돼지 써코바이러스 2형의 진단을 위한 PCR법 적용 = Application of PCR for diagnosis of porcine circovirus type 2

다국어 초록 (Multilingual Abstract)

Porcine circovirus (PCV) is a small, nonenveloped virus that contains a single-stranded circular DNA genome of about 1.76kb and belongs to the family circoviridae. The PCV-2 has been incriminated as the cause of post-weaning multisystemic wasting syndrome(PMWS), an emerging disease in pigs. In the present study, a PCR assay was applied to detect PCV-2 in tissue samples. The presence of PCV-2 antigen in the porcine tissues was confirmed by indirect immunofluorescence(IIF) with PCV-2 specific monoclonal antibodies. And then DNA extracted from PCV-2 positive tissues was used as a template. One oligonucleotide primer suitable for PCR was selected from a published PCV-2 sequence(Genbank). Amplified PCR product was detected the same fragment lengths of 416bp as a control. Based on these results, it was suggested that the PCR is a simple and sensitive method for support diagnostic purposes.