더덕의 효율적인 재분화 및 뿌리발달

심주선,조숙녀,손화,김무성,노영덕,김세영,양덕춘 경희대학교식량자원개발연구소 2005 硏究論文集 Vol.24 No.-

더덕은 식용, 관상용, 생약재료로 쓰이는 방향성 식물이다. 본 연구는, 종자의 기내배양을 통하여 식물체 재분화와 뿌리발달을 실험했다. 종자의 발아는 GA_(3) 100 mg·L(-1)처리와 저온처리 시 매우 양호하였다. 더덕의 기내에서 shoot의 형성은 2 mg·L^(-1) BA, 2 mg·L(-1) NAA가 첨가된 MS 기본배지에서 가장 양호하였으며, 줄기의 절편체와 비교하여 잎 절편체에서 재분화가 잘되었다. 또한 뿌리의 발육은 계속적인 계대배양에 의해서 양호하였는데 shoot의 길이를 1 cm 정도 남긴 뿌리를 연속적으로 4회 계대배양에서 뿌리가 주근으로 발육하였으며 길이는 약 3 cm 및 직경은 0.5 cm의 정상적인 뿌리를 얻을 수 있었다. Codonopsis lanceolata is widely used as edibles, ornamental plant and crude drug material. This study was carried out to confirm the ratio of germination and efficient differentiation of shoot and root in vitro. The germination of Codonopsis lanceolata seed was excellent in the medium with GA_(3) 100 mg·L(-1) and chilling treatment. The shoot formation of Codonopsis lanceolata was good at the MS medium with 2 mg·L(-1) BA and 2 mg·L(-1) NAA. According to inoculum part, ratio of re-differentiation was higher from the leaf explant compared with the stem explant. For root development, successive subculture with 1 cm shoot of upper part from root was efficient. Roots of Codonopsis lanceolata cultured in vitro system was grown like normal root with length of 3 cm and thickness of 0.5 cm at the 4 successive subcultures.

항암 화학요법을 받는 유방암 환자의 삶의 질에 관한 연구

심주현,박경숙 성인간호학회 2004 성인간호학회지 Vol.16 No.1

Purpose: This study is a descriptive research study to measure the quality of life of those who suffer from breast cancer and take the chemotherapy. Method: The Subjects were 70 breast cancer patients who took the chemotherapy from September 2 to October 31, 2003. Quality of life was measured by Ferrell's measurements. Result: Quality of life indicators were spiritual domain=6.44, physical domain=5.45, social domain=4.15, and mental domain=3.95. Whole quality of life was 4.68 out of 10 points. The quality of life of those with a practicing religion was significantly higher than those without(F=3.88, P=0.026). Subjects who were less than 2 months post-operation had higher points in the physical domain than those who were more than 2 months post-operation (t=2.76, p=0.007). Subjects who had less than 4 treatments of chemotherapy had higher points in the physical domain than those who had more than 4 treatments of chemotherapy (t=2.03, p=0.046). Conclusion: The results of this study serve as a meaningful source to promote quality of life of breast cancer patients who undergo chemotherapy. The results can also be applied to the development of education programs and counseling materials for chemotherapy patients. Health care strategy can also raise the quality of life of brest cancer patients.

이온교환법에 의한 고분자전해질형 연료전지용 전극의 제조와 특성 고찰

이주성,박수길,오태영,이홍기,심중표 한양대학교 에너지.환경기술연구소 1995 에너지·環境技術論文集 Vol.2 No.-

고분자전해질형 연료전지용 전극의 백금 이용률을 높이기 위해 촉매제조시 이온교환법을 이용하여 백금을 carbon에 담지하고 이에 따른 전지성능변화를 고찰하였다. 전극제조시 Nafion이 첨가되지 않은 경우 이온교환양이 증가함에 따라 전기화학적인 활성이 증가하여 전지성능이 증가하였다. 전극제조시 Nafion이 첨가된 경우, Nafion첨가량이 증가함에 따라 전지성능은 증가하였는 데 Nafin첨가에 의해 이온교환되지 않은 백금이 전기화학적인 활성을 가지게 되어 전지성능은 증가하였다. To improve platinum utilization of PEMFC(Proton exchange membrane fuel cell) electrode, in preparing catalytic powder, platinum was impregnated on carbon by ion exchange method and its cell performance was investigated. When electrode was prepared without adding Nafion solution, as Nafion pre-impregnated amount was increased, electrochemically active area and cell performance was increased. When electrode was prepared with Nafion solution, increase of adding Nafion amount was improvement of cell performance. By adding Nafion at preparation of electrode, non ion-exchange platinum was shown electrochemical activity, cell performance was improved.

MANET을 위한 섹터기반의 확장성 있는 오버레이 멀티캐스트 프로토콜

李柱日,沈永喆 弘益大學校 科學技術硏究所 2005 科學技術硏究論文集 Vol.16 No.-

MANET is the self-configurable network of many mobile nodes connected by wireless links. It has no centralized access point or existing infrastructure so that communications are made as multi-hop fashion. As nodes move around the network the topology is frequently changed. And due to the very innateness of wireless medium, it suffers from high transmission error rate and limited bandwidth. Nevertheless, MANET can be a good basis for onetime applications like military training or urban tele-conference, which require close collaboration. However, this can be true as long as there is a stable multicast protocol. Overlay multicast protocol is able to cope with dynamic topology and is independent on underlying unicast routing protocol. But, most of the MANET overlay multicast protocols are not scalable, and unclear are their criteria for maintaining virtual topology. In this paper, we propose a structured sector-based scalable end-system multicast protocol with clear criteria for maintaining virtual topology.

수면-무호흡 중후군 증례보고

최동주,김영환,심영수,김건열,한용철,유빈,송재훈,한성구 대한내과학회 1987 대한내과학회지 Vol.32 No.4

Sleep apnea syndrome is characterized by loud snoring, excessive daytime somnolence and multiple attack of apnea during sleep which can cause sudden nocturnal death, cor pulmonale, hypertension, personality change and morning headache. We report a typical case of obstructive sleep apnea syndrome in a 30 year old Korean woman. She was very obese and complained loud snoring, daytime hypersomnolence. Nocturnal sleep study showed repetitive attack of apnea and fall of arterial oxygen tension and saturation. Bilateral severe tonsilar hypertrophy was thought to be the mechanism of obstruction.

인삼 Peroxidase(PgPrx3) 유전자의 분리 및 연초의 형질전환

손화,심주선,김세영,노영덕,김무성,양덕춘 경희대학교식량자원개발연구소 2005 硏究論文集 Vol.24 No.-

Peroxidase는 항산화에 관련된 효소이며 식물의 환경스트레스와 성장에 관련되는 중요한 유전자이다. 본 실험에서 사용된 Peroxidase Ⅲ의 cDNA는 Panax ginseng C.A. Meyer 잎에서 추출하였으며 PgPrx3라 명명하였다. 이 gene의 ORF영역은 1,065 bP이고 355개의 아미노산을 가지고 있다. BioEdit 프로그램을 이용하여 PgPrx3와 다른 식물의 peroxidase gene과 비교한 결과 상동성을 가지고 있었으며, Spinacia oleracea(70%), Vigna angulais(71%), Nicotiana tabacum(69%) and Linum usitatissimum(65%). 그중 Vigna angularis의 상동성이 가장 높았다. 담배에 본 유전자를 형질전환시켜 유전자의 특성을 검정하기 위하여 35S/35S/AMV/peroxidas/Tnos를 벡터 pRD400에 재조합 후 아그로박테리아를 이용하여 담배에 도입시켰다. 그리고 PCR를 이용하여 유전자 PgPrx3가 담배에 도입된 것을 확인하였으며, RT-PCR로 정상적으로 PgPrx3 유전자가 전사되어 RNA를 생성하고 있음을 확인하였다. A peroxidase (E.C. 1.11.1.7) is very important enzyme as antioxidants. The function of this gene is connected with growth and environmental stress of plant. A class Ⅲ peroxidase cDNA was isolated from the leaf of Panax ginseng C.A. Meyer and named as PgPrx3 which is consisted of an ORF (open reading frame) of 1,065 bp and an amino acid of 355 residues. BioEdit software was used to compare the PgPrx3 amino acid sequence with other plants that showed homologies with Spinacia oleracea (70%), Vigna angularis (71%), Nicotiana tabacum (69%) and Linum usitatissimum (65%). The peroxidase of Vigna angulatis was the most homologous with ginseng. The chimeric PgPrx3 gene, 35S/35S/AMV/peroxidas/ Tnos, was constructed in the binary vector pRD400. Agrobacterium tumefaciens strain LBA4404 harboring disarmed Ti-plasmid was used to transform Nicotiana tabacum L. using the leaf disc. Incorporation of the chimeric gene PgPrx3 into plants were confirmed by PCR analysis of genomic DNA and RT-PCR analysis of mRNA from transgenic tobacco. The PgPrx3 gene from ginseng was stably expressed in transgenic tobacco plants.

당뇨병성 말초신경병의 평가에 대한 전류감지검사의 임상적 유용성

김승현,심충섭,김주한,김명호 대한신경과학회 1998 대한신경과학회지 Vol.16 No.5

제주 지역의 B형 간염 바이러스 유전자형

조지현,박도심,김태현,염주진,김학철,문주현,양재식 대한간학회 2004 Clinical and Molecular Hepatology(대한간학회지) Vol.10 No.1

목적: HBV 감염이 무증상의 자연 회복부터 간경변 혹은 간세포성 간암까지 다양하게 나타나는 요인들로 숙주의 요인과 바이러스의 요인으로 구분되어 제시되는데, 바이러스 요인으로는 감염된 바이러스이 유형이나 염기 변이들이 논의된다. HBV 유형을 구분하는 데는 혈청형과 유전자형이 이용되는데 근자에는 주로 유전자형이 이용된다. 이러한 HBV의 분포는 지역적으로 다르나 지역에 따라서 다른 유형들이 혼재되어 있다. 동남아시아는 B형이지만 극동아시아는 C형으로 알려져 있으며, 대만과 오키나와에서는 B와 C형이 혼재되어 나타난다. 이러한 결과로 미루어 보아 우리 나라의 남부지역에서는 B과 C형이 혼재되어 있을 가능성이 있다. 이에 본 연구는 제주 지역에서 HBV의 유전자형을 조사하고자 하였다. 대상과 방법: 원적지가 제주도이면사, HBsAg가 양성이었던 107명(평균 연령: 46.5세)을 대상으로 하였다. 이들의 혈청으로부터 HBsAg/antiHBe를 분석하고, DNA를 추출하여 7개의 유전자형에 따라 특이성을 갖는 시발체(genotype specific primer)들을 이용하여 PCR로 증폭한 후 이를 분석하였다. 주된 유전자형의 일부와 다른 유형에서 증폭에 이용되었던 시발체를 다르게 이용하여 hemi nested PCR로 증폭한 후 염기를 분석하여 계통발생학적 분석법을 이용하였다. 결과: 1. PCR에 의한 HBV DNA 양성은 107명 중 59명(55.1%)이 양성이었다. HBeAg/anti HBe의 발현 양태에 따라 음성/음성에서 2명(66.7%), 음성/양성에서 30명(41.1%), 양성/음성에서 24명(85.7%), 양성/양성에서는 3명(100%)에서 증폭되었다. 2. Multiplex PCR에서는 58명의 유전자형이 C형, 1명에서는 B형과 C형이 동반 출현하였다. 3. Genotype specific primer를 이용하여 각각 따로 PCR을 시행한 경우에 A형에 의하여 증폭된 예가 1예, B형에 의하여 증폭된 예가 1예, C형에 의하여 증폭된 예가 59예로 A형과 B형 모두가 C형에서 증폭된 예였다. 4. 한 쌍의 시발체에 의하여 증폭되었던 A형과 B형 및 C형의 일부에서 염기를 분석한 결과 모두 C 유전자형이었다. 결론: HBsAg이 양성인 제주 지역 주민의 HBV의 유전자형은 C형일 것으로 생각된다. Background/Aims: Hepatitis B virus (HBV) was classified into 8 genotypes by a sequence divergence in the entire genome designated from A o H. HBV genotypes have distinct geographic distributions. Recently, HBV genotypes have been partially found as influencing the clinical manifestation of chronic liver disease in hosts. In Korea, the distribution of HBV genotypes remains unclear. The aim of this study was to evaluate the prevalence of the HBV genotype on Jeju Island. Methods: Hepatitis B virus genotypes were evaluated among 107 hepatitis B carriers residing on Jeju Island. We used single PCR and multiplex-PCR assay with genotype-specific primer pairs for HBV genotypes A-F for the genotyping. Results: 1. Fifty nine samples (55%) were positive for HBV DNA. The positivity was different according to the pattern of HBeAg/anti-HBe expression, as -/-; 2/3 (66.7%), -/+; 30/73 (30%), +/-; 24/28 (85.7%) and +/+; 3/3 (100%). 2. In the single primer set of genotype-specific PCR, 59 samples (100%) were detected as genotype C and 2 (3%) were also detected as genotype A and B. 3. In multiplex-PCR, 58 samples (98%) were detected as genotype C and only one (2%) as a mixed pattern of genotype B and C. 4. When the PCR products were amplified with universal sense and genotype specific anti-sense from one genotype A, one B, and 2 C, all were included in genotype C. Conclusions: These results suggest that on Juju Island, almost all HBV genotypes are C.(Korean J Hepatol 2004;10:42-50)

Insecticide Resistance Conferred by MACE and MtoL Mutation in Para Based Mechanisms of the Myzus Persicae (Hemiptera: Aphididae)

Ju Il Kim,Min Kwon,Jae dong Shim,Jeom Soon Kim,Yong Gyu Lee,Si Hyeock Lee 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.05

The green peach aphid, Myzus persicae (Sulzer), is one of the most serious pest in cabbage cultivation. Field survey was carried out to know the insecticide resistance levels in five main cabbage cultivation regions (Pyeong-chang, Hong-cheon, Bong-wha, Mu-ju and Je-ju) in 2009~2011. The green peach aphid can resist a wide range of insecticides in five surveyed local populations. Among the nine tested insecticides, four chemicals (methomyl, bifenthrin, pymetrozine and flonicarmid) showed less than 60% mortality in the recommended concentration in most years of local populations. Multi resistant (MR) strain was selected from these populations and modified AChE (MACE: StoF mutation), MtoL mutation in para-type sodium channel, esterase over-expression were observed in almost of all populations including MR strain. Especially, StoF and MtoL mutations were highly correlated with resistance ratio and population based quantitative sequencing results. Therefore, these results suggested that molecular-biology based resistance monitoring can applied resistance management in M. persicae.

[PA-0077] Validating the reference genome assembly of Panax ginseng

Hyeonah Shim(Hyeonah Shim),Woohyeon Cho(Woohyeon Cho),Hyunjin Koo(Hyunjin Koo),Hye Ji Lee(Hye Ji Lee),Young Ju Oh(Young Ju Oh),Tae-Jin Yang(Tae-Jin Yang) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-