spatial evapotranspiration showed better results than GLEAM. Accordingly, in this study, the GLDAS method was proposed as a method for calculating the amount of spatial evapotranspiration in the Seolmacheon basin. 본 연구에서는 GLDAS (Global Land Data Assimilation System)와 GLEAM (Global Land Evaporation Amsterdam Model) 증발산량의 적정성을 평가하기 위해 설마천 유역에서 관측된 에디공분산 기반의 잠열 플럭스를 검증자료로 활용하였다. 잠열 플럭스로부터 증발산량을 산정하기 위해 Koflux 프로그램으로 자료처리하였으며, 자료처리 후 발생된 빈구간을 보충(Gap-filling)하기 위해 FAO-PM (Food and Agriculture Organization-Penman Monteith), 평균 일변동(Mean Diurnal Variation, MDV), 칼만 필터(Kalman Filter)의 3가지 방법으로 대체 증발산량을 산정하였다. 본 연구에서는 3가지 방법 중 칼만 필터(Kalman Filter) 기반의 증발산량이 우수한 Bias와 RMSE를 보여 자료보충 방법으로 채택하였다. 공간증발산량은 GLDAS의 경우 Noah (version 2.1, 3시간, 공간해상도 0.25°)로 추출하였으며 GLEAM의 경우는 GLEAM(version 3.1a, 1일, 공간해상도 0.25°)를 이용하였다. GLDAS와 GLEAM의 공간증발산량을 에디공분산 기반의 증발산량으로 적정성을 평가한 결과, GLDAS의 증발산량이 에디공분산 기반과 비교적 적정한 결과를 나타내었다.
Antitumor Effects of Adenovirus-RUNX3 on Human Lung Cancer Cells
박미영,어은영,임효정,박종선,조영재,윤호일,이재호,배석철,이춘택 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-
Introduction: Runt-domain transcription factor (RUNX3) has an important role in gene regulation in development. In several cancers, such as gastric cancer, RUNX3 showed tumor suppressive function. Frequent inactivation of RUNX3 by methylation also was found in lung cancer, especially adenocarcinoma. Interestingly, lung adenocarcinoma is observed in RUNX3 heterozygous mice. Recently, inactivation of RUNX3 draws an attention as a potential marker of tumor stem cell. Materials and Methods: We constructed a recombinant adenovirus expressing RUNX3 (ad-RUNX3) by homologous recombination and an adenovirus expressing RUNX3 dominant negative form (ad-RUNX3 DNF) as a nonfunctioning control adenovirus. We tested the function of these adenoviruses in several lung cancer cell lines. Results: Transduction of ad-RUNX3 in lung cancer cell lines showed the production of RUNX3 protein and the expression of RUNX3 was enhanced by vorinostat, histone deacetylase inhibitor which could increase the transduction and transcription of adenoviral transgene. Transduction of ad-RUNX3 showed moderate antitumor effect in lung cancer cell lines. However, combined treatment of ad-RUNX3 with vorinostat showed strong synergistic antitumor effect with enhanced induction of apoptosis. Conclusion: Restoration of RUNX3 via adenoviral vector demonstrated antitumor effect. Further study on ad-RUNX3 will elucidate the role of RUNX3 in lung tumorigenesis.
실측 수문자료를 기반으로 한 산지하천 유역의 물 수지 특성 분석
이연길 ( Yeon Gil Lee ),정성원 ( Sungwon Jung ),임배석 ( Baeseok Im ),윤양실 ( Yangsil Yun ),이경훈 ( Gyeonghun Lee ),김동필 ( Dong Phil Kim ) 한국수처리학회 2015 한국수처리학회지 Vol.23 No.5
This study estimates water budget characteristics based on observed hydrological data in mountainous river basin. We selected Seolmachoen test basin that is located in Han-river basin by study area. We considered water budget equation including four water elements to analysis water budget characteristics. This equation includes rainfall, runoff, evapotranspiration and storage variation. Four hydrological elements was quantified respectively by hydrological data observed for two years(2013~2014). We used daily rainfall and runoff data that were observed regularly by KICT(Korea Institute of Civil Engineering and Building Technology) in the Seolmachoen basin. We used latent flux observed in 20m flux tower with eddy covariance method to quantify daily evapotranspiration. And we gap-filled daily evapotranspiration data based on eddy covariance by three methods(FAO-PM, MDV and Kalman Filter). For calculation of storage variation, we used soil moisture content that is quantified to average individual soil moisture rate observed by TDR(Time Domain Reflectometry) sensor at soil depth. The soil moisture variation is calculated in consideration from initial to final soil moisture content. According to the result of this study, water budget is relatively well closed in 2013, but isn``t closed in 2014. Water budget error in years is calculated about 6.23%~6.83% of annual rainfall in 2013, but is calculated about 5.96%~20.49% in 2014. It is difficult to judge water budget error in Seolmachoen. We estimated soil moisture content as one of water budget errors, because soil moisture content didn``t represent actual storage variation.
해외 화산재해 법·제도 및 대응체계 분석을 통한 국내 화산재해 대응체계 구축 방안
박종석(Park, Jong Seok),이경훈(Rhee, Kyoung Hoon),이효범(Lee, Hyo Beom),임배석(Lim, Bae Seok) 한국방재학회 2018 한국방재학회지 Vol.18 No.4
지금까지 우리나라는 화산피해에 대한 직접적인 경험은 없기 때문에 백두산화산이 폭발하게 되어 대규모 분화가 일어난다면 준비가 안 된 국민들이 많은 피해를 보게 될 것이다. 이에 전조현상을 바탕으로 폭발을 예측하여 신속한 정보 전달과 방재교육으로 피해를 감소시키고, 우리나라에 맞는 실용적인 대응체계에 맞춰 대응하여 혼선 없는 대처를 할 수 있는 능력을 길러야 한다. 따라서 우리나라와 달리 화산분화와 피해가 자주 발생하는 일본, 미국, 인도네시아의 재난 대응 역할과 임무, 그리고 대응체계를 알아보고, 실제 화산분화에 따른 대처방법을 비교하여 우리나라에 적용 가능성에 대해 알아보고자 하였다. 연구 결과 우리나라의 화산방재의 가장 큰 문제점은 재난대해 대비체계에 대한 심각한 문제점이 노출 되었는데 그것은 재해를 늦게 감지하고 국민들에게 늦게 전파하는 것이다. 그리고 화산전문가가 배치되지 않아 실질적인 화산재해와 관련된 정책, 매뉴얼이 작성되지 않아 효율적인 방재효과가 날 수 없다는 점이다 Until now, our country doesn t have first hand experience about damage caused by volcanic eruption. So if Mt. Baekdu explode on a big scale, unprepared people will be damaged a lot. In order to reduce damage, we should anticipate explosion for prompt information delivery and disaster prevention education through precursor of eruption. We should develop abilities to handle the situation without any confusion with practical system of reaction which is suitable for our country. Therefore, we decided to inquire into the applicability of reaction system, investigating and comparing the role, duty, and reaction system of Japan, America, and Indonesia where volcanic eruption occurs frequently unlike our country. According to the result of research, the biggest problem of our country s volcanic eruption is about the reaction system for natural disaster. Our country detects disaster, and announce it too late. And efficient prevention effect can t be seen as there are no proper policy and manual because there is no volcano experts.