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백혜자,이다솜 한국발생생물학회 2019 발생과 생식 Vol.23 No.2
This study focused on the association of prostaglandins and a progestin, 17α, 20β-dihydroxy-4-pregnen-3-one (17α20βP) during the ovulation process in longchin goby, Chasmichthys dolichognathus. We performed several in vitro experiments using 850–920 μm diameter oocytes which were at the migratory nucleus stage. With the 890–920 μm diameter oocytes, no significant difference in ovulation was observed in any of the prostaglandins (PGE1, PGE2, and PGF2α) treated groups although PGE2 and PGF2α at concentrations of 50 ng/mL increased ovulation slightly compared with controls; however, 17α20βP production was stimulated with PGE1 alone at low concentrations (5 ng/mL). In 850 μm diameter oocytes, PGF2α at concentrations of 50 and 500 ng/mL resulted in a significant increase in ovulation. 17α20βP (50 ng/mL) alone had no observable effect on ovulation, but in the combined of PGF2α 50 or 500 ng/mL it caused the greatest effect on ovulation. The sensitivity of oocytes to the induction of ovulation varies between 850 and 890–920 μm, it appeared to vary depending on the migration status of nucleus. These results suggest that PGF2α (or combined of 17α20βP) was more potent in inducing ovulation of the longchin goby.
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백혜자,황인준,박명희,김형배 한국수산과학회 2009 Fisheries and Aquatic Sciences Vol.12 No.4
Several studies have reported that nonylphenol (NP) and 2,2’,4,6,6’-pentachlorobiphenyl (PCB104) exhibit estrogenic activity. To investigate the estrogenic potency of NP and PCB104 during oocyte maturation, fully vitellogenic oocytes (0.76-mm diameter in average) of yellowfin goby, Acanthogobius flavimanus, were exposed in vitro to these chemicals at different concentrations (0.1, 1, 10, 100, and 1,000-ng/mL) with the exogenous precursor 17α-hydroxyprogesterone (17αOHP) 50 ng/mL in the presence or absence of human chorionic gonadotropin (HCG). The production of testosterone (T), estradiol-17β (E2), and 17α,20β-dihydroxy-4-pregnen-3-one (17α20βOHP) in response to NP or PCB104 were measured by radioimmunoassay. Steroid levels were also expressed as E2/T and E2/17α20βOHP ratios. In the absence of HCG, no significant differences in either NP or PCB104 treatment groups were observed. In the presence of HCG, NP treatment did not show significant differences in the production of T, E2, and 17α20βOHP at any concentrations tested, but E2/T ratios were decreased at concentrations of 0.1, 1, 10, and 1,000 ng/mL compared with the control group. PCB104 decreased E2 production at concentrations of 0.1, 10, and 1000 ng/mL, but did not show significant differences in the production of T and 17α20βOHP at any concentration tested. While E2/T ratios were decreased at PCB104 concentrations of 0.1, 1, 10, and 1,000 ng/mL, E2/17α20βOHP ratios were also decreased at 0.1, 10, and 1,000 ng/mL compared with the control. Results indicate that both NP and PCB104 appeared to have antiestrogenic effects during this phase.
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백혜자 中央醫學社 1990 中央醫學 Vol.55 No.1
The study is aimed at exploring recognized nursing concept through nursing business in the spot of nursing care. The data were collected from 118 nurses conveniently selected from the population of nurses working in Kang Won proving, from July 20 to August 20,1989. for one month. The main result of this study can be summarized as follows; 1. In general characteristic the age distribution of examined respondents is the largest in number from 20 year old to 34 year old (79.7 %). The ratio of educational level was 91.5 % in nursing college graduation. The job sites were 32.2 % of respondents in hospital, 43.2 % of them in public health center and medical office, nurse teacher (18.6 %), industrial sites (5.9 %) respectively. Nurses with over five-year career were 53 %. Religion showed 37.3 % of Protestant, 14.4 of Catholic and 13.6 % of Buddhism. Marital status showed 48.3 % in marriage and 50.9 in single. 2. Nurse teachers (59.1 %) and nurses working in public health center and medical office (45.1 %) relatively highly recognized nursing service as technical profession with originality. Nurses working in hospital (39.5 %) and industrial sites (14.2 %) recognized at a low degree. In general most respondents (44.1 %) regarded nursing job as automomous technical profession. A difference of view in each nursing field didn't pay attention (p < 0.05). 3. Nursing laid an emphasis on the sot of nursing care was carried out at hospital (100 %), public health center and medical office (90.2 %), nurse teacher (86.47o), and all mentioned were executed in physical side. In every domain nursing execution on social (48.3 %) and spiritual side (33.9 %) was neglected. 4. According to responded survey deficient quality as nurse was technical knowledge -77-in hospital (57.9 %), industrial sites (57.1 %), and was sincerity and responsibility.(35.2 %) in public health center and medical office, and was satisfaction (27.2%) and technical knowledge (27.2 %) concerning nurse teacher. Besides, response about deficient quality were different in each field (p < 0.05). 5. On clients of nursing care, respondents answered that in hospital nursing care was carried out at the lowest degree for family (39.5,7o), group (23.7 %). Nursing care to nurses themselves (54.9 %), group (75.6 %) was executed at a relatively low degree in public health center and medical office. In school nursing care was performed at a lower degree for guardian (40.9 %) group or nurses themselves (14.2 %), too. 6. As regards nursing activity with important aim in the spot of nursing care, the high degree of nursing activities consisted of cooperation of curing disease (89 %), health education (83.0 %) and decreasing of pain of clients. On the other hand low degree of nursing activities were disease prevention (69.5%), maintaining health (63.617o) and health promotion (62.7 %) 7. Among Maslow's human needs, the order showing the lowest interests during the nursing care can be summarized as follows; self-realization (72.0 %), esteem need (44.9 %), need for love (42.7 %); that is to say, nursing care for satisfying the high rank need has the lowest interest. 8. In the spot of nursing care, in order to satisfy nursing care needs of Virginia Henderson's basic needs this study was done to investigate the degree of nursing care execution. In every items the ratio of nursing care execution was low; in particular in execution of nursing care for satisfying the high rank needs such as Preparation for recreation time (2.29 ± 1.065), Through work that is suitable for health, help was given with a view to feeling achievement (2.364 ± 1.091) Help is given to clients for recreational activities (2.525 ± 1.167), Psychological knowledge for clothing is put into use in nursing care (2.661 ± 1.094). Besides, Nursing care for normal excretion (2.574 ± 1.408), and Airpollution Prevention(2.873 ± 2.561) were low, too.
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백혜자,김형배,노섬,이영돈,박무억 한국수산과학회 2004 Fisheries and Aquatic Sciences Vol.7 No.1
Plasma steroid hormone levels in the viviparous rockfish (Sebastes schlegeli) were examined in relation to gonadal histology under controlled photoperiods and water temperatures. To investigate those effects in S. schlegeli the photoperiod was maintained at 15L:9D in June and then it was gradually decreased to 9L:15D in October. It was then gradually increased to 12L:12D in January, followed by 14L:10D in February. The water temperature was 19-20℃ in July. From August to October, it was from 18℃ to 12℃. Then, it was dropped to a low of 9-11℃ in November to December and then gradually increased to 14-15℃ in February. In females, both plasma estradiol-17β (E2) and testosterone (T) levels from August to February showed a similar pattern in both the treatment and the control groups. In the treatment group, the peaks of plasma E2 and T were observed in November, and the peaks were closely correlated to histological observations. Oocytes contained many yolk globules (final vitellogenic oocytes), and oocytes at the migratory nucleus stage increased in size. Plasma levels of progesterone did not change much throughout the experimental period. However, in the control group, the peaks of E2, T, and progesterone were observed in February. These results indicate that the controlled photoperiod and water temperature accelerated sexual maturity, corresponding to the advancement of plasma E2 and T peaks by approximately 3 months. In males, plasma T levels showed a similar pattern from August to October in the treatment and control groups, though levels in the treatment group were higher than those in the control group. From histological observations, the treatment group copulated one month earlier.
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동자개(Pseudobagrus fulvidraco)의 난모세포 성숙과 배란에 대한 스테로이드와 HCG의 in vitro효과
백혜자,한창희,임창구 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1
The aim of this study was to determine the effect of steroids and human chorionic gonadotropin(HCG) on in vitro maturation and ovulation of oocyte in Pseudobagrus fulvidraco. Oocytes were incubated in the media Leibovitz L15 supplemented with the various concentration of 17α, 20β-dihydroxy-4-pregnen-3-one (17α20βOHP), 17α-hydroxyprogesterone (17αOHP), progesterone(P₄), estradiol-17β(E₂)and HCG. After 60 hours incubation, the maturation ability of oocyte was assessed by the appearance of germinal vesicle breakdown (GVBD). GVBD was significantly enhanced by the addition of 17α20βOHP, 17αOHP, P₄and HCG(P$lt;0.05). The highest GVBD was observed when 17α20βOHP and HCG were supplemented to media. When oocytes were cultured for 16hours in media containing 10∼1,000 ng/ml 17α20βOHP, 17αOHP and P₄, the rate of GVBD in oocytes cultured in the medium supplemented with 100ng/ml 17α20βOHP (65%) was significantly higher than that with 17αOHP(40%) and P₄(35%). The effects of 17α20βOHP and HCG on GVBD were assessed by various concentration of these hormones. When oocytes were cultured for 60 hours in various media containing 1∼1,000 ng/ml 17α20βOHP or 5∼1,000 IU/ml HCG, the GVBD of oocytes was significantly increased in the medium with 10∼100 ng/ml 17α20βOHP and 500 IU/ml HCG. When oocytes were cultured in the various media supplemented 1∼1,000 ng/ml 17α20βOHP or 5∼1,000 IU/ml HCG for 60 hours, the media with 1∼100 ng/ml 17α20βOHP or 50∼1,000 IU/ml HCG significantly increased in the rate of ovulation. However supplementation with 1,000 ng/ml 17α20βOHP or 5 IU/ml HCG did not improve the rate of ovulation compared to controls. This results indicate that supplementation of steroid and HCG except E₂can improve the in vitro maturation and ovulation of oocyte in P. fulvidraco; HCG and 17α20βOHP may be more effective than other steroids on oocyte maturation and ovulation in P. fulvidraco.
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범가자미, Verasper variegatus의 난황단백질 분리 및 면역학적 특성
백혜자,한창희,김우진,박중연,김진한,김윤 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1
The yolk protein of spotted flounder, Verasper variegatus was purified by precipitation with cold distilled water, followed by Sepharose CL-6B column chromatography. The purified protein was identified as vitellin by Ouchterlony's immunodiffusion test and immunoelectrophoresis. The purified vitellin from ovarian crude extracts has same antigenic determinants with the female specific serum protein, vitellogenin. The molecular weight of purified vitellin was estimated about 550kD by gel filtration. The vitellin was composed of three major subunits with molecular weight of about 108, 85 and 31 kD, and two minor subunits. The vitellin was identified by western blot analysis using anti-vitellin antibody.
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