돼지 생식기호흡기증후군 바이러스의 Nucleocapsid 단백질 발현 및 진단적 응용

박효선,한태욱,김현수,최강석,이은정,강신영,Park, Hyo-Sun,Hahn, Tae-Uook,Kim, Hyun-Soo,Choi, Kang-Seuk,Lee, Eun-Jeong,Kang, Shien-Young 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.1

Porcine reproductive and respiratory syndrome (PRRS) is characterized by reproductive failures in sows and respiratory problems in piglets. The nucleocapsid(N) protein, encoded by the open reading frame 7 (ORF7) gene, is known to be the most abundant and antigenic protein in PRRS virus. Therefore, it was suggested that the N protein could be a suitable candidate for the detection of PRRS virus-specific antibodies and diagnosis of PRRS. In the present study, the ORF7 gene encoding the N protein was cloned and expressed as a fusion protein with the glutathione S-transferase (GST) in Escherichia coli. The resulting GST-N recombinant protein was used as an antigen for an indirect sandwich enzyme-linked immunosorbent assay (i-ELISA). Expressed GST-N recombinant protein was migrated at 41 kDa and reacted with ORF7-specific monoclonal antibody by Western blotting. In order to increase the specificity of the ELISA for the detection of PRRS virus-specific antibodes, an i-ELISA was developed using an anti-GST antibody as a capture antibody. The sensitivity and specificity of developed i-ELISA were 92% and 96%, respectively. Based on these results, it was suggested that the i-ELISA is a simple and rapid test for screening a large number of swine sera for the anti-PRRS virus antibodies.

평면 비정형 구조물에 적용된 중간층 면진 시스템의 지진 응답 제어 성능 분석

박효선,김현수,강주원,Park, Hyo-Sun,Kim, Hyun-Su,Kang, Joo-Won 한국공간구조학회 2019 한국공간구조학회지 Vol.19 No.2

In this study, the seismic response is investigated by using a relatively low-rise building under torsion-prone conditions and three seismic loads with change of the location of the seismic isolation system. LRB (Lead Rubber Bearing) was used for the seismic isolator applied to the analytical model. Fixed model without seismic isolation system was set as a basic model and LB models using seismic isolation system were compared. The maximum story drift ratio and the maximum torsional angle were evaluated by using the position of the seismic layer as a variable. It was confirmed that the isolation device is effective for torsional control of planar irregular structures. Also, it was shown that the applicability of the mid-story seismic isolation system. Numerical analyses results presented that an isolator installed in the lower layer provided good control performance for the maximum story drift ratio and the maximum torsional angle simultaneously.

돼지 써코바이러스 2형의 진단을 위한 PCR법 적용

박효선 ( Hyo Sun Park ),이효상 ( Hyo Sang Lee ),나기복 ( Ki Bok Na ),이관복 ( Kwan Bok Lee ),강수정 ( Su Jung Kang ),문순화 ( Sun Hwa Moon ) 한국가축위생학회 2006 韓國家畜衛生學會誌 Vol.29 No.1

Porcine circovirus (PCV) is a small, nonenveloped virus that contains a single-stranded circular DNA genome of about 1.76kb and belongs to the family circoviridae. The PCV-2 has been incriminated as the cause of post-weaning multisystemic wasting syndrome(PMWS), an emerging disease in pigs. In the present study, a PCR assay was applied to detect PCV-2 in tissue samples. The presence of PCV-2 antigen in the porcine tissues was confirmed by indirect immunofluorescence(IIF) with PCV-2 specific monoclonal antibodies. And then DNA extracted from PCV-2 positive tissues was used as a template. One oligonucleotide primer suitable for PCR was selected from a published PCV-2 sequence(Genbank). Amplified PCR product was detected the same fragment lengths of 416bp as a control. Based on these results, it was suggested that the PCR is a simple and sensitive method for support diagnostic purposes.

군 인적자원개발정책 결정과정에 관한 연구

박효선 ( Hyo Sun Park ) 한국인력개발학회 2010 HRD연구 Vol.12 No.4

본 연구의 목적은 군 인적자원개발정책이 국가적인 정책으로 발전하는 과정에서 관련 정부부처 및 국회, 연구기관, 각종 위원회 등의 역할과 갈등해결, 정책결정요인 등을 분석하여 향후 정책발전에 필요한 자료를 제공하는데 있다. 이 연구는 Campbell의 모형을 분석틀로 하여 군 인적자원개발 결정과정을 제1단계: 정책의 시동기(환경), 제2단계: 정책의 협력기(운동), 제3단계: 정책의 갈등기(전환), 제4단계: 정책의 결정기(산출)로 구분하여 분석하였다. 연구결과 병영문화개선과 군내 평생학습시대의 도래와 함께 군 조직의 학습체제 구축을 갈망하고 있는 군과 국민적인 요구를 정치적, 사회적으로 수용하여 국가정책으로 결정된 것으로 나타났다. 또한 다수의 정책행위자들이 군 장병의 자기개발을 통한 국가 인적자원개발의 기여라는 대의명분과 군 장병의 욕구 및 국민적 기대에 부응하여 정책이 결정되었다. 향후 민·관·학·연과 연계한 단체의 역할이 갈수록 커지는 가운데 군 전투력 증대와 국가발전이라는 공동의 목표가 실현되도록 이들 단체들과의 지속적인 유대관계는 물론 지속적인 연구와 평가가 이루어져야 한다. This research`s purpose is to provide data for the policy development hereafter through analyzing the roles of government authorities, congress, research institute and diverse com mittee, the conflict settlement, the factor of policy decision in the process of developing nat ional policy. The military human development policy is promoting combat strength through making i ncessant study conditions and is expediting nationally in order to contribute to national hu man resource development. This policy began to improve soldier`s personal development at the situation of thinking negatively about military tenure. It is decided on a national cours e of action because both military and nations want to establish study system for military culture development and coming of permanent study era. Besides government authorities, congress, research institute, diverse committee which parti cipate in many policy votes decided this policy because it is satisfied by soldier`s need and nation`s expection. Hereafter the bigger roles of corporation nation-government-education-rel ation corporations get, the more it doesn`t need continuous relationship but also substantial promoting of military human resource development so as to increase combat strength and national force.

해방 후 창군기 한국군의 평생교육 경향분석

박효선(Park Hyo-Sun) 한국평생교육학회 2007 평생교육학연구 Vol.13 No.4

  이 연구는 창군기인 해방 이후부터 1960년까지 군에서 추진되어 온 군 평생교육 경향을 고찰해 봄으로써 그 역할과 의의를 새롭게 조명해 보고자 하였다. 그동안 군의 평생교육에 관한 역사적인 가치를 발견하고자 하는 연구는 군의 폐쇄적 특성과 자료접근의 한계성 등으로 전무하였다. 이러한 점에서 징병제를 채택하고 있는 국가로서 군 평생교육은 성인교육의 차원을 떠나 개인은 물론 국가발전과 경제·정치·문화 등에 미치는 영향평가와 시대적 경향분석은 매우 의미가 있다고 하겠다. 해방 후 미군정기를 거쳐 한국전쟁과 전후복구 등 격동기에 국방체계를 정립하는데 중요한 정책적 수단과 동력이 된 평생교육의 주요경향은 다음과 같이 요약된다. 첫째, 이념적 측면에서 국가적 기본이념인 자유민주주의 체제의 수호와 국방정책의 목표인 의존적 자주국방을 구현하기 위한 반공 민주군대로서 정신무장의 수단으로 활용하였다. 둘째, 성인 기초교육과 학교보완형 사회교육과 더불어 미국식 민주주의를 뿌리내리기 위한 시민교육의 특징이 있다. 셋째, 평생교육 체제 측면에서 외세의 힘과 관이 주도하는 형태로서의 교육적 특성을 보인다. 결론적으로 창군기의 군 평생교육은 국민으로서 필요한 기본적 자질을 부여해 주는 사회화 또는 국민교육도장으로서도 역할과 초창기 정신교육 및 군심결집에 핵심적 역할을 수행하였음을 시사한다.   The purpose of Military training exercise is to effectively carry out the missions of the military; however, as the enlisted members become discharged and return to society, the skills obtained through training exercise play a vital role on personal as well as social level. As a nation that is practicing a conscription system, the impact of the military"s life-long education system on our economy, politics and culture, let alone on individual growth, cannot be underestimated. The role of the military is bound to change depending on the phases of the times, as it is closely related with the degree of a nation"s development and the systematization of its military. In this paper, the role and the purpose of our military life-long education, from its inception till 1960, will be discussed by carefully examining the trends of the time. The Army"s role as an educational institution included anticommunist education, the eradication of illiteracy and the development of patriotic perspective, all of which had greatly contributed to the ideological cohesiveness and the combat strength of the military. The Military education had also helped improve the overall level of socialization and citizenship, two qualities intrinsic to the members of a developed society.

학술논문 5 : 한국군의 평생교육 변천과정 평가분석 연구

박효선 ( Hyo Sun Park ) 한국군사학회 2014 군사논단 Vol.77 No.-

항암화학요법을 받고 있는 유방암 환자에서 내관지압 방법에 따른 오심, 구토, 식욕부진의 효과 비교

박효선(Park, Hyo-Sun),신나연(Shin, Na-Yeon) 한국기초간호학회 2020 Journal of korean biological nursing science Vol.22 No.2

Purpose: The purpose of the study was to identify the comparison with the different methods of acupressure treatment in breast cancer patients undergoing chemotherapy. Methods: This study was a single group pretest-posttest design. Thirty patients age 30- 65 scheduled for chemotherapy were included. The data were collected through self- reported questionnaires on nausea, vomiting, and anorexia and analyzed using descriptive statistics and the Wilcoxon signed ranked test. Results: The relieving effect of nausea and vomiting (Z=-2.54, p=.011) was significant in P6 acupressure by wrist bands. Conclusion: Patients undergoing chemotherapy have relieving effects on nausea and vomiting after P6 acupressure by wrist band. This study demonstrates the stimulation of the P6 acupressure by wrist band for reducing nausea and vomiting for breast cancer patients undergoing chemotherapy.

중간층 면진시스템이 적용된 평면 비정형 구조물의 지진 응답 제어성능 분석

박효선(Park, Hyo-Sun),김유성(Kim, Yu-Seong),강주원(Kang, Joo-Won) 대한건축학회 2019 대한건축학회 학술발표대회 논문집 Vol.39 No.2

초등학교 방과후 영어 수업에 나타난 학생-교사 상호작용 연구 Vol.20 No4

박효선(Park Hyo sun),박선호(Park Seon Ho) 한국초등영어교육학회 2014 초등영어교육 Vol.20 No.4

This study aims to analyze interactions between teachers and students in the first and second graders' after-school classes. The data was collected through classroom observations and audiotapes. The researcher's field notes and interviews with five teachers(two English native teachers, two Korean English native teachers, and a Korean English teacher) were also included. The results are as follows. First, most utterances observed were initiated by the teachers mainly through giving information, asking display questions, and giving directions to students in after-school English classes. Second, the most frequent type of students' utterances was dependent on the talks led by the teachers. Students' utterances also tended to be more active especially when they express their opinions to the teachers and fellow students. Third, activities from supplementary materials, rather than those from the textbook only, influenced more positively on students' active talks and stimulated interactions between teachers and students. Finally, the native language of teachers influenced on their classroom English use despite the fact that all teachers had excellent English competence. Based on these findings some suggestions are made for the after-school English programs for lower grade students in the future.

대장암 노인 환자의 복강경 수술시 마취가스 가온가습요법의 효과

박효선(Park, Hyo-Sun),강윤희(Kang, Younhee) 한국성인간호학회 2018 성인간호학회지 Vol.30 No.2