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쥐 뇌에서의 Aldehyde Dehydrogenase의 분포에 관한 연구
이영돈,주충노,Lee, Young-Don,Joo, Chung-No 생화학분자생물학회 1993 한국생화학회지 Vol.26 No.8
쥐 뇌에서의 aldehyde dehydrogenase(ALDH) 활성분포를 조사하기 위하여 합성된 생체 aldehyde를 비롯한 여러 기질을 사용하여 부위에 따른 활성, 세포내 분포 및 isozyme의 존재 양상을 조사하였다. 뇌의 모든 부위에서 indole-3-acetaldehyde에 대한 총 ALDH활성은 acetaldehyde나 propionaldehyde에 대한 활성보다 높았다. $V_{max}/K_{m}$비를 통하여 기질과의 친화도를 조사한 결과 indole-3-acetaldehyde와 propionaldehyde에 대한 친화도는 뇌의 모든 부위에서 low-$K_m$ ALDH가 $high-K_m$, ALDH보다 훨씬 컸다. 특히 선조체의 ALDH는 다른 부위의 ALDH에 비하여 방향족 aldehyde에 대하여 낮은 $K_m$ 값과 높은 $V_{max}/K_{m}$비를 나타냈으며 3,4-dihydroxyphe-nylacetaldehyde(DOPAL)에 대하여 높은 활성을 나타내었다. 이같은 결과는 뇌조직에서 신경전달물질로부터 유래한 방향족 aldehyde는 주로 $low-K_m$ ALDH에 의해 산화되며 이 과정은 특히 선조체에서 dopamine의 산화과정에 크게 관여할 것으로 생각된다. Indole-3-acetaldehyde에 대한 ALDH활성의 세포내 분포의 경우, 미토콘트리아분획의 활성은 시상하부와 해마체에서 다소 높았고 미크로좀분획의 활성은 뇌의 모든 부위에서 가장 낮은 분포를 보였으며 부위에 따라 뚜렷한 차이가 없었다. 뇌 ALDH는 isoelectric focusing에 의해 두 분획(E1, E2)으로 구성된 $low-K_m$ isozyme과 활성이 미약한 $high-K_m$ isozyme으로 분리되었으나 부위에 따른 분포양상의 차이는 관찰되지 않았다. This study was attempted to examine the ALDH activities using several aldehydes including synthetic biogenic aldehydes and the subcellular distribution in seven different areas of rat brain. The total ALDH activity with indole-3-acetaldehyde were higher than acetaldehyde and propionaldehyde in all areas. Taking $V_{max}/K_{m}$ ratio as the index of affinity of substrate to the enzyme, affinities of $low-K_m$, ALDH for indole-3-acetaldehyde and propionaldehyde were stronger than those of $high-K_m$, ALDH. Especially striatum showed the lowest $K_m$ value and the highest $V_{max}/K_{m}$ ratio for aromatic aldehyde and contained the highest activity with biogenic aldehyde such as DOPAL. From these results, it is suggested that brain low $K_m$ ALDH may play an important role in degradation of neurotransmitter-derived aromatic aldehydes, especially dopamine oxidation in the striatum. ALDH activity of mitochondrial fraction was slighltly high in the hypothalamus and hippocampus. Microsomal ALDH activity was lowest in all brain areas and a significant regional difference was not found. Although two $low-K_m$, isozymes, composed of cytosolic E1 and mitochondrial E2, and very weak high $K_m$ isozymes were identified by isoelectric focusing, their distribution pattern in various areas was not different.
서혜부 탈장 및 양성 항문 직장 질환 수술후 배뇨곤란에 영향을 주는 인자에 관한 분석
이영돈,이정남 대한소화기학회 1992 대한소화기학회지 Vol.24 No.5
Total 213 patients who had undergone surgery for inguinal hernia and benign anorectal disease were reviewed retrospectiveiy to determine the incidenc of postoperative urinary retention requring catheterization and to assess possible influences on that incidence. The age group and sex of patients and the amount of fluids administered perioperatively did not affect the rate of the retention. However, the incidence under the spinal anesthesia was significantly high (p<0.00001) compared to the incidence under the general anesthesia. In addition, there was a difference in the incidence of the retention related to the site of operation (p=0.0007).
Paenibacillus polymyxa and Burkholderia cepacia Antagonize Ginseng Root Rot Pathogens
이영돈,Khalid Abdullah Hussein,주진호 한국토양비료학회 2017 한국토양비료학회지 Vol.50 No.6
To isolate rhizobacteria exhibiting antifungal activities for for five pathogenic fungi (Sclerotinia sclerotiorum,Fusarium solani, Collectotricum gloeosporides, Fusarium oxysporum, and Botrytis cinerea) which causedamage to Ginseng root in Ginseng grown fields, four soils were collected from Cheorlwon gun, in Korea. From 4 soils, a total of 160 bacterial strains were isolated by dilution plate method. Among 160 strains, 32strains showed antifungal activities for one or more pathogens. From 32 strains, three strains exhibitedantifungal activities for all pathogens. These are two Burkholderia cepacia (ATCC 25416 and ET 13) and onePaenibacillus polymyxa (ATCC 842). These potent antifungal strains showed high identities (99% using16S-rRNA sequencing).
이영돈,유재홍,주진호 한국토양비료학회 2017 한국토양비료학회지 Vol.50 No.6
Treatment of food waste is becoming a big issue due to their significant quantities. Composting could be aneffective alternative for food waste management which could be used as soil conditioner or fertilizer with littleconcerns about heavy metals and pathogens. The purpose of this research was to evaluate the effect of foodwaste on Chinese cabbage growth and soil properties. 9 different treatments (two livestock manures, two foodwastes, two livestock manures + chemical fertilizer, two food wastes + chemical fertilizer, and control) wereapplied to Chinese cabbage. All treatments were carried out in 3 replicates. We measured leaf length, leafwidth, fresh weight, dry weight, and leaf greenness of Chinese cabbage. Treatment of one of food wastecomposts significantly increased leaf length and leaf width of Chinese cabbage by 28.6, 26.6, 67.7, and59.9%, respectively, in comparison to those of control, while no significant differences for leaf greennesswere shown. Application of food waste compost resulted in significant increase of EC, available P2O5, CEC,organic matter, and exchangeable cations. However, further researches are needed to reduce NaCl content offood waste.