Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

담낭 방선균증 1예

김형수,정진원,김양수,우준희,송현순,류지소,김명환,김남중 대한감염학회 2003 감염과 화학요법 Vol.35 No.5

저자들은 우상복부 통증과 소화불량을 주소로 내원한 83세 여자 환자에서 국내 최초로 담낭 방선균증을 진단하고 치료 관찰 중으로 이에 문헌고찰과 함께 보고하는 바이다. Actinomycosis is a chronic suppurative and granulomatous disease, characterized by abscess formation, tissue fibrosis, and draining sinuses. Actinomycosis of the gallbladder is very rare and has not yet been reported in Korea. We report a case of actinomycosis of the gallbladder in an 80-year-old women with history of cholelithiasis for 3 years, preoperatively misidentified as gallbladder cancer. Histologic section of the gallbladder showed Gram-positive branching bacilli compatible with actinomyces. After cholecystectomy, the patient received intravenous ampicillin for 4 days, followed by amoxicillin for 4 months thereafter.

소에서 盲腸擴張을 동반한 第4胃食滯 手術 1例

김명철,김덕환,신상태,임준호,김남중,김종만 충남대학교 수의과대학 동물의과학연구소 1998 動物醫科學硏究誌 Vol.6 No.-

Abomasal impaction with cecal dilatation was diagnosed in a 3 years old holstein cow. The clinical signs were loss of appetite, scant feces and moderate distension of the abdomen. Ping sound was revealed in the right 11th intercostal space by auscultation. Cecal dilatation and abomasal impaction were ascertained by right flank laparotomy and abdominal exploration, and intestinal content and gas were removed from the cecal apex incised. One day later, 2.5㎏ of sand was removed by ventral paramedian abomasotomy.

소규모사업장 용접근로자들의 소음·용접흄·망간 노출에 관한 연구

김동구,문덕환,이채관,박명희,함성애,손병철,김대환,이창희,김휘동,이채언 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.5

Objective : This study was conducted for the purpose of investigating the level exposure of welders to noise, welding fume and Manganese(Mn) related to this process. Methods and Material : All subjects in this study were welders engaged in CO2 arc and coated arc welding process especially in small-scale enterprises classified by type of manufacture of ship building, repairing and of other fabricated metal products, which is located in Gimhae and Milyang of Kyeongsang province in south Korea. Air sampling of welding fume was measured, shipped and analyzed on the basis of national institute for occupational safety and health(NIOSH) method in U.S. and work environment measurement method established in south Korea. To measure Noise in the work environment, Noise dosimeter was used on the basis of work environment measurement method established in south Korea. Result : This study was performed during a period from January, 2000 to December, 2001 and Results were as follows: 1. Noise level measured in this work environment was higher than national criteria for permissible exposure limit on health examinations related to hearing loss. 2. The concentration of welding fume and Mn in manufacture of ship building and repairing was higher than those in manufacture of other fabricated metal products. 3. In conditions of existing in ventilation system installed and operated, the concentration of welding fume and Mn was slightly lower than those of not existing. 4. In conditions of existing in sufficient air supply from out door into the work place relied on natural exhaust system, the concentration of welding fume and Mn was slightly lower than those of in not sufficient. 5. In all subjects, a rate of wearing ear plug and respiratory mask for dust and fume was lower than that of not wearing. Conclusion : According to above results, this study suggests that more positive work environment managements such as the use of portable local ventilation system, the supply of highly qualified personal protective equipment, and consecutive education and training related to health protection should be need to acquire suitable health protection program for welders engaged in small-scale enterprises in Korea.

구내 골신장술 후 식립된 전치부 임플란트보철 치험보고

김한석,김명래,이성근,강상원,김기환,김형섭 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.1

Objective : This is to report the effectiveness of intraoral distraction osteogenesis for alveolar augmentation in the extremely atrophied alveolar defects and to review the clinical results of the 2 cases. Subjects and Methods : Anterior segmental osteotomy was performed and the trans-oral alveolar distractors(Martin, Germany) were applied in 2 patients with the severe acquired anterior maxillary and mandibular defect. After latent period for 1 week, the osteomized alveolar segments were distracted by 1 ㎜ a day (0.5 ㎜/l turn) for 8 days in case Ⅰ and 0.6 ㎜ a day(0.3 ㎜/l turn) for 10 days in case Ⅱ. The consolidation period was about 8 weeks in each case. Thereafter, 6 titanium threaded implants(Branemark, Restore) were simultaneously installed with removal of distractor. The implants were tested clinically and radiographically. Results : Amounts of acquired alveolar bone were 8 ㎜ in case Ⅰ and 6 ㎜ in case Ⅱ respectively, with the increased width of the ridge crests and soft tissue expansion. Dental implants installated on the augmented alveolar ridges in 8 weeks after distrction were confirmed as in good osseointegration and in good function without any complications. Conclusion : Intraoral distraction osteogenesis can be a good option for alveolar ridge augmentation of the severely atrophied ridges and soft-tissue defects. However, for the better vector control of distracting segments and for anterior esthetics, customized distraction devices could be considered later.

면양에서 개량된 제1위 누관 형성술 1례

김명철,김종만,김용국,김덕환,이영원 충남대학교 수의과대학 동물의과학연구소 1999 動物醫科學硏究誌 Vol.7 No.-

We performed rumen fistula operation in a sheep. In sheep, the procedure for a rumen fistula is quite successful, but choice of an appropriate cannula is of prime importance. The sheep was anesthetized by xylazine(0.1 ㎎/㎏ IM) administration 5 minutes after administration of atropine sulfate(0.1 ㎎/㎏ IM). We inserted rubber cannula in rumen incised and put rubber ring after employing purse-string suture in rumen. We marked the area for the puncture incision in the body wall through which the cannula is drawn. We drew the cannula as near the transverse process of the lumbar vertebrae as possible to minimize leakage. We isolated the cannula by fixing the stainless washer which has small holes. The sheep was given postoperative penicillin(20,000 IU/㎏ IM) to prevent secondary infection for 3 days. There were not observed the leakage of rumen contents, the entering of rubber cannula into abdominal cavity, and necrosis on incision site and skin, over months after surgery.

간세포암환자에서 간동맥 화학 색전술 후 발생한 리피오돌에 의한 폐렴 1예

김소이,김유리,허현미,배서은,이명원,최윤정,김고흔,김태헌,유 권,문일환 이화여자대학교 의과대학 2009 EMJ (Ewha medical journal) Vol.32 No.2

Hepatocellular carcinoma(HCC)is one of common causes of cancer-related death in Korea where the majority of HCC patients were Hepaitc B virus(HBV)carriers and have cirrhosis. Transarterial chemoembolization(TACE)is commonly applied to the treatment of multinodular HCC in Korea and careful selection of candidate is important for the risk of various side effects. Besides common side effects as fever, nausea, abdominal pain and elevation of liver enzyme, TACE may predispose to hepatic failure, ischemic cholecystitis, pulmonary embolism, cerebral embolism and pneumonitis. In previous studies, some cases of pulmonary and cerebral embolism cases were reported but lipiodol pneumonitis after TACE was rarely reported. A 65-year-old woman with a multinodular HCC associated with HBV infection, was treated with TACE. Seven days after the procedure, nonspecific respiratory symptoms such as dyspnea and dry cough developed. Chest X-ray and chest computed tomography showed diffuse ground glass opacities in whole lung fields, suggestive of lipiodol pneumonitis. After several days of supportive care with steroid administration, radiologic abnormalities and subjective symptoms were much improved, considered that the disease was compatible with lipiodol pneumonitis.

초등학교 자연과 교육과정안의 작성과 그 연구

임정환,김병업,김명환,김재경 대구교육대학교 1970 論文集 Vol.6 No.-

This researching group of four members of us organized this test plan on the ground of following standpoints: 1. Science education should be designed for the general outline of natural science to be taught till the period of compulsory education is expired. 2. Natural science consists of two elements; one is the scientific method as a way of understanding nature and the other is the system of concept which is got as the result. 3. Science education should be designed, after increasing the belief in sciecne, for the effects of learning to be transformed into the sources of the judgements and behaviours. In this test plan, we definitely represented the above three standpoints as followings: 1. These system of concept of natural science can be condensed into several basic general concepts. These basic concepts become the essence of curriculum plan. Field of Non-living Matters Field of Living Thing 1. Matter 1. Feed 2. Force and Motion 2. Growth 3. Energy 3. Reproduction 4. Time and Space 4. Interaction to Environments 2. The general conepts are incessantly extended and deepened through the process while the scientific methods are applied. The scientific methods which are applied here are these: 1) Observation 2) Classification 3) Experiment 4) Modeling 3. The contents of the thextbook were chosen all over the fields of the natural science but the various contents should be taught wholly and connectively all over the nature. We settled the syllabus between the first grade and the sixth of elementary school on the ground of the above-mentioned basic standpoints and principles.(Refer attached table.) When this test plan is organized successfully, a desirable character who can flexibly adapt to the rapidly changing society and has extentive possibilities will be expected through science education.

The Effect of Font Type and Font Size on the Legibility in Smart Watch Usage of the Elderly People

Na Kyoung You,Minjee Kim,Chunyan Shan,SungYub Kim,Jungyoon Song,Sangmin Yeo,Donghyun An,Myung Hwan Yun 대한인간공학회 2017 대한인간공학회 학술대회논문집 Vol.2017 No.11

Objective: The aim of this study is to investigate the effect of character size and font type on legibility of elderly in smart watch usage, and to suggest a design guideline for smart watch - dedicated for legibility enhancement of the elderly people. Background: As the elderly population increases, the demand for health care and relevant costs for elderly are increasing correspondingly. Hence, there have been researches to implement wearable device onto the health monitoring system for elderly. In such manner, smart watch is one of the wearables popularly studied. However, poor legibility due to the small display of smart watch is considered to be a problem for the elderly to fully use it. Thus, legibility should be improved to allow elderly to use the smart watch more conveniently. Method: The task of selecting a target stimulus within two simultaneously given stimuli on a smart watch display was given to participants. A total of 10 sets of stimuli was given, and they were in combination of single font size and type. All subjects performed the task for a total of 8 stimuli sets combining 4 font sizes and 2 font types. The dependent variable is the time to select stimulus and the error occurrence frequency: the number of interference stimulus chosen. Results and Conclusions: In Gothic type, there was no significant differences in recognition time between font sizes. However, in the case of Myung, 9pt, 10pt is better than 11pt, and 12pt. In the case of error frequency, it is shown that 10pt and 11pt results better compared to other two font sizes in case of Gothic. There was no difference in Myung. Application: The results of the study can be the useful guideline to improve legibility for the elderly.