커뮤니티공간으로서의 어린이공원 조성에 관한 연구

김연금,김성주,박환지,유다희,최성용,홍승모,Kim, Yun-Geum,Kim, Seong-Joo,Park, Hwan-Ji,Yu, Da-Hee,Choi, Sung-Young,Hong, Seung-Mo 한국조경학회 2008 韓國造景學會誌 Vol.35 No.6

This study deals with the participatory design process of renovating a children's park. The process should proceed with two major elements. Firstly, the children's park should be designed from the perspective of children. Secondly, it should be designed in consideration of the context of the community. This study is divided into two parts. First is the review of theory concerning the meaning and roles of the children's park as a community space, the importance of children's participation in the design and the participatory method of the children. The second is the case study. The site for the case study is Ssial Park in Nowon-Gu, Seoul. The entire process of the case study is with community participation, from identifying the park's problems to the actual construction. At every step, through the participatory programs, the community participates in the decision making. In addition, mutual understanding among participants is pursued through diverse means of communication. The following results of the case study were found. Firstly, diverse participatory programs and the active trial to enable the community to communicate contribute to the sense of ownership and responsibility concerning the park Secondly, the community can negotiate the differences in opinions without needing the help of experts. Of course, there are limits in the case study, such as the fact that the community organization, which is the core of communication and maintenance concerning the park, is not organized through the process of a case study. Another is that more diverse methods, which inform all community members of the participatory process, should have been used.

균류 분화과정의 유전적 조절기작

박범찬,이환희,박윤희,박희문 충남대학교 생물공학연구소 2003 생물공학연구지 Vol.9 No.1

Fungi are well suited for studying mechanism controlling development and cell differentiation in multicellular eukaryotes. The asexual and sexual reproductive cycles are tightly coordinated during the life cycle of Aspergillus nidulans. The asexual (mitotic) reproductive cycle involves the formation of a number of differentiated cell types. Successful formation of multicellular reproductive structures and spores (conidia) requires the establishment of spatiotemporal gradients in essential transcriptional regulators, such as brlA, abaA and wetA. Altered gene expression is coordinated with critical changes in the movement of nuclei and the regulation of the cell division cycle. Therefore, it has been proposed to define a central regulatory pathway. Several early regulatory genes involved in signal transduction pathway controlling asexual sporulation are also required for activating the central pathway. Sexual (meiotic) reproduction is more complex, requiring the differentiation of several specialized tissue types. Although technical difficulties have limited efforts to study the genetics of sexual fruiting-body formation directly, some recent progress has been made. With the recent availability of mutants blocked in sexual development, understanding the genetic interactions between genes that function primarily in determining sexual sporulation will be achieved in the near future.

프리미돈의 한국인에서의 생체이용율시험

권광일,윤민혁,윤희열,박희찬,권준택,심희옥,김동출 충남대학교 약학대학 의약품개발연구소 2004 藥學論文集 Vol.19 No.-

A reversed phase HPLC method was developed and validated for the determination of primidone in human plasma. Propylthiouracil was used as an internal standard. Calibration curves were linear in the concentration range of 10-120 ng/㎖. The coefficient of variation of the intra- and inter-day precision were below 15%. The coefficient of variation of the accuracy were below 15% in the concentration range investigated. A bioavailability study was performed using the validated HPLC method. Twenty four healthy human male volunteers were orally administered 20 mg of primidone. The pharmacokinetic parameters were calculated using WinNonlin. The mean values of AUC_(LAST) was 85.0±17.7 ㎍ hr/㎖, C_(max) was 4.22±1.04 ㎍/㎖, T_(max) was 1.85±1.13 hr, t_(1/2) was 17.7±2.4 hr. The pharmacokinetic parameters and the HPLC method can be used for the desigm of bioequivalence study of primidone.

Elevated TRAF2/6 expression in Parkinson's disease is caused by the loss of Parkin E3 ligase activity.

Chung, Ji-Yun,Park, Hee Ra,Lee, Su-Jin,Lee, Sun-Hye,Kim, Jin Sik,Jung, Youn-Sang,Hwang, Sang Hyun,Ha, Nam-Chul,Seol, Won-Gi,Lee, Jaewon,Park, Bum-Joon United States and Canadian Academy of Pathology [e 2013 Laboratory investigation Vol.93 No.6

<P>Parkinson's disease (PD) is the second leading neurodegenerative disease, and is known to be induced by environmental factors or genetic mutations. Among the verified genetic mutations of PD, Parkin, isolated from the PARK2 locus, shows an autosomal recessive inheritance pattern and is known to be an E3 ligase. However, the physiological target of Parkin and the molecular mechanism of Parkin-deficiency-induced PD have not been clearly demonstrated until now. It has recently been proposed that inflammation, suggesting as a causal factor for PD, is enhanced by Parkin deficiency. Thus, we examined the relationship between inflammation-related factors and Parkin. Here, we provide the evidence that Parkin suppresses inflammation and cytokine-induced cell death by promoting the proteasomal degradation of TRAF2/6 (TNF-α receptor-associated factor 2/6). Overexpression of Parkin can reduce the half-lives of TRAF2 and TRAF6, whereas si-Parkin can extend them. However, mutant Parkins did not alter the expression of TRAF2/6. Thus, loss of Parkin enhances sensitivity to TNF-α- or IL-1β-induced JNK activation and NF-κB activation. Indeed, si-Parkin-induced apoptosis is suppressed by the knockdown of TRAF6 or TRAF2. We also observed elevated expression levels of TRAF6 and a reduction of IκB in an 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced PD mouse model. Moreover, elevated expression levels or aggregation of TRAF6 were detected in approximately half of the human PD tissues (7/15 cases) and 2 cases, respectively. In addition, TRAF6 and Parkin expression levels show a reverse relationship in human PD tissues. Our results strongly suggest that the reduction of Parkin or overexpression of TRAF2/6 by chronic inflammation would be the reason for occurrence of PD.</P>

투사각θ로 던진 투사체의 3차원 Mathematica Simulation

윤희중,박종엽 목원대학교 자연과학연구소 2001 自然科學 硏究論文集 Vol.10 No.2

이 논문에서는 Mathematica 4.1을 사용해서 투사각θ로 던진 투사체의 3차원 운동을 simulation하였다. 포사체 운동은 물리학의 기본이 되는 운동으로 물리학에 대한 기본적인 지식이 있는 사람이면 쉽게 이해할 수 있으나 Mathematica를 이용하여 프로그램을 작성하여 simulation해보면 포사체 운동을 좀 더 확실하게 이해 할 수 있다. 이 논문에서는 Mathematica를 간략하게 소개하고 Mathematica에서 포사체 운동을 simulation하여 대학 물리교육 현장에 적용한 과정을 제시하였다. Three demensional Mathematica simulation of the projectile with evaluation angle θ is presented. With this simulation, students can understand the motion of projectile more easily. We show interactive learning programs as examples to illustrate the concepts of projectile motion. We found that integrating the interactive Mathematica programs into physics class was very effective for improving the teaching and learning of physics in the undergraduate curriculum and for enhancing the ingenuity of students.

정신분열증에서 신경증적 증상의 임상적 의의에 관한 연구

박병관,신석철,강민희,신윤오 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.1

To investigate the clinical significance of the neurotic symptoms in schizophrenia, the author applied PSE(Present State Examination) on Screening Schedule to 153 schizophrenic patients who were fit for the aim of this study. The patients were divided into two groups to grasp the phenomenological characteristics as the group with neurotic symptoms (100 cases) and the group without neurotic symptoms(53 cases). Among 91 items except neurotic symptoms in PSE, 14 items were selected as significant under X²-test, and tried factor analysis with 14 items using SPSS/PC+. The results of this study were summarized as follows. 1. In the group with neurotic symptoms, 4 factors were extracted as slowness of speech and behavior and blunted affect factor, inappropriate answer and affect factor, delusion factor, and restricted and poverty of speech factor. In the group without neurotic symptoms, 5 factors were extracted as slowness speech and behavior and incoherence factor, inappropriate and poverty speech and self-neglect factor, delusion factor, inappropriate affect factor, and loss of affect factor. In the group of total patients, 4 factor were extracted as restricted and slowness of speech and behavior and blunted affect factor, delusion factor, inappropriate and poverty of speech and inappropriate affect factor, and incoherence factor. 2. In the group with neurotic symptoms, slowness of speech and behavior, inappropriate answer, blunted and inappropriate affect were the major symptoms and inappropriate answer and affect, delusion, restricted and poverty of speech were the minor symptoms. Among these symptoms, slowness restriction, poverty of speech and behavior, inappropriate answer and characteristic blunted affect were characterized by negative symptoms(i.e. defect state) which were frequent in chronic schizophrenia. 3. In the group without neurotic symptoms, slowness of speech and behavior, incoherence, and self-neglect were the major symptoms and inappropriate and poverty of speech, delusion, inappropriate and loss of affect were the minor symptoms. Among these symptoms, slowness and inappropriateness of speech and behavior, and characteristic incoherence and self-neglect were characterized by positive symptoms which were interpreted as less distorted reality than the group with neurotic symptoms in the process of schzophrenia. 4. In the group of total patients, restricted and slowness of speech and behavior, blunted affect and delusion were the major symptoms and inappropriateness, poverty and incoherence of speech, and inappropriate affect were the minor symptoms, which were composed of the major and the minor symptoms of the group with neurotic symptoms and without neurotic symptoms. These symptoms can be seen frequently in the diagnostic criterias and clinical practice and thought to be the result of the major symptoms common in schizophrenia regardless of he presence of neurotic symptoms.

Candida albicans의 형태변환 조절과정 규명의 최근 연구 동향

박윤희,박희문 충남대학교 생물공학연구소 2009 생물공학연구지 Vol.15 No.-

The opportunistic fungal pathogen Candida albicans can grow as yeast, pseudohyphae or true hyphae, and morphology of each cell is essential for efficient dissemination and tissue invasion. Therefore the ability to switch in C. albicans is thought to be an important virulence factor. The morphological conversion of C. albicans can be triggered in vitro by a various environmental stimuli including serum, pH, temperature, and nutrient depletion. These inducers regulate the expression of hypha-specific genes, which are involved in morphogenesis, through multiple signaling pathways such as MAPK pathway and cAMP/PKA pathway. In order to investigate Signal transduction pathways, disruption or over-expression of the component gene(s) were performed and then change(s) in morphological transition as well as the expression of hypha-specific genes are observed in general. As the approach to unravel new roles of already-known signaling pathways in fungal pathogenicity, responses toward external stimuli such as hypoxic condition and iron utilization are performed these days. The new approaches revealed that the morphological conversion in C. albicans is resulted from the consorted action of multiple signaling pathways rather than from a single pathway. Consequently, the existence of a novel Signaling pathway for dimorphic switch in C. albicans could not be excluded. In this context, it is noteworthy that the involvement of LAMMER kinase in fungal development; LAMMER kinase is known to be involved in oxidative-stress response, filamentous growth, and flocculation in fission yeast; S. cerevisiae LAMMER kinase, ScKNS1, is also reported to phosphorylate Slt2, MAPK of cell wall integrity pathway, and to interact with dual-specificity phosphatase Sdp1, which regulates the activity of Slt2. Therefore, studies on the function of LAMMER kinase in C. albicans will be helpful to reveal novel regulatory mechanisms for virulence in pathogenic fungi.

水銀, 카드뮴, 크롬이 흰쥐의 肝에 미치는 影響

尹惠禎,朴熙聖 梨花女子大學校 韓國生活科學硏究院 1982 韓國生活科學硏究院 論叢 Vol.30 No.-

肝에 對한 重金屬의 影響을 알아보고자 水銀, 카드뮴, 크롬을 흰쥐의 腹腔內에 單回大量投與와 小量으로 1週, 2週, 3週間 每日 投與하여 肝에 畜積된 重金屬量, 總蛋白質量 變化 및 Thiobarbituric acid value를 測定하여 다음과 같은 結果를 얻었다. 가. 水銀投與群 畜積되는 水銀의 量은 投與量과 期間에 比例하여 增加하지는 않았다. 水銀中毒이 發生하는 時期에는 體重이 減少되었고, 肝의 蛋白質合成도 저해되었다. 水銀은 TBA價(脂肪의 peroxidation)에 영향을 미쳤다. 나. 카드뮴投與群 肝에 카드뮴이 어느 程度까지 畜積되면 더 以上 현저한 薯積作用은 없었다. 카드뮴 中毒 初期에는 蛋白質合成이 促進되었으나 肺에 카드뮴이 어느 程度 畜積되면 蛋白質合成이 저해되었다. 카드뮴은 脂肪의 peroxidation을 多小 저해하였다. 다. 크롬投與群 肝에 蓄積되는 크롬의 量은 投與期間에 따라 增加하였으며 正常群보다는 增加率이 낮았으나 體重이 增加하였다. 크롬中毒初期에는 肝의 蛋白質合成이 促進되었으나 그 以後는 현저히 저해되었다. 크롬은 脂肪의 peroxidation에 크게 영향을 미치지 않았다. Effect of the treated with Hg, Cd and Cr on metal distribution, protein synthesis and lipid peroxidation in the rat liver was studied. The results of this study were as follows: A. In treated with the Hg group: 1. The amount of Hg accmulated in liver was not always proportional to administration amount and time. 2. Initial and last time of Hg toxicity, protein synthesis was accelerated. 3. The body weights were reduced with Hg toxicity occuring. 4. Hg effected on lipid peroxidation. In the initial few days treated with Hg increased lipid peroxidation but after 2 weeks lipid peroxidation were inhibited markdly. B. In treated with the Cd group: 1. The amount of Cd accumulated in liver increased to 78.22±6.18㎍ within first 2 weeks but after more accumulation was not observed. 2. In the early time Cd toxicity protein synthesis was increased. 3. Lipid peroxidation was inhibited more or less by treated with Cd. C. In treated with the Cr group: 1. The amount of Cr accumulated in liver increased with treated time. 2. In the early time of Cd toxicity protein synthesis was accelerated. 3. Cr toxicity did not reduce body weight. 4. Cr did not effect on lipid peroxidation markdly.

편측 협응훈련에 의한 운동신경망의 재조직 및 교차훈련의 효과

박지원,김종만,서정환,김연희 한국전문물리치료학회 2002 한국전문물리치료학회지 Vol.9 No.3

We report the reorganization of motor network resulted from intensive unilateral coordination training and the effect of cross education on the untrained side in patient with traumatic brain injury using functional magnetic resonance imaging (fMRI). A 22 year-old male patient who had suffered from diffuse axonal injury for 58 months showed coordination deficit in the left hand at initial examination. Intensive motor training including complex finger movements and coordination activities using a metronome was introduced to the patient 4 hours per day for a week. FMRI was performed on a 3T ISOL Forte scanner. All functional images were analyzed using SPM-99 software. Hand function was improved after training not only in the trained lefr hand, but also in the untrained right hand. There was no activation in the right primary motor area (M1) during left hand movement before training whereas robust activation of left M1 was demonstrated by the right hand movement. Profuse activation of bilateral prefrontal lobes was seen during both hand movements before training. After training of left hand, right M1 became prominently activated during the left hand motion. The activation of bilateral prefrontal lobes disappeared after training not only for the left hand movement but also for the right, which clearly demonstrated the effect of cross education. This case report demonstrated the learning-dependent reorganization of the M1 and the effect of cross education.

Drosophila에서 Genistein의 항돌연변이 효과

윤희선,박건영,이원호 대한암예방학회 2000 Journal of cancer prevention Vol.5 No.3