한의진단명과 진단요건의 표준화 연구 II (표준화 실례) : 2차년도 연구결과 중간 보고

양기상,최선미,최승훈,안규석,박경모,박종현,김성우,신승호,정우열,전병훈,고현,김정범,신상우,김성훈,김동희,권영규,엄현섭,장혜옥 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the second year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows; - differential diagnosis according to condition of body fluid, differentiation of syndromes according to the state of qi and blood, differential diagnosis according to relative excessiveness or deficiency of yin and yang(氣血陰陽津液辨證) - differentiation of diseases according to pathological changes of the viscera and their interrelation - analyzing and differentiating of febrile diseases in accordance with the theory of the six channels(傷寒辨證) The individual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name(異名), notion of diagnosis pattern, index of differentiation of symptoms and signs(辨證指標), the main point of diagnosis, analysis of diagnosis pattern(證候分析), discrimination of diagnosis pattern(證候鑑別), a way of curing a diseases(治法), prescription(處方), herb in common use(常用藥物), diseases appearing the diagnosis pattern(常見疾病), documents(文獻調査). This study was carried out on the basis of the Chinese documents and references.

당뇨병 백서의 간세포에서 Glucokinase 활성도 및 유전자 발현에 대한 인슐린의 영향

강성이,팽정령,서광식,안규정,우정택,김성운,양인명,김진우,김영설,김광원,최영길 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-

목적 당대사의 조절 상태에 따른 생체 변화를 분자 수준에서 이해하고자 식이 조건을 달리한 정상 백서와 화학적으로 유도된 당뇨병 백서의 간조직에서 혈당수준과 인슐린치료 정도에 따라 나타나는 글루코키나제 활성도 및 유전자 발현을 분석하였다. 방법 스트렙토조토신 정맥투여 후 당뇨병의 유발을 확인하고, 인슐린을 1일 3회 3일간 복강내로 투여하여 상태를 안정시킨 후, 인슐린 투여군은 인슐린 투여 6시간이내에 그리고 인슐린 비투여군은 24시간 후 단두하여 채혈하고 복강을 열어 간조직을 채취하였다. 채취한 간조직에서 글루코키나제 활성도는 인산화된 포도당에서 NADH의 형성을 형광분광계로 측정하였으며, 글루코키나제 유전자 mRNA발현은 Northern 분석법을 이용하였다. 성적 정상 백서에서 공복상태와 식이를 섭취한 경우에 간조직의 글루코키나제 효소의 활성은 차이가 없었으나, 글루코키나제 유전자 mRNA 발현은 증가되었다. 당뇨병이 유발된 백서의 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현은 정상 백서에 비하여 낮았다. 인슐린 투쳐 후 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현이 증가되었고, 특히 혈당이 정상화된 경우에서 글루코키나제 유전자의 mRNA 발현이 증가도었다. 결론 인슐린에 의한 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA을 증가를 볼수 있었다. 당뇨병 백서에서 인슐린 투여 후에 혈당조절이 안된 경우 간조직의 글루코키나제 유전자의 mRNA 발현이 증가가 없는 것으로 보아 글루코키나제 mRNA의 발현에는 인슐린 이외의 다른 요소가 관여할 것으로 생각된다. The liver-specific hexokinase isoenzyme, referred to as glucokinase, is thought to play a key reglulatory role in hepatic glucose metabolism. The glucokinase gene is, therefore, of interest both because of its tissue-specific expression and because of the several regulatory processes that can be analyzed. The level of hepatic glucokinase activity appears to be determined essentially by regulation of the rate of enzyme synthesis, with insulin playing a leading role as an inducer. We investigated the role of insulin for the induction of glucokinase in the liver of diabetic rats. Experimental diabetes was induced by injection of streptozotocin 7 days before the experiment. Regular insulin was given by three days intraperitoneal injection at 8-h interval. The glucokinase mRNA in the liver was estimated by Nothern blot assay, as well as by fluorometric enzyme activity assay. Glucokinase activity was not reduced in the liver of normal fasting rats as compared to normal fed rats. And glucokinase activity was reduced in the liver of diabetic rats as compared to normal rats. In diabetic rats treated with insulin, glucokinase enzyme activity were increased. But glucokinase mRNA expression was only increased in normoglycemic diabetic rat with treated with insulin as compared to hyperglycemic rat. These data indicate that insulin stimulates hepatic glucokinase enzyme activity and mRNA expression. But other hormonal or metabolic factors may be contribute to regulation of glucokinase mRNA expression.

顔貌變形症 患者에 있어서 顎矯正 手術前 心理 評價에 關한 臨床的 硏究

양명철,신효근,진우정 大韓顎顔面成形再建外科學會 1995 Maxillofacial Plastic Reconstructive Surgery Vol.17 No.1

The need to assess preoperative psychological characteristics of orthoganathic surgery patients has recently been recognized. Fifty six patients were involved in this study, they were investigated in the preoperative psychosocial aspects. Korean Version Eysenk Personality Questionaire and Korean Version Tennessee Self-Concept Scale were used for psychological test. The results were as follows : 1. There was no significant difference of sex and age in the personality diamension, the selfconcept scale, and the preoperative tests(P>0.05). 2. 37% of subjects showed phonetic problem, 60% masticatory problem and 56% social problem for facial deformity. The first choice was the face in the order of correctionfor mastication, phonation and face. And 83% of the subjects forecasted the change in their face after surgical correction, The answer was generally vague. 64% of the subjects expected their personality to become more lively as a result of their operation. 3. Total positive self score and personal self score were less improved in the groups with high scores of toughmindness and neuroticism(P<0.05), degree of suffering was increased in the group with high scores of neuroticism(P<0.05). 4. Degree of subjective deformity, self-consciousness, and degree of dissimilarity were more improved in the group of intraversion, there was a significant correlation with degree of dissimilarity in the group with high score of toughmindness(r=-.64, P<0.05). 5. There was a significant correlation with the forecast of change in the groups with high score in extraversion(r=.59, P<0.01) and with low score in neuroticism(r=-.39, P<0.05).

NiTi-형상기억합금 차동식 액츄에이터의 동특성연구

정상화,김현욱,장우양,김경석,차경래,나윤철 朝鮮大學校 機械技術硏究所 2001 機械技術硏究 Vol.4 No.2

최근 전 세계적으로 초소형 정밀 기계 기술에 관한 연구 및 개발이 활발하게 이루어지면서 형상기억합금(Shape Memory Alloy : SMA)을 이용한 초소형 액츄에이터(Micro Actuator)에 관한 관심이 증대되고 있다. 형상기억합금을 액츄에이터에 응용하기 위해서는 동적 특성의 분석이 필수적이지만 지금까지의 연구에서는 형상기억합금의 물질 및 재료적 특성은 많이 보고되고 있으나 동적 특성에 대한 연구는 매우 부족한 실정이다. 본 논문에서는 비저항(resistivity)이 큰 NiTi합금 와이어(wire)를 이용하여 나선형 스프링(helical spring)을 제작하여 디지털 힘 측정기(Digital force gauge)와 적외선 온도센서(Infrared themometer) 그리고 레이저 변위 센서(Laser displacement sensor)를 이용하여 이 NiTi 스프링의 힘의 크기와 반응속도, 온도와 변위를 측정하여 형상기억합금의 동적 특성을 분석하였다. 또한 형상기억합금을 이용하여 차동식 액츄에이터를 제작하여 그 동적 성능을 실험하였다. In the recent years, as the research and the development of micro and precision machinery become active, the interest of micro actuators using SMA(Shape Memory Alloy) has been increased. The dynamic characteristic analysis of SMA is necessary for actuator application and many common researches report the material characteristics of SMA sufficiently. However, the research on dynamic characteristics is very deficient. In this paper, the helical spring are fabricated with NiTi SMA wire of high resistivity. The force, response speed, temperature, and displacement are measured by digital force gauge, infrared thermometer, and laser displacement sensor so that the dynamic characteristics of this SMA is analyzed. Also, bidirectional actuator was fabricated and experimented for its performance.

고감도 CaSO_4 : Dy TL 분말의 TL 특성 및 고감도 TL 소자 제작 Dy TL powder and development of high sensitivity CaSO_4 Dy TL pellet

양정선,박재우,김두영,김장렬,장시영 제주대학교 방사능이용연구소 2002 연구보고 Vol.16 No.-

CaSO_4 : Dy series TL material is widely used as a personal dosimeter because of its high sensitivity to radiation. KAERI(Korea Atomic Energy Research Institute) has been produced CaSO_4 TL powder activated by Dy(rare earth elements) for several years and continually tried to improve its sensitivity having more than that of commercialized Teledyne CaSO_4 : Dy TL powders(USA). The TL powder is prepared by adding 0.1mol% Dy as an activator and by the acid evaporation method at first. And new step of washing this powder by distil-water is appended to separate non-crystalized powder in the initial crystal of CaSO_4 : Dy powder. CaSO_4 : Dy crystal TL powder which is produced by applying this new method has higer sensitivity than before washing step, and it has also higher sensitivity than that of Teledyne CaSO_4 : Dy TL powder. This CaSO_4 : Dy TL powder shows good TL characteristics such as dose response, energy response and fading. It is not possible to make solid detectors of only CaSO_4 : Dy TL phosphor without any binding material. Until now widely used sintered pellets. which were made from a mixture of CaSO_4 : Dy TL phosphor and Teflon. But, due to reduction in content of CaSO_4 : Dy TL phosphor in pellets(15∼20wt%) the TL sensitivity of pellets was reduced correspondingly. To variously use the CaSO_4 : Dy TL phosphor, research for preparing detectors by mixing CaSO_4 : Dy TL phosphor with different binding substances has been conductedThis paper described development of a new type of CaSO_4 : Dy pellets by using P-compounds as a bonding material(KCT-300), and compared the TL sensitivity with that of the commercialized Teledyne CaSO_4 : Dy pellets. Sensitivity of a new developed KCT-300 shows about 6 times than Teledyne ones, and can be used to measure very low radiation dose.

리튬이 도핑된 막을 이용한 실리콘-실리콘 접합

정지원,주병권,최우범,정성재,이남양,최두진,오명환 경북대학교 센서기술연구소 1997 센서技術學術大會論文集 Vol.8 No.1

Silicon-to-silicon anodic bonding was accomplished using lithium doped interlayer. Lithium doped interlayers were deposited on silicon substrates by electron beam evaporation, which has a fast deposition rate compared with sputtering method. Silicon-to-silicon bonding occurs in the range of temperatures from 250℃ to 300℃ with the applied voltages from 70V_(DC) to 100V_(DC). The bonding strength obtained from tensile test was about 5MPa under the condition of 80V_(DC) in 300℃. The surface morphology of lithium doped interlayer was studied with the atomic force microscopy (AFM). Finally, secondary ion mass spectroscopy (SIMS) analysis was carried out in order to investigate the role of the lithium ions in bonding mechanism.

대장암종에서 암관련 유전자들의 Promoter Methylation과 암종 발생 및 진행과의 관게에 대한 연구

정동준,이정은,민영기,조성우,백무준,양승하,김의한,김창진 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

The colorectal corcinoma is one of the most common malignant tumor in Korea and its carcinogenic mechanism has not been elucidated exactly yet. So far, the most common carcinogenic mechanism has been known to be multiple genetic changes. But, the CpG island hypermethylation has been revealed to be one of the most important carcinogenic mechanism second to the genetic changes. The epigenetic changes characteristically imprint and transfer to the next generation without changes of nucleotide sequences. When the CpG island of promoter or 5'- exon 1 of a specific gene is methylated by DNA methyltrasferases (DNMTs), the gene expression is inhibited. Carcinogenic mechansisms by promoter methylation has been well known, but the progression of the cancer has not been elucidated yet. The molecular researches on the carcinogenic mechanisms largely depended on the tumor tissue containing various components of cell, due to difficulties in harvesting pure homogeneous tumor cells. Promoter methylatonof the cancer related genes as APc, hMLH2, p16, DAP-K and MGMT in 50 cases of sporadic colorectal carcinoma was studied. The pure normal colorectal epithelial cells, and cancer cells from superficial and deep invasive portion were harvested respectively by laser captured microdissection(LCM), and DNA was extracted from them. The promoter methylation was studied by methylation specific poymerase reaction (MSP) after bisulfite modification of the DNA and the expression of the genes by immunohistochemistry. The results suggests that promoter methylation of the cancer related genes are closely related with the colorectal carcinogenesis and it occurrs from early phase of carcinogenesis, progressing constantly to the late stage. And the promoter methylation of normal epithelial cells increased by age. The p15, known to be the genes related to hematologic malignancy, was closely related to the colorectal carcinoma as well.

인슐린 주사제의 고충실 액체색층기 분석

우정택,팽정렬,김성운,양인명,김진우,김영설,김광원,최영길 최신의학사 1993 最新醫學 Vol.36 No.8

방사선 조사를 받은 흰쥐 망막 속얼기층의 미세구조

양석우,고정식,양남길,안의태,박경호 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.2

본 실험은 머리부분이 많은양의 방사선에 노출되었을 때 망막 속얼기층의 미세구조적 변화를 관찰하기 위하여 시행하였다. 실험동물로는 체중 200-250g의 흰쥐를 사용하였으며, 마취상태에서 머리부분에만 방사선을 조사하였다. 방사선 조사는 미쓰비시 선형가속기를 이용하여 조사거리 80cm, 구역 30cm X 30cm, 깊이 1.2cm 조건에서 1분당 200rad씩 연속조사하여, 3,000rad 또는 6,000rad를 조사한 후 6시간, 2일, 6일후에 희생시켰다. 각 동물은 1% glutaraldehyde- 1% paraformaldehyde액으로 관류고정하였다. 관류가 끝난 후 안구를 적출하여 톱니둘레를 따라 절단한 다음, 망막의 일정부위에서 조직을 떼어냈다. 떼어낸 조직은 2.5% glutaraldehyde- 1.5% paraformaldehyde액에 일차고정한 후 1%osmium tetroxide액에 이차고정하였다. 고정된 조직은 수세와 탈수과정을 거쳐 araldite혼합액에 포매한 다음 얇은 절편을 작성하여 uranly acetate와 lead citrate로 염색한 후 전자현미경으로 관찰하여 다음과 같은 결과를 얻었다. 1. 무축삭신경원의 세포질돌기는 전자밀도가 높은 어두운세포와 전자밀도가 낮은 밝은세포로 구분되었으며, 밝은 세포 가운데에는 과립형연접소포를 갖고 있는 돌기가 관찰되었다. 2. 3,000rad조사 후 6시간군의 경우 밝은 무축삭신경원이 세포질돌기와 신경절세포의 가지돌기는 팽대되었으며 사립체와 세포막이 파괴되는 모습이 관찰되었는데, 2일군의 경우 그 경향이 가장 심했다. 3. 3,000rad조사 후 6일군의 경우 부챗살아교세포의 영역이 넓어져서 전체적인 조직의 모습이 2일군의 것에 비하여 많이 회복되어 보였다. 4. 6,000rad조사 6일군의 경우 부챗살아교세포는 미세사가 많고 전자밀도가 높은 변성된 세포가 자주 관찰되었다. 이상의 결과를 종합해 보면 3,000rad의 방사선을 조사받으면 흰쥐망막의 속얼기층은 처음에는 무축삭신경원의 세포질돌기와 신경절세포의 가지돌기가 손상을 받았으나, 6일 후에는 부챗살아교세포의 영역이 넓어져서 많이 회복되었다. 그러나 6,000rad정도의 방사선을 조사하면 부챗살아교세포도 변성되어 속얼기층 전체가 변성된것으로 보아, 부챗살아교세포는 무축삭신경원이나 신경절세포에 비하여 방사선에 대한 저항성이 높다고 생각된다. This experiment was performed to study the morphological respones of the inner plexiform layer of the retina of rat following X-ray irradiation. Rats anethetized with sodium thiopental, were exposed only on their head areas with a single dose of 3,000rads or 6,000rads, respectively. Radiation was produced by Mitsubishi linear accelerator, at the speed of 200rads/min. The experimental groups were sacrificed on the 6th hour, 2nd hour, 2nd and 6th day after X-ray irradiation. Under anethesia, 1% glutaraldehyde- 1% paprformaldehyde solution was perfused through the heart. Pieces of the tissue taken from the posterior region of the retina were fixed in 2.5% glutaraldehyde- 1.5% paraformaldehyde, and in 1% osmium tetroxide, and embedded in araldite mixture. Ultrathin sections were stained with uranyl acetate and lead citrate solutions. From the ultrastructural study, following results were made: 1. Two types of amacrine cells, according to their elecrton densities, were found, i.e., light amacrine cell and dark amacrine cell. Granular synaptic vesicels were often observed in the light amacrine cells. 2. Two days after exposure to 3,000rads of X-ray, severe morphological changes were observed in cytoplasmic processes of light amacrine cells and dendrite of ganglion cells; i.e., edematous changes, framoentation of mitochondria, breakdown of cell membranes, etc. 3. Six days after exposure to 3,000rads of X-ray, many of the malformaitons were seen to be repaired, except the territory of activated Muller cell. 4. Six days after exposure to 6,000rads of X-ray, darkly degenerated Muller cell were frequently observed. From the above results, it was concluded that large amount of head-irradiation results the severe damages to the cytoplasmic processes of amacrince cell and the dendrites of ganglion cell in the inner plexiform layer of rat retina, but Muller cells are more radioresistant than amacrine and ganglion cells.

수도권 지역의 의료기기 관련 업체의 실태 조사 연구

양태홍,이우철,김정래,박승환 서울보건대학 1997 서울보건대학 부설 병원경영연구소 논문집 Vol.3 No.1

Recently, the domestic business of medical equipment have many difficult things in the development and sale of new product. To overcome them, it is needed to solidify the co-work system of industry-education marriage. This paper is undertakened to serve an aid in the field of medical equipment. The analysis of data is attempted through the survey by questionaires, and an object of study is the medical business corporation at Seoul area. As a result to review the data it was identifided that this study is able to be applied to the basis material to reconstruct a right role and phase to medical business Also, it`s expected that we're able to use its results as the educational materials to bring up the more qualified students fit to the industrial need.