축소 모형을 이용한 열분해 용융소각로용 기포식 용탕의 열전달 특성에 관한 실험적 연구

신동훈,류태우,조홍제,이진호 한국폐기물자원순환학회 2006 한국폐기물자원순환학회지 Vol.23 No.1

일부 6가 크롬 폭로 작업자의 임파구 자매염색분체교환

신동훈,윤능기,서석권,예민해,Shin, Dong-Hoon,Yoon, Nung-Ki,Suh, Suk-Kwon,Yeh, Min-Hae 대한예방의학회 1990 예방의학회지 Vol.23 No.3

1989년 11월부터 4개월간 대구시내 소재 일부, 6가 크롬(삼산화크롬)으로 피막처리하는 도금작업장의 남성근로자를 대상으로 비중격천공이 있는 근로자 12명과 비중격천공이 없는 근로자 12명을 폭로군으로 하고 크롬에 폭로된 적이 없는 정상인 20명을 대조군으로 하여 개별면담을 통해 연령, 직업력, 흡연습관을 파악하였으며, 정맥혈을 채취하여 원자화 무염광로를 부착한 원자흡광도계로 혈중 크롬농도를 측정하였으며 말초혈액 중 임파구의 자매염색분체교환의 발현빈도를 조사하고 연령, 흡연습관, 직업력 및 혈중 크롬농도 등이 임파구 자매염색분체교환의 발현빈도에 미치는 영향을 조사했다. 크롬 도금작업의 말초혈액 중 임파구 자매염색분체교환의 발현빈도는 비중격 천공이 있는 군이 $10.1{\pm}1.6$, 비중격천공이 없는 군이 $8.5{\pm}3.4$이었고 대조군 $6.1{\pm}2.1$으로 세군간에 유의한 차이가 있었으며 (p<0.01), 비중격천공 군에서 가장 높은 빈도를 보였으며, 대조군에 비해 1.7배의 높은 빈도를 나타냈다. 혈중 크롬농도는 대조군이 $0.060{\pm}0.043{\mu}g/ml$, 크롬도금 작업자 중 비중격천공이 있는 군 $0.114{\pm}0.030{\mu}g/ml$, 비중격천공이 없는 군 $0.090{\pm}0.050{\mu}g/ml$로 세군간의 차이는 유의하였다 (p<0.01). 크롬도금 작업자와 대조군 모두 연령군별, 흡연습관별 및 근속년수에 따른 자매염색분체교환의 발현빈도에는 유의한 차이가 없었다. 혈중 크롬농도와 자매염색분체교환 발현빈도의 상관성은 상관계수, r=0.007로서 상관관계가 없었다 (Y=0.638 X+0.191, r=0.007. p>0.05). SCE 평균 발현빈도를 $y=(sum\;SCE)^{\frac{1}{2}}+(sum\;SCE+1)^{\frac{1}{2}}$를 이용하여 변형한 변수 y에 대해 연령, 흡연습관, 직업 및 혈중 크롬농도의 요인들로서 설명력은 32.3%였으며 가장 중요한 변수는 직업으로 나타났다. 이상의 결과로 보아 직업적인 크롬폭로에 의한 유전적 손상의 위험성이 어느 정토 있을 것으로 추측되며 생물학적 독성검정 지표로서 유용성이 있을 것으로 생각된다. To investigate the possibility of utilizing of sister chromatid exchange(SCE) analysis in lymphocytes as an indicator which could evaluate the effects of mutagenicity after in vivo exposure to hexavalent chromium, this study was conducted using some of chromium plating workers occupationally exposed to hexavalent chromium, chromium trioxide ($CrO_3$) in Taegu city. The study population was 12 Cr platers with perforation of nasal septum, 12 Cr platers without perforation of nasal septum and 20 controls. The SCE in peripheral blood lymphocytes of the subjects was analyzed and blood chromium concentration was estimated using the atomic absorption spectrophotometer (IL551) equipped with furnace atomizer (IL755). The mean SCE frequencies for Cr platers with and without perforation of nasal septum were statistically higher than those for control. The difference in SCE frequencies by age, smoking habits were not statistically significant both in Cr platers and controls. There was no difference in SCE frequencies by career of Cr platers workers. In Cr platers, the correlation between the mean SCE frequencies and chromium concentration in blood was not statistically significant. Using the transformation $y=(sum\;SCE)^{\frac{1}{2}}+(sum\;SCE+1)^{\frac{1}{2}}$, when the data was studied by multiple regression, it appeared that the influence of the occupation was the most important. Age, smoking, occupation and CrB(blood chromium concentration) together explain only 32.3% of interpersonal variation on SCE. The results in this study suggest tt a genetic risk due to occupationally exposure to hexavalent chromium is clearly inferable and thus, SCE analysis in human lymphocytes may be used indicator of biological toxic effects of chromium. Further, populatio analysis stuies are required before SCE frequency can be used as a mutagenic indicator in human population.

대뇌 기저핵 신경세포 일차배양에서 망간에 의해 유도된 Appoptosis : 형태학적인 소견

신동훈,김상표,정용욱,배재훈,송대규,백원기 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.1

목적 : 본 실험은 대뇌기저핵의 신경세포를 배양하여 망간(MnCl₂)을 투여한 후 망간독성에 의한 신경세포의 apoptosis를 형태학적인 소견으로 관찰하였다. 방법 : 배양된 신경세포에 0.01에서 10μM Mnc12를 48시간동안 처리한 후 TUNEL (TdT-mediated dUTP Nick End Labelling)법 및 투과전자현미경학적으로 관찰하였다. 결과 : TUNEL방법을 이용하여 관찰한 결과 TUNEL반응에 갈색으로 양성반응을 나타내는apoptotic 세포의 수가 대조군에 비해 MnCl₂를 투여한 군에서 유의하게 높게 나타났으며(P〈0.05), 투과전자현미경학적 소견상 대조군의 신경세포들은 핵인(nucleolus)이 두드러지게 특징적으로 보이면서 핵막과 세포질내 소기관들이 잘 보존되어 있으며, 세포질내망(ER)과 사립체(mitochondria)를 특히 많이 가지고 있었다. Mnc12를 48시간 동안 처리한 군에서 이질염색질(heterochromatin)이 핵막으로 이동하면서 응집되어 있었으며, 핵내 불규칙한 형태의 염색질이 나타나 분절이 진행되는 소견을 보였고, Apoptosis의 가장 특징적인 초기 소견인 막으로 둘러싸인 반달모양의 핵내염색질의 분절편(fragment-ed chromatin)과 주위의 상대적으로 정상적인 소기관으로 구성된 apoptotic body를 관찰할 수 있었다. 결론 : 신경세포에서 망간에 의해 apoptosis가 유도됨을 형태학적인 방법으로 확인할 수 있었으며 망간에 의한 세포사망양상에 apoptosis가 하나의 기전이 될 수 있을 것이다. Objectives : Manganese is cytotoxic to the central nervous system including basal gan-glia. Its toxic mechanism is related to oxidative stress, mediated by toxic free radicals but is specultives. In the present study , we have investigated to manifest apoptosis in man-ganese-induced cytotoxicity in primary neuronal cell culture of rat basal ganglia. Methods : To detect apoptotic neuronal cells were stained by the terminal deoxynu-cleotide (TdT) -mediated dUTP nick end-labelling (TUNEL) method and apoptotic changesin nuclei of neurons were observed by electron microscopy. Results : We showed that TUNEL immunostain showed brownish signal in the nuclei of apoptotic cells and the proportions of apoptotic cells in Manganese treatment groups were more higher than controls. On transmission electron microscopy, there were chromatine condensation with margination toward nuclear membrane and condensation of cyto-plasm in the treated with luM MnCl²for 48 hours in a basal ganglia neurons. Apoptotic bodies were found and consisted of semilunar-like condensed nuclei with relatively intact cytoplasmic organelles. Conclusions : Apoptosis appears to be one mechanism in the manganese-induced net-ronal cell death. Manganese intoxication is a convenient model for apoptosis study.

Solution Processed Discontinuous MoO₃ Hole Transport Layer for Organic Solar Cells

신동훈,조길원,조새벽,김민,김주현,고효민 한국고분자학회 2014 한국고분자학회 학술대회 연구논문 초록집 Vol.2014 No.4

도시하천경관복원 접근방법에 관한 고찰- 청계천 복원사업을 사례로 -

신동훈,이규석,Shin Dong-Hoon,Lee Kyoo-Seock 한국조경학회 2004 한국조경학회지 Vol.32 No.4

In the 1960s, polluted urban streams were covered and paved in concrete. Rivers became sewers buried under road. As a result, wildlife habitat was lost and the open stream space was gone. In the 1990s, there was a movement for restoring urban streams to enhance the quality of life of inhabitants. Chungkye Stream, whose covering began from early 1950s and ended in 1977, is now in the process of restoration together with the Central Business District (CBD) Redevelopment Plan. However, as the construction proceeds, several problems were raised, and serious controversies resulted. Therefore, this study aims to investigate the validity of the approach of the Chungkye Stream Restoration Project through a literature survey and a site survey, and to present the improved alternative. Ultimately, the study aims to contribute to present references or guidelines for similar urban stream restoration projects. The Chungkye Stream Restoration Project has some of the following problems. First, the construction should be done based on the long-term urban redevelopment plan. Second, the construction period should be extended to restore urban streams. Third, the cultural asset conservation plan should be implemented. In order to fulfill the anticipated purpose, the above mentioned problems need to be solved.

Iron oxide/carbon hybrid nanoparticles for detection of NO₂ gas

신동훈,장정식 한국공업화학회 2014 한국공업화학회 연구논문 초록집 Vol.2014 No.1

The Fe₂O₃/Carbon hybrid nanoparticles (NPs) were fabricated by using dual-nozzle electrospray, heat stirring and carbonization. According to the increased diameter of polypyrrole (PPy) NPs as core parts, the size of hybrid NPs also increased gradually. Furthermore, the Brunauer-Emmett-Teller (BET) surface area was decreased, exponentially. This is because the additional pore in carbonization steps. These synthesized hybrid NPs were applied to detect the NO₂ gas at room temperature. The minimum detectable level (MDL) of hybrid NPs was as low as 1 ppm, which is higher than that for a chemical sensor based on hybrid materials.

Fabricationof ulchin-like Pd particles decorated graphene for H₂ gas sensor

신동훈,장정식 한국공업화학회 2014 한국공업화학회 연구논문 초록집 Vol.2014 No.1

The Ulchin-like palladium (Pd) nanoparticles (NPs) decorated graphene werefabricated by using Chmical Vapor deposition (CVD) and Electro-deposition. Usingthe different concentration of 1,5-diaminonaphthalene (DAN), the population ofPd NPs can be controlled. These synthesized Pd NPs decorated graphene wasapplied to the hydrogen (H2) gas sensing electrode at room temperature. Theminimum detectable level (MDL) of Pd NPs decorated graphene was as low as 1 ppm, which is higher than that for a chemical sensor based on hybrid materials.

자연형 하천공사 후 도시하천의 식물상 변화

신동훈,노태성,오휘영,이규석 한국조경학회 2003 한국조경학회지 Vol.31 No.4

Natural stream work has been implemented recently to improve the urban stream environment in Korean cities. Many plants planted in the early work have not survived because they were not suited to the site and therefore failed to adapt to the riparian environment. The objective of this study is to investigate the floral change at the riparian environment after completion of the natural stream work by comparing the differences between planted species during the work and the current species at the study site. The study site was the stream area between Yeongdohng 2 bridge and Yeongdohng 3 bridge at Yangjae-cheon Stream, Gangnam-ku, Seoul, which was the prototype site of G-7 project of the Ministry of Environment in Korea. The following conclusions were derived after doing this study: Among the 50 species planted during the work, 23 species survived in the stream zone, while 27 species did not. The species that did not survive were ornamental plants that were not appropriate for the riparian environment. Among the 144 species which appeared naturally, 77 species(53.5%) were annuals and perennials, which means the study site is still in ecological disturbance and does not have a stable status ecologically. Thus, it is necessary to select the plant species that can survive in the disturbed riparian environment.

2024 다문화청소년 종단연구: 총괄보고서

신동훈,김희진,이정민,이용해,장윤선 한국청소년정책연구원 2024 한국청소년정책연구원 연구보고서 Vol.2024 No.-

2024 다문화청소년 종단연구: 기초분석보고서(2기 패널)

신동훈,김희진,이정민,이용해 한국청소년정책연구원 2024 한국청소년정책연구원 연구보고서 Vol.2024 No.-