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Park, Hyeong Cheol,Kim, Man Lyang,Kang, Yun Hwan,Jeong, Jae Cheol,Cheong, Mi Sun,Choi, Wonkyun,Lee, Sang Yeol,Cho, Moo Je,Kim, Min Chul,Chung, Woo Sik,Yun, Dae-Jin Springer-Verlag 2009 Molecules and cells Vol.27 No.4
<P>The transcription of soybean (Glycine max) calmodulin isoform-4 (GmCaM-4) is dramatically induced within 0.5 h of exposure to pathogen or NaCl. Core cis-acting elements that regulate the expression of the GmCaM-4 gene in response to pathogen and salt stress were previously identified, between -1,207 and -1,128 bp, and between -858 and -728 bp, in the GmCaM-4 promoter. Here, we characterized the properties of the DNA-binding complexes that form at the two core cis-acting elements of the GmCaM-4 promoter in pathogen-treated nuclear extracts. We generated GUS reporter constructs harboring various deletions of approximately 1.3-kb GmCaM-4 promoter, and analyzed GUS expression in tobacco plants transformed with these constructs. The GUS expression analysis suggested that the two previously identified core regions are involved in inducing GmCaM-4 expression in the heterologous system. Finally, a transient expression assay of Arabidopsis protoplasts showed that the GmCaM-4 promoter produced greater levels of GUS activity than did the CaMV35S promoter after pathogen or NaCl treatments, suggesting that the GmCaM-4 promoter may be useful in the production of conditional gene expression systems.</P>
Characterization of a stamen-specific cDNA encoding a novel plant defensin in Chinese cabbage
Park, Hyeong-Cheol,Kang, Yun-Hwan,Chun, Hyun-Jin,Koo, Ja-Choon,Cheong, Yong-Hwa,Kim, Cha-Young,Kim, Min-Chul,Chung, Woo-Sik,Kim, Jong-Cheol,Yoo, Jae-Hyuk,Koo, Yoon-Duck,Koo, Sung-Chul,Lim, Chae-Oh,Lee Plant molecular biology and biotechnology research 2002 Plant molecular biology and biotechnology research Vol.2002 No.-
We isolated a stamen-specific cDNA, BSD1(Brassica stamen specific plant defensin 1)that encodes a novel plant defensin peptide in Chinese Chinese cabbage (Brassica campestris L. ssp. pekinensis). Plant defensins are antimicrobial peptides containing eight highly conserved cysteine residues linked by disulfide bridges. In BSD1, the eight cysteine residues and a glutamate residue at position 29 are conserved whereas other amino acid residues of the plant defensins consensus sequence are substituted. BSD1 transcripts accumulate specifically in the stamen of developing flowers and its level drops as the flowers mature. The recombinant BSD1 produced in Escherichia coli showed antifungal activity against several phytopathogenic fungi. Furthermore, constitutive over-expression of the BSD1 gene under the control of the cauliflower mosaic virus (CaMV) 35S promoter conferred enhanced tolerance against the Phytophthora parasitica in the transgenic tobacco plants.
Park, Hyeong-Cheol,Kim, Man-Lyang,Kang, Yun-Hwan,Jeon, Joo-Mi,Yoo, Jae-Hyuk,Kim, Min-Chul,Park, Chan-Young,Jeong, Jae-Cheol,Moon, Byeong-Cheol,Lee, Ju-Huck,Yoon, Hae-Won,Lee, Sung-Ho,Chung, Woo-Sik,Li Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-
The Ca^(2+)-binding protein calmodulin mediates cellular Ca^(2+) signals in response to a wide array of stimuli in higher eukaryotes. Plants express numerous CaM isoforms. Transcription of one soybean (Glycine max) CaM isoform, SCaM-4, is dramatically induced within 30 min of pathogen or NaCl stresses. To characterize the cis-acting element(s) of this gene, we isolated an approximately 2-kb promoter sequence of the gene. Deletion analysis of the promoter revealed that a 130-bp region located between nucleotide positions -858 and -728 is required for the stressors to induce expression of SCaM-4. A hexameric DNA sequence within this region, GAAAAA (GT-1 cis-element), was identified as a core cis-acting element for the induction of the SCaM-4 gene. The GT-1 cis-element interacts with an Arabidopsis GT-1-like transcription factor, AtGT-3b, in vitro and in a yeast selection system. Transcription of AtGT-3b is also rapidly induced within 30 min after pathogen and NaCl treatment. These results suggest that an interaction between? GT-1 cis-element and a GT-1-like transcription factor plays a role in pathogen- and salt-induced SCaM-4 gene expression in both soybean and Arabidopsis.
내부마케팅과 고객지향성의 관계에 있어 조직동일시의 매개효과
강형철(Kang, Hyeong-cheol),곽대영(Kwak, Dae-Young) 대한관광경영학회 2013 觀光硏究 Vol.28 No.1
본 연구는 카지노 기업이 내부마케팅을 실행하였을 경우 조직구성원의 조직동일시와 고객지향성에 미치는 영향관계를 실증분석하고, 이러한 분석을 통해 효과적인 내부마케팅 전략을 위한 시사점을 제시하는데 목적이 있다. 본 연구의 실증분석 결과는 다음과 같다. 첫째, 카지노 직원을 내부고객으로 경영층지원, 권한위임, 교육훈련, 보상시스템, 내부의사 소통 등 내부마케팅 활동을 지속적, 효율적으로 실행하면 조직구성원들의 조직동일시는 높아지고 결과적으로 고객지향성이 높아져 외부 고객만족의 극대화를 실현시키는 동기부여의 효과가 있음을 알 수 있었다. 둘째, 카지노 기업의 내부 마케팅 활동이 카지노 직원들의 고객지향성에 미치는 직접적 영향보다 조직동일시를 매개하여 형성되는 고객지향성 효과가 더 강함을 알 수 있었다. 셋째, 경영층 지원, 권한위임, 교육훈련, 내부 의사소통 등은 조직동일시의 매개효과에 있어 부분매개를 하고 있는 것으로 나타났으며 보상시스템은 조직동일시를 통해서만 고객지향성에 영향을 미치는 완전 매개를 하는 것으로 나타나 내부마케팅과 고객지향성과의 관계에서 조직동일시의 매개역할을 확인할 수 있었다. The objectives of this study are to investigate and analyze mediating effects of organizational identification on the relationship between internal marketing and customer orientation. To achieve these objectives, as a conceptual framework of the study, the literature on internal marketing, organizational identification, customer orientation, and the relationships between them were reviewed. Moreover, and the empirical studies on employees’ perceptions in domestic Casino companies toward the above three factors were conducted. Based on the findings of this study, the following suggestions were presented to the domestic Casino companies. First, Casino companies need to perform differentiated human resources management by using the internal marketing factors that have positive effects on organizational identification and customer orientation. Second, based on the finding that organizational identification and customer orientation are seriously influenced by internal communication activities, Casisno companies are required to implement internal communication systems and manage them carefully. Third, in order to enhance customer service level, Casino companies need to keep providing employees with education and training opportunities. Fourth, according to the finding that promotion system has direct influences on employee’s organizational identification and customer orientation, Casino companies should operate them fairly and impartially.
Hyeong Dong Yuk,Minyong Kang,Jung Keun Lee,Sung Kyu Hong,Ja Hyeon Ku,Seok-Soo Byun,Cheol Kwak,Hyeon Hoe Kim,Sang Eun Lee,Chang Wook Jeong 대한비뇨기종양학회 2017 대한비뇨기종양학회지 Vol.15 No.3
Purpose: To evaluate the clinicopathologic and oncological outcomes of advanced metastatic testicular cancer in Korean men who underwent retroperitoneal lymph node dissection (RPLND) following chemotherapy. Materials and Methods: Data of 26 patients with testicular cancer who underwent RPLND after chemotherapy at 2 hospitals in Korea between September 2004 and June 2016 were retrospectively analyzed. Clinical and histopathological variables such as stage of the testicular cancer, age of the patients during surgery, size of the retroperitoneal lymph nodes (RPLNs), histopathological results, duration and complications related to the surgery, cancer recurrence, and mortality were analyzed. Results: During testicular surgery, the T stage was pT1, pT2, and pT3 in 50% (n=13), 26.9% (n=7), and 15.3% (n=4) of the patients, respectively. Mixed germ cell tumor was the most common finding, seen in 73.1% (n=19) of patients. The indications for RPLND were residual lymph nodes after chemotherapy, 84.6% (n=22); and disease progression and remission, 7.7% (n=2). Pathological analysis revealed viable tumors in 19.2% of patients (n=5), necrotic/fibrotic tissue in 42.3% (n=11), and teratoma in 34.6% (n=9). Intraoperative and postoperative complications occurred in 23.1% (n=6) and 19.2% of patients (n=5). The median duration of follow-up was 27.5 months (interquartile range, 1.3–108.2 months); 11.5% (n=3) patients had recurrence, and 3.8% (n=1) died of progressive metastatic testicular cancer. Conclusions: Viable germ cell tumors were present in 19.2% of patients with testicular cancer who underwent RPLND after chemotherapy. This is the first study of its kind in the Korean population.
Kang, Sung-Sik,Cho, Sang-Rae,Kim, Ui-Hyung,Park, Chang-Seok,Kim, Hyeong-Cheol,Chung, Ki-Yong,Lee, Seok-Dong,Jang, Sun-Sik,Jeon, Gi-jun,Kim, Sidong,Lee, Myeong-Suk,Yang, Byoung-Chul The Korean Society of Embryo Transfer 2016 한국동물생명공학회지 Vol.31 No.2
The recovery of epididymal sperm in animals is considered as one of the important tools to preserve high value or endangered species. However, there are no appropriate castrating indicators such as months of age in bull, sperm morphology, and motility, particularly in young Korean native bull (Hanwoo). Therefore, this study aimed to investigate sperm number, morphology, and motility of sperm in the epididymis tail of young Hanwoo bulls at 8 and 15 months of age. After castration, epididymal tails were collected and minced with blades to recover sperm. In experiments 1 and 2, sperm number, morphology, and motility were examined. Total number of sperm and percentage of normal sperm from bulls at 8 months of age was lower than that of bulls at 15 months of age after collection (P<0.05). Percentage of abnormal head, tail, proximal cytoplasmic droplet, dead and damaged acrosome of sperm from bulls at 8 months of age were higher than those of bulls at 15 months of age (P<0.05). In experiment 3, sperm motility from bulls at 8 and 15 months of age were examined before freezing and after thawing. Frozen-thawed sperm at 8 months of age showed low total motility and motile sperm with ${\geq}25{\mu}m/sec$ compared to those at 15 months of age and commercially-used sperm (P<0.05). In conclusion, sperm derived from the epididymal tail of bulls at 8 months of age showed high abnormal morphology and poor motility, which are not adequate for AI and IVF. On the other hand, sperm derived from the epididymal tail of bulls at 15 months of age showed high normal morphology and motility.
SAT-based unbounded symbolic model checking
Kang, Hyeong-Ju,Park, In-Cheol IEEE 2005 IEEE transactions on computer-aided design of inte Vol.24 No.2
This paper describes a Boolean satisfiability checking (SAT)-based unbounded symbolic model-checking algorithm. The conjunctive normal form is used to represent sets of states and transition relation. A logical operation on state sets is implemented as an operation on conjunctive normal form formulas. A satisfy-all procedure is proposed to compute the existential quantification required in obtaining the preimage and fix point. The proposed satisfy-all procedure is implemented by modifying a SAT procedure to generate all the satisfying assignments of the input formula, which is based on new efficient techniques such as line justification to make an assignment covering more search space, excluding clause management, and two-level logic minimization to compress the set of found assignments. In addition, a cache table is introduced into the satisfy-all procedure. It is a difficult problem for a satisfy-all procedure to detect the case that a previous result can be reused. This paper shows that the case can be detected by comparing sets of undetermined variables and clauses. Experimental results show that the proposed algorithm can check more circuits than binary decision diagram-based and previous SAT-based model-checking algorithms.
Approaches to avoid corruption of CAD models in configuration design method for solid rocket motors
Kang-Soo Lee,Won-Hoon Kim,Bang-Eop Lee,Do Hyeong Lee,Tae-Kyung Hwang,Yeon-Cheol Kim,Seong-Gyo Jang,Byeong-Wook Choi,Jun-Hui Shin,Hyuk-Sun Kwon,Dong-Pil Shin,Sung Hwan Kim 한국생산제조학회 2012 한국생산제조시스템학회 학술발표대회 논문집 Vol.2012 No.10