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$17{\alpha}-methyltestosterone$에 의한 능성어 Epinephelus septemfasciatus의 성전환 유도
송영보,백혜자,김형배,이경준,이명돈,Song, Young-Bo,Baek, Hae-Ja,Kim, Hyung-Bae,Lee, Kyeong-Jun,Soyano, Kiyoshi,Lee, Young-Don 한국양식학회 2005 韓國養殖學會誌 Vol.18 No.3
능성어 정자의 안정적 확보를 위하여 MT $0.5{\sim}2.0mg/kg$ BW를 어체 근육내 삽입하여 성전환을 유도하였다 사용한 실험어는 전장 $41.0{\pm}1.3cm$, 체중 $1.4{\pm}0.1kg$이었다. 실험 시작시 능성어 생식소는 주로 gonia cells과 주변인기 단계 난모세포를 가지고 있었다. 실험 종료시 대조구의 생식소는 실험 시작시와 유사하였다. 0.5 mg MT/kg BW 처리구 생식소는 실험 종료 때 소엽내강에 정원세포와 정세포 그리고 정자무리들이 대부분 차지하고 기부에 수정관이 형성되었으나, 일부 어린 난모세포들이 산재하였다. 1.0과 2.0 mg MT/kg BW처리구는 소엽내강과 기부의 수정관에 정자무리들로 가득 차 있었다. 정자는 $1.0{\sim}2.0mg$ MT/kg BW 처리구에서 얻을 수 있었다. MT 처리구의 T혈중농도는 대조구보다 2주 후부터 6주 후까지 높았으나(P<0.05), 배정이 가능한 8주째 대조구와 유사하였다(P>0.05). 11-KT 혈중농도는 MT 1.0처리구는 2주 후부터 6주째까지, 2.0 mg MT/kg BW 처리구는 2주 후부터 실험 종료 때까지 대조구 보다 높았다(P<0.05). $E_2$는 모든 실험어 일부에서 검출되었다. Sex reversal to a functional male of sevenband grouper $(41.0{\pm}1.3cm\;TL,\;1.4{\pm}0.1kg\;BW)$ was induced by $17{\alpha}-methyltestosterone\;(MT,\;0.5\sim2.0mg/kg\;BW)$ implantation from March 17 to May 12,2002. Gonad of control group was composed of genial cells and peri-nucleolus oocyte during the experimental period. Gonad of fish treated with 0.5 mg MT/kg BW had peri-nucleolus oocytes, spermatogonia, spermatids and spermatozoa at the late stages of spermatogenesis, while the fish group treated with 1.0 and 2.0 mg MT/kg BW contained spermatoza in the efferent duct. Sperm were obtained from the experimental groups treated with a dose of $1.0{\sim}2.0mg$ MT/kg BW. In the MT treated groups, testosterone and 11-ketotestosterone levels were higher than those in the control group during the $2{\sim}6$ weeks of the experimental period (P<0.05). $Estradiol-17{\beta}$ was detected from fish in the experimental fish.
송영보,이치훈,Hyeong-Cheol Kang,김형배,이영돈 한국발생생물학회 2013 발생과 생식 Vol.17 No.4
The fertilized eggs of E. septemfasciatus are spherical and transparent with buoyancy at 790 to 890 μm (average 821.8±2.0 μm) in diameter with 170 to 230 μm oil globules (average 192.9±0.93 μm). Hatching began approximately 46 and 35 hours after fertilization at 22.0℃ and 25.0℃ water temperature, respectively. The average total length of newly hatched larvae was 1.75±0.03 mm. Most of the yolk and oil globules were absorbed within 3 to 4 days after hatching. The larvae reached 2.48 to 2.72 mm in total length, and their mouths and anuses opened at 3 to 4 days after hatching. In this time, the mouth diameters of the larvae were 0.209 to 0.238 mm. The larvae reached 3.24 to 4.15 mm in total length at 11 to 17 days after hatching, and began to metamorphose at the time the second dorsal and pelvic spines appeared and elongated. The abdominal cavity was densely lined with melanophores. The larvae reached 5.12 mm in total length at 24 days after hatching.
Aralia cortex와 Phellodendron cortex의 혼합 추출물이 치주조직세포 활성에 미치는 영향
송영보,이만섭,권영혁,박준봉,허익,김성진,Song, Young-Bo,Lee, Man-Sup,Kwon, Young-Hyuk,Park, Jun-Bong,Herr, Yeek,Kim, Sung-Jin 대한치주과학회 1999 Journal of Periodontal & Implant Science Vol.29 No.1
The purpose of this study was to evaluate the effect of mixed extracts of aralia cortex and phellodendron cortex (P55A) on activities of human gingival fibroblasts and periodontal ligament cells in vitro. First experiment was done to evaluate the effect of P55A in normal condition. In control group, the cells($4.5{\times}10^4$ cells/ml) were cultured with Dulbecco's Modified Eagle's Medium contained with 10% fetal bovine serum. In experimental groups, P55A was added to the above culture condition at the final concentrations of 0.1 ${\mu}g/ml$(Test group 1), 1 ${\mu}g/ml$(Test group 2) and 10 ${\mu}g/ml$(Test group 3). Then each group was tested for the cell proliferation rate at $\frac{1}{2}$, 2, 5 days, protein levels at 2, 5 days, and alkaline phosphatase activity at 2, 5 days. Second experiment was done to evaluate the effect of P55A in high glucose condition. 200 mg/dl glucose was added to the same culture condition of all groups in first experiment. Then each group was tested for the cell proliferation rate at $\frac{1}{2}$ , 2, 5 days, protein levels at 2, 5 days, and alkaline phoaphatase activity at 2, 5 days. The results were as follows ; 1. First experiment 1) As P55A concentration increased, cell proliferation rate increased significantly in test group 2 at 2 days, and test group 2 and 3 at 5 days in human gingival fibroblasts and periodontal ligament cells(P<0.05). 2) In human gingival fibroblasts, all test groups showed significantly increased protein levels as compared to control group at 5 days. In periodontal ligament cells, test group 2 and 3 showed significantly increased protein levels as compared to control group at 2, 5 days(P<0.05). 3) Alkaline phosphatase activity of human periodontal ligament cells increased as P55A concentration increased. The test group 2 and 3 showed significant increase as compared to control group at 5 days(P<0.05). 2. Second experiment 1) As P55A concentration increased, cell proliferation rate increased significantly in test group 2 at 2 days, and test group 2 and 3 at 5 days in human gingival fibroblasts and periodontal ligament cells(P<0.05). 2) In human gingival fibroblasts, test group 3 showed significantly increased protein levels as compared to control group at 2 days, and all test groups at 5 days. In periodontal ligament cells, test group 2 and 3 showed significantly increased protein levels as compared to control group at 2, 5 days(P<0.05). 3) Alkaline phosphatase activity of human periodontal ligament cells increased as P55A concentration increased. The test group 2 and 3 showed significant increase as compared to control group at 2 days, and all test groups at 5 days(P<0.05). From the above results, mixed extracts of aralia cortex and phellodendron cortex appeared to enhance cellular activities including cell proliferation rate, protein levels and alkaline phosphatase activity of human gingival fibroblasts and periodontal ligament cells in normal and high glucose condition. This study suggests that mixed extracts of aralia cortex and phellodendron cortex seem to be able to subside the inflammation of periodontal tissue and regenerate the destructed periodontal tissue.
송영보,박용주,김한준,최면식,최영찬,이영돈 한국발생생물학회 2003 발생과 생식 Vol.7 No.1
2000년 2월부터 2001년 2월까지 남태평양 미크로네시아 군도의 Chuju 연안에 서식하는 주Epinephelus merra 암컷을 대상으로 생식주기를 조사하였다. 생식소숙도지수 (GSI)는 2월부터 증가하기 시작하여 3월에 최고치 (3.410.84)를 보였다. 조직학적 관찰결과 3월과 4월의 난소 안에는 난황을 가진 다양한 단계의 난모세포와 배란여포들이 존재하였다. 6월부터 1월에 난소 안에는 미성숙 난모세포만 있었다. 이들 결과로 E. merra The seasonal reproductive cycle of the female honeycomb grouper, Epinephelus merra, inhabiting Chuuk was examined by histological observations of the ovaries. The gonadosomatic index (GSI) began to increase in February and peaked in March. Histological observations revealed many oocytes laden with yolk in the ovaries from March to April. From June to January, the ovaries were occupied by immature oocytes. These results suggest that the reproductive season of E. merra in Chuuk is from March through April.
송영보,이치훈,나오수,이영돈 濟州大學校 海洋硏究所 2002 해양과환경연구소 연구논문집 Vol.26 No.-
1995년 9월부터 1996년 8월까지 제주대학교 해양과 환경연구소가 위치한 함덕연안 조간대에서 채집한 울타리고둥, M. labio의 생식주기를 조사하기 위하여 생식소숙도지수의 변화와 생식세포 형성과정을 조직학적방법으로 조사하였다. 1. 울타리고둥의 생식소는 패각내 나선상 육질부 하단에서 꼬리돌기까지 간장부의 표면에 위치 하였다. 생식소가 성숙하면 암컷은 짙은 녹색, 수컷은 유백색을 띠고, 방출 후에는 암컷은 연갈색, 수컷은 연황색을 나타내었다. 2. 생식소숙도지수(GSI)는 암컷과 수컷 모두 수온이 상승하는 4월부터 상승하기 시작하여 9월에 암컷 0.47, 수컷 0.42로 최고치를 보이고 이후 10월부터 급격히 감소하였다. 저수온기인 1월에 GSI는 암컷과 수컷 모두 0.07로 최저치를 보였다. 3. 생식소의 발달 단계는 분열증식기(3월∼4월), 성장기(4월∼7월), 성숙기(8월∼9월) 그리고 방출 및 회복기(10월∼1월)의 연속적인 주기로 구분 할 수 있고, 주산란 시기는 10월로 추정 된다. 4. 울타리고둥, M. labio은 자웅이체로서, 성비는 약 1:1이었다(P>0.05). Reproductive cycle of thick liped monodont. Mondonta labio was invetigated by the histological observation of gonads and the gonadosomatic index(GSI). The thick liped monodont were collected at the intertidal zone of Hamdeok in Jeju-do from September, 1995 to August, 1996. Gonad of them was located o the surface of the liver below the stomachal caecum posterior spiral meat part of the shell. GSI value began to increase from May(as water temperature increased) and reached it's maximum value in September both male and female which were 0.42 and 0.47, respectively. It began to decrease from October thereafter, maintaining relatively in low value from January to March. The reproductive cycle of this species could be classified into four successive developmental stage; multiplication stage(March to April), growing stage(April to July), mature stage(August to september), spent and recovery stage(October to January). The main spawning period of M. labio appeared in October. The top shell, M. labio appeared to be gonochorism, neither sex reversal nor hermaphroditism. The sex ratio was approximately 1.0 : 1.0(P>0.05).