국가전략소재로서의 희유금속

이수영,지율권,최한신,김용환,이진규,김택수,Lee, Soo-Young,Ji, Yul-Kwon,Choi, Han-Shin,Kim, Hwan-Yong,Lee, Jin-Kyu,Kim, Taek-Soo 한국분말야금학회 2009 한국분말재료학회지 (KPMI) Vol.16 No.3

간(肝) 지방(脂肪) 침윤(浸潤)에 대한 초음파(超音波) 소견(所見)과 혈액(血液) 생화학적(生化學的) 검사(檢査)의 상관관계(相關關係)에 관한 연구(硏究)

이수영,문수형,이화숙,김경희,김강석,Lee, Soo-Young,Moon, Soo-Hyung,Lee, Hoa-Suk,Kim, Kyeong-Hee,Kim, Kang-Seuck 한국생명보험의학회 1985 保險醫學會誌 Vol.2 No.1

In medical selection of Daehan Education Insurance, We doubted that 140 cases(male 117 cases, female 23 cases) might have the fatty infiltration of the liver by ultrasonography. We examined the weight and the hypertension of 140 patients by biochemically analyzing the blood extracted under fasting according to the age-distribution. The results are as follows: 1. 90% out of them had body weight than normal subject. 34.29% out of them were hypertension patients and most of them were $30{\sim}39's$ and $40{\sim}49's$. 2. As compared with the normal subjects in serum lipid value, HDL cholesterol value was low, but total cholesterol, Triglyceride, phospholipid and total lipid value were significantly high. Especially the statistical value of Triglyceride was very significantly high. 3. Total abnormal rate of sGOT value in Liver function test is 34.43% and the mean value of it is 66 unit and the abnormal rate of sGPT value is all 3.14% and the value of it is 70 unit. 4. The abnormal rate of Alkaline phosphatase value is 9.29% and the abnormal rate of total Bilirubine value is 14.29%. As HBsAg positive rate is 2.14% and HBs Ab positive rate is 31.43%, the positive rate of HBsAg represents Lower positive rate than Korean mean value. 5. The abnormal rate of the fasting blood glucose is 15.00% and the abnormal rate of Blood sugar pc 2hrs is 30.71%. 6. The abnormal case of protein, Albumin, Globulin, BUN, craetinine and Hemoglobin value was not found to any of them and in the above liver function test except serum lipid value, the rate not showing the abnormal value represents 45.3%(male) and 52.17%(female).

Expression of an artificial gene encoding a repeated tripeptide lysyl-g1utamyl-tryptophan in Tobacco Plant

이수영,나경수,백형석,박희성,조훈식,이용세,최장원,Lee, Soo-Young,Ra, Kyung-Soo,Baik, Hyung-Suk,Park, Hee-Sung,Cho, Hoon-Sik,Lee, Young-Se,Choi, Jang-Won Korean Society of Life Science 2002 생명과학회지 Vol.12 No.1

식물 단백질의 영양가 향상을 위한 일환으로 필수아미노산의 조성이 풍부한 인공단백질을 암호화하는 인공유전자를 담배 식물체에서 발현을 시도하기 위하여, 식물에서 외래유전자의 발현에 널리 사용되는 Cauliflower mosaic virus (CaMV)의 35S promoter를 이중으로 중첩되도록 하고, (Lys-Glu-Trp)이 64번 반복되는 인공유전자 및 nopaline synthase (nos) terminator를 갖고있는 binary vector pART4-4를 구성하였다. 이 재조합 플라스미드는 Agrobacterium tumefaciens를 이용한 형질전환에 의해 Nicotiann tabacum (Var. Xanthi)으로 도입되었다. Kanamycin이 포함된 신초 유도 배지 및 뿌리 유도배지를 이용하여 정상적으로 재생된 담배 식물체로부터 도입된 인공유전자의 발현을 분석하였다. 추출한 genomic DNA를 EcoRI으로 자른 다음 Southern blot 분석에 의하면, 효소 절단 시 예상되는 1.1 kb에서 band를 형성하였으며 각각의 형질전환 식물체에 인공유전자가 1 또는 3 개씩 도입되어 있음을 확인하였다. Northern blot 분석에 의하면 약 1.2 kb 전사체가 비교적 안정하게 발현되었으며, 잎, 줄기, 뿌리로부터 RNA를 분리하여 promoter의 조직 특이성 발현을 분석한 결과, 잎에서 생성되는 RNA가 줄기나 뿌리 조직보다 안정하게 발현되었다. 형질전환 식물체에서 Western blot에 의한 단백질 분석 결과, 잎에서 추출한 단백질로부터 원하는 크기인 33 kDa의 인공단백질이 생성됨을 확인하였으며 발현 수준은 전체 세포 단백질의 0.1%로서 낮은 수준이었다. To investigate expression of the artificial gene encoding a repeated tripeptide lysyl-glutamyl-tryptophan in tobacco plant, the plant binary vector, pART404 has been constructed, which contains the duplicated CaMV 35S promoter, an artificial gene coding for repetitive polymer (Lys-Glu-Trp)$_{64}$, and nopaline synthase (nos) terminator. The recombinant expression vector was introduced in Nicotiana tabacum (var. Xanthi) via Agrobacterium tumefaciens-mediated trans-formation. The transgenic calli selected by kanamycin containing medium were then regenerated to whole plants. Southern blot analysis indicated that five transgenic plants (No. 1, 7, 9, 43, 45) showed the hybridizing signals at 1.1 kb of the expected size on EcoRI digestion and each of the transgenic plants contained 1 or 3 copies of the artificial gene inserted into its genome. By northern blot analysis, the size of the hybridized total RNA was estimated to be approximately 1.2 kb and the RNA appeared generally to have the integrity. Western blot indicated that the protein was detected at the position of 33 kDa and the expression level of the polypeptide in the transgenic plant (No. 45) was measured to approximately 0.1% of the total protein.

혈장 세로토닌과 외상후 스트레스 장애 : 월남전 참전 재향군인을 대상으로

이수영,강석훈,정문용,이명희,김태용,소형석,정혜경,최진희,Lee, Soo-Young,Kang, Suk-Hoon,Chung, Moon-Yong,Lee, Myung-Hee,Kim, Tae-Young,So, Hyung-Seok,Chung, Hae-Kyung,Choi, Jin-Hee 대한불안의학회 2009 대한불안의학회지 Vol.5 No.1

Objective : The objective of this study was to examine the relationship between plasma serotonin concentration and posttraumatic stress disorder (PTSD) symptoms in chronic PTSD patients who have been taking medication. Methods : Plasma serotonin level of 14 PTSD patients and a control group of 28 Vietnam War veterans was measured by HPLC (high performance liquid chromatography). The Combat Exposure Scale (CES), Mississippi Scale for Combat-Related Posttraumatic Stress Disorder (M-PTSD), Clinician Administered PTSD Scale (CAPS), Hamilton Rating Scale for Depression (HRSD), and Hamiltion Anxiety Scale (HAS) were used to evaluate PTSD symptom severity. Results : Serotonin level was significantly higher in the PTSD group than in the control group (p=0.036, p=0.006, respectively). M-PTSD (p<0.001), CAPS (p<0.001), HRSD (p<0.001), and HAS (p<0.001) scale scores were significantly higher in the PTSD group than in the control group; however, the CES score failed to show a significant improvement (p=0.964). There were no significant differences between plasma serotonin and PTSD symptoms. Conclusion : In chronic PTSD patients who have been taking medications, we can not predict treatment effect and symptom severity by measuring only plasma serotonin levels. PTSD is a complicated disorder which may likely be related to a variety of neurotransmitter systems. Therefore, further research which investigate relationships with norepinephrine, dopamine, and other neurotransmitters as well as serotonin is needed to improve the treatment of PTSD.

국내 11-12세 소아에서 Td 백신 추가접종의 면역원성과 안전성 평가

이수영,곽가영,목혜린,김종현,허재균,이경일,박준수,마상혁,김황민,강진한,Lee, Soo Young,Kwak, Ga Young,Mok, Hye Rin,Kim, Jong Hyun,Hur, Jae Kyun,Lee, Kyung Il,Park, Joon Su,Ma, Sang Hyuk,Kim, Hwang Min,Kang, Jin Han 대한소아청소년과학회 2008 Clinical and Experimental Pediatrics (CEP) Vol.51 No.11

Purpose : This study was undertaken to evaluate the immunogenicity and reactogenicity of Td booster immunization in early preadolescents of Korea. Methods : Healthy preadolescents, who had been vaccinated with 4 or 5 doses of DTaP vaccines until 6 years old age, were enrolled in this study from August 2006 to April 2007. Diphtheria and tetanus anti-toxoid antibodies in sera were measured by ELISA just before vaccination and 4 weeks after vaccination to evaluate immunogenicity. Local and systemic adverse reactions observed for 4 weeks after vaccination to access reactogenicity. Results : 183 preadolescents were enrolled and mean age was $11.40{\pm}0.51$ years old. All subjects achieved seroprotective diphtheria and tetanus anti-toxoid antibodies (titers ${\geq}0.1IU/mL$) after Td booster vaccination. Among 183 vaccinees, 73.8% showed local adverse reactions and 37.2% systemic adverse reactions. Pain at injection site (66.1%) was the most common local reaction, and the most commonly shown systemic reaction was myalgia (17.5%). The adverse reactions were spontaneously relieved within three days after vaccination. Conclusion : Td vaccine in this study was high immunogenic and showed an acceptable tolerance in Korean preadolescents. Td booster vaccination at 11-12 years old is the most effective method to increase compliance of the vaccination and to decrease the incidence of diphtheria and tetanus. 목 적: 11-12세 연령에 Td 백신 1차 추가접종을 하는 방법에 대한 면역원성과 안전성을 평가하기 위해 연구를 계획하였다. 방 법: 2006년 8월부터 2007년 4월까지 연구병원 소아청소년과 외래에 Td 백신 접종을 받기 위해 내원한 11-12세의 소아를 대상으로 하였다. 면역원성을 평가하기 위하여 접종 전 및 접종 4주 후에 혈액을 채취하여 디프테리아 및 파상풍에 대한 항독소 항체가를 측정하였고 이상반응을 평가하기 위해 관찰 일지에 국소 및 전신 이상반응을 기록하였다. 결 과: 총 183명이 연구에 참여하였고 이들의 평균 연령은 $11.40{\pm}0.51$세이었다. Td 백신 접종 전후의 GMC는 디프테리아에 대해서는 10배, 파상풍에 대해서는 26배 이상 증가하였고, 접종 후 디프테리아와 파상풍에 대한 항체 양전율(항체가 ${\geq}0.1IU/mL$ 기준)은 100%이었다. 디프테리아의 접종 전 항체가가 0.1 IU/mL 이상인 피험자는 142명(77.6%)이었고 접종 후 항체가가 1.0 IU/mL 이상인 피험자는 174명(95.1%)이었다. 파상풍의 접종 전 항체가가 0.1 IU/mL 이상인 피험자는 146명(79.8%)이었고 접종 후 항체가가 1.0 IU/mL 이상인 피험자는 181명(98.9%)이었다. 접종 후 국소 이상반응이 73.8%, 전신 이상반응은 37.2%에서 발생하였으나 대부분 3일 이내 소실되었다. 결 론: 매우 높은 면역원성과 심하지 않은 이상반응을 고려할 때, Td 백신의 접종을 11-12세 시행하는 것은 디프테리아와 파상풍에 대한 가장 경제적인 방어 수단이며, 접종 순응도를 효율적으로 높일 수 있는 방법이다.

Haemophilus influenzae Type b 에 대한 급성 후두염의 치료 경험 2 례

이수영(Soo Young Lee),유상호(Sang Ho Yoo),이기선(Ki Sun Lee),홍미애(Mi Ae Hong),김순남(Soon Nam Kim) 대한소아알레르기호흡기학회 1998 소아알레르기 및 호흡기학회지 Vol.8 No.1

Epiglottitis is an uncommon but potentially life threatening infectious disease in young children. And it is rapidly progressing cellulitis of the epiglottis and adjacent structures that has the potential for causing abrupt, complete airway obstruction. The most common cause of acute epiglottitis is Haemophilius influenzae type b (Hib), therefore in USA, the acute epiglottitis is seen less commonly since the wide spread use of immunization against Hib. In Korea, there has been no report of acute epiglottitis caused by known bacterial organism, and a little investigation of the effects of the immunization against Hib, or Hib related respiratory diseases. In this report, we describe two cases of acute epiglottitis caused by Hib, occurred in non-immunized young-male children admitted to Ajou University Hospital. They had visited to the emergency center of Ajou University hospital with the complaints of acute fever, drooling, dysphagia and severe respiratory difficulties. In both cases, we observed the cherry-red colored, severely swollen epiglottis by the direct laryngoscopic examination. Hib was cultured in blood samples from both two cases, and the patients treated successfully by the 3 days of endotracheal intubation and proper antibiotics therapy without any complications such as pneumonia, meningitis, osteomyelitis, or pericarditis.

백일해에 대한 연령별 면역혈청학적 연구

이수영,최의윤,김주상,안중현,최정현,마상혁,박준수,김황민,강진한,Lee, Soo Young,Choi, Ui Yoon,Kim, Ju Sang,Ahn, Joong Hyun,Choi, Jung Hyun,Ma, Sang Hyuk,Park, Joon Soo,Kim, Hwang Min,Kang, Jin Han 대한소아감염학회 2012 Pediatric Infection and Vaccine Vol.19 No.2

목적: 우리나라의 백일해에 대한 연령별 방어면역 상태를 평가하고자 면역혈청학적 연구를 시행하였다. 방법: 국내 4개 대학병원에 방문한 건강한 소아, 청소년 및 성인을 대상으로 하였다. 대상을 연령에 따라 7개 연령군으로 구분하였고 ELISA 방법을 이용하여 백일해의 항체가를 측정하였다. 연령군별 기하 평균항체가와 각 연령군별 양성 항체가를 보이는 피험자의 비율을 조사하였다. 제조사의 지침대로, 항체가가 24.0 EU/mL 이상인 경우를 양성으로 판정하였다. 결과: 전체 1,605명의 피험자가 연구에 참여하였고, 기하 평균항체가는 $56.16{\pm}50.54EU/mL$였다. 연령군별로는 11세 미만 소아 연령군($64.78{\pm}53.24EU/mL$)이 가장 높은 기하 평균항체가를 보였다(P<0.001). 양성 항체가를 보인 피험자의 비율은 전체 대상의 68.2%였고, 11세 미만 소아 연령군(76.5%)이 가장 높은 비율을 보였다(P<0.001). 결론: 소아 연령군과 비교하여, 청소년과 성인 연령군에서 백일해에 대한 낮은 기하 평균항체가를 나타냈으며 양성 항체가를 갖는 피험자의 비율이 낮다는 것을 확인하였다. Purpose: We conducted the immunoassay of pertussis according to ages, in order to evaluate protective immunity against pertussis in Korean populations. Methods: Healthy subjects were enrolled at four university hospitals in Korea. The subjects were grouped as seven age groups (every 10 years). Antibodies against pertussis toxin (PT) in sera were measured by enzyme linked immunosorbent assay (ELISA) kits. Geometric mean concentrations (GMC) of antibodies and the ratios of the subjects with seroprotective antibody levels were determined. The subjects with antibody titers ${\geq}24.0EU/mL$ were considered to seroprotective as the manufacturer's protocol. Results: Total 1,605 subjects (age: 2 months-65 years) participated in this study, and their GMC was $56.16{\pm}50.54EU/mL$. Among seven age groups, age group <11 year showed the highest GMC ($64.78{\pm}53.24EU/mL$) (P<0.001). In the analysis of the ratios of the subjects with seroprotective antibody titers, 68.2% of the subjects were proven to seroprotective, and age group <11 year also showed the highest ratio (76.5%) (P<0.001). Conclusions: We found that adolescences or adults (age group ${\geq}11$ year) showed lower levels of antibody against pertussis and lower ratio of the subjects with seroprotective antibody titers than children (age group <11 year).

구강스캐너와 급속 지르코니아 소결을 이용한 당일 풀지르코니아 보철수복

이수영,Lee, Soo Young 대한심미치과학회 2016 Journal of the Korean Academy of Esthetic Dentistr Vol.25 No.2

Single visit monolithic restoration can be proceed with digital workflow which consist of intraoral scanning, dental CAD(computer aided design) and restoration milling with CAM(Computer aided manufacturing). While zirconia has more than 900MPa of flexural strength compared with 400MPa for lithium disilicate, shortened fabricating time of lithium disilicate is considered to be a better choice for fabricating single visit full contour monolithic restoration. However, new zirconia materials which are TZI C(Dentsply Sirona) and LUXEN Enamel(Dental Max), new induction heating method of sintering furnace, and new sintering protocols for MoSi2 heating elements sintering furnace offer significantly reduction of full contour monolithic zirconia restoration fabrication time with greater translucency. These new developments lead single visit zirconia restoration in reality. 당일수복 보철물 제작을 위해 디지털 프로세스 사용은 필수적이다. 이러한 디지털 프로세스는 구강스캔, 컴퓨터를 이용한 보철물을 디자인 과정(CAD)과 보철물을 가공하는 과정(CAM)을 포함한다. 지르코니아의 굴곡강도는 900Mpa로 리튬디실리케이트의 400MPa보다 더 단단하지만, 리튬디실리케이트를 이용한 보철물 기공시간이 지르코니아와 비교하여 더 짧기에 당일수복 보철물 제작시 리튬디실리케이트 사용이 선호되었다. 하지만, TZI C 와 LUXEN Enamel 와 같은 새로운 소재의 지르코니아가 출시되고, 새로운 소결방식이 개발되어 지르코니아 기공시간을 단축시킬수 있게 되었고, 높은 투명도의 풀지르코니아 크라운을 짧은 시간 안에 제작할 수 있게 되었다. 이러한 발전은 당일 풀지르코니아 보철수복을 현실로 이끌고 있다.

생명보험(生命保險) 계약자(契約者)에서 B형간염(型肝炎) 표면항원(表面抗原)과 표면항체(表面抗體) 양성율(陽性率) 조사(調査) 연구(硏究) : vaccine접종(接種) 이후(以後)의 변화(變化) 양상(樣相)

이수영,이화숙,김경희,김강석,Lee, Soo-Young,Lee, Hoa-Suk,Kim, Kyeong-Hee,Kim, Kang-Seuck 한국생명보험의학회 1986 保險醫學會誌 Vol.3 No.1

To investigate the Positive rate of anti HBs and HBs Ag positive rate of the each group of vaccinated hepatitis B type and not-vaccinated, male 4150 female 2475, from May 1, 1984 to November 30, 1985 in the Medical department of Dae han Kyoyuk ins. Co., We examined into the HBsAg and Anti HBs by using the RPHA method and PHA method and the results which were adjusted statistically were as follows. 1. HBs Ag positive rate was 9.0%(228/2531)in 1984 and 7.0%(287/4069) in 1985, the positive rate in 1985 represented 2% lower than in 1984. 2. Anti HBs positive rate was 43.3%(1096/2531) in 1984 and 42.8%(1744/4069) in 1985, there was no significant change for two years. 3. Anti HBs positive rate in the group of the not-vaccinated hepatitis B type was 39.2%(869/2215) in 1984 and 38.8%(1333/3432)in 1985. 4. Anti HBs positive rate in the group of the vaccinated hepatitis B type more than once was 64.7%(419/647)in male and 71.5%(219/306) in female that was 66.9%(638/953) in total. 5. Anti HBs positive rate in the group of the vaccinated hepatitis B type three times was 68.7%(270/393) in male and 80.0%(156/195) in female and 72.4% in total. In this conclusion; The formation of Anti HBs in female showed more or less higher than male. 6. The cases which were detected HBs Ag and anti HBs at the same time were 4 in male and 3 in female and in the group of the vaccinated hepatitis B type, the number of HBs Ag positive cases were 8 in male and 10 in female. (On condition that we didn't distinguish $5{\mu}g/ml$, hepatitis B type vaccine, from $20{\mu}g/ml$ and after vaccinating, the lapsed time was not settled.)

혈장 Plasminogen 과 Prothrombin 의 생산에 관한 연구

이수영,Lee, Soo-Young 생화학분자생물학회 1979 한국생화학회지 Vol.12 No.1

토끼의 정맥에 주사된 [$^{75}Se$]-L-methionine에 편입된 혈장 plasminogen과 prothrombin의 편입율은 주사후 30분 부터 출현하기 시작하여 3시간 후에 최고치에 도달한 뒤 plateau를 이루었다. Plasminogen과 prothrombin은 다 같이 혈장에 존재한다는 것은 잘 알려진 사실이다. (Biggs & Macfarlane, 1962). plasminogen은 섬유소(fibrin)를 용해시키기 위해서 plasminogen activator에 의해서 활성 형인 plasmin으로 전환된 다음에 섬유소에 작용한다는 것도 이미 다 알려져 있지만 plasmin의 체내 생산장소에 대해서는 아직까지 확실히 알려져 있지 않고 다만 면역형광법에 의한 관찰 결과, 골수의 호산성과립구(eosinophilic leukocyte)에서 생산되어 순환내로 유리되는 것이 아닌가 추측되고 있다(Barnhart & Riddle, 1963). 한편 prothrombin은 혈액이 응고될 때에 prothrombinase에 의하여 활성화 되어 thrombin으로 되며, 이 thrombin이 칼슘이온과 함께 섬유소원(fibrinogen)에 작용하여 섬유소(fibrin)로 전환시키며, 또 이 prothrombin의 생산장소는 간이라는 것은 잘 알려진 사실이다(Finkbiner et al., 1959; Rapaport et al., 1960). 저자는 혈액응고 과정에서 섬유소원을 섬유소로 전환시키는데 작용하는 thrombin의 zymogen형인 prothrombin과 섬유소를 용해시키는데 작용하는 plasmin의 zymogen형인 plasminogen이 체내의 생산장소로부터 각각 생산된 뒤에 순환혈액으로 유리되는 방식(mode)을 관찰하기 위하여, 추적자로서 [$^{75}Se$]-L-methionine을 토끼의 정맥에 주사한 뒤에, 일정한 시간 간격으로 도살하고, 혈장 plasminogen과 thrombin에 각각 편입된 [$^{75}Se$]-L-methionine의 방사능을 계측하였다. Both plasminogen and prothrombin incorporated by [$^{75}Se$]>-L-methionine in the plasma of rabbits were rapidly increased up to 3 hours after [$^{75}Se$]-L-methionine infusion, and then showed plateaus, respectively.