서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1997)

김재홍,문득곤,김정수,김용준,임동진,박상훈,김희성,이민수,송기훈,김갑형,김형석,성소영,이인섭,김석우,황지환,조창근,김경문,부태성 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.3

Background : In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. Objective & Methods : For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results : In 1997. 99 strains of N. gonorrhoeae were isolated, among which 45(45.5%) were PPNG. Conclusion : The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 45.5% in 1997.

다중 중합효소 연쇄반응을 이용한 소의 Johne병 진단 기법 확립

김종배,송혜원,김근희,김홍,신광순,김두 대한의생명과학회 2000 Journal of biomedical laboratory sciences Vol.6 No.1

반추수에서 발생하는 Johne병의 조기 진단 방법을 제시하고 이 질병의 원인체와 미생물학적 특징이 유사한 M. bovis, M. avium 등의 mycobacteria감염증을 감별 진단하는 방법을 개발하기 위하여 Mycobacterium 균속의 표준균주를 사용하여 중합효소 연쇄반응을 확립하였다. Johne병으로 의심되는 소의 혈액과 유즙을 채취하여 분리한 단핵구 및 거식세포로부터 genomic DNA를 추출하였다. 각 시료로부터 추출한 DNA를 template로 이용하여 Mycobacterium spp.에 특이적인 16S rDNA primer set를 이용한 PCR을 수행하여 시료내의 mycobacterial DNA 보유 여부를 확인하였다. 한편 mycobacteria 양성으로 확인된 시료는 M. avium complex 균종에 특이한 16S rDNA 염기서열을 기초로하여 제작한 primer set와 M. paratuberculosis 의 IS900 sequence에 특이한 primer set를 이용하여 duplex PCR을 수행하여 Johne병 원인체의 보균 여부를 조사하였으며, 이 결과를 oligonucleotide probe를 사용한 Southern blot hybridization을 통하여 다시 확인하였다. 이와 같은 duplex PCR 기법을 실제 축산 현장에서 수집한 유즙과 말초혈액으로부터 분리한 단핵구 및 거식세포 시료에 적용한 결과 본 연구에서 확립한 duplex PCR기법 유용성을 확인할 수 있었다. Diagnosis of Bovine Johne's Disease Using Multiplex Polymerase Chain Reactions In order to improve the early diagnosis of Johne's disease in ruminants, duplex polymerase chain reaction system for the detection of the etiologic agent of M. paratuberculosis and for the differentiation of other mycobacterial animal pathogens, such as M. bovis and M. avium, was applied. Genomic DNAs were purified from peripheral blood monocytes or milk macrophages and were used as templates in the duplex PCR. Detection of Mycobacterium spp. in the specimen was carried out by PCR using primer set specific to the mycobacterial 16S rDNA. And then, mycobacterial DNA-positive specimens were further differentiated with duplex PCR system which was composed of primer sets specific to 16S rDNA of M. avium complex and IS900 gene of M. paratuberculosis. The results were re-confirmed by Southern blot hybridization with oligonucleotide specific to the internal sequence of IS900 PCR amplicons. The applicability of this duplex PCR system was evaluated with DNAs extracted from clinical specimens of peripheral blood monocytes and milk macrophages. In summary, the duplex PCR amplification system described in this experiment is promising molecular technique for the early diagnosis of Johne's disease in ruminants.

아시아티코사이드로부터 메칠 2β, 3β-에폭시-23-히드록시우르스-12-엔-28-오애이트의 합성 및 이의 환원적 에폭시환 개열반응

주상섭,임두연,서성기,남태규,박형근,김희두,김창민,이민희,백형근,이민정,정영훈 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

Steroidal 2β, 3β-epoxy compound was prepared from asiaticoside via six steps and reduced regioselectively with lithium aluminum hydride. Epoxide ring opening furnished 9 as a sole product at reflux condition through axial hydride attack at C-3.

토당귀 종자의 형태적 특성과 발아와 입모율에 대한 저장방법,생장조절제 및 프라이밍 처리 효과

안희정,안복주,·김도현,·안영섭,·김영국,박춘근,이상원,·박충범·차선우,·송범헌, 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.1

This study was conducted to have basic and applied informations to establish the cultivation method and to improve the cultivation techniques of Angelica gigas Nakai through investigating the rates of germination and seedling establishment with treatment of plant growth regulator and seed priming. The germination rates of round shape seed were about 0.3~12% higher than those of parallel shape. The germination rates with different storage durations were increased at the level of 25oC and 4oC with the storage days, while they were clearly higher at -20oC compared to those at 4oC and 25oC without the storage durations. With the results of germination rates with treating of plant growth regulator and seed priming, their rates with the round shape seeds were comparatively higher about 6% than those with parallel shape seed and they were increased with increasing of the storage durations. The germination rates were generally appeared higher with GA3 than did those with seed priming. The highest germination rate was appeared at 50 ppm GA3 of plant growth regulator and at -0.5 MPa PEG6000 of seed priming. The germination rates?ㅤ ?ㅤ of Angelica gigas were different with different temperature, storage durations and the treatments of plant growth regulator and seed priming. These results could be used according to different cultivating conditions.

랫드에서 법제유황과 비법제유황의 독성비교

김근수, 정유리, 이남진, 홍성희, 유종훈, 정재황, 강종구 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.2

본문참고

중추성 요붕증으로 발현된 랑거한스세포 조직구증식증 1례

김준성,윤원상,김근모,최영권,박기원,김병희,유용상 대한소아내분비학회 2004 Annals of Pediatirc Endocrinology & Metabolism Vol.9 No.1

척추 수술시 자가혈액 수혈기를 이용한 자가수혈의 효과

김명호,유문집,유석주,박진영,정홍근,박희곤,문기혁 단국대학교 2000 論文集 Vol.35 No.-

Study Design : Spine fusion with fixation procedure is associated with substantial operative blood loss, which frequently necessitates transfusion. The authors evaluated the safety and efficacy of intraoperative autotransfusion using Cell Saver during spinal surgery. Summary of Literature Review : It has been reported that the contraindications to the use of intraoperative autologous transfusion are malignancy, infection, and gross wound contamination. Objectives : The purpose of this study was to evaluate the safety and efficacy of intraoperative autotransfusion using Cell Saver during spine surgery. Material and Methods : We retrospectively reviewed 62 patients who had operation for lumbosacral spinal stenosis and hypotensive anesthesia. Among them, Group Ⅰwhich received autotrans fusion using Cell Savers included 26 patients with fusion of 2 segments, and Group Ⅱ as control group included 13 patients. Group Ⅲ which received autotransfusion using Cell Savers included 17 patients with function of 3 segments, and Group Ⅳ as control group included 6 patients. We analyzed the amount of intraoperative blood salvage using a Cell Saver, the amount of total homologous transfusion amount, intraoperative blood loss, the amount of postoperative drainage amount and perioperative changes of hemoglobin, hematocrit and platelet count. Results : There was no statistical significance between Cell-Saver group and control group in amount of total homologous transfusion and intraoperative(except in 2 segment of fusion using cell saver: 86.5% increased) & postoperative blood loss(p>0.05). The circulatory balance(amount of intraoperative blood salvage using a Cell Saver plus amount of total homologous transfusion minus amount of total blood loss) was statistical significance between Cell-Saver group and Control group. The Cell-Saver group had positive balance and Control group had negative balance. There were no statistical differance in the evolusion of hemoglobin, hematoocrit and platelet count between Cell-Saver group and Control group(p>0.05). There was no transfusion reaction and thromboembolic disease in all group. Conclusion : In retrospective study, Cell Savers was safe and immediate availability in orthopedic surgery such as spinal surgery. It was effective methods for storing a sufficient volume of blood for spine surgery without affecting the patient's postoperative hemodynamic ststes, too. It was possible to operation without intraoperative homologous blood transfusion in 2 or 3 segment fusion. We may need further study.

난분해성 폐수처리를 위해 Bioaugmentation한 생물막 반응기에 관한 연구

이건,김희,조순자,박근태,차미선,이상준 부산대학교 환경문제연구소 2002 環境硏究報 Vol.20 No.-

The effect on the stability of biofilm and phenol removal efficiency under high phenol loading was investigated by RBC(Rotating Biological Contactor) using augmented reactor and unaugmented reactor with Rhodococcus sp. EL-GT. The operation of RBCs was carried out at rotating speed of 10 rpm and hydraulic retention time of 7 h at 20℃. The maximum amount of dry weight of the biofilm was 0.04 g/㎠ to running time for 15 days. The amount of attached biofilm of contactors was stabilized as 0.15∼0.03 g/㎠ for 35 days' running times and that of two reactors was similar value. But unaugmented reactor was shocked at 1,200 mg/ℓ phenol loading that the biofilm and removal efficiency were impacted. The bioaugmentation reactor showed higher removal rates than unaugmented reactors about phenol, sCOD and nitrogen contents. Bioaugmentation, with the strain originating from the same ecosystem and growing effectively on a selective substrate, was often effective means to treatment of recalcitrants like as phenol wastewater.

오리엔탈나리의 球根 收穫時期와 低溫處理 期間이 生育에 미치는 影響

金熙峻,金正萬,金致善,柳汀,崔泳根,文炳永 한국화훼연구회 2001 화훼연구 Vol.9 No.1

This study was carried out to determine the effect of the duration of chilling treatment and the lifting time of bulbs on growth of Lilium Oriental hybrids for product of cut flower in the type of forcing or semiforcing culture in Korea. "Casablanca", "Acapulco" and "Le Reve" in lilium Oriental hybrids were used in these experiment and carried out from 1999 to 2000 in Iksan, Korea. The bulbs for experiment were lifted on August 27, September 27 and October 27 and the all of them were chilled at 5±1℃ for 0,8,10,12 weeks. Development of the bulbs lifted on October 27 was superior than that of lifted bulbs on August 27 in the three cultivars. The bulbs lilted lately and chilled for a long time were sprouted more earlier and grown faster. In order to increase bulb sprouting until 100%, the duration of chilling treatment of bulbs was need for 12 weeks on August 27, 10 weeks on September 27 and above 8 weeks on October 27. The flowering according to the duration of chilling treatment and the lifting time of bulbs was same above. Consequently, the bulbs should be chilled at 5±1℃ for chilled for 10 weeks at late September and 8 weeks in producted bulbs at late October in earlyㆍMidseason variety fur product of cut flower hut bulbs of late variety should be chilled for 12 weeks at late September and 10 weeks at late October.

Monascus anka로부터 유기배양에 의한 적색소의 대량생산

김희구,박근태,손홍주 한국식품영양학회 1998 韓國食品營養學會誌 Vol.11 No.6

Monascus anka KFCC 4478의 적색소 생산력을 증가시키기 위하여 발효조 배양조건을 검토한 후, 탄소원을 연속적 또는 간헐적으로 공급하는 유기배양을 하였다. 5l 용량의 발효조를 이용하여 배양시 교반속도 200rpm, 통기량 1vvm 및 종균 접종량 10%(v/v)일 때 색소 생성량이 최대였다. 또한 벽면에 부착된 균사체를 재접종함으로써 색소 생성력이 향상되었다. Intermittent feeding fed-batch culture에 의하여 배양 9일만에 30g/l의 건조균체와 350(A_495)의 색소가 생성되었으며, continuous feeding fed-batch culture에 의하여 22g/l의 건조균체와 190(A_495)의 색소가 얻어졌다. 따라서 본 균주에 의하여 적색소를 생산코자 할 경우는 회분배양보다 유기배양이 더 우수한 것으로 나타났다. The production of red pigment from glucose by fed-batch culture of Monascus anka was investigated. In batch culture using fermentor, 200 rpm of agitation speed, 1vvm of aeration volume, and 10%(v/v) of inoculum size were optimal, respectively. The red pigment production was increased by removal of wall-attached mycelium. In an intermittent feeding fed-batch culture, dry cell weight increased to 30g/l, and the red pigment content reached 350 of absorbance at 495nm. In a continuous feeding fed-batch culture, dry cell weight increased to 22g/l, and the red pigment content reached 190 of absorbance at 495nm.