인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구

이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

주성분 분석 기법을 이용한 심음 인식

이상민,홍승홍,Lee, Sang-Min,Hong, Seung-Hong 대한전자공학회 2001 電子工學會論文誌-SC (System and control) Vol.38 No.5

Recently many researches concerning heart sound analysis are being processed with development of digital signal processing and electronic components. But there are few researches about recognition of heart sound, especially full cardiac cycled heart sound, In this paper, a new recognition methods about. full cardiac cycled heart sound was proposed. For the first, the database was built by principal components analysis on training heart sound set. This database is used to recognize new input of heart sound, Ilear sounds were classified into seven classes such as normal(NO) class, pre-systolic murmurr(PS) class, early systolic murmur(ES) class, late systolic murmurr(LS) class, early diastolic murmur(EI) class, late diastolic murmur(LD) class and continuous murmuru(CM) class. As a result, we could verify that our new method has better efficiencies for the recognition the characteristics of heart sound than any precedent research. The maximum recognition rates of the new method are 71% for NO, 80% for PS and ES, 78% for LS, 87% for ED, 60% for LD and 20% for CM. Although the present results aren't practically sufficient to use our new method in recognizing heart sound, the importance of this paper is for recognition of heart sound within full cardiac cycle. We can get a better result by building a more efficient database. 최근에 디지털 선호처리와 전자부품의 발달로 심음 분식에 관한 많은 연구가 진행되고 있다. 그러나 심음 인식, 특히 심음 한주기 전체에 대한 인식연구는 거의 없다. 본 논문에서 심음 전체 한주기에 대한 새로운 인식 방법을 제안하였다. 먼저 주성분 분석을 이용하여 훈련 셋트로 데이터베이스를 구축한다. 데이터베이스는 새로운 심음입력을 인식하는데 이용된다. 심음은 정상심음, 수축전 심잡음, 수축초기 심잡음, 수축 말기 심잡음, 이완 초기 심잡음, 이완 말기 심잡음, 연속적 심잡음으로 분류된다. 실험결과 새로운 인식 방법은 심음의 특징을 인식하는데 효과적이었다. 최대 인식률은 NO의 경우 71%, PS와 ES의 경우 80%, LS의 경우 78%, ED의 경우 87%, LD의 경우 60%, CM의 경우 20% 이었다. 현재의 결과가 실제적으로 심음을 인식하기에는 충분하지 못하였지만 선음 전체 주기를 대상으로 한 연구라는데 의의가 있으며 더 효과적인 데이터베이스를 구축함으로써 인식률을 개선할 수 있다.

Valine 및 Alanine 분자로 표면 처리된 수용성의 ZnS 나노입자의 합성 및 특성연구

이상민,김기문,황청수,Lee, Sang-Min,Kim, Ki-moon,Hwang, Cheong-Soo 대한화학회 2009 대한화학회지 Vol.53 No.5

본 논문에서는 구조가 간단하고 생체분야에 응용 가능한 valine, alanine등의 아미노산 분자를 이용하여 수용성 ZnS 나노입자를 합성하였다. 얻어진 고체 생성물들은 XRD, HR-TEM, 및 EDXS분석으로 그 특성을 조사하였다. HR-TEM image 분석의 결과 크기가 대략 3.3에서 3.6 nm 의 입자들이 만들어 졌음을 확인 할 수 있었으며, 나노 입자 표면의 아미노산 분자들은 FT-IR 과 FT-Raman spectrum 을 얻어 결합 모드 등을 분석하였다. Water soluble ZnS nanocrystals were synthesized by capping the surface of the nanocrystals with valine and alanine molecules, which are structurally simple and bio friendly amino acids. The obtained ZnS nanocrystal powders were characterized by XRD, HR-TEM, and EDXS spectroscopies. The measured particle sizes by HR-TEM images were in the range of 3.3 to 3.6 nm. In addition, the surface capping amino acids molecules were characterized by FT-IR and FT-Raman spectroscopies.

지역특성을 고려한 학교규모 적정화 방향 설정에 관한 연구

이상민,조창희,Lee, Sang-Min,Cho, Chang-Hee 한국교육녹색환경연구원 2016 교육·녹색환경연구 Vol.15 No.1

본 연구는 적정규모학교의 효율적인 육성을 위하여, 적정규모학교에 대한 이론적 고찰, 국내 학생수 및 학교현황 분석, 적정규모학교 육성사업 현황분석, 적정규모학교 가이드라인(안) 제안 등의 순서로 연구를 진행하였다. 적정규모학교 기준(안)은 시 도별 특성과 지역의 여건을 고려하며, 기존 선행연구들의 교육적 효과와 교육과정의 운영 효율성을 고려하여 이상적인 방향으로 제안하고자 한다. 본 연구에서는 적정규모학교의 효율적인 육성을 위하여, 적정규모학교에 대한 이론적 고찰, 국내 학생수 및 학교현황 분석, 적정규모학교 육성사업 현황분석을 통해 적정규모학교 가이드라인(안)을 제안하였다. School appropriate scale refers to the size of the school to minimize students' educational deficits and ensure an appropriate level of students, the number of classes, class size, etc., and maximize educational effects. Difficult set of absolute standards appropriate scale school, contemporary social situations and educational effects, and seek a comprehensive and reasonable direction, considering this is required. School appropriate scale it is preferable to consider the effect of conditions in areas such as education, curriculum, but it is common to set the range in terms of school operational effectiveness, and efficiency of education funding, school environment. So the concept of the appropriate scale including the school's status changes associated with school size, is seeking a new direction is needed so that the school environment, etc. are considered.

머신러닝 기법을 활용한 낙동강 중류 지역의 Chl-a 예측 알고리즘 비교 연구(수질인자 및 수량 중심으로)

이상민,박경덕,김일규,Lee, Sang-Min,Park, Kyeong-Deok,Kim, Il-Kyu 대한상하수도학회 2020 상하수도학회지 Vol.34 No.4

In this study, we performed algorithms to predict algae of Chlorophyll-a (Chl-a). Water quality and quantity data of the middle Nakdong River area were used. At first, the correlation analysis between Chl-a and water quality and quantity data was studied. We extracted ten factors of high importance for water quality and quantity data about the two weirs. Algorithms predicted how ten factors affected Chl-a occurrence. We performed algorithms about decision tree, random forest, elastic net, gradient boosting with Python. The root mean square error (RMSE) value was used to evaluate excellent algorithms. The gradient boosting showed 10.55 of RMSE value for the Gangjeonggoryeong (GG) site and 11.43 of RMSE value for the Dalsung (DS) site. The gradient boosting algorithm showed excellent results for GG and DS sites. Prediction value for the four algorithms was also evaluated through the Receiver operating characteristic (ROC) curve and Area under curve (AUC). As a result of the evaluation, the AUC value was 0.877 at GG site and the AUC value was 0.951 at DS site. So the algorithm's ability to interpret seemed to be excellent.

대장직장 종양에 대한 내시경 점막하 절제술의 연대순 임상 결과와 학습 곡선

이상민 ( Sang Min Lee ),김은수 ( Eun Soo Kim ),박경식 ( Kyung Sik Park ),조광범 ( Kwang Bum Cho ),김동춘 ( Dong Choon Kim ),강유진 ( Yu Jin Kang ),이유진 ( Yoo Jin Lee ),이정민 ( Jung Min Lee ),최은성 ( Eun Sung Choi ),최재혁 ( Ja 대한소화기학회 2014 대한소화기학회지 Vol.64 No.4

Background/Aims: Endoscopic submucosal dissection (ESD) is an effective procedure for en-bloc curative resection of the colorectal tumor. As it requires high technical skills and experience in therapeutic endoscopy, it is important to understand learning curve of ESD technique. The aim of this study was to retrospectively describe the clinical results of ESD and to evaluate learning curve for the ESD of colorectal tumors. Methods: A total of 90 patients with 90 colorectal neoplasms, who had undergone ESD at a tertiary referral hospital from July 2009 to December 2012, were enrolled. The ESD was performed by a single endoscopist. All ESD cases were divided into three periods: first, cases 1-30; second, cases 31-60; and third, cases 61-90. Results: The en-bloc resection rates in third period (100%) was significantly higher than that of the first (93.3%) and second period (80%) (p=0.025). The perforation rate in third period (0%) also significantly decreased compared with that of the first (13.3%) and second period (20%) (p=0.032). To calibrate the difference of tumor size among periods, proficiency was calculated, as the procedure time per specimen area (min/cm2). The proficiency in third period (4.3) was significantly shorter than that of the first (16.8) and second period (10.2) (p=0.004). Conclusions: The learning curve of colorectal ESD in our study shows that at least 60 cases of ESD have to be conducted to acquire sufficient skill of degree without perforation. (Korean J Gastroenterol 2014;64:198-205)

교육에서의 소진에 관한 이론적 고찰

이상민,안성희,Lee, Sang Min,An, Sunghee 연세대학교 의과대학 2014 의학교육논단 Vol.16 No.2

Burnout research has mostly been performed in human service fields. However, it has recently been expanded to cover diverse settings and even non-occupational samples, such as students. In this study, we defined the construct of academic burnout, which is the burnout experienced by students. Next, we described the concept of academic burnout by introducing measurements for assessing academic burnout. On the basis of the demand-control model (DCM) and effort-reward imbalance model (ERIM), which are the most predominant theoretical burnout models, we described the causal factors and the pathway to experiencing academic burnout symptoms. The ERIM was a more influential model than the DCM when explaining the academic burnout of Korean students. Based on the results of previous longitudinal studies on academic burnout, we recognized emotional exhaustion and academic inefficacy as the initial symptoms of academic burnout. Finally, we discussed the prevention and intervention programs with specific components that should be included in those programs.

선박조종시뮬레이터를 활용한 예부선의 유체력 간섭 특성에 관한 연구

이상민,조상희,Lee, Sang-Min,Jo, Sang-Hui 해양환경안전학회 2009 海洋環境安全學會誌 Vol.15 No.1

예부선 운항 시 부두의 접안, 이안 및 좁은 수로를 항행할 경우 접할 수 있는 안벽 근처의 항행과, 두 선박이 접근하여 평행 항로상을 반대 방향으로 항과할 경우의 유체력 상호작용을 살펴보기 위하여, 예선의 부선 예항 시뮬레이션을 실시한 후 부선의 선수방향, 예선의 회두 모멘트와 횡방향의 힘과 같은 특성을 조사 및 분석하여 그에 따른 안전한 예부선의 조선 방안을 제시하였다. 그 결과 부선의 과도한 회두운동을 감소시키기 위해서는 예선의 속도가 너무 느리지 않도록 미속상태의 속도를 유지하고, 예인삭의 길이를 가능한 한 부선의 길이만큼 줄여 항행하는 것이 안전예항 업무에 도움이 될 것으로 판단된다. In order to investigate the hydrodynamic interaction between the tug-barge and bank or ship which is crossing to the opposite direction, the towing simulations of tug-barge transportation were performed. Heading of barge, yaw moment and lateral force of tug boat were obtained by this simulation. The characteristics of results were analyzed and the safety towing method for tug-barge operation was proposed. In order to reduce the slewing motion of barge for safe towing operation, the speed of tug boat should be kept slow ahead state with shortened towing line as length of barge within the limits of the possible.

휴대용 GPS를 이용한 부선의 안전예항시스템 구축에 관한 연구

이상민,안병길,Lee, Sang-Min,Ahn, Byung-Kil 해양환경안전학회 2008 海洋環境安全學會誌 Vol.14 No.4

피예선인 부선으로 인하여 예선 자체의 조종성능에 제한을 받고, 이러한 예부선은 바람, 파도, 조류 등과 같은 외력의 영향이 크게 작용하여 예부선 운항의 안정성 확보에 많은 어려움이 수반되고 있다. 예선의 선장, VTS 및 육상의 운항부서 등에서는 부선과 같은 피예선의 실해역에서의 침로, 속도, 위치 등을 실시간으로 추적하여 해난사고를 사전에 예방할 수 있는 시스템의 구축이 절실히 필요한 실정 이다. 본 연구에서는 비교적 저렴하고 이동이 용이한 휴대용 GPS를 이용한 부선의 안전예항시스템의 구축에 대한 기초적인 방법을 제안하고, 이를 토대로 실선에서의 테스트를 실시하고 그 결과에 대하여 논의한다. 본 연구를 통하여 안전 예항에 필요한 여러 항해 정보를 취득할 수 있었으며, 이러한 정보는 다양한 분야에서의 활용이 가능할 것으로 사료된다. The tug boat is restricted in her maneuvering ability due to the towed barge, and tug-barge has been strongly affected by the external forces, i.e. the wind, wave, currents, and so on. Therefore, it is difficult to get the safety of tug-barge operation. It is necessary for the captain of tug boat, VTS and operation department to develope the system which can keep observing the course, speed and position of the towed barge in actual sea and prevent the marine accidents in advance. In this study, we proposed the basic method to develope the safety towing system for barge using portable GPS which was easily movable and relatively inexpensive. Then we conducted the test on the real barge. As a result, we could obtain diverse navigation data to make a safety towing work. It was noted that these data could be used for carious purposes.

대식세포주에서 인슐린이 $I{\kappa}B/NF-{\kappa}B$ 경로 활성화에 미치는 영향

이상민,장연실,이춘택,김영환,한성구,심영수,유철규,Lee, Sang-Min,Jang, Yeon-Sil,Lee, Choon-Taek,Kim, Young-Whan,Han, Sung-Koo,Shim, Young-Soo,Yoo, Chul-Gyu 대한결핵및호흡기학회 2010 Tuberculosis and Respiratory Diseases Vol.68 No.3

Background: Sepsis still has a high mortality rate despite adequate supportive care. Newer therapeutic modalities have been developed but they have generally ended in failure. Recently, insulin was reported to have an anti-inflammatory effect by inhibiting the $I{\kappa}B/NF-{\kappa}B$ pathway, and may have therapeutic potential in sepsis. However, the precise mechanism of the anti-inflammatory effect of insulin is unclear. This study examined the role of insulin in activating $I{\kappa}B/NF-{\kappa}B$ in macrophage. Methods: Raw 264.7 cells, a murine macrophage cell line, were used in this experiment. Western blotting using $I{\kappa}B$ Ab and phosphor-specific $I{\kappa}B$ Ab was performed to evaluate the degradation and phosphorylation of $I{\kappa}B$ cells. For the $I{\kappa}B$ Kinase (IKK) activity, an immune complex kinase assay was performed. The level of interleukin-6 (IL-6) was measured by ELISA to determine the level of proinflammatory cytokine. Results: $I{\kappa}B{\alpha}$ degradation began 30 min after lipopolysaccharide (LPS) treatment. However, an insulin pretreatment suppressed the $I{\kappa}B{\alpha}$ degradation caused by the LPS treatment. The phosphorylation of $I{\kappa}B{\alpha}$ and IKK activity was also inhibited by the insulin pretreatment. Finally, the insulin pretreatment showed a tendency to suppress the induction of IL-6 by LPS. Conclusion: Insulin might have an anti-inflammatory effect though partial inhibition of the $I{\kappa}B/NF{\kappa}B$ pathway in macrophage cell lines.