Transcriptome profiling of olive flounder responses under acute and chronic heat stress

Woo‑Jin Kim,이규빈,Dain Lee,Hyun‑Chul Kim,Bo‑Hye Nam,Hyungtaek Jung,이선주,Kyunghwan Kim 한국유전학회 2021 Genes & Genomics Vol.43 No.2

Background The olive founder (Paralichthys olivaceus) is a saltwater fsh, which is valuable to the economy. The olive founder strives to adapt to environmental stressors through physiological, biochemical, and transcriptional responses. The rise in water temperature threatens the growth, development, reproduction, and survival of olive founder. Each organ in the olive founder can diferentially respond to heat stress. Objectives The purpose of this study is to investigate organ-specifc transcriptional changes in olive founder tissues during heat stress. Methods In this study, transcriptome dynamics of the gill, liver, and muscle of olive founder to acute or chronic heat stress were investigated. Results Principal component analysis plotting revealed that the transcriptome of each organ is quite separated. K-means clustering, gene ontology, and Kyoto Encyclopedia of Genes and Genomes pathway analysis showed the diferential transcriptome responses of each organ to heat stress. Heat stress commonly afects the pathways involved in the correct protein folding, DNA repair, and cell cycle. Conclusion Our results may provide a valuable molecular basis of heat acclimation in fshes.

2003년 국내 중증급성호흡기증후군 진료 현황 및 문제점 분석

이진수,김은실,정문현,백제중,정선화,안주희,최영화,이선희,고철우,김성범,김민자,박승철,기현균,송재훈,최상호,김양수,이상오,조용균,박영훈,정숙인,김연숙,이흥범,손창희,장성희,정희진,김우주 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 2002년 말 중국에서 SARS가 발생한 이후 국내에서도 2003년 10월까지 총 3명의 추정환자, 17명의 의심환자가 보고되었다. 향후 추가적인 SARS의 유행이 우려되는 상황에서, 그간의 SARS 환자 진료에 있어서의 실질적인 준비사항, 진료 현황 등에 대한 조사를 통해 문제점을 파악하여, 향후 더 나은 대비가 될 수 있도록 개선점을 제시하고자 하였다. 재료 및 방법 : SARS로 의심되는 환자를 진료 경험이 있는 병원의료진을 대상으로 2003년 10월에 설문조사를 실시하였다. 설문에는 SARS 환자 진료 시의 실질적인 조치, 진료 현황, 병실, 응급실 및 외래에서의 격리 시설과 준비사항, 보건당국의 관리와 지원에 관한 사항을 포함하였다. 결과 : 대상이 되는 22개 병원 중 17개(17/22, 77.2%) 병원이 설문에 응하였다. SARS 환자를 위한 격리실은 응급실, 외래, 일반병실 및 중환자실에서 각각 9개(9/17, 52.9%), 5개(5/17, 29.4%), 15개(15/16, 93.7%), 4개(4/16, 25%) 병원에서 음압처리가 되어있지 않은 일인실 혹은 다인실이 사용되었고, 1개(1/16, 6.3%) 병원에서만 일반병실에서 음압격리실이 운영되었다. 입원환자의 진찰 시 개인보호구의 착용은 거의 모든 의료기관에서 이루어졌다. 보건당국에서 SARS지정병원의 시설 등을 사전에 확인한 곳은 1곳(1/12, 8.3%)이였고, 14개 병원(14/15, 93.3%)에서는 보건당국에 의뢰한 검사결과를 통하 받지 못하였다. 결론 : 의료기관에서 SARS 환자용 격리실뿐만 아니라 기존의 격리실 설비 등이 미흡하였으며, 특히 중환자실 및 외래의 준비가 더욱 부족하였다. 보건당국의 의료기관에 대한 종합적인 지원이 부족하였고, 병원과의 원활한 연계가 잘 이루어지지 않았다. SARS 만이 아닌 격리를 필요로 하는 질환의 적절한 진료를 위해 향후 병원 시설의 정비와 정부차원에서의 보다 구체적이고 실질적인 대책마련이 필요하다. Background : There was an worldwide outbreak of the Severe Acute Respiratory Syndrome (SARS) originated from China in late 2002. During that period three cases of suspected SARS and 17 cases of probable SARS were reported in Korea. With the concerns about the reemergence of SARS-coV transmission, it is important to be prepared for any possibility. So, this study is aimed to analysis the past measures in managing SARS and propose the amendatory plans to improve the preparedness. Materials & Methods : Questionnaires were collected among clinicians with any experience in managing the probable or suspected SARS cases in Oct. 2003. 17 out of 22 hospitals responded to the questionnaire. The contents in the questionnaire were practical activities, personal equipments, response plans, isolation facilities in emergency centers, outpatient clinics, general wards and intensive care units, and relationship with the public health department. Results : The dedicated isolation rooms in emergency centers, outpatient clinics, general wards, and intensive care units were prepared in 9 (9/17, 52.9%), 5 (5/17, 29.4%), 15 (15/16, 93.7%), and 4 (4/16, 25.0%) hospitals, respectively. Except for one hospital that newly made negative pressure room for SARS, single or multi-bed rooms without airborne infection control were used in all the other hospitals. The personal precaution principles were kept quite well in general wards. Before the designation of SARS hospital by the public health department prior evalution to see if the hospital was suitable for managing SARS was conducted in only 1 (1/12, 8.3%) hospital. The results of laboratory diagnosis were reported back in 1 (1/15, 6.6%) hospital. Conclusions : The isolation facilities which can control airborne infection were almost deficient not only for SARS but also for other respiratory transmissible diseases. For the infection control of transmissible diseases including SARS, more investment is needed on medical facilities and comprehensive support from the public health department required.

지역사회 연계를 고려한 진주종합경기장 활용방안 연구

강석진(Kang, Seok-Jin),안재우(Ahn, Jae-Woo),김지희(Kim, Ji-Hee),이현진(Lee, Hyun-Jin) 대한건축학회 2013 대한건축학회 학술발표대회 논문집 Vol.33 No.2

비탈극성 근육이완제의 전투여 효과의 비교 관찰

우현제,김익수,최세진 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.2

Succlnycholine (1 mg/kg, 1.5 mg/kg) without pretreatment or succinylcholine (1 mg/kg, 1.5 mg/kg) with pretreatment by pancuronium (bolus 1 mg, 2 mg), and d-tubocurarine (3 mg, 6 mg bolus) were given to patients of relatively same physical status (ASA classification class Ⅰ, Ⅱ) at induction of general anesthesia to determine the degree of muscle fasciculation, vocal cord paralysis, onset and extnt of muscle pain in each group. The result are summarized as follows 1. Appearance of muscle fasciculation was significantly decreased with pretreatment of non-depolarizing muscle relaxant. (p<0.005) 2. The depressing effect of muscle fasciculation was relatively same in each dosage of non-depolarizing muscle relaxant, thus there is no statistic difference. 3. Degree of vocal cord paralysis was predominant in group of succinylcholine alone used. Degree of vocal cord palaysis was slightly decreased by pretreatment of non-depolarizing muscle relaxant, but comparision with control group, there is no statistic difference (p>0.05) 4. The reduced effect of vocal cord paralysis was relatively same in each dosage of non-depolarizing muscle relaxant, thus there is no statistical difference. (p>0.05) 5. Appearance of muscle pain was decreased by pretreatment of non-depolarizing muscle relaxant, especially pretreatment of pancuronium 2 mg bolus significantly decreased post-operative muscle pain. (When succinyline 1 mg/kg administration : 0.025<p<0.05, when succinylcholine 1.5 mg/kg administration: 0.01<p<0.025).

단중족골중에서 자가 장골 이식후 이식골의 운명과 환자의 만족도

우상현,박진철 大韓成形外科學會 2002 Archives of Plastic Surgery Vol.29 No.6

Pyridostigmine과 Neostigmine의 골격근이완계 길항 효과에 관한 연구

우현제,신용섭,김태관,이정은,최세진 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.2

This present study was undertaken to observe the effects of pyridostigmine and neostigmine of heart rate and it's antagonistic effect on pancuronium in 62 patients during general anesthesia. We obserived the effect of pyridostigmine and neostigmine with atropine and glycopyrrolate of heart rate, appearance of arrhythmia, time required for returning of spontaneous breath an sustained tetanic response and effect on the amount of secretion in oral cavity and trachea. The result are as follows: 1. The incidence of bradycardia was the highest in the group of atropine and neostigmine i. v. administration together, and the lowest in the group of glycopyrrolate and pyridostigmine i. v. administration together. 2. The incidence of tachycardia was the highest in the group of atropine and pyridostigmine i. v. adminstration together, and the least in the group of glycopyrrolate and pyridostigmine i. v. administration together. 3. Among the experimental cases, only one showed arrhythmia which is PVD. 4. The time required for returning of spontaneous respiration was 4.6-6.1 minutes range in all groups. 5. The group of glycopyrrolate administrated showed the greates antisialogue effect.

Effects of Pilger Rolling and Heat Treatment on Microstructure and Corrosion Resistance of Ag-electroplated 304 Stainless Steel Tubes

Hyun Park,Woo‑Jin Lee,Jae‑Han Son,Han‑Kyun Shin,Sung‑Kyu Hong,Hyo‑Jong Lee 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.8

We introduce a cost-effective method that combines electroplating with metal cold working processes to manufacture compositetubes with robust corrosion resistance. First, a 3-step electroplating process was developed to form an adhesive anduniform Ag coating on the outer wall of a 304 stainless steel tube. The process consisted of a Ni-strike step for removing thepassivation layer of the initial 304 tube, Cu deposition for smoothing the surface by adding a buffer layer, and Ag deposition. To reinforce the interfacial adhesion between the electroplated layers and the 304 tube and increase the area of the Ag coating,a pilger rolling or pilger rolling + heat-treatment process was performed after the Ag electroplating process. Scanningelectron microscopy of the composite tubes after each process indicated that the as-deposited Ag coating changed fromhaving a rough to smooth surface after only pilger rolling and with additional heat treatment. Electron backscatter diffractionanalysis of the microstructures and textures of the tubes revealed that dynamic recrystallization occurred extensively duringpilger rolling, resulting in the formation of a relatively defect-free grain structure without heat treatment. Furthermore,electrochemical polarization curves determined that the Ag-coated composite tubes are superior to the uncoated 304 tubein terms of corrosion resistance under Cl−atmosphere, owing to the formation of a AgCl passivation layer during testing. Surface analysis of the composite tubes suggests that the properties of the AgCl passivation layer are related to the grainsize of the Ag coating and the density of plastic-deformation-induced defects.

Modified Hogan 법을 이용한 범인두 부전 환자의 인두피판 성형술

진우정,정기범,이현상,고승오 大韓顎顔面成形再建外科學會 1996 Maxillofacial Plastic Reconstructive Surgery Vol.18 No.4

A competent velopharyngeal sphincter is essential for intelligible speech. If the velopharyngeal incompetence exist, the seal will not be complete during speech, with a resultant hypernasal speech quality. The patient with velopharyngeal incompetence(VPI) may develope other compensatory speech problems. There are many approaches available to correct veolpharyngeal incompetence, which include speech therapy, push back palatorrhaphy, pharyngeal wall implants and pharyngoplasty. This is cases report of velopharyngeal incompetence, which were successfully treated by superiorly based pharyngeal flap, covered with splitted hinge flap of nasal lining mucosa of the soft palate, named modified Hogan method. The advantages of this method are precision in the approximation of the flap due to pentagonal shaped flap design, good flood supply due to omission of the midsagittal incision on nasal lining mucosa, and simplicity than Hogan method.

初等學校 校庭內 生態園 造成技法 : With a Special Reference to Hu-am Elementary School in Yong-san gu, Seoul 서울 龍山區 厚岩 初等學校 事例를 중심으로

진시현,심우경,이광우 韓國植物ㆍ人間ㆍ環境學會 2000 인간식물환경학회지 Vol.3 No.1

이 논문의 연구목적은 초등학교내에 생태원을 조성함으로써 아이들에게 자연과의 접촉기회를 제공하고자 실시한 바, 그 결과는 다음과 같다. 1. 초등학교 교과서에 나타나는 모든 동, 식물의 종류 초등학교의 교과서 분석 경과 274종의 식물종과 227종의 동물종이 나타났으며, 가장 높은 빈도수를 나타낸 곤충은 나비 78회, 잠자리 64회, 개미 51회, 벌 41회, 그리고 매미가 31회의 순으로 나타났다. 이는 교과서별 학년별로 빈도수를 조사한 결과와도 비슷한 양상을 나타낸 것이다. 초등학교 교과서 중 자연과 과목의 교육내용을 살펴본 결과, 1∼2학년 과정에서는 학교내의 뜰과 화단의 4계절 변화를 관찰하고 3∼6학년 과정에서는 생태계 전반에 걸친 세부적인 내용을 공부하도록 교육내용이 설정되었음을 알 수 있었다. 2. 용산구에 있는 15개의 초등학교의 옥외환경 조사 용산구에는 15개 학교가 있고, 그들의 옥외환경의 형태는 교재원과 생산원의 두 가지 종류로 나타났다. 대부분의 학교가 이 두 가지 형태로 나타났으나 남정, 보광, 원효로, 한강 초등학교는 생태원의 시설중 소동물 사육장 시설이 없었고, 교재원의 시설도 빈약하였다. 사례지역인 후암 초등학교는 도시의 중심부에 위치해 있는 다른 초등학교와는 달리 풍부한 생물상을 가지고 있는 남산에 인접하고 있기 때문에 생태원으로서의 기능성이 있는 것으로 보여진다. 3. 사례연구와 생태원 조성계획 생태원의 조성방법으로서 우선, 사례지인 후암 초등학교 부지의 현황조사를 하였고, 두 번째로 4가지 곤충(나비, 잠자리, 벌, 매미)들을 유치하기 위한 식물들을 선별하기 위해 이들이 식이식물로 조사된 식물들 중에서 교과서에 나오고 사례지역에 적합하며 내공해성을 가지며, 자생종이 식물들을 선별한 결과, 나비, 잠자리, 새를 유치하기 위한 식이식물로 각각 44, 17, 16종이 선별되었다. 또한 이 학교의 건물이 도로와 맞닿는 점을 감안하여 건물옥상에 생물서식지를 조성함으로서 남산으로부터의 풍부한 생물종의 유입을 유도하고, 더 나아가 학교 내에 조성되는 생태원으로 자연스럽게 유도하고자 하였다. 따라서 옥상 위의 생물서식공간은 수변공간계획과 식재계획을 통하여 곤충류와 조류뿐만 아니라 다양한 생물종이 서식되도록 하였고, 이를 통해 초등학교 학생들은 자연과의 친밀한 접촉기회를 가지게 될 것이다. The purpose of this study was to offer the opportunity of contact with the nature to urban children by the creation of the biotope in the elementary school ground. The results for this study were as follow: 1. The kinds of animals and plants the elementary school textbooks were 275 species and 227 animals. The frequencies of insect species in the elementary school textbook were the butterfly(75), dragonfly(64), ant(51), bee(41), and cicada(31) in order. These results were analogous to the numbers in each textbook and grade. In the 'Nature' textbook of the elementary school, there were educational contents to observe the ground and garden in 1, 2 grade courses and to study the detail of ecosystem in 3-6 grade course. 2. Inventory of the out-door environments of 15 elementary schools in Young-san Gu, Seoul. The number of total elementary school in Young-san Gu were fifteen, which had two garden types appeared as nature study and experimental farm. Two types of gardens were appeared mostly in all the elementary school but Nam-jung, Bu-kwang, Wonhyo-ro, Sin-swang, and Han-gang elementary school didn't have the experimental farms and also have the poor facilities of the nature study gardens. Huam elementary school had the possibility of the biotope creation because it was connected to Namsan park which posseses the abundant biota in contrast to the others in the center of the city. 3. Case study and biotope planning. To create the biotope, the site analysis was carred out and then plant species for inducing 4 kinds of insect (butterfly, dragonfly, bee, and cicada) were selected under the criteria as pollution-resistant, the school tree and flower, tolerance, and original habitat. Consequently, the numbers of plants for inducing butterflies, dragonflies and birds were 44, 17, 16 species separately. Also, it was planned to the induce abundant biota to building's roof as stepping stone from Namsan. These two biotopes were planned to be a habitat of various biota in addition of the water pond and the planting plan. Through the biotope created by this plan, the students of the elementary school will have more opportunity of contact with the nature.

항정신병약물을 처음으로 사용하는 초발 정신증 환자에서 나타나는 체중 및 대사성 지표의 변화 : 후향적 연구

김우진,심주철,공보금,강제욱,문정준,김정은,김민걸,박민경,김성진,김현정,정도운 대한생물치료정신의학회 2011 생물치료정신의학 Vol.17 No.2

Objectives:This study was to identify weight & metabolic changes in first-episode psychotic patients with antipsychotics use and investigate the differences of weight & metabolic changes between first-episode psychotic patients and controls with antipsychotics use. Method:In this retrospective study, twenty eight first-episode psychotic patients and twenty eight controls with schizophrenia, schizoaffective or bipolar disorder defined by DSM-IV criteria were included. Information about demographic and clinical characteristics of the subjects was gathered from the medical records. Also body weight, sBP, dBP, fasting glucose, lipid profile and WBC count were evaluated before and after antipsychotics use. Rogistic regression was conducted to assess factors associated with weight gain. Results:First-episode psychotic patients showed more weight and BMI changes than controls after antipsychotics use, and these changes continue over 12 months. On the other hand, there were no significant factors associated with weight gain. Conclusion:The results of present study suggest that antispychotics is one of the major causes inducing weight gain of psychotic patients and antipsychotics-induced weight gain is more vulnerable to drug-naive first-episode psychotic patients.