단호박을 첨가한 마요네즈의 품질 특성

김기쁨,장진아,고준영,최수근 동아시아식생활학회 2009 동아시아식생활학회 학술발표대회논문집 Vol.2009 No.11

슈퍼자미의 이화학적 특성 및 항산화 활성

김기쁨,최수근,김동석,Kim, Ki-Bbeum,Choi, Soo-Keun,Kim, Dong-Seok 한국식품조리과학회 2012 한국식품조리과학회지 Vol.28 No.6

This study attempted to investigate the characteristics of Superjami, which had a high C3G (cyandin-3-glucoside) content by comparing it with common rice (Ilpum) and black rice (Heougjinju, Suwon 415) for their components and physio-chemical characteristics. There were no significant differences in the water content, however there were significant differences in crude protein and crude fat in the order of Ilpum < Superjami < Heougjinju. As for the morphological characteristics of grains, it had been learned that Superjami was heavier and longer than the common rice, moreover Superjami had bigger seeds. As for the hardness of grains, the longer the time of steeping, the more decreased their hardness was at large. In the case of color, they showed significant differences in all the L, a, and b values of grains and flours. As a result of the experiment of pigment elution after steeping of the rice flours at $20^{\circ}C$ and $40^{\circ}C$, the longer the time steeping, the more increased the pigment elution became. The values are bigger with Heougjinju and Superjami than with Ilpum. As for the elution pH after rice steeping, the longer the time of steeping, the lower pH all the assays tended to have. As a result of the analysis of the total polyphenol contents of Ilpum, Heougjinju, and Superjami, it had turned out that the total polyphenol contents of Heougjinju and Superjami are 1.2 times as high as Ilpum, a common rice, and that Superjami is significantly higher than Heougjinju. As a result of the experiment of DPPH radical scavenging ability, there are significant differences among the assays in the order of Superjami > Heougjinju > Ilpum. Futher, it has turned out that Superjami has a higher DPPH radical scavenging ability than Heougjinju. Consequently, it can be stated that Superjami has a strong anti-oxidative ability. Thus, we should more precisely grasp the cooking characteristics of Superjami, which is in the state of brown rice, via comparing it with a common brown rice, and also provide opportunities to apply Superjamii to more foods by studying its starch characteristics in addition to its grain and flour properties.

한국 성인 여성의 연령별 얼굴 노화 정도에 대한 인식과 임상학적 특성에 관한 연구

김기쁨,김지예,안성관,민은설,배승희 한국피부과학연구원 2019 아시안뷰티화장품학술지 Vol.17 No.3

Purpose: In this study, we tried to reveal the levels of the perception and clinical characteristics of facial skin aging among adult Korean women according to age. Methods: Using literature reviews, we determined the different levels of each facial skin aging sign is related to the chronological age of individuals, and developed the multiple-choice chart based on the four different levels; low, medium, high and very high. Forthy Korean participants were asked to describe their perceived levels of the aged signs using the chart. Also, clinical study was conducted to investigate the changes of the aged signs according to age-group among the participants. Results: We found that age-related structural changes on a human face were shown on the ten different regions, including wrinkles in the outer corner and bottom of eyes, wrinkles between the eyebrows, nasolabial lines, pigmentation and skin texture on cheeks, lip lines, sagging on a double chin, pore sizes on the nose, dark circles under the eyes. The survey results showed that the aged levels of all 10 categories were described to be increased as participant-age increases. To verify the results, we clinically tested whether the facial skin aging signs have a tendency to increase with the chronological age using an ANTERA-3D device, and found that the aged levels of the signs were specifically increased with the chronological age. Conclusion: Therefore, our results suggested that the perceived and clinical levels on the 10 regions of facial skin aging were relate to the chronological age. 목적: 본 논문에서는 한국인 여성들을 대상으로 연령별로 나타나는 얼굴 노화 정도에 대한 인식과 임상학적 특성을 밝히고자 하였다. 방법: 참고문헌 조사를 통해, 얼굴에서 나타나는 노화의 세부 부분 및 정도를 결정하였다. 이를 기반으로, 4지선다 형식의 설문지를 개발하였으며, 각 부분에서 나타나는 노화의 정도를 4수준(낮음, 중간, 높음, 아주 높음)으로 구분하게 하였다. 40명의 한국인여성을 대상으로 본인이 가지는 얼굴에 대한 노화 인식 정도를 설문지를 통해 측정하였다. 또한, 얼굴 노화 인식과 실제 연령별 피부 노화 정도와의 관계성을 알아보기 위해, 임상학적 측정을 통해 관찰하였다. 결과: 참고문헌 조사를 통해 나이가 증가함에 딸 얼굴에 나타나는 노화는 세부적으로 10부분(눈 밑 주름, 눈꼬리 주름, 미간 주름, 팔자 주름, 볼 부위 기미, 볼 부위 피부톤, 입술 주름, 이중턱 부위의 피부 처짐, 눈 밑 다크서클, 코 옆 부위의 모공 크기) 에서 나타남을 알았다. 설문조사를 통한 얼굴 노화 정도에 대한인식을 평가한 결과, 10부분 모든 항목 내 노화의 정도와 평가자의 연령대가 비례적인 관계로 나타났다. 이러한 결과를 바탕으로, 얼굴의 노화 정도와 실제 나이와의 연관성을 임상학적으로 증명하고자, 평가자의 얼굴 내 노화 정도를 ANTERA-3D 측정기기를이용하여 측정하였으며, 연령대가 증가할 수록 얼굴 내 세부 노화 정도도 비례적으로 증가함을 보였다. 결론: 따라서, 본 연구 결과는 노화가 진행되면서 나타나는 얼굴 내 세부적인 10부분에 대한 노화 정도 인식 및 임상학적 특성은 연령에 따라 증가함을 나타낸다.

시판 육수 제품의 구매행태 및 만족도 연구

김기쁨 (사)한국조리학회 2023 한국조리학회지 Vol.29 No.5

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국내 다소비 조미류 함유 수용성 비타민 B5의 함량 평가

김기쁨,김민지,안경근,김경하,강련웅,황영선,최용민,조영숙,김행란,정명근 한국식품영양과학회 2014 한국식품영양과학회 학술대회발표집 Vol.2014 No.10

국내 다소비 국에 함유된 수용성 비타민 B<SUB>12</SUB>의 함량 평가

김기쁨,김민지,안경근,김경하,강련웅,이홍헌,황영선,정명근 한국식품영양과학회 2015 한국식품영양과학회 학술대회발표집 Vol.2015 No.8

우리나라 지역별 다소비 생선구이에 함유된 수용성 비타민 B₂의 함량 평가

김기쁨,김민지,안경근,김경하,강련웅,이홍헌,황영선,정명근 한국식품영양과학회 2015 한국식품영양과학회 학술대회발표집 Vol.2015 No.8

고속액체크로마토그래피를 이용한 비타민 B5 및 B6의 정량 분석

김기쁨,황영선,정명근 한국식품영양과학회 2017 한국식품영양과학회지 Vol.46 No.10

식품 함유 비타민 B5 및 B6의 최적 HPLC 분석 조건을 검토한 결과 비타민 B5의 경우 YMC-Pack ODS-AM(250×4.6 mm I.D.) 칼럼을 이용하고, A용매로 50 mM KH2PO4(pH 3.5)을, B용매는 아세토니트릴을 이동상 용매로 사용하는 A용매 95% 등용매용리 조건에서 200 nm의 파장으로 분석하는 HPLC/DAD법을 최적조건으로 확립하였다. 한편 비타민 B6의 최적분석조건은 여기파장(excitation) 290 nm, 방출파장(emission) 396 nm로 분석하는 HPLC/FLD법으로써, 칼럼은 YMC-Pack Pro RS C18(250×4.6 mm I.D.), 이동상 용매는 A용매 20 mM CH3CO2Na(pH 3.6), B용매 아세토니트릴을 A용매 97% 등용매용리 조건으로 사용하였다. 비타민 B5 및 B6의 표준검량선은 R2값이 각각 0.9998 및 0.9999로 고도의 직선성을 나타내었고, 검출한계 및 정량한계는 비타민 B5의 경우 각각 0.4 mg/L 및 1.3 mg/L, 비타민 B6의 경우 각각 0.006 mg/L 및 0.02 mg/L로 산출되었다. Recently, many people have demanded reliable nutritional data even for minor-components. On the other hand, an analytical method for the analyses of vitamin B5 and B6 is lacking. Therefore, this study attempted to validate with accuracy and precision the analysis of vitamin B5 and B6 using a high-performance liquid chromatography (HPLC) method. The vitamin B5 and B6 contents were analyzed using an Agilent 1260 series HPLC system. YMC-Pack ODS-AM (250×4.6 mm I.D.) and YMC-Pack Pro RS C18 (250×4.6 mm I.D.) columns were used for the analyses of vitamin B5 and B6, respectively. In the case of vitamin B5, the flow rate was set to 1.0 mL/min by isocratic elution using the 50 mM KH2PO4 solution (pH 3.5)/acetonitrile (ACN) (95:5, v/v) with monitoring at 200 nm using HPLC/DAD, whereas the flow rate for vitamin B6 was set to 1.0 mL/min of flow rate by isocratic elution using a 20 mM CH3CO2Na solution (pH 3.6)/ACN (97:3, v/v) with monitoring by excitation at 290 nm and emission at 396 nm using HPLC/FLD. The column temperature was set to 30°C. The injection volume was 20 μL for each experiment. The specificity of the accuracy and precision for vitamin B5 and B6 were also validated by HPLC. The results showed high linearity in the calibration curve for vitamin B5 (R2=0.9998**), the limit of detection (LOD) and limit of quantitation (LOQ) were 0.4 mg/L and 1.3 mg/L, respectively, In contrast, for the calibration curve of vitamin B6, which showed high linearity (R2=0.9999**), the LOD and LOQ were 0.006 mg/L and 0.02 mg/L, respectively.

Chlorella vulgaris 미세조류 추출물의 자외선B 의존적 인간 진피섬유아 세포 손상에 대한 보호 효능 연구

김기쁨,이정주,허진아,조대현,김희식,김광년,김수영,안규중,안인숙,안성관,배승희 한국피부과학연구원 2014 대한피부미용학회지 Vol.12 No.4

Ultraviolet B (UVB) radiation accelerates skin aging by inducing direct DNA damage and indirect intracellulardamage, which induce cell cycle arrest and apoptosis in human dermal fibroblasts (NHDFs). In this study,we demonstrated that pretreatment with the hexane extract of Chlorella vulgaris microalgae protects UVBmediatedcell damages in NHDFs. Firstly, we compared the cytotoxicity effect of four extracts (ethanol,hexane, ethyl acetate and distilled water) of Chlorella vulgaris on NHDFs, and found that the four extractshad a little cytotoxicity at 1-20 μg/ml. Additional biochemical assay showed that UVB-induced cytotoxicitywas significantly reduced in NHDFs posttreated with only hexane extract of Chlorella vulgaris . Furthermore,to increase the UVB-protection effect of the extract, we changed the concentration and treatment time, andfound that 48 h of posttreatment with hexane extract (20 μg/ml) of Chlorella vulgaris significantly increasedthe cell viability by 34.01±5.86% compared with the viability of UVB-irradiated NHDFs. In addition, we foundthat the hexane extract of Chlorella vulgaris promoted the DNA repair properties in NHDFs, as revealed byluciferase-based DNA repairing assay. Overall, our results indicate that hexane extract of Chlorella vulgarisis implicated as a potential chemopreventive agent through UVB protection effect on NHDFs.

조리방법별 색상 파프리카에 함유된 수용성비타민 B₂의 함량 변화

김기쁨,강련웅,권희주,김명현,황혜지,주하은,황영선,정명근 한국식품영양과학회 2016 한국식품영양과학회 학술대회발표집 Vol.2016 No.10