Streptococcus faecalis var. liquefaciens 의 Plasmid 와 변이주 형질의 연관성

姜國熙,李命基,金榮勳,李春花,朴淵姬 成均館大學校 科學技術硏究所 1986 論文集 Vol.37 No.2

Lac^- and Prt^- variants were selected from Str. faecalis var. liquefaciens SKD-1007 by acrifiavin treatment. Prt^- Lac^- variant was obtained from Prt^- vaiant by mitomycin C treatment. The plasmid of variants showed same pattern with parent strain. Plasmid cured variants(SKR1, SKR2, SKR3) were obtained from parent treated by novobiocin. SKR3 variant which lost pSK6 plasmid(2.1 Mdal) was sensitive to lincomycin and erythromycin. The results implied that pSK6 plasmid of test strain was associated with antibiotic resistance (L.M, EM).

제약 회귀하의 목표계획법을 이용한 국내 천연가스 산업의 규모와 경제성 분석 : Economies of Scale for the Korean Natural Gas Industry

김봉진,윤희천,이정동,김태유 한국경영과학회 1997 經營 科學 Vol.14 No.1

We consider a problem of estimating the economies of scale for the natural gas industries. The goal programming/constrained regression is employed for estimating the economies of scale for the natural gas industry, and the problem is formulated as a linear programming problem. Also, the translog cost function is used to represent the cost structure for the natural gas industry. The Korean Gas Corporation was selected as a case study, and we demonstrate that the suggested goal programming/constrained regression approach is appropriate for estimating the economies of scale for the Korean natural gas industry.

건설공사의 재해예방에 관한 연구

연길환,조희제,임경택,윤석천 忠南大學校 産業技術硏究所 1994 산업기술연구논문집 Vol.9 No.2

This study aims to review the problems and status of accident and construction projects also to find out the ways of preventing the works from accident. It can let them understand the reasons for the accidents and the way of promoting the awareness of safety. For the study the literature and previous studies are reviewed and then the field managers and safety management members were interviewed intent of status of field safety management and accidents data occurred at domestic construction fields.

인천지역 TDI 노출 작업자에서 직업성천식 유병율

김형렬,김철우,홍윤철,전형준,김치년,김현수,이지나,신주연,고동희,노재훈 大韓産業醫學會 2004 대한직업환경의학회지 Vol.16 No.2

목적 : TDI는 직업성천식을 일으키는 대표적인 물질로 알려져 있다. 과거 TDI에 노출된 작업자들의 천식 발생률을 5~6%가량 보고하였고, 국내에서도 2-20%에 이르는 유병율을 보고한바 있다. 작업환경이 많이 개선되고, 노출량이 감소된 최근에도 TDI에 의한 직업성천식에 대한 보고가 많이 이루어지고 있어, 이에 대한 적극적인 조사와 진단과정을 통해 유병율을 구하고 질병발생의 변화양상을 파악해 보고자 하였다. 방법 : 인천지역에서 특수건강진단을 수행하는 2개 기관의 TDI 노출 근로자 400여명 중, 사업장의 규모가 50인 이상이며 사업주가 연구진행을 허락한 사업장에서 근무하는 근로자 중 직접적으로 TDI에 노출되는 작업에 종사하는 근로자 170명을 연구대상으로 하였다. 이들에 대해 설문지와 산업의학의사의 면담을 통한 작업관련성 평가를 근거로 정밀대상자를 선정하고 이들에 대해 메타콜린 유발시험, TDI 유발시험 등을 수행하여 직업성천식을 확인하였다. 결과 : 설문조사 결과 11명이 직업과 관련하여 천식증상을 보이는 것으로 나타났고(6.9%), 이중 7명에서 메타콜린 유발검사 양성소견을 보였다. TDI에 의한 특이유발시험 결과 1명에서 양성반응이 나타나, 이 집단에서 TDI에 의한 직업성천식의 유병율은 0.58%였다. 결론 : 과거 국내연구에서 TDI에 의한 직업성천식의 유병율이 2~20%에 이르렀던 데 비해, 본 연구에서는 0.58%로 나타났다. 이는 국외 논문에서도 나타나는 경향으로 작업장 노출량 감소로 점차 발생률이 감소하는 양상을 보이고 있다. 그러나 이미 증상이 나타난 근로자들이 부서를 옮기거나, 직장을 그만둠으로 인해 그 유병율이 과소평가되었을 가능성도 있다고 판단된다. Objecdives: This study was carried out to estimate the prevalence of isocyanate-induced occupational asthma in toluene diisocyanate (TDI) exposed workers. Methods: We examined 170 workers who had been directly exposed to TDI through a medical questionnaire, physical examination, and pulmonary function test. Based on screening examination, workers with suspected occupational asthma were selected for further evaluation such as methacholine and TDI challenge tests. Results: Eleven (6.9%) among 170 workers complained of symptoms of occupational asthma. and 7 among these 11 symptomatic workers showed positive responses to the methacholine challenge test (4.1 %). One spray painter was confirmed as having the TDI induced occupational asthma following a positive response to TDI challenge test. Conclusions: The prevalence of TDI-induced asthma was at 0.58% was lower than that for former studies (2-20%). Improved workplace environment, lower level of TDI exposure compared to the past, and the healthy workers effect may have contributed to this low rate of asthma prevalence in workers with TDI exposure.

전류 비유 모델에 의한 초등학생의 전류 개념 변화 분석

유병길,강인석,김병철,남만희,박선희,소하연,윤희정,이영아,이하룡,전병문,정승호 부산교육대학교 과학교육연구소 2000 科學敎育硏究 Vol.25 No.-

There exists a major impediment to learning electricity which may illuminate why direct presentation of scientists' ideas is too aggressive a strategy. This impediment lies in thinking that electricity is used up and in envisaging the light bulb as a consumer. This view is based on a powerful idea which summarises much of our experience; it declares that 'things get used up', 'you cannot get something for nothing', and 'intuitive thinking'. To replace this view with scientific view, we made the analogy model. It is suggested that the analogy model is more effective in teaching electricity circuits than conventional teaching method.

Magnetic bead capture 를 이용한 염색체 띠 7q11, 7q22 에 위치한 cDNA 선별

김영수,김의숙,강윤희,전용혁,김현,박선화 고려대학교 의과대학 1997 고려대 의대 잡지 Vol.34 No.3

Recently, surmountable amounts of genes are being cloned and it has become neccessary to develope new techniques for discovering genes with known chromosomal location and their possible functions. We have developed one such a method and applied it to search for genes that may play a functional role during the development of the central nervous system. To observe the distribution of 18 weeks old fetal brain cDNAs on the chromosome, fluorescent in situ hybridization (FISH) was performed with biotin labeled 18 weeks old fetal brain cDNAs. Among the chromosome bands showing strong hybridization with the cDNAs, 7q11 and 7q22 were microdissected and amplified by PCR. Each band-specific probe pool was confirmed by FISH and band specific cDNAs were selected by magnetic bead capture method. Selected cDNAs were subcloned to plasmid vectors and the nucleic acid sequences were analysed. As a result 41 different clones from 7q11 and 43 different clones from 7q22 were obtained. They were categorized as 25 clones of well characterized genes, 22 clones showing low homology with known genes, 13 clones of simply registered uncharacterized human cDNAs, and 24 clones of unknown genes. From these results, it may be suggested that our technique is very useful to clone the genes expressed in the developing human brain with confirmed chromosomal location. In addition, this cloning technique can be used to discover the new genes related with neural development in combination with functional screening methods.

우리나라 논 관개용 지하수 수질 특성

김진호,조광래,임수정,이경자,경기천,엄미정,김희권,김찬용,이영한,이신찬,윤순강 한국환경농학회 2003 한국환경농학회지 Vol.22 No.4

This study was carried out to evaluate the status of the groundwater quality for paddy fields irrigation in Korea. Water samples were collected at 130 sites throughout the country. Samples were collected at three seasons-April, July and October-in 2000. According to our survey, the groundwater was found to be suitable for irrigation purpose. Average EC was shown 0.286 dS/m. Nitrate-nitrogen and Cl^(-) concentration was 5.6 ㎎/L, 32.95 ㎎/L which satisfied the Korean Standards for Irrigation Water. Nitrate-nitrogen concentration in each province was shown as following orders: Jeju (11.17 ㎎/L) > Chungnam (8.16 ㎎/L) > Gyeongbuk (6.64 ㎎/L) > Gyounggi (5.91 ㎎/L) > Chungnam (4.95 ㎎/L) > Gyeongnam (3.91 mgk) > Jeonbuk (3.50 ㎎/L) > Jeonnam (3.27 ㎎/L) > Gangwon (2.91 ㎎/L). The concentration by sampling seasons were October (6.62 ㎎/L) > July (5.88 mg&) > April (4.78㎎/L). As the soil of Jeju Province is usually derived from volcanic ash soils mainly used for upland drops, it may influence the nitrate-nitrogen concentration of groundwater. The amount of rainfall also influence the water quality. But the COD_(Cr), were shown April (3.17㎎/L) > July (2.91 ㎎/L) > October (2.40 ㎎/L), it is highly related in the basal dose of organic matter fertilizers. This study demonstrated that groundwater quality was suitable for irrigation, but continuous monitoring is recommended for agricultural policy and developing OECD agricultural environment indicators.

조혈모세포이식 후 발생한 주폐포자층 폐렴에 대한 고찰

주지현,최정현,이동건,백지연,고윤호,이혜정,김세희,신호진,박윤희,박지영,김유진,신완식,김춘추 대한감염학회 2001 감염 Vol.33 No.4

Background : Pneumocytitis cainii pneumonia (PCP) can occur in immunocompromised hosts especially such as AIDS or cancer patients. Although recent research had focused on PCP in AIDS patients, few studies have described the clinical presentations of PCP in recipients of stem cell transplantation (SCT). We evaluated the clinical manifestations of PCP in SCT patients admitted at St. Mary's hospital, Seoul, Korea. Methods : The medical records of 17 PCP patients undergoing SCT between Feb. 1998 and Feb. 2000 were reviewed. The diagnosis of PCP was confirmed through the demonstration of Pneumocytitis cainii via either cytology of brochoalveolar lavage (BAL) or histological technique of lung biopsy. CMV disease and CMV infection were confirmed by BAL culture and antigenemia respectively . Results : Seventeen patients were all recipients of allogeneic SCT and 7 of 17 patients were performed non-sibling SCT. Patients presented with symptoms including brief period (4 ∼23 days) of fever (76%), dyspnea (70%), cough (64%), and signs such as rare(58.8%), Sixteen patients (94%) had been receiving immunosuppressive agent such as cyclosporine A (64%) or Fk506 (35%) without PCP prophylaxis. Eleven patients (64%) were treated with corticosteroid with mean dose of 16 mg/day prednisolone and mean duration of 4.6 months after post-SCT period. Twelve patients were co-infected with CMV. Another co-infected miCroorganisms were Pseudomonas aeruginosa, Mycobacterium tuberculosis, herpes simplex virus, parainfluenza virus, Average duration of treatment with trimethoprim-sulfamethoxazole (TMP/SMX) was 21 ±9 days. Four patients died, and three of them were related with PCP. Conclusion : PCP developed frequently in patients who were taking immunosuppressive drug due to graft versus host disease or were not taking TMP/SMX prophylaxis. High risk patients showing fever, cough, or dyspnea should be considered to take early bronchoscopic intervention for detection of PCP. When treat for PCP, it also be considered to the possibility of coinfection such as CMV. (Korean J Infect Dis 33:273∼279, 2001)

Clematis chinensis suppresses lipopolysaccharide-induced expressions of inducible nitric oxide synthase and cyclooxygenase-2 in mouse BV2 microglial cells

Chun, Hae-Jin,Lee, Choong-Yeol,Lee, Jin-Woo,Sung, Yun-Hee,Kim, Sung-Eun,Kim, Young-Sick,Shin, Mal-Soon,Kim, Chang-Ju,Lee, Hye-Jung,Kim, Dong-Hee Kyung Hee Oriental Medicine Research Center 2010 Oriental pharmacy and experimental medicine Vol.10 No.3

Clematis chinensis is the root of Clematis chinensis OSBECK and is classified in Ranunculaceae. Clematis chinensis is a traditional medicinal herb possesses analgesic, diuretic, anti-tumorigenic, and anti-inflammatory effects. In this study, the effect of aqueous extract of Clematis chinensis against lipopolysaccharide-induced inflammation was investigated in mouse BV2 microglial cells. The aqueous extract of Clematis chinensis at the respective concentration was treated one hour before the lipopolysaccharide treatment in mouse BV2 microglial cells. From the present results, pre-treatment with the aqueous extract of Clematis chinensis suppressed prostaglandin E2 synthesis and nitric oxide production by inhibiting on the lipopolysaccharide-stimulated cyclooxygenase-2 and inducible nitric oxide synthase expressions in mouse BV2 microglial cells. The present study suggests that Clematis chinensis may offer a valuable mean of therapy for brain inflammatory diseases.

국내 유통 식품의 잔류동물용의약품 모니터링

김희연(Hee Yun Kim),정소영(So Young Chung),최선희(Sun Hee Choi),이진숙(Jin Sook Lee),최인선(In Sun Choi),조민자(Min Ja Cho),신민수(Min Su Shin),송재상(Jae Sang Song),최재천(Jae Chun Choi),박희옥(Hee-Ok Park),하상철(Sang-Chul Ha),신일식( 한국식품과학회 2010 한국식품과학회지 Vol.42 No.6

알(卵)류 및 축·수산물 321건에 대하여 엔로플록사신, 시프로플록사신, 노르플록사신, 아목시실린, 암피실린, 옥소린산 및 옥시테트라싸이클린, 테트라싸이클린, 클로르테트라싸이클린 및 독시싸이클린에 대한 모니터링을 실시한 결과 총 300건(93.5%)은 조사대상 동물용의약품이 불검출되었으며 검출된 21건(6.5%)의 시료 대부분은 현재 식품공전의 잔류허용기준 이하로 안전한 것으로 나타났다. This study was conducted to monitor residues of 10 veterinary drugs in food products. Various veterinary drugs were examined including enrofloxacin, ciprofloxacin, norfloxacin, oxolinic acid, amoxicillin, ampicillin, oxytetracycline, tetracycline, chlortetracycline, and doxycycline in beef, pork, egg, chicken, eel, flatfish, and rockfish obtained from 6 different regions (Seoul, Incheon, Daejeon, Gwangju, Daegu, Busan). Residues were detected in 21 (6.5%) samples out of 321 samples. In particular, 2 (1.0%) livestock samples had detected residues among 203 products, and 19 (16.1%) aquaculture samples had residues detected among 118 products. The most frequently detected drug residues in aquaculture products were oxytetracycline and amoxicillin, but the levels were mostly below the MRL (Maximum Residue Limit). In only one flatfish sample, amoxicillin was found at a level higher than the MRL (0.05 mg/kg). In livestock products, residues of most veterinary drugs were not detected. But enrofloxacin was detected in 2 chicken (Korean name: Ogolgae) samples at a higher level than the MRL (0.1 mg/kg as the sum of ciprofloxacin).