김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

Holstein種의 血液化學値에 關한 硏究 : -Ⅰ. 成牝牛의 血液化學値- -Ⅰ. Blood Chemical Values of Adult Cows -

李載洪,林貞澤,韓邦根,金宇權 全南大學校 農漁村開發硏究所 1981 農業科學技術硏究 Vol.16 No.1

國內에서 飼育되고 있는 Holstein種成牝牛의 血液化學値를 얻고자 全南地域에서 飼育되고 있는 外觀上 健康하다고 認定된 經産牛 385頭를 對象으로 血液化學値를 調査檢討하여 다음과 같은 結論을 얻었다. 1. 平均血淸TP量은 7.35(5.10∼10.20)g/100㎖이었으며 年令의 增加에 따라서 漸漸 증가하는 傾向이 있었다. 2. 平均血淸Alb量은 3.51(2.50∼4.40)g/100㎖이었으며 年令別 差異는 認定할수 없었다. 3. 平均血淸Glb量은 3.84(1.40∼7.20)g/100㎖이었으며 年令의 增加에 따라서 漸漸 증가하는 傾向이 있었다. 4. 平均血淸 A/G比는 0.91(0.43∼2.64)이었으며 年令의 增加에 따라서 漸漸 증가하는 傾向이 있었다. 5. 平均血淸 cholesterol量은 179.38(46.4∼336.0)g/100㎖이었으며 年令의 增加에 따라 增加하여 4年牛를 頂点으로 다시 下向의 傾向을 나타내고 있었다. 6. 平均血淸 glucose量은 39.38(27.0∼57.3)㎎/100㎖로 낮았으며 年令別로 特徵的인 것은 發見할수 없었다. 7. 平均血淸 magnesium量은 2.72(0.20∼8.46)㎎/100㎖이었으며 老令牛에서 magnesium의 減少가 顯著하였다. 8. 平均血淸 calcium量은 8.69(6.34∼16.83)㎎/100㎖로 全般的으로 낮은 량이었으며 年令別 差異는 認定할수 없었다. 9. 平均血淸無機燐量은 5.88(3.50∼9.76)㎎/100㎖이었으며 年令別로 特徵的인 것은 發見할수 없었다. 10. 平均血淸Ca/P比는 1.48(0.68∼2.64)이었으며 年令別로 特徵的인 것은 發見할수 없었다. 11. 平均血淸 potassium量은 18.95(13.69∼24.24)㎎/100㎖이었으며 年令別 差異는 認定할수 없었다. 12. 平均血淸 sodium量은 315.90(282.86∼361.05)10.20)㎎/100㎖이었으며 年令別로 特徵的인 것은 發見할수 없었다. 13. 平均血淸 chloride量은 354.64(299.97∼361.05)㎎/100㎖이었으며 年令別 差異点은 發見할수 없었다. The present study was conducted to determine the blood chemical values of adult Holstein cows in Korea. Samples of blood were taken from 385 healthy cows in Chonnam province, and the results obtained are summarized as follows. 1. Mean serum totalprotein content was 7.35(5.10∼10.20)g/100㎖;it in creased with age. 2. Mean serum albumin content was 3.51(1.40∼4.40)g/100㎖;no differences were found between age groups in the serum albumin content. 3. Mean serum globulin content was 3.84(1.40∼7.20)g/100㎖; it increased with age. 4. Mean serum albumin/globulin ratio was 0.91(0.43∼2.64);it decreased with age. 5. Mean serum glucose concentration was 179.38(46.4∼336.0)g/100㎖;it increased up to 4 years of age and thereafter it decreased with age. 6. Mean serum glucose concentration was 39.38(27.0∼57.3)㎎/100㎖, which was low compared with other results. No differences were found in the glucose concentration between age groups. 7. Mean serum magnesium concentration was 2.72(0.20∼8.46)㎎/100㎖;it decreased significantly in older cows. 8. Mean serum calcium concentration was 8.69(6.34∼16.83)㎎/100㎖, which was low compared with other results. No differences were found in the calcium concentrati on between age groups. 9. Mean serum inorganic phosphate concentration was 5.88(3.50∼9.76)㎎/100㎖;no differences were found in the inorganic phosphate concentration between age groups. 10. Mean serum calcium/phosphate(Ca/P) ratio was 1.48(0.68∼2.64);no differences were found in the Ca/P ratio between age groups. 11. Mean serum potassium concentration was 18.95(13.69∼24.24)㎎/100㎖;no differences were found in the potassium concentration between age groups. 12. Mean serum sodium concentration was 315.90(282.86∼361.05)10.20)㎎/100㎖;no differences were found in the sodium concentration between age groups. 13. Mean serum chloride concentration was 354.64(299.97∼361.05)㎎/100㎖;no differences were found in the cholride concentration between age groups.

Ehrlich 복수암 세포의 지질대사에 관한 연구

이희성,하창우,이근배 중앙대학교 의과대학 의과학연구소 1977 中央醫大誌 Vol.2 No.1

We have studied on lipids of Ehrlich ascites tumor cells (EATC). Lipids were extracted by Kennedy and Kanfer(1964) method and lipoproteins were separated by paper electrophoresis. For the identification and assay of lipid extracts, thin-layer chromatography was employed. The incorporation of ^32P-orthophosphate into phospholipids of EATC was also observed. The results obtained were summarized as follows; 1. The total lipid content of EATC was 47.15 ㎎ per g wet weight; of this 32.2㎎ per g was neutral lipid and 14.95 ㎎ per g was phospholipid. 2. The amounts of neutral lipid fractions of EATC, i.e., free cholesterol, esterified cholesterol, mono-, di-, triglyceride and free fatty acid were 2.06㎎, 1.75㎎, 4.71㎎, 7.15㎎, 10.74㎎ and 2.21㎎, respectively. The amount of triglyceride of EATC was similar to those of normal mouse liver. 3. Phospholipid composition of EATC was phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine and unidentified phospholipid, and contents of the individual phospholipids showed that the over-all composition were very similar to normal mouse liver. 4. Lipoprotein fractions of EATC were chylomicron, β-lipoprotein, α-lipoprotein, and prealbumin. Chylomicron fraction contained a larger amount of lipid and α-lipoprotein fraction contained a smaller amount of lipid. 5. The incorporation of ^32P into phospholipids reached a maximum 5 minutes after incubation.

시간 경과에 따른 부가중합형 실리콘 인상재의 수소 발생

이근우,김경남,고영무 大韓齒科器材學會 1995 대한치과재료학회지 Vol.22 No.1

Clinical reports have indicated that there occurs many bubbles on the surface of the gypsum model because of the hydrogen release from the addition silicone impression materials. Recently the products containing hydrogen absorber have been being developed but not perfect yet. In this study, 4 type Ⅲ dental stones(New fujirock ; G.C., New Plastone ; G.C., MG dental stone ; Maruishi, Suprastone ; Kerr) was poured into the impression of detail-reproducing test block taken using addition-type silicones (Correct VPS ; J/P, Exafine ; G.C., Express ; 3M, Extrude ; Kerr, Provil ; Bayer, Reprosil ; Dentsply) with the intervals of 0, 15, 30, 45, 60, 90 and 120 minutes. Gypsum model was removed from the impressions and the number of bubbles on the surface of casts was counted after 30 min. Ordinal numbers were given to the ranges of the number of bubbles like this, Score 0 ; 0, 1 ; 1∼10, 2 ; 11∼30, 3 ; 31∼60, 4 ; 61∼100, 5 ; 101∼200, 6 ; 201∼400, 7 ; 401 or more. There was no bubble in Correct VPS impression poured immediately in MG dental stone and Extrude impression poured immediately in New Fujirock, New Plastone, and MG dental stone. Significant bubbles were appeared in Provil until 15 minutes(Score 1∼3), Reprosil until 30 minutes(Score 1∼2), and Express until 45 minutes(Score 1∼2) in all gypsum models(p<0.05). Express impression poured in Suprastone(Score 2) and New Plastone(Score 4) had bubbles until 120 minutes(p<0.05). On the basis of this study it can be concluded that not only hydrogen release but also compatibility with gypsum should be considered when using addition silicones.

한국산 사료의 카드뮴 함량에 관한 조사연구

이현범,이근우 경북대학교 1990 새마을 硏究論叢 Vol.11 No.-

Present study was undertaken in order to evaluate the cadmium content of various feedstuffs produced in Korea. Sixty-two samples were collected from 6 regions. All of the samples were washed, dry-ashed and analyzed for cadmium content using atomic absorption spectrophotometer. The results obtained were summrized as follows: 1) The cadmium content in ㎍/g of pasture plants were measured with the following mean values. clover:7.10㎍/g, sagebrush:1.88㎍/g, Koreangrass:1.23㎍g/g. 2) The cadmium content in ㎍/g of agriculture-byproducts were measured with the following mean values. maizestalk:3.04ug/g, soybeanpod:0.83㎍/g, rice straw:0.68㎍/g, but rice hull not detected. 3) Twenty-eight samples(45%) out of 62 samples detected cadmium and regional mean values were Kangwondo:2.85㎍/g, Kyungsangdo:3.01㎍/g, Chungchyugdo:1.72㎍/g, Kyungkido:0.62㎍/g, Chonrado:2.24㎍/g, Jejudo:0.51㎍/g.

소각장별 비산재 중의 중금속 용출 특성 및 존재형태

이우근,김준수,김진범 江原大學校 附設 環境硏究所 1998 環境硏究 Vol.15 No.-

본 연구는 도시폐기물 소각시설에서 발생되는 비산재 중에 함유된 중금속으 존재형태 및 용출 툭성을 알아보기 위해 수행되었다. 용출 실험은 KSLT, TCLP, MALT법을 이용하였고 중금속의 존재형태별 분포에는 다음과 같이 5가지 형태로 구분하였다.; exchangeable, carbonate, reducible, organic, residual fraction.용출 심험 결과에 의하면 pH 4로 일정하게 유지한MALT법에 의한 중금속 용출량이 가장 높 This study was carried out to estimate the fractional composition and leaching properties of heavy metals in fly ash generated from three municipal solid waste incinerators(MSWI). Three leaching tests are used in this work. These are Korea Standard Leaching Test(KSLT)), Toxicity Characteristic Leaching Procedure (TCLP), Maximum Availability Leaching Test(MALT) method. The fractional composition of heavy metals is classified into five fractions; exchangeable, carbonate, reducible, organic, residual fraction. According to the leaching tests, it seems that the highest amounts of heavy metals are leached by MALT method in which pH of 4.0 maintains constantly. From the results of the fractional composition of heavy metals, above 45% of Cd is leached by the change of pH or/and concentrations at I and J incinerators. And 91% of Cd is extracted under the reducible environment at B incinerator. But Cu may be leached under the strong acidic environment. The amount of Cu at B incinerator is less leached than that of I and J incinerators due to high residual fraction. About 35% of Ni in fly ashes at three incinerators exists in reducible fraction. It is probably leached under the reducible environment. Pb may be leached under the weak acidic environment at I and J incinerators, but in case of S incinerator leached under the strong acidic environment.

의사결정지원시스템에서 직관적이고 사용자 친숙한 모델 해결을 위한 모델과 솔버의 유연한 통합에 대한 연구

이근우,허순영 한국경영과학회 2005 韓國經營科學會誌 Vol.30 No.1

Research in the decision sciences has continued to develop a variety of mathematical models as well as software tools supporting corporate decision-making. Yet, in spite of their potential usefulness, the models are little used in real-world decision making since the model solution processes are too complex for ordinary users to get accustomed. This paper proposes an intelligent and flexible model-solver integration framework that enables the suer to solve decision problems using multiple models and solvers without having precise knowledge of the model-solution processes. Specifically, for intuitive model-solution, the framework enables a decision support system to suggest the compatible solvers of a model autonomously without direct user intervention and to solve the model by matching the model and solver parameters intelligently without any serious conflicts. Thus, the framework would improve the productivity of institutional model solving tasks by relieving the user from the burden of learning model and solver semantics requiring considerable time and efforts.

Earth Anchor의 인발저항력에 관한 연구

이유근,김우중,윤용철 진주산업대학교 1994 論文集 Vol.33 No.-

This paper describes the results of an experimental consideration on the uplift capacity of the earth anchor. The earth anchor used in pull-out test is the cylindrical steel with plate and eight earth pressure cells(No.1~No.8). The plate diameter is 120㎜ and embedment ratios(L/B; L=length of embedment, B=diameter) are 1, 2 or 3, Also, we investigated surface displacement of the test ground using two gap sensors. Pull-out test were conducted for dry sand of soil placed at a density of loose. From the results, the following conclusions were drawn ; Uplift capacity of anchor becomes larger due to the increase in the L/B. The earth pressure act upon the upside of the anchor plate is different in according to place of earth pressure cell and L/B. That is to say, the earth pressure of No.1 or No.2 (earth pressure cell) increases as time goes by until the anchor pull-out completely, but the earth pressure of No.3(L/B≤2) becomes smaller No.1 and No.2 after the rate of increase of the earth pressure decreased by boundary a point of time. The intersecting point of the ground(sand) surface and the end of shear bed is about 15㎝(L/B=1), 21㎝(L/B=2) or 27㎝(L/B=3) by distance from anchor center.

지문과 정신분열증

우숙희,정국동,최송표,조근자,양은진,김수일,박경란,이영호,김원식 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Fingerprint patterns of 333 schizophreniacs who met with the diagnostic criterias of International Pilot Study of Schizophrenia except items associated with chronicity in exclusion criteria were studied, and the following results were obtained. 1. The frequency distribution of the fingerprint patterns in the 333 schizophreniacs was ulnar loop, whorl, twinned loop, arch, radial loop in order in both control and schizophrenia groups. 2. Finger ridge counts of both sexes were decreased significantly in schizophrenia group. 3. Dissociation of fingerprint pattern in the schizophrenia group was 51.1%, which was far greater than 6.25% of control group. Number of dissociation was greatest in the thumb, followed by index, middle, ring, and little finger in order. According to the above results, it is considered that both the number of finger ridges and degree of dissociation of fingerprint patterns were closely related with schizophrenia. Analysis of fingerprints and palmprints with the aid of chromosomal analysis would contribute the early diagnosis and prevention of schizophrenia.

Holstein種의 血液像에 關한 硏究 : Ⅰ. 成牝牛의 血液像 Ⅰ. Hematologic Values of Adult Cows

李載洪,金宇權,林貞澤,韓邦根 全南大學校 農漁村開發硏究所 1980 農業科學技術硏究 Vol.15 No.-

國內에서 飼育되고 있는 Holstein種 成牧牛의 血液上의 標準値를 얻고저 全南地域에서 飼育되고 있는 外見上 建康하다고 認定된 經産牛 670頭를 對象으로 血液上을 調査 檢討하여 다음과 같은結論을 얻었다. 1. 平均 RBC數의 現實値는 6.06(3.20~9.74)x 10??/㎕이었으나 正常値는 6.35(5.00~8.95)x10??/㎕이었으며 年令의 增加에 따라서 漸漸 減少하는 傾向이 있었다. 2. 平均 Hb量의 現實値는 9.42(5.3~18.4)g/100ml 이었으나 正常値는 10.11(8.0~13.9)g/100ml 이었으며 年令의 增加에 따라서 漸漸 減少하는 傾向이 있었다. 3. 平均 PVC値의 現實値는 31.04(19~55)g/100ml 이었으나 正常値는 32.30(27~42)g/100ml 이었으며 年令의 增加에 따라서 漸漸 減少하는 傾向이 있었다. 4. 平均 MCV의 現實値는 51.22(29.77~86.57) fl 이었으나 50.87(41.56~62.00)fl로 現實値보다 낮았다. 5. 平均 MCH의 現實値는 15.54(8.21~28.96)pg 이었으나 正常値는 15.92(13.00~20.94)pg로 現實値보다 높았다. 6. 平均 MCHC의 現實値는 30.35(17.35~ 53.57)g/100ml이었으나 正常値는 31.30(28.00~36.97)g/100ml로 現實値보다 높았다. 7. 平均 WBC수의 現實値는 10,408(3,200~31,600)/㎕ 이었으나 正常値는 9,233(4,810~14,000)/㎕이었으며 年令의 增加에 따라서 漸漸 減少하는 傾向이 있었다. 8. WBC 감별계수의 正常値는 임파구가 66.00(50~80), 單核球가 3.35(0~9), 好中球가 26.06(10~38), 好酸球가 4.24(0~11)그리고 好鹽基球가 0.35(0~2)% 이었으며 現實値와 비슷하였다. 9. 淋巴球는 年令의 增加에 따라서 漸漸 減少하고 있었으나 單核球와 好中球는 年令의 增加에 따라서 漸漸 減少하고 있었다. 10. 平均 血小板數의 現實値는 243,645(93,000∼57,000)/㎕이었고 正常値는 248,453(116,000~445,000)/㎕으로 現實値와 거의 비슷하였으며 年令別 差異는 認定할 수 없었다. In order to get the normal blood values, blood samples were taken from 670 healthy Holstein cows in Chonnam province. The result obtained in the present experiment are summarized as below. 1. Determined average red blood cell count was 6.06(3.20∼9.74)x 10??/㎕, whereas average red blood cell count obtained from the results in the normal range(normal) was 6.35(5.00∼8.95)x10??/㎕. The red blood cell count tended to decrease with age. 2. Determined average hemoglobin content was 9.42(5.3∼18.4)g/100ml. whereas normal hemoglobin content was 10.11(8.0∼13.9)g/100ml. The hemoglobin content tended to decrease with age. 3. Determined average packed cell volume(PCV) was 31.04(19∼55)g/100ml, while normal PCV was 32.30(27∼42)g/100ml. The PCV appeared to decrease with age. 4. Determined mean corpuscular volume(MCV) was 51.22(29.77~86.57) fl. Normal MCV was 50.87(41.56∼62.00)fl, which was lower than the determined MCV. 5. Determined mean corpuscular hemoglobin(MCH) was 15.54(8.21∼28.96)pg. Normal MCH was 15.92(13.00∼20.94)pg, which was higher than the determined MCH. 6. Determined mean corpuscular hemoglobin concentration(MCHC)was 30.35(17.35∼ 53.57)g/100ml. Normal MCHC was 31.30(28.00∼36.97)g/100ml, which was higher than the determined MCHC. 7. Determined average white blood cell(WBC) count was 10,408(3,200∼31,600)/㎕, whereas normal WBC count was 9,233(4,810∼14,000)/㎕. The WBC count appeared to decrease with age. 8. In the normal WBC differential count, the values were lymphocyte 66.00(50∼80)%, monocyte 3.35(0∼9)%, neutrophil 26.06(10∼38)%, eosinophil 4.24(0∼11)% and basophil 0.35(0∼2)%, respectively. No differences were found between the determined and normal values. 9. Lymphocyte count tended to decrease with age, while the reverse was the case with monocyte and neutrophil count. 10. Determined average platelet count was 243,645(93,000∼570,000)/㎕, whereas normal platelet count was 248,453(116,000∼445,000)/㎕. The platelet count did not change with age.