Abscisic acid 농도에 따른 밀 종자의 발아와 단백질체의 발현 특성

정재혁,김대욱,황운하,안승현,정한용,이현석,최인배,최경진,윤종탁,윤성중 한국작물학회 2018 한국작물학회지 Vol.63 No.1

This study was conducted to investigate the germination and proteome profile characteristics of wheat seeds treated under various concentrations of abscisic acid (ABA). After-ripening, the seeds of three wheat cultivars (Baegjoong, Keumkang, and Uri) showing different levels of dormancy were used. Germination index and germination rate of the cultivars was higher than 0.95% and 98%, respectively, and these were not significantly different under 0, 10, 30, and 50 μM ABA at 7 d after germination. However, the growth of the shoot and radicle was significantly inhibited at 10, 30, and 50 μM ABA compared to that at 0 μM ABA. Mean ABA content of the embryos of seeds germinated at 0 and 50 μM ABA for 7 d was 0.8 and 269.0 ngmg -1 DW, respectively. Proteins extracted from embryos germinated for 4 d were analyzed by two-dimensional gel electrophoresis, and proteins showing a difference of 1.5-fold or greater in their spot volume relative to that of 0 μM ABA were identified. The expression of four protein spots increased at 50 μM ABA and two protein spots were detected only at 50 μM ABA; these six proteins were all identified as globulin types. Conversely, the expression of three protein spots decreased at 50 μM ABA and were identified as cytosolic glutamine sysnthetase, isocitrate dehydrogenase, and S-adenosylmethionine synthetase 2. In conclusion, ABA did not inhibit the germination rate regardless of pre-harvest sprouting characteristics of the cultivars. However, the growth of the shoot and radicle was significantly inhibited by ABA, most likely through the down regulation of glutamine, methyl group donor, and polyamines biosynthesis, among others, while accompanied by globulin accumulation in the embryos. 휴면성이 다른 백중밀, 금강밀, 우리밀 후숙종자의 ABA 농도에 따른 발아 및 배아에서의 단백질체 발현 특성을 조사한 결과는 다음과 같다. 1. 백중밀, 금강밀, 우리밀 등 3품종의 0, 10, 30 및 50 μM ABA에서의 평균 발아지수와 발아율은 각각 0.95 S9, S10, S11, S12, S13, S14, S18)은 8개였고, 증가와감소가 동시에 이루어진 단백질 spot (S2)은 1개였다. 50 μM ABA에서만 검출된 단백질 spot (S7, S8)은 2개였다. 4. 각 단백질 spot의 0 μM ABA 처리구 양에 대한 50 μM ABA 처리구 양의 평균 배수 값(fold 값)이 1.5배 이상으로 증가한 단백질 spot은 S1 (globulin-3A), S6 (globulin-1 S allele), S16 (globulin-1 S allele), S17 (globulin-1 S allele) 등으로 모두 globulin류 단백질이었다. 또한 50 μM ABA에서만 확인된 단백질 spot인 S7 (globulin 3)과 S8 (globulin-1 S allele)도 globulin 단백질이었다. 5. 각 단백질 spot의 0 μM ABA 처리구 양에 대한 50 μM ABA 처리구 양의 평균 배수 값(fold 값)이 0.7 이하로 감소한 단백질 spot은 S10 (glutamine sysnthetase cytosolic isozyme), S12 (S-adenosylmethionine synthetase 2), S14 (isocitrate dehydrogenase NADP)이었다. 이상의 결과는 ABA에 의한 밀 유묘의 유아와 유근의 생장억제는 배아에서의 ABA 농도 증가, 그리고 이에 따른 배아의 glutamine 합성에 관여하는 다양한 효소의 발현 감소및 메틸기공여물질의 감소와 이에 따른 메틸기 전이활성의감소 등이 관여하고 있음을 의미한다. 한편 배아에서의 ABA 에 의한 globulin 단백질의 증가는 배아 특이적 globulin의일시적 합성 증가와 globulin 분해 효소의 활성 억제 등이복합적으로 관여한 결과로 생각된다.

경계조건 분석을 통한 LCCBS 연계방안

정재혁,김태희 한국건축시공학회 2013 한국건축시공학회지 Vol.13 No.4

Costs and expenses are intertwined and incurred throughout an entire construction project, even from the pre-construction phase, and each phase has a different impact on the life cycle cost (LCC). However, the cost breakdown structure (CBS) is different in each phase of a building construction project, which makes it hard to reasonably calculate construction cost. For this reason, the boundary conditions were analyzed in this study based on the life cycle cost break structure (LCCBS). In addition, breakdown factors were analyzed based on the boundary conditions to derive a linkage method. The validity of the linkage method was verified through application to actual construction projects. Through the analysis, it was found that the problem of items being left out was reduced by more than 97.2 percent, and the work was done an average of 6 hours faster compared to the conventional method. It is expected that by applying the new LCC system, LCC will be both reduced and calculated in a more efficient manner 건축물의 생애주기비용은 시행과정 전 단계에서 상호 연관되어 비용이 발생하며 각 단계마다 서로 간에 영향력이 존재한다. 하지만 건축 프로젝트의 각 단계별 CBS가 서로 상이하여 합리적인 공사비를 산정하는데 문제점이 발생한다. 따라서 본 연구에서는 기존에 연구되었던 LCCBS를바탕으로 건축 프로젝트 각 단계 간에 경계조건을 분석하였다. 또한 분석된 경계조건을 바탕으로 분절요인을 분석하고 이에 따른 연계방안을 모색하였다. 연계방안의 유효성 검증을 위해 실제 건축프로젝트를 바탕으로 사례적용을 실시하였다. 분석결과 누락되는 항목이 97.2%이상을개선하였고 평균 6시간 빠르게 업무를 수행할 수 있음을확인할 수 있었다. 향후, LCC 시스템에 적용함으로써LCC의 효율적인 산정 및 LCC 절감을 계획할 수 있을 것으로 사료다.

갯개미자리(Spergularia marina Griseb)의 염농도에 따른 발아 및 생장 반응

정재혁,김선,이장희,최원영,이경보,조광민 韓國作物學會 2014 한국작물학회지 Vol.59 No.2

본 연구는 한국 서남해안지역에 분포하고 있는 갯개미자리의 염농도에 따른 발아와 생장반응을 구명하고자 수행하였으며 결과는 아래와 같다. 1.갯개미자리의 염농도 및 온도별 발아율을 조사한 결과, NaCl 0~1.0%에서는 15℃에서 발아율이 90% 이상으로 높았고, NaCl 2.0%에서는 모든 온도에서 발아가 되지 않았다. NaCl 0%에서 발아율은 10℃에서 20℃까지는 90% 이상을 보였지만, 25℃에서는 51.7%로 급격히 낮아졌다. 또한 15℃에서 NaCl 농도별 발아율이 80% 이상 되는 시기는 NaCl 0%와 0.3%에서는 5일, NaCl 0.5%에서는 7일, NaCl 1%에서는 9일이었고, NaCl 2%에서는 발아하지 못하였다.2.염농도별로 8주 동안 양액재배한 결과, 염농도가 NaCl 0, 50, 100, 200, 300, 400 mM에서는 NaCl 50 mM 처리구가 초장 12.5 cm, 건물중 0.73 g로 생장이 가장 좋았다. 또한 해수 0, 12.5, 25, 50, 100%에서는 해수 12.5% 처리구가 초장 20.9 cm, 건물중 0.72 g으로 생장이 가장 좋았으며, 이들 염농도는 모두 NaCl 0.3% 수준이었다.3.염농도에 따른 무기성분 함량은 NaCl 농도가 높을수록 Na+ 함량은 높아졌지만, K+과 Ca+, Mg+은 낮아지는 경향을 보였다. 특히 Na+ 함량과 K+ 함량이 대조적으로 변화하였으며, 이들은 NaCl 50 mM에서 함량이 급격히 변화한 다음 NaCl 400 mM까지 완만한 함량 변화를 보였다. 4.이상의 결과로 볼 때, 갯개미자리는 안정적인 발아를 위해 NaCl 1.0% 이하에서 15℃ 유지가 필요하고 재배에서는 NaCl 0.3% 수준이 적정 염농도로 생각 된다. This study was conducted to investigate the germination and growth response by Salinities of Spergularia marina Griseb grown in the western coastal region in South Korea. The germination was investigated for 10 days at temperature 10℃, 15℃, 20℃, 25℃ in order to examine the germination of Spergularia marina Griseb by NaCl concentration 0.0, 0.3, 0.5, 1.0, 2.0%. The germination of NaCl concentration 0.0~1.0% was 90% over at 15℃ treatment, but the germination of NaCl concentration 2.0% was 0% at all temperature treatments. To identify the growth response of Spergularia marina Griseb according to the salinity, Spergularia marina Griseb was cultivated for 8 weeks in Hoagland culture medium and sea water. In nutrient solution culture, growth was best in NaCl 50mM among 0∼400 mM concentrations and in 0, 12.5, 25, 50, 100% of sea water, growth was best in 12.5% (dilution ratio with fresh water) treatment. Spergularia marina Griseb’s inorganic component contents according to salinity showed that Na+ content increased, but contents of K+, Ca+, Mg+ decreased. As a result, appropriate condition for Spergularia marina Griseb’s germination is considered to be maintained at 15℃ and in less than NaCl 1.0% of salinity. When nutrient solution culture, NaCl 0.3% of treatment level is considered to be the optimum salinity.

산성교회 만나학당 이야기 속으로

정재혁 장로회신학대학교 기독교교육연구원 2019 교육교회 Vol.480 No.-

염농도에 따른 갯질경(Limonium tetragonum) 종자의 발아와 식물체의 생장 및 항산화 활성

정재혁,황운하,안승현,정한용,이현석,백정선,최경진,이건휘,나지은,정남진,이승재,윤성중 한국자원식물학회 2017 한국자원식물학회지 Vol.30 No.4

Limonium tetragonum is a halophyte grown naturally in the coastal region in South Korea. This study was conducted to investigate the effects of salt concentrations on seed germination, seedling growth, and antioxidant capacity of L. tetragonum. Seeds were collected from naturally grown plants of L. tetragonum and those at full maturity were used in this experiment. All experiments were performed at 0%, 0.5%, 1.0%, or 2.0% of salt concentrations. Seed germination rate was highest as 86% at 20℃ and followed as higher in order of 25℃, 30℃ and 15℃. The germination rate was about 80% at 0% or 0.5% of salt concentration, but it was very low at the salt concentrations higher than 1%. Growth of L. tetragonum seedlings showed no difference in Hoagland solution containing NaCl in the range of 0% to 1.0% and seedlings survived at 2.0% of NaCl concentration. As the salt concentration increased, the content of Na+ in the shoot increased, but that of K+, Ca++, or Mg++ decreased. The antioxidant activity and the content of total polyphenol and total flavonoid in the shoot were similar at 0% and 0.5% of NaCl and were highest at 2.0% of NaCl concentration. In conclusion, performance of seed germination and plant growth of L. tetragonum was highest at 0% and 0.5% of NaCl concentration, and showed no difference in antioxidant activity, total polyphenol contents, and total flavonoid contents at the same salt concentrations. 본 연구는 염생식물인 갯질경의 염농도에 따른 종자 발아, 식물체의 생장 및 식물체 내 항산화 활성, 총 폴리페놀 함량, 총플라보노이드 함량을 조사하고자 수행하였다. 갯질경 종자는20℃에서 발아율이 86%로 가장 높았고, 이어서 25℃, 30℃, 1 5℃ 순서로 발아율이 높았다. 20℃에서 갯질경 종자의 발아율은 염농도 0%와 0.5%에서 80% 이상으로 높았지만, 염농도1.0% 이상에서는 발아율이 매우 낮았다. 갯질경 식물체의 생장은 염농도 0%에서 1.0%까지는 차이가 없었고, 염농도 2%에서는 염농도 0%와 비교하여 60% 수준의 건물중량을 보였다. 식물체 내 무기성분 함량은 염농도가 높을수록 Na+ 함량은 증가하였고, K+, Ca++, Mg++ 함량은 감소하였다. 항산화 활성 및 총 폴리페놀과 총 플라보노이드 함량은 염농도 0%와 0.5%에서 비슷하였고, 염농도 2%에서 가장 높은 경향을 보였다. 따라서 염농도0.5% 이하의 토양에서 갯질경을 재배하면 안정적인 발아와 생장이 가능하고, 항산화 활성 및 총 폴리페놀과 총 플라보노이드함량의 변화가 적기 때문에 균일한 품질의 항산화 관련 재료를얻는데 유용할 것으로 판단된다.

東西醫學協力의 科學的 根據

鄭在赫 慶熙大學校 東西醫學硏究所 1986 國際東西醫學硏究論文集 Vol.1986 No.-

The Scientific Foundation of Cooperation on East-West Medicines Ⅰ) The foundation of scientific theories of Oriental medicine : The philosophy of oriental medicine is a comprehensive, scientific theory. Contrary to the limits noted in experiments of modern sciences, philosophy does not restrict itself because of its allout view. Ⅱ) The philosophical contents of Oriental medicine : Oriental medicine, in contrast to modern medical science, founds its theory of medicine upon philosophy. This philosophy has for its authority the classics of the orient, among the most important of which include the Whang Ti Nei Ching, the I Ching (or the Book of Change), and the Chung Yung(the Doctrine of the Mean). Ⅲ) The Ethics of oriental Medicine : The Yin-Yang Theory which account for both the philosophy and science of Oriental medicine also deals with its ethics which are recorded in the I Ching and Chung Yung. It is an important fact that Oriental medicine included the subject of ethics, because the same degree of importance is placed upon the role of ethics in medicine as is on scientific knowledge or technique. Ⅳ) The clinical significances of Oriental Medicine : First, we will look into the character of preventive medicicine in Oriental medicine. Secondly, we come to the psychosomatic side of Oriental medicine. Thirdly, the pharmaceutics of Oriental medicine. The pharmaceutical therapy of Oriental medicine consists in medicinal decoctions, pills, and powder or raw medicine and so forth. Fourth is the effects of physical therapy. Acupuncture, moxibustion and many other physical therapies are mentioned in the Nei Ching, the source of the original text of Oriental medicine. Finally, its value as an informative medicine. Because Oriental medicine is empirical and built upon knowledge collected during the passing of time, valuable information can be found in there.

Expulsion of Fibroids to the Endometrial Cavity after Magnetic Resonance Imaging-guided High Intensity Focused Ultrasound Surgery (MRgFUS) Treatment of Intramural Uterine Fibroids

정재혁,홍길표,김유리,홍다교,하재은,염정인,김은정,김형일,이규섭 대한폐경학회 2016 대한폐경학회지 Vol.22 No.3

Objectives: This report seeks to introduce some cases of the patients who received magnetic resonance imaging (MRI)-guided high intensity focused ultrasound (HIFU) surgery (MRgFUS)-based intramural uterine fibroids treatment where the post-MRgFUS intramural uterine fibroids decreased in its volume and protruded towards the endometrial cavity to be expelled by hysteroscopy. Methods: Of the 157 patients who had received MRgFUS treatment in the Obstetrics and Gynecology of the Hospital from March, 2015 to February, 2016; this study examined 6 of the cases where, after high intensity focused ultrasound treatment, intramural uterine fibroids protruded towards the endometrial cavity to be removed by hysteroscopic myomectomy. The high intensity focused ultrasound utilized in the cases were Philips Achieva 1.5 Tesla MR (Philips Healthcare, Best, The Netherlands) and Sonalleve HIFU system.Results: The volume of fibroids ranged from 26.0 cm3 to 199.5 cm3, averaging 95.6 cm3. The major axis length ranged from 4.0 cm to 8.2 cm, averaging 6.3 cm. Fibroid location in all of the patients was in intramural uterine before treatment but after the high intensity focused ultrasound treatment, the fibroids were observed to protrude towards the endometrial cavity in at least Day 5 or up to Day 73 to allow hysteroscopic myomectomy.Conclusions: In some cases, after an intramural uterine fibroid is treated with MRgFUS,, fibroid volume is decreased and the fibroid protrudes towards the endometrial cavity. In this case, hysteroscopic myomectomy can be a useful solution. (J Menopausal Med 2016;22:139-145)

임베디드 데이터베이스 시스템을 위한 블록 단위 스키핑 기법

정재혁,박형민,홍석진,심규석,Chong, Jae-Hyok,Park, Hyoung-Min,Hong, Seok-Jin,Shim, Kyu-Seok 한국정보처리학회 2009 정보처리학회논문지D Vol.16 No.6

일반적으로 데이터베이스 시스템에서의 질의 수행은 대부분의 경우 빠른 응답시간과 더 적은 메모리 사용량을 장점으로 가지는 파이프라이닝 기법으로 이루어진다. 이 때, 질의 수행 계획(QEP)의 각각의 연산 노드들은 Open(), Next(), Close() 함수들을 지원하는 iterator의 인터페이스를 가진다. 그런데, 플래시 메모리 기반의 휴대용 기기들을 위한 임베디드 데이터베이스 시스템에서는 iterator의 Next() 함수뿐만 아니라, 현재 레코드의 이전 레코드를 리턴해주는 Previous()와 같은 함수를 필요로 하는 경우가 많다. 이는 임베디드 환경의 경우 각각의 프로그램이 사용할 수 있는 메모리의 양이 제한적이므로, 사용자가 이전 레코드를 요청하는 경우, 결과 레코드 커서가 현재 레코드를 기준으로 이전 레코드를 다시 가져와야 하기 때문이다. 본 논문에서는 이러한 임베디드 데이터베이스 시스템의 질의 수행 시 각각의 연산 노드들이 Next() 함수뿐만 아니라 Previous() 함수를 블록 단위로 지원할 수 있도록 새롭게 설계 구현하는 과정에서 발생하는 방향 전환 문제를 소개하고 이를 해결하기 위한 블록 단위 스키핑 기법을 제안한다. Today, most of all the query processors in the world generally use the 'Pipelining' method to acquire fast response time (first record latency) and less memory usage. Each of the operator nodes in the Query Execution Plan (QEP) provides Open(), Next(), and Close() functions for their interface to facilitate the iterator mechanism. However, the embedded database systems for the mobile devices, based on the FLASH memory, usually require a function like Previous(), which returns the previous records from current position. It is because that, in the embedded environment, the mobile devices cannot fully provide it main memory to store all the query results. So, whenever needed the previously read records the user (program) should re-fetch the previous records using the Previous() function: the BACKWARD data fetch. In this paper, I introduce the 'Direction Switching Problem' caused by the Previous() function and suggest 'Block-wise Skipping' method to fully utilize the benefits of the block-based data transfer mechanism, which is widely accepted by most of the today's relational database management systems.

IoT 응용을 지원하기 위한 뉴로모픽 하드웨어 기반 지능형 컴포넌트 설계

정재혁,김서연,정진만,윤영선 한국정보과학회 2021 한국정보과학회 학술발표논문집 Vol.2021 No.6

Deformation Behavior of TRIP Steel Studied by In-situ Synchrotron X-ray Diffraction and EBSD (초)

정재혁,김한수,( Zhengang Xu ),( Soran Birosca ),장창환,( B. C. De Cooman ) 대한금속재료학회 ( 구 대한금속학회 ) 2007 대한금속재료학회 학술대회 개요집 Vol.2007 No.1