비소세포폐암에서 Microsatellite Instability

전효성,김정란,손지웅,박선하,박태인,김창호,김인산,정태훈,박재용 경북대학교 병원 2001 경북대학교병원의학연구소논문집 Vol.5 No.1

연구베경:MMR 유전자의 불활성화에 의해 야기되는 뮤전적 불안정성은 발암기전의 한 부류로 인정되고 있다.저자들은 비소세포폐암의 발암과정에서의 MSI의 역할을 규명하기 위해 비소세포폐암에서 MSI의 빈도 및 MSI 유무에 따른 임상상의 차이를 조사하였다. 대상 및 방법:근치적 절제술을 받은 비소세포폐암 20예를 대상으로 하였다.동결된 폐암조직과 환자의 림프구에서 DNA를 추출한 후 3P와 9p의 15개의 marker들을 대상으로 PCR을 시행하고 7% polyacrylamide gel에서 전기영동한 후 silver 염색을 시행하였다. 암조직과 림프구 DNA의 PCR product의 band를 비교하여 MSI와 LOH를 판정하였다. 결과:1)대상환자들은 남자 19예,여자 1예였으며 모두 흡연자였고 평균 흠연력은 47 갑년이었다. 폐암의 조직형은 편평상피암 15예, 선암 4예,대세포암 1예였고, 술 후 병리학적 병기는 Ⅰ기 6예,Ⅱ기 5예, ⅢA기 7예,ⅢB기 2예였다. 2)20예 가운데 8예(40%)에서 MSI가 관찰되었으며 3예는 한 개의 marker에서,5예는 2개 이상의 marker에서 MSI가 관찰되었다. 3)LOH는 10예(50%)에서 있었으며,LOH유무에 따른 병가 및 흡연력의 차이가 없었다. 4)분석한 marker의 10% 이상에서 MSI가 관찰된 MSI-L종양은 5예였으며, 대부분의 marker에서 MSI양성인 MSI-H종양은 없었다.MSS종양과 MSI-L종양은 흡연력, 병기, 폐암 조직형 및 LOH 빈도의 유의한 차이가 없었다. 결론:비소세포폐암에서 MSI는 비교적 흔히 관찰되지만 MMR 유전자의 불활성화에 의한 MMP pathway 는 비소세포폐암의 주요 발생기전은 아닐 것으로 생각된다.향 후 비소세포폐암의 발암과정에 있어서 MMP pathway의 역할을 규명하기 위해서는 보다 많은 예를 대상으로 한 연구가 필요할 것으로 생각되며, MSI 발생기전에 관한 추가적인 연구가 필요할 것으로 생각된다. Purpose: Microsatellite instability(MSI) is frequently used as an indicator of microsatellite mutator phenotype (MMP) tumors.MSI has been observed in a percentage of non-small cell lung cancer(NSCLC).However, its role in tumorigenesis of NSCLC remains unknown.The frequency and partten of MSI in NSCLC were evaluted and clinical parameters of MSI-positive tumors with those of MSS(microsatellite stable) tumors were compared. Materials and Methods: Twenty surgically resected NSCLCs were analyzed for 15 microsatellite markers located at chromosomes 3p and 9p.The peripheral blood lymphocytes of patients were used as the source of the normal DNA. Results:1) of 20 cases, 8(40%) demonstrated MSI. 2) Instability was observed more frequently in tri- and tetra-nucleotide repeats that in dinucleotide repeats. In all cases, instability appeared as a shift of individual allelic bands. 3) LOH was observed in 10(50%) of 20 tumors analyzed. 4) Of 20 cases, MSI-H tumor(showing MSI in the majority of markers) was absent. There were 5 MSI-L tumors( showing MSI in a greater than 10% of markers). 5) No significant difference was observed between MSI-L tumors and MSI negative tumors in terms of clinicopathologic features such as pack-year history of smoking, histologic subtype, and (delete) stage of disease.There was also no significant difference in the incidence of LOH in relation to the status of MSI. Conclusion: These data strongly suggest that MSI plays different roles in lung and colon cancer.MMP pathway appears to be far less important in the tumorigenesis of NSCLC, caused mainly by cigarette smoke, with little familial tendency.(tuberculosis and Respiratory Diseases 2000,48:24-32)

실험적 급성 심근경색증 후 좌심실 재성형에 관여하는 간질 조직의 변화에 대한 전환효소억제제의 기전에 대한 연구 : 백서의 非痛璧性 심근경색 후 좌심실 재성형 및 Transforming Grawth Factor β-1의 발현 변화와 이에 대한 레닌 안지오텐신계 차단제의 효과 분석 Effects on Transforming Growth Factor - β-1 Expression

연태진,김석연,김효수,김어진,김소영,정은주,서정욱,오병희 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.9

그람양성구균에 대한 Teicoplanin과 Vancomycin의 시험관내 항균력

최태열,김경숙,전용관,서일혜,김정욱,이웅수,안정열,김홍석,정재용,최효선,김덕언,유진우 대한감염학회 1994 감염 Vol.26 No.1

An increasing frequency of methicillin resistant S. aureus(MRSA), methicillin resistant coagulase negative staphylococci(MRCNS) and Enterococcal infection have been observed in recent years. Teicoplanin is a new glycopeptide antibiotic obstained from the Actinoplanes teicomycetius. The molecular structure and spectrum of antimicrobial activity of teicoplanin is simillar to those of vancomycin, and has been reported to have an excellent in vitro and in vivo effect against various gram-positive infections. Therefore, we evaluated the in vitor susceptibility of gram positive cocci, such as, S. aureus, coagulase negative Staphylococci(CNS), and Enterococci to teicoplanin and vancomycin. The total 253 strains consisted of MSSA(40), MRSA(53), MSCNS(47), MRCNS(48), and Enterococci(65). They were assayed by disc diffusion and agar dilution. During the study, 57% of S. aureus and 49% of CNS showed resistance to methicillin. The inhibitory diameter of teicoplanin was 15-20mm in MSSA, 12-19mm in MRSA, 13-24mm in MSCNS, 11-23mm in MRCNS, and 15-22mm in Enterococci respectively, and showed sensitivity in all but 8 strains(3.2%). The range of the minimum inhibitory concentration (MIC) of teicoplanin to MSSA, MRSA, MSCNS, MRCNS and Enterococci were 9.12-2.0㎍/ml, 0.25-2.0㎍/ml, & 0.25-32㎍/ml, 0.12-1.0㎍/ml respectively. One case of S. haemolyticus was resistant to teicoplanin (32㎍/ml) by the agar dilution method. Eight minor (3.2%) and one major(0.4%) error was observed when the MIC and disk diffusion data were correlated with teicoplanin. As for vancomycin the inhibitory diameter was 17-21mm in MSSA, 15-21mm in MRSA, 18-26mm in MSCNS, 18-25mm in MRCNS, and 16-22mm in Enterococci respectively. The range of the MIC of vancomycin to MSSA, MRSA, MSCNS, MRCNS, and Enterococci were 0.25-1.0㎍/ml, 0.25-4.0㎍/ml, 0.5-2.0㎍/ml and 0.5-2.0㎍/ml respectively. One minor error (0.4%) was seen with the vancomycin disk. The MIC90 of MSSA and MRSA exhibited the same results in teicoplanin (1.0㎍/ml, 1.0㎍/ml), and vancomycin(2.0㎍/ml, 2.0㎍/ml). MSCNS and MRCNS exhibited greater MIC90 with teicoplanin(4.0㎍/ml, 8.0㎍/ml) than vancomycin(2.0㎍/ml, 2.0㎍/ml). Incontrase Enterococci were more susceptible to teicoplanin(0.5㎍/ml) than to vancomucin (2.0㎍/ml). Results from this analysis indicated that both teicoplanin and vancomycin were very excellent for gram positive infections, especially those resistant to methicillin.

구개부에 발생한 다형성선종의 치험례

이창진,김태열,김효언,김희광,전인성,윤규호 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.2

Pleomorphic adenoma is the most common of all salivary gland tumors, constituting over 50 per cent of all cases of tumors, and approximately 90 per cent of all benign salivary gland tumors. Since the term mixed tumor' was introduced by Broca for its dual origin of epithelial and mesenchymal elements, the term plemorphic adenoma suggested by Willis characterizes closely the unusual histologic pattern of the lesion. The parotid gland is the most common site but it may occur in any of the salivary glands. It is somewhat more frequent in women and in the fourth to sixth decades, but they are also relatively common in young adult and have been known to occur in children. Treatment of choice is surgical excision. Adequate surgery with safe margin reduce its recurrence rate. We represent a case of pleomorphic adenoma with literature review in 65-year old male occured in the palate. The lesion was successfully treated by surgical excision.

Korean English Teacher's Disempowerment in English-only Classes

Sung-Sam Hwang,Hyo-Sun Seo,Tae-Young Kim 한국사회언어학회 2010 사회언어학 Vol.18 No.1

Hwang, Sung-Sam, SeD, Hyo-Sun, Kim, Tae-Young. 2010. Korean English Teacher's Disempowerment in English-only Classes: A Case Study Focusing on Korea-specific Cultural Aspects. The Sociolinguistic journal of Korea 18(1). In this case study, we have focused on the disempowerment of a Korean English teacher (KET) in English-only classrooms. Six English as a foreign language (EFL) classrooms in a Korean high school were observed and analyzed by using interpretive discourse analysis. Out of the six classes, three representative classrooms were both video- and audio-taped. The results show that the English-only classes could weaken the KETs' power via three mechanisms: (a) calling the KETs' last name for choral greetings at the beginning of the class, (b) students' group solidarity and peer pressure between classmates, and (c) the students' excessively collaborative approach to peer assistance. It is argued that the exclusive English-only instruction disempowers the KETs' status and dismantles their vocational identity. In order to remedy the current situation, within a scaffolding supported by the eclectic use of first language where necessary for effective class management, KETs are empowered and overcome constraints imposed by the limited L2 oral competence of some non-native English teachers.

TREE POINT GRAPH에 의한 3dB 周波數決定에 관한 硏究

김태효,신위재 慶南大學校 附設 工業技術硏究所 1989 硏究論文集 Vol.7 No.-

본 논문에서는 다극점(multipole) 회로망의 3dB 주파수를 결정하는데, 계산이나 컴퓨터 연산에 의하지 않고 그래프적 표현기법으로 필터회로망을 해석할 수 있음을 보였으며, 이론의 타당성을 확인하기 위해 소프트웨어 ENPORT를 이용 하여 검증하였다. This paper presented that the analysis of filter network using graph represent technique for the determination 3dB frequency of multipole network not by means of calculation or computational operation. It should that using ENPORT software for confirm the result of proposed algorithm.

ECT (European Compression Technic) System 을 사용한 下顎骨 骨折의 治驗例

金晤煥,申曉根,宋采炫,崔秉河,權泰鎬 全北大學校 齒醫學硏究所 1983 전북치대논문집 Vol.1 No.1

The authors in the Dept. of Oral & Maxillo-facial Surgery.J.N.U Hospital could have the following results after treatment of eight mandibular fractures patients with the E.C.T.(European Compression Technic)System. 1. Accurate reduction of the fractured bone. 2. Minimization of the soft tissue injury during operations. 3. No post-operative intermaxillary fixation was necessary. 4. Good recovery of the occlusion.

시차주사열량분석기를 이용한 치과용 접착시멘트의 경화기전 및 반응열에 관한 연구

김철위,이용근,윤태호,김효종,남세진 大韓齒科器材學會 2000 대한치과재료학회지 Vol.27 No.3

The working time of dental cement has a close relation with the setting time, where the setting reaction can be said as the result of the chemical reaction between cement liquid and powder. The purpose of this experiment was to investigate the setting characteristics and setting rate of dental cements by measuring the exothermic heat(Joule/gram) from the setting reaction at specified times using a differential scanning calorimeter (Differential Scanning Calorimeter, DSC 204, TASC 414/3A Controller, Netzche, Germany). Graphs of time-exothermic heat at 37℃ isothermal condition, showing the setting reaction of dental cements were plotted. Three types of luting cements studied were zinc-phosphate cement (ZPC), polycarboxylate cement (PCC), and glass ionomer cement (GIC). The effects of powder-liquid ratio on the setting characteristics of cements were also included. The amount of heat (calory) released per one gram of cement was calculated at each specified time, and was analyzed. ANOVA and Scheff's multiple comparison test (p=0.05) were used for statistical analysis using SPSS/PC+. The setting rate of cement was not constant during the setting reaction, and different setting characteristics were observed depending on the type of cement and powder/liquid ratio. Generally, 70∼90% of the setting reaction, measured by the exothermic heat release, occurred within 5 minutes after mixing. The exothermic heat released by ZPC was very high immediately after mixing, and the duration of the heat release was prolonged compared with other cements. The exothermic heat release of PCC finished most rapidly. It was generally observed that the amount of heat release (rise in temperature) per one gram of cement was highest for ZPC, followed by GIC, and then PCC. However, heat release during the first 2∼5 minutes after mixing was highest for GIC, followed by ZPC and then PCC. According to the Scheff's multiple comparison test, the duration of the total setting reaction did not show any significant difference among the cements(p>0.05), and also among the different power/liquid ratio groups(p>0.05). It was observed that more heat was released with the lower powder/liquid ratio than the higher powder/liquid ratio.

시대에 따른 청소년 망상의 변화

김양태,임효덕 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.2

연구목적: 저자들은 지난 30년 동안 입원한 청소년 정시병 환자 중 망상이 분명하였던 환자를 대상으로, 시대적 변화와 청소년 문화의 변천에 따른 망상의 유형 및 내용의 변화를 알아보고, 청소년 환자에서의 망상의 특징을 알아보고자 본 연구를 시행하였다. 방법: 경북대학교병원에 입원하였던 청소년 정신병 환자 중 망상이 분명하였던 환자를 대상으로 하였다. 1977년 1월 1일부터 1978년 12월 31일 사이에 입원한 환자 39명, 1987년 1월 1일부터 1988년 12월 31일 사이에 입원한 환자 41명, 그리고 1997년 1월 1일부터 1998년 12월 31일 사이에 입원한 환자 44명에 대한 망상의 주제 및 내용을 비교하였다. 결과: 1) 망상을 나타낸 환자들은 70년대말, 80년대말, 90년대말 세 군 모두 남자의 비율이 여자보다 약간 높았고, 18∼21세 사이의 군이 가장 높은 빈도를 보였고, 비종교군의 비율이 종교군보다 높았고, 부모가다 살아있는 군이 가장 많았다. 학력은 고등학교가 가장 많았으나, 90년대말이 되면서 대학교 학력을 가진 비용이 증가하였고, 출생순위는 70년대말과 80년대말에서는 중간이 가장 많았으나, 90년대말에서는 장자가 가장 많았다. 2) 망상의 유형은 시대에 따라 의미있게 증가하거나 감소한 것은 없었고, 가장 높은 출현빈도를 보인 것은 피해망상, 관계망상, 과대망상 순이었다. 망상의 내용은 친구들 주제로 한 망상의 출현빈도가 가장 많았고, 통계학적으로 의미있게 증가하였다. 3) 남녀별 차이점은, 남자의 경우 관계망상의 출현빈도가 의미있게 증가하였고, 친구들 주제로 한 망상의 출현빈도가 의미있게 증가하였고, 초능력을 주제로 한 망상의 출현빈도가 의미있게 감소하였다. 여자의 경우 과대망상의 출현빈도가 의미있게 증가하였고, 초능력을 주제로 한 망상의 출현빈도가 의미있게 증가하였다. 4) 연령별로 보면, 18∼21세 경우는 피해망상의 출현빈도가 의미있게 증가하였고, 친구를 주제로 한망상의 출현빈도가 의미있게 증가하였다. 그리고 12∼147세에서 15∼17세, 18∼21세로 나이가 증가함에 따라 망상의 종류 및 내용에 다양해지고 있었다. 결론: 시대에 따른 청소년 문화의 변천은 청소년 정신병 환자의 망상의 내용에 영향을 미치는 것으로 생각된다.

1급 응급구조사 분야 국가시험의 문제점 및 개선방안

김태민,김효식 제주한라대학 1998 論文集 Vol.22 No.-

It is about time to have overall change and new direction in the area of the National Examination for the First Level Emergency Medical Technicians 1st. E.M.T. since all the health care related examinations including emergency service area are newly in charge of the National Health Personal Licensing Department. Furthermore the great change is expected on the National Examination for the 1st. E.M.T, because of the fact that the ministry of health and social welfare announced a matter of revision in legislation related to the administration of laws and regulations in emergency service, and claimed to have overall reform in the area of the National Examination for 1st. E.M.T. from January 2000. The National Examination for the 1st. E.M.T. should be revised to be a reliable tool to measure abilities, knowledge, skills, attitudes of the 1st. E.M.T. To solve general problems in the matter of subject selection, the degree of difficulty of the test, the style and the number of the test items, revision and management of the items, proper time and place selection for the national examination, and the test for the practicum and physical strength, following suggestions need to be accomplished. (1) Title and relative importance of the subjects of the test should be adjusted to parallel with the tasks of the emergency medical technicians. (2) The test should be based on the text books which are written according to the goals and objectives of the subjects. (3) Overall test item numbers and ratio of the test style need to be balanced considering other healthcarerelated national examinations. (4) The degree of the difficultly and validity of the test need to be evaluated, and test items should be revised, added, and secured. (5) Consideration should be given to the students from the local areas, shortening the period of the test or changing the test place to the local areas. (6) The validity of the tests for the practicum and physical strength needs to be evaluated, and evaluation tool should be revised as well.