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      • KCI등재

        (E)-2,4-Bis(p-hydroxyphenyl)-2-butenal enhanced TRAIL-induced apoptosis in ovarian cancer cells through downregulation of NF-jB/STAT3 pathway

        조승희,박미희,이희범,백명기,성하창,장희원,김주환,정헌상,한상배,홍진태 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.5

        Ovarian cancer is a cancerous growth arisingfrom the ovary and with poor prognosis that usually haveresistant to all currently available treatments. Whether (E)-2,4-bis(p-hydroxyphenyl)-2-butenal (butenal) synthesizedby Maillard reaction from fructose–tyrosine, has potentialtherapeutic activity against human ovarian cancer wasinvestigated using two ovarian cancer cell lines (PA-1, SKOV-3). We found that butenal could inhibit NF-jB/STAT3activity, thereby inducing apoptotic cell death of ovariancancer cells. We treated with several concentration ofbutenal each cell line differently (PA-1; 5, 10 and 15 lg/ml,SK-OV-3; 10, 20 and 30 lg/ml). First, ovarian cancer celllines exhibited constitutively active NF-jB, and treatmentwith butenal abolished this activation as indicated by DNAbinding activity. Second, butenal suppressed activation ofsignal transducer and activator of transcription-3 as indicatedby decreased phosphorylation and inhibition of Januskinase-2 phosphorylation. Third, butenal induced expressionof pro-apoptotic proteins such as proteolytic cleavageof PARP, Bax and activation of caspase-3, -8 and -9. Lastly, combination of butenal and TRAIL causesenhanced induction of apoptosis. Overall, our resultsindicate that butenal mediates its anti-proliferative andapoptotic effects through activation of multiple cell signalingpathways and enhances the TRAIL-induced apoptosis. These data suggested that butenal may be a potentialanti-cancer agent in ovarian cancer.

      • KCI등재

        병원간호사의 VDT증후군 영향요인

        조승희,조규영 한국자료분석학회 2016 Journal of the Korean Data Analysis Society Vol.18 No.5

        The purpose of this study examined the factors that affect VDT (visual display terminal) syndrome. The subjects of this study were nurses who worked at 5 general hospital with capacities of over 300 beds in city B. The data were collected from 170 hospital nurses, who had at least 3 months experience of VDT work. The collected data were analyzed by frequency, percentage, mean and standard deviation, t-test, ANOVA, Pearson’s correlation coefficients. and multiple regression via the SPSS/WIN 20.0 program. As a result of analyzing the severity of VDT syndrome according to subjects’ socio-demographic and work-related characteristics, the factors job satisfaction, VDT work time per day, continuous VDT work time per session and posture during VDT work were statistically significant. VDT syndrome averaged at 1.56±.74 out of 4 points. Job stress was found to be correlated with VDT syndrome, and health promoting behavior were found to be negatively correlated with VDT syndrome. Factors that affected VDT syndrome for the subjects were job satisfaction, continuous VDT working time per session, posture during VDT work, job stress, health promoting behaviors. The explanatory power was 26.7%. In conclusion, there factors should be considered when developing the program preventive strategy to decrease VDT syndrome. 본 연구는 간호사들의 VDT증후군에 영향을 미치는 요인을 파악하기 위해 수행되었다. 연구대상자는 B광역시에 소재하는 300병상이상 규모의 종합병원 5곳에서 근무하며 3개월 이상 VDT 작업을 한 간호사 170명이었으며, 자가 보고식 설문지를 통해 직무스트레스, 건강증진행위와 VDT증후군을 측정하였다. 자료 분석은 t-test, ANOVA, Pearson 상관계수, 다중회귀분석을 이용하였다. 본 연구 결과, 연구대상자의 인구사회학적 특성에 따른 VDT증후군 정도는 직업만족도, 1일 VDT 작업시간, 1회 VDT 연속작업시간, VDT 작업 시 자세에서 통계적으로 유의하였다. 연구대상자의 직무스트레스 정도는 5점 만점에 3.53점±.48점, 건강증진행위 수행정도는 2.14점±.36점, VDT증후군 자각증상정도는 4점 만점에 1.56±.74점이었다. 직무스트레스와 VDT증후군은 양(+)의 상관관계가 있는 것으로 나타났고, 건강증진행위와는 음(-)의 상관관계가 있는 것으로 나타나 직무스트레스가 높을수록, 건강증진행위 수행정도가 낮을수록 VDT증후군 증상이 높은 것으로 나타났다. VDT증후군에 영향을 미치는 요인은 직업만족도, 1회 VDT 연속작업시간, VDT 작업 시 자세, 직무스트레스, 건강증진행위로 설명력은 26.7%이었다. 따라서 간호사의 VDT증후군개선을 위하여 건강증진행위와 직업만족도를 향상시키고, 직무스트레스를 감소시킬 수 있는 교육프로그램을 개발하고 활성화할 수 있는 방안이 필요하다.

      • KCI등재

        영세농가 콩 재배지의 Soybean mosaic virus, Soybean yellow mottle mosaic virus 및Soybean yellow common mosaic virus 병 발생 조사

        조승희,임현섭,김정규,이미가,서은영,임승모,홍석명,문제선,John Hammond 한국식물병리학회 2013 식물병연구 Vol.19 No.4

        Soybean yellow mottle mosaic virus (SYMMV) and Soybean yellow common mosaic virus (SYCMV) wererecently isolated in Korea, and it has not been reported how two viruses were dispersed in Korea. In 2012, weperformed nationwide survey in subsistence soybean farming. Suspicious virus-infected infected leave werecollected from the field and a total of 682 soybean tissue samples were assayed by RT-PCR using triplexprimers detecting SYMMV, SYCMV, and Soybean mosaic virus (SMV). On hundred two samples showedSMV positive, and SYMMV and SYCMV were detected in 116 and 17 tissue samples, respectively. No sampleshowed double infection of SYMMV and SYCMV, but there were double infection tissues indicating twoviruses positive of SMV plus SYMMV (5 tissue samples) and SMV plus SYCMV (1 tissue sample). Throughthis first subsistence soybean farming field survey, we assumed soybean viruses were originated from homeseed production managed by farmer. Thus, in order to prevent possible seed transmission and furtherdamage caused by virus transmission, virus-free commercial soybean seeds are recommended to be planted. Soybean yellow mottle mosaic virus(SYMMV)와 Soybeanyellow common mosaic virus(SYCMV) 병 발생은 최근에우리나라에 보고되었지만 두 바이러스 병의 전국적인 분포에 대한 조사가 이루어지지 않아 본 연구팀은 2012년에 영세 농가를 중심으로 전국적인 조사를 실시하였다. 전국에 걸쳐 채집한 682점의 시료를 SYMMV, SYCMV,그리고 Soybean mosaic virus(SMV) 특이 프라이머를 이용하여 진단한 결과, SMV 102점, SYMMV 116점,SYCMV는 17점이 PCR 양성으로 나타났다. SYMMV와SYCMV의 복합감염은 검출되지 않았으나, SMV와SYMMV 혹은 SMV와 SYCMV 복합감염은 각각 1건과5건이 확인되었다. 처음으로 실시한 영세농가의 콩 바이러스 병 조사를 근거로 추정할 때, 바이러스의 발생은자가 채종으로 인한 종자전염으로부터 발생되었을 것으로 추정된다. 따라서 바이러스 병의 예방을 위해서는 감염종자 관리 및 무병종자 보급이 무엇보다 필요함을 알수 있다.

      • KCI등재

        마음챙김이 여대생의 스트레스에 미치는 영향: PA/NA조절효과를 중심으로 한 예비연구

        조승희,배재홍,신호영,Cho, Seung-Hee,Bae, Jae-Hong,Shin, Ho-Young 한국융합학회 2021 한국융합학회논문지 Vol.12 No.7

        본 연구의 목적은 여대생을 대상으로 마음챙김이 대학생 생활스트레스에 미치는 영향을 알아보고자 하였으며, 두 변수간의 관계에서 정적정서 및 부적정서가 조절효과를 가지는 지 검증하고자 하였다. 이 연구를 위해 K여자대학교 여대생(243명)을 대상으로 마음챙김 주의자각, 정적정서와 부적정서, 대학생 생활스트레스에 대해 설문조사를 실시하고 자료를 수집하였다. 자료분석 결과는 다음과 같았다. 첫째, 여대생의 마음챙김은 대학생 생활스트레스에 영향을 미친 것으로 나타났다. 둘째, 여대생의 마음챙김과 스트레스의 관계에서 정적정서는 조절효과를 보여주지 않았다. 셋째, 여대생의 마음챙김과 스트레스의 관계에서 부적정서는 조절효과를 보여주었다. 결론적으로 여대생의 마음챙김을 통한 주의 자각은 대인관계 스트레스, 당면과제 스트레스를 낮추는 데 도움이 되었으며, 부적정서를 감소시키는 것으로 나타났다. 논의에는 본 연구의 한계점과 향후 연구방향을 논의하였다. The purpose of this study was to examine the effects of mindfulness on the stress of university students' lives, and to verify that Positive Affect & Negative Affect have a moderating effect in the relationship between the two variables. The Mindful Attention Awareness Scale, Positive Affect & Negative Affect Schedule, and the life stress scale for college student were utilized for 243 female university students at K Women's University. First of all, Mindfulness of female university affected the stress of college students' lives. Second, Positive Affect did not have a moderating effect in the relationship between female college student's mindfulness and stress. Third, In the relationship between mindfulness and stress, female college students showed a moderating effect. In conclusion, female university students' awareness of mindfulness helped lower interpersonal stress, current task stress, and decreased Negative Affect. The discussion discussed the limitations of this study and the future direction of the study.

      • KCI등재

        Development of C-Language-based Coding Educational Game for Beginners

        조승희,홍정호,박소영 (사)한국컴퓨터게임학회 2018 한국컴퓨터게임학회논문지 Vol.31 No.3

        In this paper, we propose a C language-based coding educational game for beginners. The proposed coding educational game has the following characteristics. First, the proposed coding educational game is designed on the C language, in order to lead a learner to be able to develop a complex system later. The game consists of 6 stages representing the basis of C language: variables and constants, basic data types and operators, conditional statements, loop statements, functions, and arrays. Second, the proposed coding educational game kindly provides descriptions and hints, so that the learner can play the game without knowledge about C language. Experimental results, show that the learner clears the stage by 85.4% in the PC environment, after the learner tries to submit 5.7 times on average per stage, because the learner can resubmit the code when the submitted code is wrong. Third, the proposed coding educational game was designed as the Web-based game for the learners in various environments. Describing how the learners interact differently in PC environment and mobile environment, experimental results show that the learner feels more comfortable to use PC version than mobile version.

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