정상면역을 가진 성인에서 발생한 식도 방선균증

김현수 ( Hyun Soo Kim ),천종운 ( Jong Woon Cheon ),김민수 ( Min Su Kim ),정창길 ( Chang Kil Jung ),김경록 ( Kyung Rok Kim ),최재원 ( Jae Won Choi ),강동우 ( Dong Woo Kang ),김선영 ( Sun Young Kim ) 대한소화기학회 2013 대한소화기학회지 Vol.61 No.2

Hyun Soo Kim, Jong Woon Cheon, Min Su Kim, Chang Kil Jung, Kyung Rok Kim, Jae Won Choi, Dong Woo Kang and Sun Young Kim1 Departments of Internal Medicine and Pathology1, DongKang Medical Center, Ulsan, Korea Actinomycosis is a chronic suppurative disease and caused by Actinomycosis species, principally Actinomyces israelii, which are part of the normal inhabitant on the mucous membrane of the oropharynx, gastrointestinal tract, and urogenital tract. It usually affects cervicofacial, thoracic and abdominal tissue. Cervicofacial type has the highest percentage of occurrence with 50%. Actinomycosis frequently occurs following dental extraction, jaw surgery, chronic infection or poor oral hygiene. It may also be considered as an opportunistic infection in immunocompromised patients such as malignancy, human immunodeficiency virus infection, diabetes mellitus, steroid usage or alcoholism. But, actinomycosis rarely occurs in adults with normal immunity and rare in the esophagus. We report an unusual case of esophageal actinomycosis which was developed in a patient with normal immunity and improved by therapy with intravenous penicillin G followed oral amoxicillin, and we also reviewed the associated literature.

Development of a noninvasive KIM-1-based live-imaging technique in the context of a drug-induced kidney-injury mouse model

Tae-Jun Kwon,Da-Sol Lee,Md. Enamul Haque,Rang-Woon Park,Byungheon Lee,Dongkyu Kim,Yong-Hyun Jeon,Kil-Soo Kim,Sang Kyoon Kim 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

The development of reliable methods to diagnose acute kidney injury is essential to allow the adoption of early therapeutic interventions and evaluate their effectiveness. Based on the fact that kidney injury molecule-1 (KIM-1) expression levels in kidneys are markedly upregulated early after a damage event, here we developed a noninvasive KIM- 1-based molecular imaging technique to detect kidney injury. First, we took advantage of a phage-display platform to select small peptides demonstrating a specific high binding affinity to KIM-1. The promising candidate was conjugated with fluorescent probes, and its imaging potential was validated in vitro and in vivo. This peptide, with the sequence CNRRRA, not only showed a high imaging potential in vitro, allowing a strong detection of KIM-1 expressing cells by microscopy and flow cytometry but also generated a strong kidney-specific signal in live-imaging in vivo experiments in the context of a drug-induced kidney-injury mouse model. Our data overall suggest that the CNRRRA peptide is a promising probe to use in the context of in vivo imaging for the detection of KIM-1 overexpression in damaged kidneys.

HMG-CoA 환원효소 억제제에 의한 ICAM-1 유전자의 발현조절

김현진,정효균,홍우정,김군순,조영석,김도희,채수흥,구본정,송민호,노흥규,김영건 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1

Background : ICAM-1 act as one of major adhesion molecules in the atherosclerotic lesion. ICAM-1 expression is mainly regulated at the level of transcription and depend on IFN-γ signal transduction pathway in which the STAT1 transcrption factor is a critical intermediate. IFN-γreceptor not only initiates tyrosine 701 phosphorylation of STAT1 by Jak1 and Jak2, but also phosphorylates serine 727 through the activation of Raf-1/MAP kinases. HMG-CoA reductase inhibitors have anti-atherosclertic effects, beyond normalization of hypercholesterolemia, by directly acting on endothelial cells, macrophages and vascular smooth muscle cells. HMG-CoA reductase inhibitors suppress the synthesis of isoprenoid intermediates such as geranylgeranyl-pyrophosphate or farnesylpyrophosphate. These effects results inhibitors suppress the synthesis of isoprenoid intermediates such as geranylgeranyl-pyrophosphate or farnesylpyrophosphate. These effects results inhibition posttranslational farnesylation and geranyl-geranylation processing of small GTP-binding preoteins and inhibition of normal signaling activities. Method : We made several 5'-deletion constructs of rat ICAM-1 promoter and analyzed the promoter activities by measuring the luciferase activity after transfection into ECV304 cells and smooth muscle cells. We checked the level of total and phosphorylated STAT1 protein by immunoblot analysis using specific antibodies. Results : Lovastatin inhibits IFN-γ-induced ICAM-1 gene expression in the ECV304cell. The cells pretreated with PD98059, MEKK inhibitor showed significantly low ICAM-1 RNA induction with IFN-γ stimulatio. IFN-γ induced phosphorylation of tyrosine 701 was not significantly changed by the pretreatment of lovastatin. But lovastatin suppresses IFN-γ-induced phosphorylation of ERK1/ERK2 which are responsible for the seine 727 phosphorylation in STAT1. Conclusion : We showed that HMG-CoA reductase inhibitors, lovastatin, suppresses IFN-γ mediated ICAM-1 gene expression through the inhibition of transcription. HMG-CoA reductase inhibitor suppresses IFN-γ-induced phosphorylation of serine 727 in STAT1 through the modulation of MAP kinases.

2003년도 해양스포츠 체험활동을 통한 소아건강교실 사례 발표

김현준,이상엽,이가영,곽현,이남수,김혜선,김윤진,김인주,김용기,대한비만학회 부산분과학회 일동 대한비만학회 2003 The Korean journal of obesity Vol.13 No.4

부산에 소재한 3개 초등학교 청소년단체 소속의 초등하교 3학년부터 6학년까지 구성된 172명을 대상으로 청소년단체 지도자, 초등학교교사, 자원봉사자, 의사와 영양사가 1박 2일간의 해양스포츠 체험활동 을 통한 건강교실을 시행하였다. 대상자의 체질량지수, 체지방량을 측정하여 비만정도를 평가하였으며, 칼로리 섭취에 대한 강의를 시행하였고, 설문지 조사를 통해 대상자의 식이 섭취 습관에 대한 조사를 시행하였고, 본 건강교실에 대한 만족도를 측정하였다. 대상자중 남자에서는 40%, 여자에서는 25.69%에서 비만 경향을 보였다. 대상자의 대부분에서 시행된 건강교실에 긍정적 반응을 보였으며, 향후 체계적인 구성과 철저한 사전계획이 병행되어 본 증례 보고와 같은 건강교실을 시행한다면, 비만아동에서 거부감 없이 비만에 대한 교육을 실시할 수 있으며, 정상 체중 아동군에서도 비만에 대한 예방교육을 시행할 수 있는 프로그램이 될 것이다. 172 elementary school students involved this health program. This subjects were learened about obesity that affect health problem, ideal eating habits, calories that contained in the children's favorite foods by doctors and nutritionist. And this stubjects were checked heights, weight, body fat percent. Obesity is 40% in the boys and 25.69% in the girls. Most subjects were satisfactory with this health program. So we will effectively educate obese children without resistance and prevent normal weight children from obesity through structured and well-designed health program that similar to this program.

항암제 처리한 백혈병 세포주에서의 Apoptosis 발현

김삼용,윤소현,김현수,김종숙,윤환중,김진경,조덕연 충남대학교 암연구소 1998 癌共同硏究所 硏究誌 Vol.2 No.1

The bcl-2 proto-oncogene encodes a 26 kD protein that promotes cell survival by blocking apoptosis. The bax protein is a member of the bcl-2 familly, now known to form heterodimers with the bcl-2 protein. The ratio of bax to bcl-2 is be critical in determining the fate of the cell in response to stimuli that can induce apoptosis. Extract of Pulsatilla Koreana (SB-31) showed promising antitumor activity in vitro with Topo I inhibitory action. In the present study, the relationship between apoptosis and the apoptosis related proteins, bcl-2 and bax were investigated in human leukemic cell lines HL-60, U-937 and CEM-CM3. All anticancer drugs(adriamycin, etoposide, camptothecin, SB-31) induced extensive apoptosis in HL-60, U-937 cells and CEM-CM3 cells. The expression of bcl-2 and bax protein were determined in cell lines by western blotting before and after incubation with anticancer drugs at different time points. 1) In HL-60 or U-937 cell lines, down regulation of bcl-2 and up-regulation of bax were found after incubation with ADR, VP-16 or camptothecin. 2) In HL-60 or U-937 cell lines, no significant change in bcl-2 or bax protein expression resulted after incubation with SB-31. 3) In CEM-CM3 cells, virtually no change was noted in bcl-2/bax expression after incubation with ADR, VP-16, camptothecin or SB-31. It is suggested that different leukemic cell lines use different pathways of apoptosis activation and a given cell may utilize different pathways of apoptosis activation in response to different cytotoxic agents.

클럽 샤프트(Club Shaft) 특성에 따른 골프 스윙(Golf Swing)동작 분석

김성일,김기형,김형수,이현섭,김진욱,안찬규,김희진 한국운동역학회 2002 한국운동역학회지 Vol.12 No.2

본 연구는 클럽 샤프트의 대표적인 재질인 그라파이트(graphite)의 유연한(flexible) 클럽 샤프트(club shaft)의 특성에 따라 피험자가 운동학적(kinematics) 요인이 되는 관절의 각변위, 각속도, 각가속도, 클럽헤드의 속도와 가속도와 같은 변인들이 어떻게 적응하는지 비교 분석하여 보다 효율적인 드라이버 선택에 도움을 주며 샤프트 특성에 따른 신체관절의 움직임에 대한 자료를 제시하고자 하였다. 고속 카메라 2대의 속도는 500fps로 하였고 각 regylar,stiff, x-stiff, 자신의 클럽을 포함 각 4개의 클럽을 사용하여 각 클럽당 3번씩 촬영하였으며 목표방향에서 20m이상 벗어나는 경우의 촬영은 다시 촬영하였다. 본 연구에서는 디지타이징(digitizing)을 신체 9개 마커는 강체로 가정된 클럽과 신체분절 모델로 정의하였으며 2 대의 카메라(500fps)로부터 얻은 avi화일을 컴퓨터에 저장하고 자료로부터 Butterworth 6th order recursive digital filter를 사용하여 1차 자료를 smoothing 하고 DLT를 이용하여 3차원 좌표를 구성하도록 한다. 좌표값을 얻기 위하여 kwon3d v3.0을 이용하였다. 본 실험은 피험자 스스로 클럽의 특성에 따라 스윙의 속도를 달리 하기 때문에 스윙의 시간이 달라지며 어느 정도 클럽이 강성에 따라 스윙시간이 빨라지는 결과로 나타났다. 이것은 피험자가 샤프트가 강성(stiffness)에 따라 스윙 속도를 빨리 하게 되는 원인이 되는 것으로 생각된다. 어깨의 각변위는 클럽이 regular의 경우 임팩트에서 각속도를 계속 유지하고 있으며 stiff, x-stiff의 경우에는 어깨의 움직임이 임팩트에서 급격하게 감소되는 것을 알 수 있다. 이것은 팔의 동작과 클럽의 힘을 크게 하기 위한 동작으로 생각된다. 어깨 각속도는 클럽이 stiff할수록 각속도가 큰 감속하는 것으로 나타났다. 손목속도는 regular 클럽의 경우 손목의 감속이 늦게 되고 임팩트에서 손목의 감속이 적게 하는 것으로 나타났으며 stiff와 x-stiff의 클럽에서 임팩트 시에 순간적인 감가속으로 인해 클럽의 속도를 증가시키고 있다. 임팩트 시에 손목의 감가속은 클럽헤드의 임팩트 시 속도를 증가시키는 결과를 보였다. 클럽헤드는 regular 클럽이 임팩트전에는 속도 증가가 커지는 결과와 일치된 결과를 보이고 있다. The purpose of this study was to find the rational method to analyze golf swing with specific property of club shaft. Three subjects were filmed by two high speed digital cameras with 500fps. The phase analyzed was downswing of each subject. The three-dimensional coordinates of the anatomical landmarks were obtained with motion analysis system Kwon3d 3.0 version and smoothed by lowpass digital filter with cutoff frequency 6Hz. From these data, kinematic and kinetic variables were calculated using Matlab(ver 5.0) The variables for this study were angular velocity and accelerations, which were calculated and following conclusions have been made : 1) Golf swing time of stiff club is faster than that of regular club. 2) In shoulder joint motion of swing with the stiff club, x-stiff showed mort rapid negative acceleration than that of regular club. 3) In regular club, the velocity of club head would be more effective velocity, which was increasing, than those of other clubs before impact. 4) In wrist joint motion of swing with stiff club, x-stiff club showed faster than regular club in the downswing and impact more rapid negative acceleration.

해조류 용매별 추출물의 항돌연변이원성

김봉오,박제석,오명철,현재석,김재하,오창경,김수현 濟州大學校工科大學産業技術硏究所 1996 尖端技術硏究所論文集 Vol.7 No.2

톳, 미역 및 다시마를 용매별로 추출하여 이들 추출물의 항돌연변이원성을 검색하였다. 톳 용매별 추출물의 MMS에 대한 항돌연변이 효과는 TA100 균주에서 헥산, 에탄올, 에칠에테르 추출물 순으로 높았고, TA102 균주에서 헥산, 에칠에테르, 에탄올 순으로 높았으나, 미역 및 다시마 추출물은 항돌연변이원성이 거의 없었다. ICR191에 대한 항돌연변이 효과는 시험된 모든 시료에 대한 에탄을 추출물이 높은 항돌연변이 효과를 나타내었고, 에칠에테르와 헥산 추출물은 물 추출물보다도 낮은 효과를 나타냈다. 그러나 톳 추출물은 시험된 모든 용매 추출물에서 60% 이상의 높은 항돌연변이 원성을 나타내어 ICR-191에 의해 유도되는 돌연변이 원성을 저해하는 물질이 수용성 및 비수용성에 걸쳐 다양함을 나타내었다. 한편, 미역, 다시마 및 톳 추출물의 sodium azide에 대한 항돌연변이원성은 없는 것으로 나타났다. Desmutagenic effects against methyl methanesulfonate(MMS)-, 2-methoxy-6-chloro-9-〔3-[2-chloroethyl〕aminopropylamino〕acridine(ICR191)- and sodium azide-induced mutations by the extracts from Hizikia fusiforme. Laminaria japonica and Undaria pinnatifida with different solvents were investigated. The solvent extracts from Hizikia fusiforme were the highest desmutagenic effect against MMS in n-hexane and against ICR191 in ethanol extract. but no against sodium azide in all solvent extracts tested. While the solvent extracts from Undaria pinnatifida and Laminaria japonica were the highest against ICR191 in ethanol extract. but no against sodium azide and MMS in all solvent extracts tested. Moreover. all the solvent extracts from Laminaria japonica were inhibited 60% or more ICR191-induced mutations.

STS 410 스테인레스강의 피로균열전파특성에 미치는 온도, 주파수 및 응력비의 영향

김현수,김현철,임병수 成均館大學校 科學技術硏究所 1994 論文集 Vol.45 No.1

The effects of temperature, stress ratio and frequency on the fatigue crack propagation have been investigated using STS 410 Stainless Steel. The temperatures employed were room temperature(27℃), 300℃ and 500℃. The stress ratios were 0.1 and 0.3 and the frequencies were 20 and 30Hz. Some of the conclusions obtained are as follows. (1) As the temperature increased, da/dN became greater, however, with the greater stress ratio, this tendency became smaller. (2) The change of frequency to a higher value at a given temperature had little effect on da/ dN. (3) The increase of stress ratio increased da/ dN, however, this effect became smaller at higher temperature.

2003년도 해양스포츠 체험활동을 통한 소아건강교실 사례 발표

김현주,이상엽,이가영,곽현,이남수,김혜선,김윤진,김인주,김용기,대학비만학회 부산분과학회 일동 대한비만학회 2003 The Korean journal of obesity Vol.12 No.4

항암제 처리한 백혈병 세포주에서의 Apoptosis 발현

김삼용,윤소현,김현수,김종숙,윤환중,김진경,조덕연 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

The bcl-2 proto-oncogene encodes a 26 kD protein that promotes cell survival by blocking apoptosis. The bax protein is a member of the bcl-2 familly, now known to form heterodimers with the bcl-2 protein. The ratio of bax to bcl-2 is be critical in determining the fate of the cell in response to stimuli that can induce apoptosis. Extract of Pulsatilla Koreana (SB-31) showed promising antitumor activity in vitro with Topo I inhibitory action. In the present study, the relationship between apoptosis and the apoptosis related proteins, bcl-2 and bax were investigated in human leukemic cell lines HL-60, U-937 and CEM-CM3. All anticancer drugs(adriamycin, etoposide, camptothecin, SB-31) induced extensive apoptosis in HL-60, U-937 cells and CEM-CM3 cells. The expression of bcl-2 and bax protein were determined in cell lines by western blotting before and after incubation with anticancer drugs at different time points. 1) In HL-60 or U-937 cell lines, down regulation of bcl-2 and up-regulation of bax were found after incubation with ADR, VP-16 or camptothecin. 2) In HL-60 or U-937 cell lines, no significant change in bcl-2 or bax protein expression resulted after incubation with SB-31. 3) In CEM-CM3 cells, virtually no change was noted in bcl-2/bax expression after incubation with ADR, VP-16, camptothecin or SB-31. It is suggested that different leukemic cell lines use different pathways of apoptosis activation and a given cell may utilize different pathways of apoptosis activation in response to different cytotoxic agents.