競走馬에서의 藥物檢査

金尙塡 대한수의학회 1990 대한수의학회 학술대회발표집 Vol.1990 No.-

한우의 무릎관절 발생에 관한 조직학적 연구

백영기,이한경,양홍현,김인식,Paik, Young-ki,Lee, Han-kyoung,Yang, Hong-hyun,Kim, In-shik 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1

The morphogenesis of the stifle joint in Korean cattle embryos and fetuses was observed by radiography, alizarin red S stain and light microscopy. Fourty-eight(48) embryos and fetuses, ranging from 11mm to 160mm in crown-rump length (C-R L), were used for this study. The experimental samples were divided into twelve separate groups according to their C-R L. The first to the nineth group ranged from 11-100mm in C-R L, spaced at 10mm intervals. The tenth to the twelfth group ranged in C-R L from 101mm to 160mm, and were spaced at 20mm intervals. The results were as follows; 1. The first appearance of the interzone between the femur and tibia was formed in the second experimental group. The patellar ligament and cruciate ligament appeared in the third group and the patella, menisci and synovial cavity appeared in the fourth group. 2. The lateral and medial menisci were first obseved in the fourth group, and these structures showed crescent shape in the fifth group of the fetuses. 3. The first appearance of the femoro-tibial joint cavity was in the third group. The fifth group contained the first appearance of the patello-femoral cavity. Also, the femoro-tibial cavity was divided into two cavities by the anterior and posterior cruciate ligament.

진도견(珍島犬) 정세관상피(精細管上皮)의 정자발생(精子發生)과 미세구조(微細構造)

김용환,박영석,Kim, Yong-hwan,Park, Young-seok 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1

To investigate the cycle and relative frequences and the fine structure of seminiferous epithelia in mature Jindo dogs, histologic study was performed. The results obtained were summarized as follows; 1. Type A spermatogonia appeared approximately 1.6 times as many at stage II as compared to stage I while type In spermatogonia appeared small amount in stage III, IV and V. type B spermatogonia were found during the stage VI to VIII, though not detectable during stage I to V. The type B spermatogonia divided at stage VII to produce the preleptotene primary spermatocytes at stage VIII. The number of primary spermatocytes of the leptotene phase markedly increased during stage I to II, and the primary spermatocytes of the pachytene phase were shown the least in number at stage IV. The secondary spermatocytes could be seen only at stage IV. 2. The relative frequencies of each stage from stages I to VIII of the cycle of seminiferous epithelia were 31.6, 11.9, 10.0, 3.2, 8.2, 10.1, 11.7 and 13.2% respectively. 3. On electron microscopic observations, acrosomal vesicle of spermatids appeared larger though the bulk of germ cells were the morphologically same as those of the other animal species. Thread line structures light microscopically observed in the cytoplasm of Sertoli cell were the longitudinal orientation of mitochondria.

성장(成長) hormone이 랫드 소장상피세포(小腸上皮細胞) 재생주기(再生週期)에 미치는 영향에 대하여

곽수동,김종섭,안동원,Kwak, Soo-dong,Kim, Chong-sup,Ahn, Dong-weon 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1

The purpose of this study was to examine the effect of growth hormone(GH) on the division and migration of the small intestinal epithelium in mice. Twenty mice(ICR), weighing initially about 10 g, aged 18 days were randomly subdivided in two groups of control and GH-treated group. Control group was sacrificied at 2 hr, 2, 3, 4 and 5 day after intraperitoneal injection of $^3H$-thymidine($^3H$-TdR, $4{\mu}$ Ci/gm of BW/day for 2 days). GH-treated group was injected subcutaneously with somatotropin(10 IU/mouse/day for 4 days) from 2 day ago of first $^3H$-TdR injection and then was sacrificied at 2 hr, 2, 3, 4 and 5 day after intraperitoneal injection of $^3H$-TdR($4{\mu}$ Ci/g of BW/day for 2 days). The small intestines were collected for autoradiogrophy and Hematoxylin counterstain. The location of the labeled epithelial cells(LEC) in villi of the small intestine was invested with light microscope. 1. In the control group, the LEC regions in the small intestine were located at crypts on 1 dsy, at $0%{\sim}15.9{\pm}3.6%$ of villus high value(VHV) on 2 day, at $0%{\sim}49.8{\pm}16.5%$ of VHV on 3 day, at $0%{\sim}95.3{\pm}6.9%$ of VHV on 4 day and $62.9{\pm}16.7{\sim}100%$ of VHV on 5 day, respectively. 2. In the GH-treated group, the LEC regions in the small intestine were located at crypts on 1 day, at $0%{\sim}39.2{\pm}9.5%$ of VHV on 2 day, at $0%{\sim}81.5{\pm}18.2%$ of VHV on 3 day, at $45.2{\pm}11.5%{\sim}100%$ of VHV on 4 day, at 85%~100% of VHV on 5 day, respectively. 3. VHV of tops in the LEC regions appeared to be 23.3% and 31.7% higher on 2 and 3 day, and VHV of the LEC region bases appeared to be 45.2%, 22.1% higher on 4 and 5 day, respectively in the GH-treated group than those observed in the control group. These difference was very highly significant(p<0.01).

DNA 다형(多型)에 있어서 진도견(珍島犬)과 잡종견(雜種犬)과의 비교(比較)

한방근,김주헌,강주원,이케모토 시게노리,Han, Bang-keun,Kim, Joo-heon,Kang, Ju-won,Ikemoto, Shigenori 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1

Mitochondrial DNA(mt DNA) of Mammalian is the circular one which the 16.5K base pairs and show the maternal inheritance. Evolutional speed of nucleotide sequence is very fast. So that polymorphic analysis of mt DNA provide the useful informations to investigate the genetic relations of interspecies. Authors trials were focussed to compare with the polymorphic differences of mitochondrial DNA between Jindo and Japanese mongrel dogs. DNA was extracted from bloods of 21 head of Jindo dogs and 20 head of Japanese dogs and isolated using 10 kinds of restriction endonucleases(Apa I, BamH I, Bgl II, EcoR I, EcoR V, Hinc II. Hind III, Pst I, Sty I, Xba I) and then separated by the agarose gel electrophoresis. After sourthern blotting hybridization was completed using the mtDNA of Japanese mongrel dogs as a probe. Autoradiography was used to compare the polymorphism of mtDNA both dogs. The results obtained were as follows; 1. mt DNA of Jindo dog showed polymorphism resulting cleavage with four kinds of restriction endonuclease, Apa I, EcoR V, Hinc II, Sty I. While in the Japanese mongrel dogs observed the polymorphism in the five kinds of restriction endonuclease supplemented with EcoR I. 2. Compared with both dogs the frequency differences of DNA polymorphism were recognized in the specific restriction endonuclease Apa I. Consequently in the restriction endonuclease Apa I both dogs classified with three types as A, B, C however in the Jindo dogs frequency of C type was 71.5 percent but in Japanese mongrel dogs observed 45 percent in the A type. 3. DNA polymorphism obtained from the use of five kinds of restriction endonuclease were classified with seven types. In Jindo dogs frequency was highest in the type 6 as 71.4 percent but in the Japanese mongrel dogs showed 35 percent in the type 5. 4. Genetic distances calculated by NEI method showed 0.0089 in Jindo dogs and was 0.0094 in the Japanese mongrel dogs.

국내토양에서 분리한 혐기성 세균 Streptococcus sp. An-21-1 이 생성하는 항생물질 I. 혐기성 세균의 선별과 동정

박승춘,윤효인,오태광,Park, Seung-chun,Yun, Hyo-in,Oh, Tae-kwang 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1

Anaerobic bacteria are suggested to be potential source for new antibiotics. In order to search for antibiotics from domestic origin, we collected 800 soil samples across Korean locations and could isolate as many as 989 anaerobic strains. Among them 10, strains were found to have good producing capacity of antibiotics. An anaerobe was finally selected due to secreting antibiotics having high antimicrobial activity towards multiple resistant microorganism(E coli JM 83) transformed by genetic engineering technique. Its morphological, physiological and biochemical charateristics were investigated, together with antimicrobial spectrum therefrom. On antimicrobial spectrum study, substance secreted from this strain, had no activities to fungus and yeast. The selected strain showed G(+) and coccal shape, on Gram, staining and electron scanning microscopy, respectively. Biochemically this strain utilized glucose, fructose lactose, sucrose, but did not arabinose, cellulose, rhamnose, sorbitol, trehalose, mannitol. Catalase test showed negative property. Optimal growth temperature was $37^{\circ}C$. The results obtained above suggest this strain Streptococcus faecium subspp. and we named it Streptococcus sp. An-21-1.

국내토양에서 분리한 혐기성 세균 Streptococcus sp. An-21-1 이 생성하는 항생물질 II. 항생물질을 생성하는 혐기성 세균의 발효 및 항생물질의 분리 정제

박승춘,윤효인,오태광,Park, Seung-chun,Yun, Hyo-in,Oh, Tae-kwang 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1

In order to search for new antibiotics from anaerobic bacteria, a large number of samples from domestic soil were collected and processed by apropriate methods. A potential strain, Streptococcus sp. An-21-1, was found to produce antimicrobial compounds. The Results were as follows; 1. During fermentation, the bacteria grew rapidly up to 20hr, thereafter entered the death phase. The optimal temperature and pH for the bacterial growth were $37^{\circ}C$ and pH 7.0, respectively. 2. Antibiotics were purified from culture broth by solvent extraction, silica gel column chromatography and Sepadex L.H 20 column. 3. Physicochemical properties of Ap-1 and Ap-2 were similar ; Their melting points were between $234-237^{\circ}C$. Color reactions of ninhydrin, 2,7-dichlorofluorescein, 4-dimethylaminobenzaldehyde, Dragendroffs reagent and 20% $H_2SO_4$, were positive. Therefore, we assumed that these antibiotics have amine group, immine group, alkaloid, and lipid components. These were stable to heat. UV spectrophotometry showed two peaks at 210 nm and 260 nm. From above results, we assumed these antibiotics are belong to the peptide antibiotic family.

Xylazine의 진정효과와 α-adrenergic 수용체 봉쇄약물의 길항효과

김충희,하대식,김양미,김종수,Kim, Chung-hui,Hah, Dae-sik,Kim, Yang-mi,Kim, Jong-shu 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1

The central nervous system depressant effect of xylazine and xylazine-ketamine was studied in chicken and mice. Intraperitoneal injection of xylazine(1~30 mg/kg) and xylazine(1~30 mg/kg)-ketamine(100 mg/kg) induced a loss of the righting reflex in chicken and mice, respectively. These effects of xylazine were dose-dependent. The results obtained were as follows; 1. The effect of xylazine-induced depression was antagonized by adrenergic antagonists having ${\alpha}_2$-blocking activity(yohimbine, tolazoline, piperoxan and phentolamine). 2. Yohimbine was most effective in the reduction of the CNS depression by xylazine. 3. Phenoxybenzamine and prazosin did not reduced CNS depression by xylazine in both species. 4. Labetalol (${\alpha}_1$, ${\beta}_1$-adrenergic antagonist) and propranolol(${\beta}$-adrenergic blocking agent) were not effective in reducing xylazine induced depression. 5. Cholinergic blocking agents (atropine and mecamylamine), a dopaminergic antagonist (Haloperidol), a histamine $H_1$-antagonist(chlorpheniramine), a histamine $H_2$-antagonist(cimetidine), a serotonergic-histamine $H_1$ antagonist(cyproheptadine) were not effective in reducing xylazine-induced depression. 6. Xylazine-induced depression is mediated by ${\alpha}_2$-adrenergic receptors and appears not to be involved in cholinergic, dopaminergic, serotonergic or histaminergic pathways.

역전장 전기영동장치를 이용한 대형 DNA 분리에 관한 연구

주이석,윤용덕,Joo, Yi-seok,Casey, Thomas A,Yoon, Yong-dhuk 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1

Gel electrophoresis has proven to be one of the most useful of DNA separation and purification. The new technique of pulsed field gel electrophoresis (PFGE) is high resolution separation of large size DNA moleculs. Conventional continuous gel electrophresis can not be separation of large DNA fragments(20~50 k base). Field inversion gel electrophoresis(FIGE) is very useful for large DNA molecules. We have found that a pulse ratio ; 2 : 1, time ; 24hrs., volts ; $10^{volts}/_{cm}$, start ; 0.45sec, end ; 1sec, is most effectively resolves DNA fragment in the 6~50k base.

소 결핵균의 면역세포화학적 동정

김순복,서정향,문운경,Kim, Soon-bok,Sur, Jung-hyang,Moon, Oun-gyeong 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1

The present study was intended to use the avidin-biotin-peroxidase-antiperoxidase complex (ABPAP) method for the identification of Mycobacterium bovis in the tissue sections of infected cattle. Antibodies and linksera for ABPAP procedure used in incubated order were rabbit anti-Mycobacterium polyvalent antibodies, goat anti-rabbit IgG, rabbit peroxidase-antiperoxidase complex, biotinyl-horse anti-rabbit IgG, and avidin-biotin-peroxidase complex. Where the bacterial antigen was localized by ABPAP, a dark brown deposit occurred in the cytoplasms of macrophages and Langerhans' giant cells of the granulomatous lesions. The method approved to be highly specific for the identification of the bacteria and allowed a precise localization of the bacterial antigen in infected cells.