토양으로부터 分離한 Bacteriophage의 특성

박현균,김도경,박경진,전홍성,박종필,김종승,김성준 조선대학교 부설 유전생물공학연구소 1992 유전생물공학연구지 Vol.2 No.-

환경 미생물 분야와 산업미생물 분야 및 분자생물학 분야에서 주목을 받고 있고, 많은 연구가 된 새로운 E. coli의 bacteriophage를 토양으로 부터 분리해서, 그 물리적인 특성과 핵산을 밝혀내, phage상호간의 관련성을 검토하여 보았다. 그 결과를 요약하면 다음과 같다. 1. 토양으로 부터 분리한 phage는 숙주균에 따라 SJ-1, SJ-2, SJ-3, SJ-4, SJ-5, SJ-6, SJ-7이라 명명하였다. SJ phage들은 높은 숙주특이성을 나타내 phage SJ-2, SJ一7은 단일종 숙주균에만 감염이 되었고, SJ-1, SJ-3, SJ-4, SJ-5, SJ-6은 다중독성을 나타내었다. 2. 7종류의 SJ-phage는 본 실험조건하에서는 모두 virulent phage임이 밝혀졌다. 모든 용균반의 형태는 중앙이 투명한 둥근 모양이었으며 주변에 무리가 있는 것과 없는것 두가지 형태를 나타냈다. 용균반의 크기는 1-5mm이었으며, SJ-5phage의 용균반이 5mm로서 가장 컸고 SJ-7 phage가 가장 적었다. 숙주균은 OD_600 0.3-0.5상태에서 가장 감염되기가 좋았다. 그리고 상층배지의 한천 농도와 양은 0.5%와 3ml이 가장 좋은 조건이었다. 3. 일단 증식 실험의 결과를 보면 SJ-phage는 잠복기가 15-50분이었고 평균 phage방출수는 190-250이었다. SJ-6과 SJ-7이 잠복기가 15분으로 가장 짧았고 SJ-4가 50분으로 가장 길었다. 평균 방출수는 SJ-3과 SJ-5가 250정도로 많았으며 SJ-1과 SJ-2는 약 190정도였다. 4. SJ-phage는 PD buffer에서 안정하였고, magnesium ion 5×10^-3M의 첨가는 phage에게 큰 안정성을 주었다. SJ-phage는 pH6과 pH9사이에서 안정하였으며 그 pH는 숙주균의 최적 pH였다. SJ-phage의 온도 불활성화는 40 ℃이상에서 불활성화되기 시작하여 60 ℃이상에서는 완전히 불활성화 되었다. 자외선 조사에 의한 불활성화에서 SJ-phage는 120초 이후에 완전히 불활성화 되었다. All phages used in this experiment were isolated from soil of the surburbs of Chun-Nam, and they designated as phage SJ-1, SJ-2, SJ-3, SJ-4, SJ-5, SJ-6, SJ-7. The hosts were JM105, JM109, K802, LE392, MC1000, MV1184 and PSM CC, respectively. These phages were virulent under the experimental conditions. They produced plaques with dear and round without halo. The size of plaques was 1-5mm in diameter. SJ-phages were stable in 5×10^-3M Mg^2+. Thermostability experiments indicated that SJ-phages were stable at 37℃. Optimal pH of the phages were 6-9. Exposure of the phages to U.V. for 120 seconds resulted in complete inactivation. In one-step growth experiments, the latent period at 37 ℃ was about 15-50 min and the average burst see was 190-250. Agarose gel electrophoresis of the phage DNA showed single band of high molecular weight from SJ-1 to SJ-7 phages

엔로플록사신 액제의 닭유래 병원세균에 대한 시험관내 항균효과 시험

윤효인,박병권,조준형,정상희,박경환,장범수,김종춘,황윤환,임종환,이내경,박승춘 충남대학교 수의과대학 동물의과학연구소 2000 動物醫科學硏究誌 Vol.8 No.-

In this study the antimicrobial spectrum and sensitivity of a liquid formulation of enrofloxacin against the major six pathogenic bacteria isolated from the diseased poultry in Korea were evaluated in comparison with ciprfloxacin (CFX), gentamicin (GM) and penicillin (PC). A liquid form of enrofloxacin, a test material and ciprofloxacin showed at least 83% antibacterial activity against all of six test pathogenic microorganisms at the fixed concentration of 10 ㎍/㎖. Gentamicin and penicillin revealed much lower antibacterial activity at the same condition. We evaluated the liquid form of enrofloxacin as having widespread antibacterial spectrum against poultry infections. The liquid form of enrofloxacin showed potent antibacterial activity, with the MIC range being 0.12∼2 ㎍/㎖ against Gram positives, 0.06∼2 ㎍/㎖ against Gram negatives and 0.5∼2 ㎍/㎖ against Mycoplasma spp. These values were equivalent to or somewhat higher than those of ciprofloxacin, but much lower than those of gentamicn and penicillin. This new liquid form of enrofloxacin would find its way in application of mixing with drinking water for the prevention and treatment of diverse infections in chickens.

엔로플록사신 액제의 닭 호흡기 및 소화기 감염증에 대한 예방 및 치료효과

윤효인,박병권,조준형,정상희,박경환,장범수,김종춘,황윤환,임종환,이내경,박승춘 충남대학교 수의과대학 동물의과학연구소 2000 動物醫科學硏究誌 Vol.8 No.-

We evaluated the efficacy of the liquid form of enrofloxacin against poultry respiratory and digestive infections, the major problems in the poultry industry, from diverse diagnosis viewpoints. Results obtained through this study were as follows: 1. The liquid form of enrofloxacin showed good acceptability to poultry owing to no abhorrence to the drug by chickens, which indicated its suitability for mixing in drinking water. 2. The application of the enrofloxacin formulation improved the productivity of the chicken farm to a great extent by lowering the death toll of chicken raised in the farm. 3. The use of the drug shortened the days to the market, and increased the body weight at the time of marketing. 4. Preventive and therapeutic effects of the liquid enrofloxacin were observed higher than ampicillin, a major antibiotic used in poultry farming. From above results, the liquid form of enrofloxacin would find its use for the prevention and treatment of digestive and respiratory infections, let alone the growth promotion and feed efficiency, especially in the chicken farming practice.

40세 이하 관상동맥 질환자에서 장기적 임상 관찰

이승욱,정명호,박종철,박형욱,조상희,김원,김계훈,이상현,강경태,조장현,김남호,김건형,안영근,조정관,박종춘,강정채 全南大醫大 2001 전남의대학술지 Vol.37 No.2

보문 : Polyethylene Film포장이 포도 Sheridan의 저장력에 미치는 영향

남상영(Sang Young Nam),김경미(Kyoung Mi Kim),박종천(Jong Chun Park),주선종(Seon Jong Joo),정재현(Jai Hyun Jung) 한국식품저장유통학회(구 한국농산물저장유통학회) 1997 한국식품저장유통학회지 Vol.4 No.1

메조포러스 물질의 세공크기 조절

박천관,김종호,서곤 全南大學校 觸媒硏究所 1998 觸媒硏究 論文集 Vol.20 No.-

The pore sizes of MCM-41 and KIT-1 mesoporous materials could be controlled by the addition of 1,3,5-trimethylbenzene as a complementary chemical or the use of templates with different chain lengths such as ?? and ??. The synthesized mesoporous materials are characterized from X-ray diffraction patterns and N₂adsorption isotherms. With the addition of 1,3,5,-trimethylbenzene, the pore size of synthesized, mesoporous material increased, while the pore size distribution became broad. On the other hand, the mesoporous material synthesized using a template with a larger carbon chain such as ?? showed larger pore size with a narrow distribution.

미용사들의작업관련성 근골격계 장애에 관한 연구

박수경,최영진,문덕환,전진호,이종태,손혜숙 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.3

목적 : 미용사의 작업관련 근골격계 장애 실태와 관련 요인을 알아보고자 한 것이다. 방법 : 최종 대상이 된 미용사 267명에 대하여 2000년 3월부터 5월까지 Karasek의 J7Q와 N10SH의 표준화 설문지를 이용하여 일반적 특성, 직무관련 스트레스, 근골격계 장애 자각증상 등을 자기 기입식으로 작성하게 하였다. 결과 : N10SH 감시기준에 의한 근골격계 자각 증상 호소율은 전체적으로 94.4%으로 높은 편이었으며, 각 신체부위별로는 어깨 부위(61.0%), 목 부위(59.9%), 허리 부위(53.2%), 손 및 손목 부위(41.6%)의 증상 호소율이 비교적 높게 나타났으며, 상대적으로 무릎 및 허벅다리 부위(36.7%), 발 및 발목 부위(34.8%), 등 부위(28.8%), 팔 및 팔꿈치부위 (28.5%), 종아리 부위 (28.5%), 손가락 (22.8%) 등은 낮았다. 다변량 분석 결과, 미용사들의 WRMDS는 직무 스트레스와 건강관련 습관 등에 영향을 받은 것으로 관찰되었으며, 부위별로 목부위는 직무의 불안정성, 허리부위는 여자, 음주, 결정의 허용범위, 손 및 손목부위는 음주, 무릎 및 허벅다리 부위는 흡연, 발 및 발목부위는 흡연, 결정의 허용범위, 그리고 등부위는 직무의 불안정성 등이 유의한 요인으로 관찰되었다. 결론 : 주관적인 증상 호소만을 이용한 제한점에도 불구하고 산업보건의 명확한 대상이 되지 않고 있는 미용사 등의 서비스업 종사자에 대한 WRMDS 연구의 기초자료로서 활용될 수 있을 것이다. Objectives : To determine the prevalence and related factors of work related musculoskeletal symptoms in hairdressers. Methods : Informations on general characteristics, job strain, and musculoskeletal symptoms were obtained by a self-administered questionnaire, through adopting NIOSH instrument and JCQ (Job content questionnaire) , from 267 hairdressers, between March and May, 2000. Results : The symptom prevalence by N10SH surveillance criteria in total was high as 94.4%; shoulder (61.0%), neck (59.9%), low back (53.2%), hand and wrist (41.6%), etc. In multiple logistic regression, significant factors affecting the symptoms by body region , job insecurity on neck; gender, alcohol, decision latitude on low back; alcohol on hand and wrist; smoking on thigh and knee; smoking, decision latitude on ankle and foot; job insecurity on upper back. Conclusions : Musculoskeletal symptoms of hairdresser were highly prevalent, and associated with job strain and their health habits. This Is a basic data of work related musculoskeletal disorders among hairdressers who are not the subjects of occupational health service at the present time in Korea.

우리나라 화학물질 분류 및 표시체계 분석

박종안,장봉기,이종화,김은화,김춘득,여은영,남상훈 순천향대학교 기초과학연구소 2004 순천향자연과학연구 논문집 Vol.10 No.2

Classification and Labelling system of Chemicals is a very important element for management of chemical safety. Classification and labelling system of Chemicals is very different among countries. This becomes a barrier for transportation of chemicals across counties. Recently, several international organizations (OECD,UNEP,ILO etc.) endeavor to harmonize classification and labelling of chemicals. In this study, we analyze classification and labelling system of chemical described in several regulations of our country, and examine acivities of IOMC(Inter-Organization Programme for the Sound Management of Chemicals)for harmonization of classification and labelling of chemicals. The Toxic Chemical Control Law, the Fire Services Law and the Industrial Safety and Health Law are the major regulation of management of chemical safety. Classification and labelling systems of chemical vary among several regulations. Particularly, labelling system of chemicals in transport vehicles are not appropriate in response to chemical accident. We suggest that task force team consisted with inter-administrative branches is needed to harmonize classification and labelling of chemicals, and recommend the introduction of UN number in labelling systems of chemicals in transport vehicles.

종교에 대한 인간주의적 접근과 종교학적 환원 : Daniel L. Pals의 논의를 중심으로

박종천 서울대학교 종교문제연구소 2003 종교와 문화 Vol.0 No.9

α- 메틸스티렌 이량체의 촉매분해반응

박천관,김종호,서곤 전남대학교 촉매연구소 2000 觸媒硏究 論文集 Vol.21 No.-

Decomposition of α -methylstyrene dimer was studied over acid and base catalysts relating to their acidity and basicity. High conversion was obtained over KIT-1 mesoporous material with a large amount of hydroxyl group compared to strong acid and base catalysts. Dehydration and acid-base treatments of KIT-1 catalysts showed the strong contribution of hydroxyl group to the decomposition reaction. The role and characteristics of hydroxyl group as an active site for the decomposition of α-methylstyrene dimer was discussed.