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부산지역 설사환자에서 분리한 Campylobacter jejuni의 항균제 감수성과 유전자형 분석
박선희,김병준,권영희,황인영,성경혜,박은희,진성현 대한임상미생물학회 2019 Annals of clinical microbiology Vol.22 No.2
부산지역 설사환자에서 분리한 Campylobacter jejuni 의항균제 감수성과 유전자형 분석부산광역시 보건환경연구원 감염병연구부 미생물팀박선희, 김병준, 권영희, 황인영, 성경혜, 박은희, 진성현배경: Campylobacter는 식품매개질환의 중요한 원인균으로, 본 연구에서는 부산지역 설사환자에서 분리한 Campylobacterjejuni의 분리경향, 항균제 내성률, 유전자형 분석에 대해 조사하였다. 방법: 2014년부터 2017년 9월까지 급성설사질환 감시사업 및 5건의 식중독 사례에서 97주의 Campylobacter jejuni를 분리하였다. 항균제 감수성 검사는 9종류의 항균제(ciprofloxacin (CIP), nalidixic acid (NAL), tetracycline (TET), chloramphenicol,azithromycin (AZI), erythromycin (ERY), streptomycin (STR), gentamicin, telithromycin)에 대하여 액체배지미량희석법으로 실시하였고, 유전자형은 pulsed-field gel electrophoresis (PFGE)로 분석하였다. 결과: Campylobacter jejuni의 4년간 평균 분리율은 2.0%였으며 해마다 분리율은 증가하였다. 항균제 내성률은 NAL90.9%, CIP 89.4%, TET 13.6%, AZI와 ERY 각 3.0%, STR 1.5% 순이었고, 다제내성률은 18.2%였으며 다제내성균주 모두quinolone계 항균제(CIP-NAL)를 포함하고 있었다. SmaI 제한효소로 처리한 PFGE 패턴은 모두 17개 cluster로 분류되었으며 이 중 cluster 11이 부산지역에서 분리되는 Campylobacter의 주요 유전자형 패턴이었다. 결론: 본 연구는 적절한 항균제 사용과 항균제 내성 관리를 위한 유용한 자료를 제시하며, 향후 증가가 예상되는Campylobacter로 인한 식중독의 역학 조사를 위한 자료 축적에 도움이 될 것이다.
박선희,김주연 한국미용학회 2020 한국미용학회지 Vol.26 No.2
Purpose of this study is to evaluate the usefulness of fermented bamboo shoot sheath (BSS) for functional cosmetics. BSS was fermented using the mixed culture of Lactobacillus plantarum and Weissella cibaria. Antioxidant compounds, antioxidative activity, beauty-related enzymes (tyrosinase, collagenase, and elastase) inhibition, antibacterial activity, and keratinocyte growth activation effect of unfermented and fermented BSS extract were analyzed in the same way. The content of anthocyanin, polyphenol, total phenolic compounds, and total flavonoid in unfermented BSS extract were 1.08 mg/g, 43.67 mg/g, 17.14mg/g, 1.13mg/g, respectively and those in fermented BSS extract were increased to be 1.33mg/g, 52.95mg/g, 23.89mg/g, 1.62mg/g, respectively. ABTS radical-, DPPH radical-, and nitrite radical– scavenging activities of unfermented BSS extract were 55%, 62%, and 49%, respectively, and those of fermented BSS extract were 64%, 73%, and 53%, respectively. The tyrosinase- and collagenase-inhibitory activities of both unfermented and fermented BSS extract were approximately 80% and 25%, respectively. Meanwhile, the elastase-inhibitory activity of unfermented BSS extract was not detected but that of fermented BSS extract was shown to be 10%. The antibacterial activity of unfermented BSS extract was not detected but fermented BSS extract inhibited growth of Gram(+) Staphylococcus epidermis and Gram(-) Escherichia coli. Growth of keratinocyte cell line (HaCaT) was promoted by unfermented and fermented BSS at the level of 10 and 25%, respectively. Thus, the water extract of BSS was useful as the ingredient for functional cosmetics and the usefulness of specific factors was improved by the fermentation.
박선희,조선영,최수한,최지연,손희정,김홍빈,이미숙 대한의료관련감염관리학회 2022 의료관련감염관리 Vol.27 No.2
The Korean National Healthcare-associated Infections Surveillance System (KONIS) started surveilling healthcare-associated infections (HAIs) in intensive care units in 2006. Since then, the KONIS modules have expanded, and the participating hospitals have diversified. To allow for these changes, surveillance indicators need to be improved to represent national data and provide useful benchmarks. Herein, we reviewed the national HAI surveillance systems in 11 countries and the European Union, which were searched online during October–December 2019, and compared the target healthcare facilities, indicators, and surveillance methods. Twelve experts independently evaluated the priorities in terms of disease burden, relevance, intervenability, urgency, applicability, acceptability, barriers and facilitators of implementation on a scale of 0-10 in each category, and the highest score had the highest priority. This review identified five areas of improvement. First, new surveillance nfl indicators that require web-based automated systems can be introduced. These would include laboratory-based surveillance, such as Clostridioides difficile infection and multi-drug resistant organisms, and surveillance of antimicrobial resistance and use. Second, surveillance areas can be expanded to general or specialized wards, according to the needs of the participating hospitals. Third, healthcare facilities, such as outpatient dialysis clinics or outpatient surgical centers, can be included in the KONIS. Fourth, standardized infection ratios (SIRs) and standardized utilization ratios (SURs) can be introduced as effective benchmarks. Finally, the point prevalence survey can play a supplementary role in identifying new HAIs and help allocate efforts to their prevention. Among these points, the use of SIR and SUR was considered a top priority indicator for the KONIS. As the KONIS continues to evolve, it is necessary to introduce new indicators and benchmark methods to address these changes. It is of utmost importance that the KONIS be operated stably and steadily, and new enrollees in the KONIS need to understand and adapt to the KONIS before introducing new indicators. In addition, it should be evaluated which indicators and benchmarks can be well-incorporated and appropriately used in the KONIS and the government should make efforts to establish an automated surveillance system using electronic medical information.